Restriction map for
pFN21AE0796
(Circular) (Six-base) MAPSORT of: pFN21AE0796.seq Check: 3797 from: 1 to: 5977
pFN21AE0796 5977 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3787 278
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3320 1937
Size: 1383 4594
Acc65I G'GTAC_C
Cuts at: 3298 3298
Size: 5977
AclI AA'CG_TT
Cuts at: 1563 4104 4477 1563
Size: 2541 373 3063
Fragments arranged by size:
3063 2541 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4040 5424 5692 1632
Size: 2408 1384 268 1917
Fragments arranged by size:
2408 1917 1384 268
AfeI AGC'GCT
Cuts at: 2723 2723
Size: 5977
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5780
AflIII A'CryG_T
Cuts at: 1203 4876 5853 1203
Size: 3673 977 1327
Fragments arranged by size:
3673 1327 977
AgeI A'CCGG_T
Cuts at: 4808 4808
Size: 5977
AhdI GACnn_n'nnGTC
Cuts at: 4706 4706
Size: 5977
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5977
AlwNI CAG_nnn'CTG
Cuts at: 3720 5292 3720
Size: 1572 4405
ApaI G_GGCC'C
Cuts at: 3229 3229
Size: 5977
ApaLI G'TGCA_C
Cuts at: 4034 5190 4034
Size: 1156 4821
ApoI r'AATT_y
Cuts at: 1493 3286 3512 3664 5773 1493
Size: 1793 226 152 2109 1697
Fragments arranged by size:
2109 1793 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4531 160
Size: 872 3499 1606
Fragments arranged by size:
3499 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
AvaI C'yCGr_G
Cuts at: 1870 1943 3302 1870
Size: 73 1359 4545
Fragments arranged by size:
4545 1359 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2599 2632 2599
Size: 33 5944
BamHI G'GATC_C
Cuts at: 3307 3674 3307
Size: 367 5610
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2198 3298 4753 618
Size: 325 217 627 411 1100 1455 1842
Fragments arranged by size:
1842 1455 1100 627 411 325 217
BanII G_rGCy'C
Cuts at: 1391 3229 3263 3296 1391
Size: 1838 34 33 4072
Fragments arranged by size:
4072 1838 34 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2683 3258 928
Size: 971 784 575 3647
Fragments arranged by size:
3647 971 784 575
BbvCI CC'TCA_GC
Cuts at: 3090 3090
Size: 5977
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4168 4202 1903
Size: 34 2231 34 3678
Fragments arranged by size:
3678 2231 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3869 5079 3869
Size: 1210 4767
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5977
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4588 136
Size: 107 122 71 631 605 2916 1525
Fragments arranged by size:
2916 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5972 5972
Size: 5977
BlpI GC'TnA_GC
Cuts at: 3394 3394
Size: 5977
Bme1580I G_kGCm'C
Cuts at: 1790 2262 2905 3229 4038 5194 1790
Size: 472 643 324 809 1156 2573
Fragments arranged by size:
2573 1156 809 643 472 324
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5977
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2908 3708 4666
Size: 416 53 83 399 24 1491 800 958
Cuts at: 4666 442
Size: 1753
Fragments arranged by size:
1753 1491 958 800 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2529 2544 2896 3000 3032 4637 1239
Size: 1290 15 352 104 32 1605 2579
Fragments arranged by size:
2579 1605 1290 352 104 32 15
Bpu10I CC'TnA_GC
Cuts at: 3090 3090
Size: 5977
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4102 4967 5265 4102
Size: 865 298 4814
Fragments arranged by size:
4814 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4640 882
Size: 617 3141 2219
Fragments arranged by size:
3141 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3768 493
Size: 711 2564 2702
Fragments arranged by size:
2702 2564 711
BsaBI GATnn'nnATC
Cuts at: 5971 5971
Size: 5977
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3784 4166 4801 275
Size: 53 83 186 936 2251 382 635 1451
Fragments arranged by size:
2251 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4410 4808 5082 5229 5640 4410
Size: 398 274 147 411 4747
Fragments arranged by size:
4747 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2282 2312 2918 2948 1178
Size: 30 165 30 879 30 606 30 4207
Fragments arranged by size:
4207 879 606 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2098 3161 1186
Size: 912 1063 4002
Fragments arranged by size:
4002 1063 912
BseYI C'CCAG_C
Cuts at: 2811 5180 5676 2811
Size: 2369 496 3112
Fragments arranged by size:
3112 2369 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3023 1163
Size: 573 1287 4117
Fragments arranged by size:
4117 1287 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3427 4188 5216 1540
Size: 275 186 1426 761 1028 2301
Fragments arranged by size:
2301 1426 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4038 4123 5194 4038
Size: 85 1071 4821
Fragments arranged by size:
4821 1071 85
BsmI GAATG_Cn'
Cuts at: 3488 3581 3488
Size: 93 5884
BsmBI CGTCTCn'nnnn_
Cuts at: 2285 3214 2285
Size: 929 5048
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2262 2905 3229 3263 3296 4038 4123
Size: 399 472 643 324 34 33 742 85
Cuts at: 4123 5194 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 643 472 399 324 85
34 33
BspEI T'CCGG_A
Cuts at: 5640 5640
Size: 5977
BspHI T'CATG_A
Cuts at: 3866 5596 3866
Size: 1730 4247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3332 844
Size: 2488 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3864 1394
Size: 429 2041 3507
Fragments arranged by size:
3507 2041 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2499 4472 4646 65
Size: 1183 1251 1973 174 1396
Fragments arranged by size:
1973 1396 1251 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4621 4808 1700
Size: 110 2811 187 2869
Fragments arranged by size:
2869 2811 187 110
BsrGI T'GTAC_A
Cuts at: 96 2145 96
Size: 2049 3928
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5977
BssSI C'ACGA_G
Cuts at: 4037 5049 4037
Size: 1012 4965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2838 794
Size: 2044 3933
BstBI TT'CG_AA
Cuts at: 4781 4781
Size: 5977
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5977
BstXI CCAn_nnnn'nTGG
Cuts at: 2452 2944 2452
Size: 492 5485
BstYI r'GATC_y
Cuts at: 1972 3307 3674 4059 4076 5528 5614 5626 5972
Size: 1335 367 385 17 1452 86 12 346
Cuts at: 5972 1972
Size: 1977
Fragments arranged by size:
1977 1452 1335 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2676 2676
Size: 5977
BtgI C'CryG_G
Cuts at: 513 1065 2004 2996 3244 513
Size: 552 939 992 248 3246
Fragments arranged by size:
3246 992 939 552 248
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2050 3082 487
Size: 683 56 824 1032 3382
Fragments arranged by size:
3382 1032 824 683 56
BtsI GCAGTG_nn'
Cuts at: 2596 2791 3493 4287 4307 2596
Size: 195 702 794 20 4266
Fragments arranged by size:
4266 794 702 195 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5942
DraI TTT'AAA
Cuts at: 3281 3281
Size: 5977
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4984 809
Size: 507 3668 1802
Fragments arranged by size:
3668 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3424 4313 5942
Size: 54 1425 50 275 18 1594 889 1629
Cuts at: 5942 8
Size: 43
Fragments arranged by size:
1629 1594 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3424 1537
Size: 275 1612 4090
Fragments arranged by size:
4090 1612 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3086 3123 3907 1353
Size: 1733 37 784 3423
Fragments arranged by size:
3423 1733 784 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4564 4938 5084 387
Size: 1064 138 2975 374 146 1280
Fragments arranged by size:
2975 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2529 2544 2896 3000 3032 4040 4637
Size: 393 897 15 352 104 32 1008 597
Cuts at: 4637 5424 5692 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1008 897 787 597 393 352 268
104 32 15
EcoNI CCTnn'n_nnAGG
Cuts at: 3233 3233
Size: 5977
EcoO109I rG'GnC_Cy
Cuts at: 3226 3226
Size: 5977
EcoRI G'AATT_C
Cuts at: 3286 3664 3286
Size: 378 5599
FspI TGC'GCA
Cuts at: 4483 4483
Size: 5977
HaeII r_GCGC'y
Cuts at: 1678 2725 2976 5124 1678
Size: 1047 251 2148 2531
Fragments arranged by size:
2531 2148 1047 251
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2284 2316 2382 2414 2918
Size: 32 135 32 712 32 66 32 504
Cuts at: 2918 2950 4618 4650 4692 4724 1373
Size: 32 1668 32 42 32 2626
Fragments arranged by size:
2626 1668 712 504 135 66 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3321 3567 669
Size: 988 281 1383 246 3079
Fragments arranged by size:
3079 1383 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3185 748
Size: 2437 3540
HpaI GTT'AAC
Cuts at: 3567 3567
Size: 5977
KpnI G_GTAC'C
Cuts at: 3302 3302
Size: 5977
MfeI C'AATT_G
Cuts at: 3576 3576
Size: 5977
MluI A'CGCG_T
Cuts at: 5853 5853
Size: 5977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2004 2246 5091 5275 1305
Size: 699 242 2845 184 2007
Fragments arranged by size:
2845 2007 699 242 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4155
Fragments arranged by size:
4155 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2430 3935 4294 4453 518
Size: 648 1264 1505 359 159 2042
Fragments arranged by size:
2042 1505 1264 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3089 3393 4070 5218 5463 1606
Size: 1483 304 677 1148 245 2120
Fragments arranged by size:
2120 1483 1148 677 304 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2996 513
Size: 552 939 992 3494
Fragments arranged by size:
3494 992 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5977
NotI GC'GGCC_GC
Cuts at: 3424 3424
Size: 5977
NspI r_CATG'y
Cuts at: 1132 1229 3335 4880 1132
Size: 97 2106 1545 2229
Fragments arranged by size:
2229 2106 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5977
PciI A'CATG_T
Cuts at: 4876 4876
Size: 5977
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5977
PfoI T'CCnGG_A
Cuts at: 2865 2865
Size: 5977
PmeI GTTT'AAAC
Cuts at: 3281 3281
Size: 5977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4046 4078 5559 5591 4046
Size: 32 1481 32 4432
Fragments arranged by size:
4432 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5977
PsiI TTA'TAA
Cuts at: 3547 3547
Size: 5977
PspOMI G'GGCC_C
Cuts at: 3225 3225
Size: 5977
PstI C_TGCA'G
Cuts at: 830 2662 2834 2991 3329 830
Size: 1832 172 157 338 3478
Fragments arranged by size:
3478 1832 338 172 157
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5977
PvuII CAG'CTG
Cuts at: 3089 3089
Size: 5977
SalI G'TCGA_C
Cuts at: 1936 3319 1936
Size: 1383 4594
SapI GCTCTTCn'nnn_
Cuts at: 3086 3086
Size: 5977
SbfI CC_TGCA'GG
Cuts at: 3329 3329
Size: 5977
ScaI AGT'ACT
Cuts at: 4225 5937 4225
Size: 1712 4265
SfcI C'TryA_G
Cuts at: 826 1045 2034 2210 2658 2830 2987 3325 4460
Size: 219 989 176 448 172 157 338 1135
Cuts at: 4460 5141 5332 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 989 681 448 338 219 191
176 172 157
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5977
SmaI CCC'GGG
Cuts at: 1872 3304 1872
Size: 1432 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4081 4982 5244 820
Size: 197 926 2138 901 262 1553
Fragments arranged by size:
2138 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3768 493
Size: 3275 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5977
SphI G_CATG'C
Cuts at: 1132 3335 1132
Size: 2203 3774
SspI AAT'ATT
Cuts at: 5 52 3901 5
Size: 47 3849 2081
Fragments arranged by size:
3849 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5977
StyI C'CwwG_G
Cuts at: 513 1065 2004 2996 3416 513
Size: 552 939 992 420 3074
Fragments arranged by size:
3074 992 939 552 420
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2989 3009 4004 4021 4174 4359 4804
Size: 1255 1409 20 995 17 153 185 445
Cuts at: 4804 325
Size: 1498
Fragments arranged by size:
1498 1409 1255 995 445 185 153 20
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2145 4223 5935
Size: 274 80 33 51 1443 168 2078 1712
Cuts at: 5935 96
Size: 138
Fragments arranged by size:
2078 1712 1443 274 168 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5937 5969 5937
Size: 32 5945
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5977
XbaI T'CTAG_A
Cuts at: 3313 3313
Size: 5977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3242 3242
Size: 5977
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5977
XmaI C'CCGG_G
Cuts at: 1870 3302 1870
Size: 1432 4545
XmnI GAAnn'nnTTC
Cuts at: 2058 4106 2058
Size: 2048 3929
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3785 276
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbeI BfrBI BplI BsiWI
BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI