Restriction map for
pFN21AE0802
(Circular) (Six-base) MAPSORT of: pFN21AE0802.seq Check: 7329 from: 1 to: 5653
pFN21AE0802 5653 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3463 278
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2883 2996 1937
Size: 946 113 4594
Fragments arranged by size:
4594 946 113
Acc65I G'GTAC_C
Cuts at: 2974 2974
Size: 5653
AclI AA'CG_TT
Cuts at: 1563 3780 4153 1563
Size: 2217 373 3063
Fragments arranged by size:
3063 2217 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3716 5100 5368 1632
Size: 2084 1384 268 1917
Fragments arranged by size:
2084 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5456
AflIII A'CryG_T
Cuts at: 1203 2729 2740 4552 5529 1203
Size: 1526 11 1812 977 1327
Fragments arranged by size:
1812 1526 1327 977 11
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 4382 4382
Size: 5653
AleI CACnn'nnGTG
Cuts at: 1166 2745 1166
Size: 1579 4074
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5653
AlwNI CAG_nnn'CTG
Cuts at: 3396 4968 3396
Size: 1572 4081
ApaLI G'TGCA_C
Cuts at: 2792 3710 4866 2792
Size: 918 1156 3579
Fragments arranged by size:
3579 1156 918
ApoI r'AATT_y
Cuts at: 1493 2010 2962 3188 3340 5449 1493
Size: 517 952 226 152 2109 1697
Fragments arranged by size:
2109 1697 952 517 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2978 1870
Size: 73 1035 4545
Fragments arranged by size:
4545 1035 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2174 2207 2174
Size: 33 5620
BamHI G'GATC_C
Cuts at: 2983 3350 2983
Size: 367 5286
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2912 2974 4429 618
Size: 325 217 627 1125 62 1455 1842
Fragments arranged by size:
1842 1455 1125 627 325 217 62
BanII G_rGCy'C
Cuts at: 1391 2670 2972 1391
Size: 1279 302 4072
Fragments arranged by size:
4072 1279 302
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4682
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3844 3878 1903
Size: 34 1907 34 3678
Fragments arranged by size:
3678 1907 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3545 4755 3545
Size: 1210 4443
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5653
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2210 2837 4264
Size: 107 122 71 631 605 538 627 1427
Cuts at: 4264 136
Size: 1525
Fragments arranged by size:
1525 1427 631 627 605 538 122 107
71
BglII A'GATC_T
Cuts at: 5648 5648
Size: 5653
BlpI GC'TnA_GC
Cuts at: 3070 3070
Size: 5653
Bme1580I G_kGCm'C
Cuts at: 1790 2796 3714 4870 1790
Size: 1006 918 1156 2573
Fragments arranged by size:
2573 1156 1006 918
BmgBI CAC'GTC
Cuts at: 1318 2155 2743 1318
Size: 837 588 4228
Fragments arranged by size:
4228 837 588
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3384 4342 442
Size: 416 53 83 399 24 1967 958 1753
Fragments arranged by size:
1967 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2721 4313 1239
Size: 854 628 1592 2579
Fragments arranged by size:
2579 1592 854 628
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3778 4643 4941 3778
Size: 865 298 4490
Fragments arranged by size:
4490 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4316 882
Size: 617 2817 2219
Fragments arranged by size:
2817 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3444 493
Size: 711 2240 2702
Fragments arranged by size:
2702 2240 711
BsaBI GATnn'nnATC
Cuts at: 2117 2654 5647 2117
Size: 537 2993 2123
Fragments arranged by size:
2993 2123 537
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2507 3460 3842 4477
Size: 53 83 186 936 974 953 382 635
Cuts at: 4477 275
Size: 1451
Fragments arranged by size:
1451 974 953 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4086 4484 4758 4905 5316 4086
Size: 398 274 147 411 4423
Fragments arranged by size:
4423 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5428
Fragments arranged by size:
5428 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5653
BseYI C'CCAG_C
Cuts at: 4856 5352 4856
Size: 496 5157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2538 1163
Size: 573 802 4278
Fragments arranged by size:
4278 802 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3103 3864 4892 1540
Size: 275 186 1102 761 1028 2301
Fragments arranged by size:
2301 1102 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2085 2796 3714 3799 4870 2085
Size: 711 918 85 1071 2868
Fragments arranged by size:
2868 1071 918 711 85
BsmI GAATG_Cn'
Cuts at: 2931 3164 3257 2931
Size: 233 93 5327
Fragments arranged by size:
5327 233 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2670 2796 2972 3714 3799 4870
Size: 399 295 585 126 176 742 85 1071
Cuts at: 4870 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 585 399 295 176 126
85
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 3542 5272 3542
Size: 1730 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3008 844
Size: 2164 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3540 1394
Size: 429 1717 3507
Fragments arranged by size:
3507 1717 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4148 4322 65
Size: 1183 2900 174 1396
Fragments arranged by size:
2900 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4297 4484 1700
Size: 110 2487 187 2869
Fragments arranged by size:
2869 2487 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5653
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 3713 4725 3713
Size: 1012 4641
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5653
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5653
BstXI CCAn_nnnn'nTGG
Cuts at: 2372 2372
Size: 5653
BstYI r'GATC_y
Cuts at: 1972 2904 2983 3350 3735 3752 5204 5290 5302
Size: 932 79 367 385 17 1452 86 12
Cuts at: 5302 5648 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 932 385 367 346 86 79
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2908 513
Size: 552 1843 3258
Fragments arranged by size:
3258 1843 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4914
Fragments arranged by size:
4914 683 56
BtsI GCAGTG_nn'
Cuts at: 2424 3169 3963 3983 2424
Size: 745 794 20 4094
Fragments arranged by size:
4094 794 745 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2713 2748 529
Size: 35 2149 35 3434
Fragments arranged by size:
3434 2149 35 35
DraI TTT'AAA
Cuts at: 2957 2957
Size: 5653
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4660 809
Size: 507 3344 1802
Fragments arranged by size:
3344 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2217 2362 2522
Size: 54 1425 50 275 18 387 145 160
Cuts at: 2522 2838 3100 3989 5618 8
Size: 316 262 889 1629 43
Fragments arranged by size:
1629 1425 889 387 316 275 262 160
145 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3100 1537
Size: 275 1288 4090
Fragments arranged by size:
4090 1288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2201 2858 3583 1353
Size: 848 657 725 3423
Fragments arranged by size:
3423 848 725 657
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4240 4614 4760 387
Size: 1064 138 2651 374 146 1280
Fragments arranged by size:
2651 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2721 3716 4313 5100 5368 1239
Size: 393 461 628 995 597 787 268 1524
Fragments arranged by size:
1524 995 787 628 597 461 393 268
EcoRI G'AATT_C
Cuts at: 2962 3340 2962
Size: 378 5275
EcoRV GAT'ATC
Cuts at: 2094 2094
Size: 5653
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2321 2321
Size: 5653
FspI TGC'GCA
Cuts at: 4159 4159
Size: 5653
HaeII r_GCGC'y
Cuts at: 1678 4800 1678
Size: 3122 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2440 2472 4294 4326 4368
Size: 32 135 32 868 32 1822 32 42
Cuts at: 4368 4400 1373
Size: 32 2626
Fragments arranged by size:
2626 1822 868 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2593 2997 3243 669
Size: 988 281 655 404 246 3079
Fragments arranged by size:
3079 988 655 404 281 246
HindIII A'AGCT_T
Cuts at: 748 2298 748
Size: 1550 4103
HpaI GTT'AAC
Cuts at: 2593 3243 2593
Size: 650 5003
KpnI G_GTAC'C
Cuts at: 2978 2978
Size: 5653
MfeI C'AATT_G
Cuts at: 3252 3252
Size: 5653
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4767 4951 1305
Size: 3462 184 2007
Fragments arranged by size:
3462 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2364 2524 2840 10
Size: 54 1425 343 532 160 316 2823
Fragments arranged by size:
2823 1425 532 343 316 160 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2745 3611 3970 4129 518
Size: 648 1579 866 359 159 2042
Fragments arranged by size:
2042 1579 866 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2352 3069 3746 4894 5139 1606
Size: 746 717 677 1148 245 2120
Fragments arranged by size:
2120 1148 746 717 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2908 513
Size: 552 1843 3258
Fragments arranged by size:
3258 1843 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 2733 3011 4556 1132
Size: 97 1504 278 1545 2229
Fragments arranged by size:
2229 1545 1504 278 97
PasI CC'CwG_GG
Cuts at: 1348 2066 2431 1348
Size: 718 365 4570
Fragments arranged by size:
4570 718 365
PciI A'CATG_T
Cuts at: 2729 4552 2729
Size: 1823 3830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5653
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 5235 5267 3722
Size: 32 1481 32 4108
Fragments arranged by size:
4108 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 3223 3223
Size: 5653
PstI C_TGCA'G
Cuts at: 830 3005 830
Size: 2175 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
PvuII CAG'CTG
Cuts at: 2352 2352
Size: 5653
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SbfI CC_TGCA'GG
Cuts at: 3005 3005
Size: 5653
ScaI AGT'ACT
Cuts at: 3901 5613 3901
Size: 1712 3941
SfcI C'TryA_G
Cuts at: 826 1045 2250 2393 2787 2884 3001 4136 4817
Size: 219 1205 143 394 97 117 1135 681
Cuts at: 4817 5008 826
Size: 191 1471
Fragments arranged by size:
1471 1205 1135 681 394 219 191 143
117 97
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2980 1872
Size: 1108 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3757 4658 4920 820
Size: 197 926 1814 901 262 1553
Fragments arranged by size:
1814 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5653
SphI G_CATG'C
Cuts at: 1132 3011 1132
Size: 1879 3774
SspI AAT'ATT
Cuts at: 5 52 3577 5
Size: 47 3525 2081
Fragments arranged by size:
3525 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5653
StyI C'CwwG_G
Cuts at: 513 1065 2131 2800 2898 2908 3092 513
Size: 552 1066 669 98 10 184 3074
Fragments arranged by size:
3074 1066 669 552 184 98 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3680 3697 3850 4035 4480 325
Size: 1255 2100 17 153 185 445 1498
Fragments arranged by size:
2100 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3899 5611 96
Size: 274 80 33 51 1443 1922 1712 138
Fragments arranged by size:
1922 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2796 2828 5613 5645 2796
Size: 32 2785 32 2804
Fragments arranged by size:
2804 2785 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5653
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2212 2212
Size: 5653
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5653
XmaI C'CCGG_G
Cuts at: 1870 2978 1870
Size: 1108 4545
XmnI GAAnn'nnTTC
Cuts at: 3782 3782
Size: 5653
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3461 276
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI