Restriction map for
pFN21AE0808
(Circular) (Six-base) MAPSORT of: pFN21AE0808.seq Check: 1257 from: 1 to: 5548
pFN21AE0808 5548 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3358 278
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2391 2891 1937
Size: 454 500 4594
Fragments arranged by size:
4594 500 454
Acc65I G'GTAC_C
Cuts at: 2869 2869
Size: 5548
AclI AA'CG_TT
Cuts at: 1563 3675 4048 1563
Size: 2112 373 3063
Fragments arranged by size:
3063 2112 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3611 4995 5263 1632
Size: 1979 1384 268 1917
Fragments arranged by size:
1979 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5351
AflIII A'CryG_T
Cuts at: 1203 4447 5424 1203
Size: 3244 977 1327
Fragments arranged by size:
3244 1327 977
AgeI A'CCGG_T
Cuts at: 4379 4379
Size: 5548
AhdI GACnn_n'nnGTC
Cuts at: 4277 4277
Size: 5548
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5548
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5548
AlwNI CAG_nnn'CTG
Cuts at: 2141 2240 3291 4863 2141
Size: 99 1051 1572 2826
Fragments arranged by size:
2826 1572 1051 99
ApaLI G'TGCA_C
Cuts at: 3605 4761 3605
Size: 1156 4392
ApoI r'AATT_y
Cuts at: 1493 2857 3083 3235 5344 1493
Size: 1364 226 152 2109 1697
Fragments arranged by size:
2109 1697 1364 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4102 160
Size: 872 3070 1606
Fragments arranged by size:
3070 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
AvaI C'yCGr_G
Cuts at: 1870 1943 2873 1870
Size: 73 930 4545
Fragments arranged by size:
4545 930 73
BamHI G'GATC_C
Cuts at: 2878 3245 2878
Size: 367 5181
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2869 4324 618
Size: 325 217 627 1082 1455 1842
Fragments arranged by size:
1842 1455 1082 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2079 2867 1391
Size: 688 788 4072
Fragments arranged by size:
4072 788 688
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2580 2795 928
Size: 971 681 215 3681
Fragments arranged by size:
3681 971 681 215
BbvCI CC'TCA_GC
Cuts at: 2107 2107
Size: 5548
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3739 3773 1903
Size: 34 1802 34 3678
Fragments arranged by size:
3678 1802 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3440 4650 3440
Size: 1210 4338
BclI T'GATC_A
Cuts at: 1548 2049 1548
Size: 501 5047
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2462 4159 136
Size: 107 122 71 631 605 790 1697 1525
Fragments arranged by size:
1697 1525 790 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5543 5543
Size: 5548
BlpI GC'TnA_GC
Cuts at: 2965 2965
Size: 5548
Bme1580I G_kGCm'C
Cuts at: 1790 2757 3609 4765 1790
Size: 967 852 1156 2573
Fragments arranged by size:
2573 1156 967 852
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5548
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3279 4237 442
Size: 416 53 83 399 24 1862 958 1753
Fragments arranged by size:
1862 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4208 1239
Size: 2969 2579
Bpu10I CC'TnA_GC
Cuts at: 2107 2107
Size: 5548
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3673 4538 4836 3673
Size: 865 298 4385
Fragments arranged by size:
4385 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4211 882
Size: 617 2712 2219
Fragments arranged by size:
2712 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2622 3339 493
Size: 711 1418 717 2702
Fragments arranged by size:
2702 1418 717 711
BsaBI GATnn'nnATC
Cuts at: 5542 5542
Size: 5548
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3355 3737 4372 275
Size: 53 83 186 936 1822 382 635 1451
Fragments arranged by size:
1822 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3981 4379 4653 4800 5211 3981
Size: 398 274 147 411 4318
Fragments arranged by size:
4318 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5323
Fragments arranged by size:
5323 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5548
BseYI C'CCAG_C
Cuts at: 2662 4751 5247 2662
Size: 2089 496 2963
Fragments arranged by size:
2963 2089 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4975
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2998 3759 4787 1540
Size: 275 186 997 761 1028 2301
Fragments arranged by size:
2301 1028 997 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2445 3609 3694 4765 2445
Size: 1164 85 1071 3228
Fragments arranged by size:
3228 1164 1071 85
BsmI GAATG_Cn'
Cuts at: 2666 3059 3152 2666
Size: 393 93 5062
Fragments arranged by size:
5062 393 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2445 2757 2867 3609 3694 4765
Size: 399 289 366 312 110 742 85 1071
Cuts at: 4765 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 366 312 289 110
85
BspEI T'CCGG_A
Cuts at: 5211 5211
Size: 5548
BspHI T'CATG_A
Cuts at: 2052 3437 5167 2052
Size: 1385 1730 2433
Fragments arranged by size:
2433 1730 1385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2903 844
Size: 2059 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3435 1394
Size: 429 1612 3507
Fragments arranged by size:
3507 1612 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2262 2451 4043 4217 65
Size: 1183 1014 189 1592 174 1396
Fragments arranged by size:
1592 1396 1183 1014 189 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4192 4379 1700
Size: 110 2382 187 2869
Fragments arranged by size:
2869 2382 187 110
BsrGI T'GTAC_A
Cuts at: 96 2007 96
Size: 1911 3637
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5548
BssSI C'ACGA_G
Cuts at: 3608 4620 3608
Size: 1012 4536
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2240 794
Size: 1446 4102
BstBI TT'CG_AA
Cuts at: 4352 4352
Size: 5548
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5548
BstXI CCAn_nnnn'nTGG
Cuts at: 2693 2693
Size: 5548
BstYI r'GATC_y
Cuts at: 1972 2878 3245 3630 3647 5099 5185 5197 5543
Size: 906 367 385 17 1452 86 12 346
Cuts at: 5543 1972
Size: 1977
Fragments arranged by size:
1977 1452 906 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2392 2392
Size: 5548
Bsu36I CC'TnA_GG
Cuts at: 2142 2142
Size: 5548
BtgI C'CryG_G
Cuts at: 513 1065 2172 513
Size: 552 1107 3889
Fragments arranged by size:
3889 1107 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2672 487
Size: 683 56 1446 3363
Fragments arranged by size:
3363 1446 683 56
BtsI GCAGTG_nn'
Cuts at: 2580 3064 3858 3878 2580
Size: 484 794 20 4250
Fragments arranged by size:
4250 794 484 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5548
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5513
DraI TTT'AAA
Cuts at: 2852 2852
Size: 5548
DraIII CAC_nnn'GTG
Cuts at: 2575 2575
Size: 5548
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4555 809
Size: 507 3239 1802
Fragments arranged by size:
3239 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2277 2995 3884
Size: 54 1425 50 275 18 447 718 889
Cuts at: 3884 5513 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 718 447 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2995 1537
Size: 275 1183 4090
Fragments arranged by size:
4090 1183 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2093 3478 1353
Size: 740 1385 3423
Fragments arranged by size:
3423 1385 740
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4135 4509 4655 387
Size: 1064 138 2546 374 146 1280
Fragments arranged by size:
2546 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3611 4208 4995 5263 1239
Size: 393 1979 597 787 268 1524
Fragments arranged by size:
1979 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2611 2611
Size: 5548
EcoRI G'AATT_C
Cuts at: 2857 3235 2857
Size: 378 5170
FspI TGC'GCA
Cuts at: 4054 4054
Size: 5548
HaeII r_GCGC'y
Cuts at: 1678 4695 1678
Size: 3017 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4189 4221 4263 4295 1373
Size: 32 135 32 2617 32 42 32 2626
Fragments arranged by size:
2626 2617 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2892 3138 669
Size: 988 281 954 246 3079
Fragments arranged by size:
3079 988 954 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5548
HpaI GTT'AAC
Cuts at: 3138 3138
Size: 5548
KpnI G_GTAC'C
Cuts at: 2873 2873
Size: 5548
MfeI C'AATT_G
Cuts at: 3147 3147
Size: 5548
MluI A'CGCG_T
Cuts at: 5424 5424
Size: 5548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4662 4846 1305
Size: 3357 184 2007
Fragments arranged by size:
3357 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2279 10
Size: 54 1425 343 447 3279
Fragments arranged by size:
3279 1425 447 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2228 2637 2648 2729 3506 3865 4024
Size: 648 1062 409 11 81 777 359 159
Cuts at: 4024 518
Size: 2042
Fragments arranged by size:
2042 1062 777 648 409 359 159 81
11
MspA1I CmG'CkG
Cuts at: 1606 2129 2345 2964 3641 4789 5034 1606
Size: 523 216 619 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 619 523 245 216
NcoI C'CATG_G
Cuts at: 513 1065 2172 513
Size: 552 1107 3889
Fragments arranged by size:
3889 1107 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5548
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5548
NotI GC'GGCC_GC
Cuts at: 2995 2995
Size: 5548
NspI r_CATG'y
Cuts at: 1132 1229 2906 4451 1132
Size: 97 1677 1545 2229
Fragments arranged by size:
2229 1677 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5548
PciI A'CATG_T
Cuts at: 4447 4447
Size: 5548
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2173 1108
Size: 1065 4483
PmeI GTTT'AAAC
Cuts at: 2852 2852
Size: 5548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3617 3649 5130 5162 3617
Size: 32 1481 32 4003
Fragments arranged by size:
4003 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5548
PsiI TTA'TAA
Cuts at: 3118 3118
Size: 5548
PstI C_TGCA'G
Cuts at: 830 2137 2900 830
Size: 1307 763 3478
Fragments arranged by size:
3478 1307 763
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5548
PvuII CAG'CTG
Cuts at: 2345 2345
Size: 5548
SalI G'TCGA_C
Cuts at: 1936 2890 1936
Size: 954 4594
SbfI CC_TGCA'GG
Cuts at: 2900 2900
Size: 5548
ScaI AGT'ACT
Cuts at: 3796 5508 3796
Size: 1712 3836
SfcI C'TryA_G
Cuts at: 826 1045 2133 2520 2896 4031 4712 4903 826
Size: 219 1088 387 376 1135 681 191 1471
Fragments arranged by size:
1471 1135 1088 681 387 376 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5548
SmaI CCC'GGG
Cuts at: 1872 2875 1872
Size: 1003 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3652 4553 4815 820
Size: 197 926 1709 901 262 1553
Fragments arranged by size:
1709 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3339 493
Size: 2846 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5548
SphI G_CATG'C
Cuts at: 1132 2906 1132
Size: 1774 3774
SspI AAT'ATT
Cuts at: 5 52 3472 5
Size: 47 3420 2081
Fragments arranged by size:
3420 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5548
StyI C'CwwG_G
Cuts at: 513 1065 2172 2987 513
Size: 552 1107 815 3074
Fragments arranged by size:
3074 1107 815 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3575 3592 3745 3930 4375 325
Size: 1255 1995 17 153 185 445 1498
Fragments arranged by size:
1995 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 3794 5506
Size: 274 80 33 51 1443 30 1787 1712
Cuts at: 5506 96
Size: 138
Fragments arranged by size:
1787 1712 1443 274 138 80 51 33
30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5508 5540 5508
Size: 32 5516
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5548
XbaI T'CTAG_A
Cuts at: 2884 2884
Size: 5548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5548
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5548
XmaI C'CCGG_G
Cuts at: 1870 2873 1870
Size: 1003 4545
XmnI GAAnn'nnTTC
Cuts at: 3677 3677
Size: 5548
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3356 276
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI