Restriction map for
pFN21AE0807
(Circular) (Six-base) MAPSORT of: pFN21AE0807.seq Check: 7343 from: 1 to: 5788
pFN21AE0807 5788 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3598 278
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2160 3131 1937
Size: 223 971 4594
Fragments arranged by size:
4594 971 223
Acc65I G'GTAC_C
Cuts at: 3109 3109
Size: 5788
AclI AA'CG_TT
Cuts at: 1563 3915 4288 1563
Size: 2352 373 3063
Fragments arranged by size:
3063 2352 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3851 5235 5503 1632
Size: 2219 1384 268 1917
Fragments arranged by size:
2219 1917 1384 268
AfeI AGC'GCT
Cuts at: 2249 2249
Size: 5788
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5591
AflIII A'CryG_T
Cuts at: 1203 4687 5664 1203
Size: 3484 977 1327
Fragments arranged by size:
3484 1327 977
AgeI A'CCGG_T
Cuts at: 4619 4619
Size: 5788
AhdI GACnn_n'nnGTC
Cuts at: 4517 4517
Size: 5788
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5788
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2396 2435 2438 2456 1184
Size: 1212 39 3 18 4516
Fragments arranged by size:
4516 1212 39 18 3
AlwNI CAG_nnn'CTG
Cuts at: 2551 3019 3531 5103 2551
Size: 468 512 1572 3236
Fragments arranged by size:
3236 1572 512 468
ApaI G_GGCC'C
Cuts at: 2533 2533
Size: 5788
ApaLI G'TGCA_C
Cuts at: 2381 2441 3845 5001 2381
Size: 60 1404 1156 3168
Fragments arranged by size:
3168 1404 1156 60
ApoI r'AATT_y
Cuts at: 1493 2626 3097 3323 3475 5584 1493
Size: 1133 471 226 152 2109 1697
Fragments arranged by size:
2109 1697 1133 471 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4342 160
Size: 872 3310 1606
Fragments arranged by size:
3310 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
AvaI C'yCGr_G
Cuts at: 1870 1943 2082 2770 3113 1870
Size: 73 139 688 343 4545
Fragments arranged by size:
4545 688 343 139 73
AvrII C'CTAG_G
Cuts at: 2194 2452 2194
Size: 258 5530
BamHI G'GATC_C
Cuts at: 3118 3485 3118
Size: 367 5421
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2513 3109 4564 618
Size: 325 217 627 726 596 1455 1842
Fragments arranged by size:
1842 1455 726 627 596 325 217
BanII G_rGCy'C
Cuts at: 1391 2062 2533 3107 1391
Size: 671 471 574 4072
Fragments arranged by size:
4072 671 574 471
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4817
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3979 4013 1903
Size: 34 50 34 1958 34 3678
Fragments arranged by size:
3678 1958 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3680 4890 3680
Size: 1210 4578
BclI T'GATC_A
Cuts at: 1548 2728 1548
Size: 1180 4608
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2913 4399 136
Size: 107 122 71 631 605 1241 1486 1525
Fragments arranged by size:
1525 1486 1241 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3080 5783 3080
Size: 2703 3085
BlpI GC'TnA_GC
Cuts at: 3205 3205
Size: 5788
Bme1580I G_kGCm'C
Cuts at: 1790 2385 2445 2516 2533 2833 3849 5005 1790
Size: 595 60 71 17 300 1016 1156 2573
Fragments arranged by size:
2573 1156 1016 595 300 71 60 17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5788
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2958 3519 4477
Size: 416 53 83 399 24 1541 561 958
Cuts at: 4477 442
Size: 1753
Fragments arranged by size:
1753 1541 958 561 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5788
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2081 2095 3023 2081
Size: 14 928 4846
Fragments arranged by size:
4846 928 14
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 2272 2528 2996 4448 1239
Size: 851 182 256 468 1452 2579
Fragments arranged by size:
2579 1452 851 468 256 182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3913 4778 5076 3913
Size: 865 298 4625
Fragments arranged by size:
4625 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2132 4451 882
Size: 617 633 2319 2219
Fragments arranged by size:
2319 2219 633 617
BsaAI yAC'GTr
Cuts at: 493 1204 3579 493
Size: 711 2375 2702
Fragments arranged by size:
2702 2375 711
BsaBI GATnn'nnATC
Cuts at: 5782 5782
Size: 5788
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3595 3977 4612 275
Size: 53 83 186 936 2062 382 635 1451
Fragments arranged by size:
2062 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2487 2785 4221 4619 4893 5040 5451 2487
Size: 298 1436 398 274 147 411 2824
Fragments arranged by size:
2824 1436 411 398 298 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2038 2068 1178
Size: 30 165 30 635 30 4898
Fragments arranged by size:
4898 635 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2754 2999 1186
Size: 1568 245 3975
Fragments arranged by size:
3975 1568 245
BseYI C'CCAG_C
Cuts at: 2509 3050 4991 5487 2509
Size: 541 1941 496 2810
Fragments arranged by size:
2810 1941 541 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5215
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3238 3999 5027 1540
Size: 275 186 1237 761 1028 2301
Fragments arranged by size:
2301 1237 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2062 2385 2445 3849 3934 5005 2062
Size: 323 60 1404 85 1071 2845
Fragments arranged by size:
2845 1404 1071 323 85 60
BsmI GAATG_Cn'
Cuts at: 3299 3392 3299
Size: 93 5695
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2062 2385 2445 2516 2533 2833 3107
Size: 399 272 323 60 71 17 300 274
Cuts at: 3107 3849 3934 5005 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 323 300 274 272
85 71 60 17
BspEI T'CCGG_A
Cuts at: 2785 5451 2785
Size: 2666 3122
BspHI T'CATG_A
Cuts at: 2749 3677 5407 2749
Size: 928 1730 3130
Fragments arranged by size:
3130 1730 928
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2304 2438 3143 844
Size: 1460 134 705 3489
Fragments arranged by size:
3489 1460 705 134
BsrBI CCG'CTC
Cuts at: 1394 1823 3675 1394
Size: 429 1852 3507
Fragments arranged by size:
3507 1852 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4283 4457 65
Size: 1183 3035 174 1396
Fragments arranged by size:
3035 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4432 4619 1700
Size: 110 2622 187 2869
Fragments arranged by size:
2869 2622 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5788
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5788
BssSI C'ACGA_G
Cuts at: 3848 4860 3848
Size: 1012 4776
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5788
BstBI TT'CG_AA
Cuts at: 4592 4592
Size: 5788
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5788
BstXI CCAn_nnnn'nTGG
Cuts at: 2272 2798 2272
Size: 526 5262
BstYI r'GATC_y
Cuts at: 1972 3080 3118 3485 3870 3887 5339 5425 5437
Size: 1108 38 367 385 17 1452 86 12
Cuts at: 5437 5783 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1108 385 367 346 86 38
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5236
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5049
Fragments arranged by size:
5049 683 56
BtsI GCAGTG_nn'
Cuts at: 3304 4098 4118 3304
Size: 794 20 4974
Fragments arranged by size:
4974 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5788
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5753
DraI TTT'AAA
Cuts at: 3092 3092
Size: 5788
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4795 809
Size: 507 3479 1802
Fragments arranged by size:
3479 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2289 2590 3235
Size: 54 1425 50 275 18 459 301 645
Cuts at: 3235 4124 5753 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 645 459 301 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3235 1537
Size: 275 1423 4090
Fragments arranged by size:
4090 1423 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2817 3053 3718 1353
Size: 1464 236 665 3423
Fragments arranged by size:
3423 1464 665 236
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4375 4749 4895 387
Size: 1064 138 2786 374 146 1280
Fragments arranged by size:
2786 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2060 2060
Size: 5788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2272 2528 2996 3851 4448 5235
Size: 393 458 182 256 468 855 597 787
Cuts at: 5235 5503 1239
Size: 268 1524
Fragments arranged by size:
1524 855 787 597 468 458 393 268
256 182
EcoO109I rG'GnC_Cy
Cuts at: 2530 2530
Size: 5788
EcoRI G'AATT_C
Cuts at: 3097 3475 3097
Size: 378 5410
FspI TGC'GCA
Cuts at: 4294 4294
Size: 5788
HaeII r_GCGC'y
Cuts at: 1678 2251 4935 1678
Size: 573 2684 2531
Fragments arranged by size:
2684 2531 573
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4429 4461 4503 4535 1373
Size: 32 135 32 2857 32 42 32 2626
Fragments arranged by size:
2857 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3132 3378 669
Size: 988 281 1194 246 3079
Fragments arranged by size:
3079 1194 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5788
HpaI GTT'AAC
Cuts at: 3378 3378
Size: 5788
KpnI G_GTAC'C
Cuts at: 3113 3113
Size: 5788
MfeI C'AATT_G
Cuts at: 3387 3387
Size: 5788
MluI A'CGCG_T
Cuts at: 5664 5664
Size: 5788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4902 5086 1305
Size: 3597 184 2007
Fragments arranged by size:
3597 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2592 10
Size: 54 1425 343 760 3206
Fragments arranged by size:
3206 1425 760 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2438 2697 3746 4105 4264 518
Size: 648 1272 259 1049 359 159 2042
Fragments arranged by size:
2042 1272 1049 648 359 259 159
MspA1I CmG'CkG
Cuts at: 1606 2548 2953 3050 3204 3881 5029 5274 1606
Size: 942 405 97 154 677 1148 245 2120
Fragments arranged by size:
2120 1148 942 677 405 245 154 97
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5236
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5788
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5788
NotI GC'GGCC_GC
Cuts at: 3235 3235
Size: 5788
NspI r_CATG'y
Cuts at: 1132 1229 2504 3146 4691 1132
Size: 97 1275 642 1545 2229
Fragments arranged by size:
2229 1545 1275 642 97
PasI CC'CwG_GG
Cuts at: 1348 3068 1348
Size: 1720 4068
PciI A'CATG_T
Cuts at: 4687 4687
Size: 5788
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2210 2250 1108
Size: 1102 40 4646
Fragments arranged by size:
4646 1102 40
PfoI T'CCnGG_A
Cuts at: 2067 2067
Size: 5788
PmeI GTTT'AAAC
Cuts at: 3092 3092
Size: 5788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2112 2144 3857 3889 5370 5402 2112
Size: 32 1713 32 1481 32 2498
Fragments arranged by size:
2498 1713 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5788
PsiI TTA'TAA
Cuts at: 3358 3358
Size: 5788
PspOMI G'GGCC_C
Cuts at: 2529 2529
Size: 5788
PstI C_TGCA'G
Cuts at: 830 3140 830
Size: 2310 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5788
PvuII CAG'CTG
Cuts at: 2548 2953 3050 2548
Size: 405 97 5286
Fragments arranged by size:
5286 405 97
SacI G_AGCT'C
Cuts at: 2062 2062
Size: 5788
SalI G'TCGA_C
Cuts at: 1936 3130 1936
Size: 1194 4594
SbfI CC_TGCA'GG
Cuts at: 3140 3140
Size: 5788
ScaI AGT'ACT
Cuts at: 4036 5748 4036
Size: 1712 4076
SfcI C'TryA_G
Cuts at: 826 1045 2176 3136 4271 4952 5143 826
Size: 219 1131 960 1135 681 191 1471
Fragments arranged by size:
1471 1135 1131 960 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5788
SmaI CCC'GGG
Cuts at: 1872 2084 3115 1872
Size: 212 1031 4545
Fragments arranged by size:
4545 1031 212
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3892 4793 5055 820
Size: 197 926 1949 901 262 1553
Fragments arranged by size:
1949 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3579 493
Size: 3086 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5788
SphI G_CATG'C
Cuts at: 1132 2504 3146 1132
Size: 1372 642 3774
Fragments arranged by size:
3774 1372 642
SspI AAT'ATT
Cuts at: 5 52 3712 5
Size: 47 3660 2081
Fragments arranged by size:
3660 2081 47
StuI AGG'CCT
Cuts at: 1517 2933 1517
Size: 1416 4372
StyI C'CwwG_G
Cuts at: 513 1065 2194 2452 2686 3227 513
Size: 552 1129 258 234 541 3074
Fragments arranged by size:
3074 1129 552 541 258 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2481 3026 3815 3832 3985 4170 4615
Size: 1255 901 545 789 17 153 185 445
Cuts at: 4615 325
Size: 1498
Fragments arranged by size:
1498 1255 901 789 545 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2148 2971 4034
Size: 274 80 33 51 1443 171 823 1063
Cuts at: 4034 5746 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1063 823 274 171 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2038 2070 5748 5780 2038
Size: 32 3678 32 2046
Fragments arranged by size:
3678 2046 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2665 1316
Size: 1349 4439
XbaI T'CTAG_A
Cuts at: 3124 3124
Size: 5788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2269 2530 2269
Size: 261 5527
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5788
XmaI C'CCGG_G
Cuts at: 1870 2082 3113 1870
Size: 212 1031 4545
Fragments arranged by size:
4545 1031 212
XmnI GAAnn'nnTTC
Cuts at: 3917 3917
Size: 5788
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3596 276
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI