Restriction map for
pFN21AE0805
(Circular) (Six-base) MAPSORT of: pFN21AE0805.seq Check: 4848 from: 1 to: 5785
pFN21AE0805 5785 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2800 2924 2800
Size: 124 5661
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3595 278
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2535 2658 3128 1937
Size: 598 123 470 4594
Fragments arranged by size:
4594 598 470 123
Acc65I G'GTAC_C
Cuts at: 3106 3106
Size: 5785
AclI AA'CG_TT
Cuts at: 1563 3912 4285 1563
Size: 2349 373 3063
Fragments arranged by size:
3063 2349 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2176 3848 5232 5500 1632
Size: 544 1672 1384 268 1917
Fragments arranged by size:
1917 1672 1384 544 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5588
AflIII A'CryG_T
Cuts at: 1203 2742 4684 5661 1203
Size: 1539 1942 977 1327
Fragments arranged by size:
1942 1539 1327 977
AgeI A'CCGG_T
Cuts at: 4616 4616
Size: 5785
AhdI GACnn_n'nnGTC
Cuts at: 4514 4514
Size: 5785
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5785
AlwNI CAG_nnn'CTG
Cuts at: 3528 5100 3528
Size: 1572 4213
ApaI G_GGCC'C
Cuts at: 2884 2884
Size: 5785
ApaLI G'TGCA_C
Cuts at: 2738 3842 4998 2738
Size: 1104 1156 3525
Fragments arranged by size:
3525 1156 1104
ApoI r'AATT_y
Cuts at: 1493 3094 3320 3472 5581 1493
Size: 1601 226 152 2109 1697
Fragments arranged by size:
2109 1697 1601 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4339 160
Size: 872 3307 1606
Fragments arranged by size:
3307 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
AvaI C'yCGr_G
Cuts at: 1870 1943 2240 3110 1870
Size: 73 297 870 4545
Fragments arranged by size:
4545 870 297 73
BamHI G'GATC_C
Cuts at: 3115 3482 3115
Size: 367 5418
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2726 3106 4561 618
Size: 325 217 627 939 380 1455 1842
Fragments arranged by size:
1842 1455 939 627 380 325 217
BanII G_rGCy'C
Cuts at: 1391 2088 2884 3104 1391
Size: 697 796 220 4072
Fragments arranged by size:
4072 796 697 220
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2221 928
Size: 971 322 4492
Fragments arranged by size:
4492 971 322
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3976 4010 1903
Size: 34 2039 34 3678
Fragments arranged by size:
3678 2039 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3677 4887 3677
Size: 1210 4575
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5785
BfrBI ATG'CAT
Cuts at: 2826 2826
Size: 5785
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4396 136
Size: 107 122 71 631 605 2724 1525
Fragments arranged by size:
2724 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5780 5780
Size: 5785
BlpI GC'TnA_GC
Cuts at: 3202 3202
Size: 5785
Bme1580I G_kGCm'C
Cuts at: 1790 2729 2742 2884 3846 5002 1790
Size: 939 13 142 962 1156 2573
Fragments arranged by size:
2573 1156 962 939 142 13
BmgBI CAC'GTC
Cuts at: 1318 2767 1318
Size: 1449 4336
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3516 4474 442
Size: 416 53 83 399 24 2099 958 1753
Fragments arranged by size:
2099 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2048 2102 2414 4445 1239
Size: 809 54 312 2031 2579
Fragments arranged by size:
2579 2031 809 312 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3910 4775 5073 3910
Size: 865 298 4622
Fragments arranged by size:
4622 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2390 4448 882
Size: 617 891 2058 2219
Fragments arranged by size:
2219 2058 891 617
BsaAI yAC'GTr
Cuts at: 493 1204 2737 3576 493
Size: 711 1533 839 2702
Fragments arranged by size:
2702 1533 839 711
BsaBI GATnn'nnATC
Cuts at: 2411 2489 5779 2411
Size: 78 3290 2417
Fragments arranged by size:
3290 2417 78
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2634 3592 3974 4609
Size: 53 83 186 936 1101 958 382 635
Cuts at: 4609 275
Size: 1451
Fragments arranged by size:
1451 1101 958 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3018 4218 4616 4890 5037 5448 3018
Size: 1200 398 274 147 411 3355
Fragments arranged by size:
3355 1200 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2025 2055 2309 2339 1178
Size: 30 165 30 622 30 254 30 4624
Fragments arranged by size:
4624 622 254 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2708 1186
Size: 1522 4263
BseYI C'CCAG_C
Cuts at: 2088 2877 4988 5484 2088
Size: 789 2111 496 2389
Fragments arranged by size:
2389 2111 789 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2306 2747 1163
Size: 573 570 441 4201
Fragments arranged by size:
4201 573 570 441
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3235 3996 5024 1540
Size: 275 186 1234 761 1028 2301
Fragments arranged by size:
2301 1234 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2175 2742 3846 3931 5002 2175
Size: 567 1104 85 1071 2958
Fragments arranged by size:
2958 1104 1071 567 85
BsmI GAATG_Cn'
Cuts at: 2125 3296 3389 2125
Size: 1171 93 4521
Fragments arranged by size:
4521 1171 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2175 2729 2742 2884 3104 3846
Size: 399 298 87 554 13 142 220 742
Cuts at: 3846 3931 5002 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 554 399 298 220 142
87 85 13
BspEI T'CCGG_A
Cuts at: 3018 5448 3018
Size: 2430 3355
BspHI T'CATG_A
Cuts at: 2403 2580 3674 5404 2403
Size: 177 1094 1730 2784
Fragments arranged by size:
2784 1730 1094 177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2215 2328 2800 2924 3140 844
Size: 1371 113 472 124 216 3489
Fragments arranged by size:
3489 1371 472 216 124 113
BsrBI CCG'CTC
Cuts at: 1394 1823 3672 1394
Size: 429 1849 3507
Fragments arranged by size:
3507 1849 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2121 4280 4454 65
Size: 1183 873 2159 174 1396
Fragments arranged by size:
2159 1396 1183 873 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4429 4616 1700
Size: 110 2619 187 2869
Fragments arranged by size:
2869 2619 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5785
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5785
BssSI C'ACGA_G
Cuts at: 3845 4857 3845
Size: 1012 4773
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2120 794
Size: 1326 4459
BstBI TT'CG_AA
Cuts at: 4589 4589
Size: 5785
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5785
BstXI CCAn_nnnn'nTGG
Cuts at: 2392 2857 2392
Size: 465 5320
BstYI r'GATC_y
Cuts at: 1972 3115 3482 3867 3884 5336 5422 5434 5780
Size: 1143 367 385 17 1452 86 12 346
Cuts at: 5780 1972
Size: 1977
Fragments arranged by size:
1977 1452 1143 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2301 513
Size: 552 1236 3997
Fragments arranged by size:
3997 1236 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2512 487
Size: 683 56 1286 3760
Fragments arranged by size:
3760 1286 683 56
BtsI GCAGTG_nn'
Cuts at: 2153 2783 2946 3301 4095 4115 2153
Size: 630 163 355 794 20 3823
Fragments arranged by size:
3823 794 630 355 163 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5785
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5750
DraI TTT'AAA
Cuts at: 3089 3089
Size: 5785
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2543 4792 809
Size: 507 1227 2249 1802
Fragments arranged by size:
2249 1802 1227 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2298 2361 2523
Size: 54 1425 50 275 18 468 63 162
Cuts at: 2523 2613 3232 4121 5750 8
Size: 90 619 889 1629 43
Fragments arranged by size:
1629 1425 889 619 468 275 162 90
63 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3232 1537
Size: 275 1420 4090
Fragments arranged by size:
4090 1420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3715 1353
Size: 2362 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4372 4746 4892 387
Size: 1064 138 2783 374 146 1280
Fragments arranged by size:
2783 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2102 2176 2414 3848 4445 5232
Size: 393 416 54 74 238 1434 597 787
Cuts at: 5232 5500 1239
Size: 268 1524
Fragments arranged by size:
1524 1434 787 597 416 393 268 238
74 54
EcoO109I rG'GnC_Cy
Cuts at: 2596 2881 2596
Size: 285 5500
EcoRI G'AATT_C
Cuts at: 3094 3472 3094
Size: 378 5407
FspI TGC'GCA
Cuts at: 3035 4291 3035
Size: 1256 4529
HaeII r_GCGC'y
Cuts at: 1678 2214 4932 1678
Size: 536 2718 2531
Fragments arranged by size:
2718 2531 536
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2025 2057 4426 4458 4500
Size: 32 135 32 453 32 2369 32 42
Cuts at: 4500 4532 1373
Size: 32 2626
Fragments arranged by size:
2626 2369 453 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2536 3129 3375 669
Size: 988 281 598 593 246 3079
Fragments arranged by size:
3079 988 598 593 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5785
HpaI GTT'AAC
Cuts at: 3375 3375
Size: 5785
KpnI G_GTAC'C
Cuts at: 3110 3110
Size: 5785
MfeI C'AATT_G
Cuts at: 3384 3384
Size: 5785
MluI A'CGCG_T
Cuts at: 5661 5661
Size: 5785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4899 5083 1305
Size: 3594 184 2007
Fragments arranged by size:
3594 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2525 2615 10
Size: 54 1425 343 693 90 3180
Fragments arranged by size:
3180 1425 693 343 90 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2585 2696 3743 4102 4261 518
Size: 648 1419 111 1047 359 159 2042
Fragments arranged by size:
2042 1419 1047 648 359 159 111
MspA1I CmG'CkG
Cuts at: 1606 3201 3878 5026 5271 1606
Size: 1595 677 1148 245 2120
Fragments arranged by size:
2120 1595 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5233
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5785
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5785
NotI GC'GGCC_GC
Cuts at: 3232 3232
Size: 5785
NsiI A_TGCA'T
Cuts at: 2828 2828
Size: 5785
NspI r_CATG'y
Cuts at: 1132 1229 2371 2746 3143 4688 1132
Size: 97 1142 375 397 1545 2229
Fragments arranged by size:
2229 1545 1142 397 375 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5785
PciI A'CATG_T
Cuts at: 2742 4684 2742
Size: 1942 3843
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2461 1108
Size: 1353 4432
PmeI GTTT'AAAC
Cuts at: 3089 3089
Size: 5785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3854 3886 5367 5399 3854
Size: 32 1481 32 4240
Fragments arranged by size:
4240 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5785
PsiI TTA'TAA
Cuts at: 3355 3355
Size: 5785
PspOMI G'GGCC_C
Cuts at: 2880 2880
Size: 5785
PstI C_TGCA'G
Cuts at: 830 2351 3137 830
Size: 1521 786 3478
Fragments arranged by size:
3478 1521 786
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5785
SalI G'TCGA_C
Cuts at: 1936 2534 3127 1936
Size: 598 593 4594
Fragments arranged by size:
4594 598 593
SbfI CC_TGCA'GG
Cuts at: 3137 3137
Size: 5785
ScaI AGT'ACT
Cuts at: 4033 5745 4033
Size: 1712 4073
SexAI A'CCwGG_T
Cuts at: 2898 2967 2898
Size: 69 5716
SfcI C'TryA_G
Cuts at: 826 1045 2347 3133 4268 4949 5140 826
Size: 219 1302 786 1135 681 191 1471
Fragments arranged by size:
1471 1302 1135 786 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5785
SmaI CCC'GGG
Cuts at: 1872 3112 1872
Size: 1240 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3889 4790 5052 820
Size: 197 926 1946 901 262 1553
Fragments arranged by size:
1946 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3576 493
Size: 3083 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5785
SphI G_CATG'C
Cuts at: 1132 3143 1132
Size: 2011 3774
SspI AAT'ATT
Cuts at: 5 52 3709 5
Size: 47 3657 2081
Fragments arranged by size:
3657 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5785
StyI C'CwwG_G
Cuts at: 513 1065 3224 513
Size: 552 2159 3074
Fragments arranged by size:
3074 2159 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3812 3829 3982 4167 4612 325
Size: 1255 2232 17 153 185 445 1498
Fragments arranged by size:
2232 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2334 4031 5743
Size: 274 80 33 51 1443 357 1697 1712
Cuts at: 5743 96
Size: 138
Fragments arranged by size:
1712 1697 1443 357 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5745 5777 5745
Size: 32 5753
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5785
XbaI T'CTAG_A
Cuts at: 3121 3121
Size: 5785
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5785
XmaI C'CCGG_G
Cuts at: 1870 3110 1870
Size: 1240 4545
XmnI GAAnn'nnTTC
Cuts at: 3914 3914
Size: 5785
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3593 276
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI