Restriction map for
pFN21AE0795
(Circular) (Six-base) MAPSORT of: pFN21AE0795.seq Check: 3339 from: 1 to: 5779
pFN21AE0795 5779 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2094 2215 2094
Size: 121 5658
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3589 278
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2277 2942 3122 1937
Size: 340 665 180 4594
Fragments arranged by size:
4594 665 340 180
Acc65I G'GTAC_C
Cuts at: 3100 3100
Size: 5779
AclI AA'CG_TT
Cuts at: 1563 3906 4279 1563
Size: 2343 373 3063
Fragments arranged by size:
3063 2343 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2559 3052 3842 5226 5494 1632
Size: 927 493 790 1384 268 1917
Fragments arranged by size:
1917 1384 927 790 493 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5582
AflIII A'CryG_T
Cuts at: 1203 4678 5655 1203
Size: 3475 977 1327
Fragments arranged by size:
3475 1327 977
AgeI A'CCGG_T
Cuts at: 4610 4610
Size: 5779
AhdI GACnn_n'nnGTC
Cuts at: 2650 4508 2650
Size: 1858 3921
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5779
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5779
AlwNI CAG_nnn'CTG
Cuts at: 3522 5094 3522
Size: 1572 4207
ApaI G_GGCC'C
Cuts at: 2486 2590 3007 3034 2486
Size: 104 417 27 5231
Fragments arranged by size:
5231 417 104 27
ApaLI G'TGCA_C
Cuts at: 3836 4992 3836
Size: 1156 4623
ApoI r'AATT_y
Cuts at: 1493 2896 3088 3314 3466 5575 1493
Size: 1403 192 226 152 2109 1697
Fragments arranged by size:
2109 1697 1403 226 192 152
AseI AT'TA_AT
Cuts at: 160 1032 4333 160
Size: 872 3301 1606
Fragments arranged by size:
3301 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
AvaI C'yCGr_G
Cuts at: 1870 1943 2282 3104 1870
Size: 73 339 822 4545
Fragments arranged by size:
4545 822 339 73
BamHI G'GATC_C
Cuts at: 3109 3476 3109
Size: 367 5412
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 3100 4555 618
Size: 325 217 627 242 1071 1455 1842
Fragments arranged by size:
1842 1455 1071 627 325 242 217
BanII G_rGCy'C
Cuts at: 1391 2136 2486 2583 2590 2846 3007 3034 3098
Size: 745 350 97 7 256 161 27 64
Cuts at: 3098 1391
Size: 4072
Fragments arranged by size:
4072 745 350 256 161 97 64 27
7
BbeI G_GCGC'C
Cuts at: 2033 2033
Size: 5779
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4808
BbvCI CC'TCA_GC
Cuts at: 2568 2568
Size: 5779
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3970 4004 1903
Size: 34 2033 34 3678
Fragments arranged by size:
3678 2033 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3671 4881 3671
Size: 1210 4569
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5779
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2153 2490 2663
Size: 107 122 71 631 605 481 337 173
Cuts at: 2663 4390 136
Size: 1727 1525
Fragments arranged by size:
1727 1525 631 605 481 337 173 122
107 71
BglII A'GATC_T
Cuts at: 5774 5774
Size: 5779
BlpI GC'TnA_GC
Cuts at: 3196 3196
Size: 5779
Bme1580I G_kGCm'C
Cuts at: 1790 2486 2590 2778 3007 3034 3840 4996 1790
Size: 696 104 188 229 27 806 1156 2573
Fragments arranged by size:
2573 1156 806 696 229 188 104 27
BmgBI CAC'GTC
Cuts at: 1318 2647 1318
Size: 1329 4450
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2481 3510 4468
Size: 416 53 83 399 24 1064 1029 958
Cuts at: 4468 442
Size: 1753
Fragments arranged by size:
1753 1064 1029 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5779
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 5779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2857 3067 4439 1239
Size: 836 782 210 1372 2579
Fragments arranged by size:
2579 1372 836 782 210
Bpu10I CC'TnA_GC
Cuts at: 2568 2568
Size: 5779
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3904 4769 5067 3904
Size: 865 298 4616
Fragments arranged by size:
4616 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2207 2672 4442 882
Size: 617 708 465 1770 2219
Fragments arranged by size:
2219 1770 708 617 465
BsaAI yAC'GTr
Cuts at: 493 1204 3570 493
Size: 711 2366 2702
Fragments arranged by size:
2702 2366 711
BsaBI GATnn'nnATC
Cuts at: 5773 5773
Size: 5779
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2030 2313 3586 3968
Size: 53 83 186 936 497 283 1273 382
Cuts at: 3968 4603 275
Size: 635 1451
Fragments arranged by size:
1451 1273 936 635 497 382 283 186
83 53
BsaWI w'CCGG_w
Cuts at: 2541 4212 4610 4884 5031 5442 2541
Size: 1671 398 274 147 411 2878
Fragments arranged by size:
2878 1671 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2543 2573 1178
Size: 30 165 30 1140 30 4384
Fragments arranged by size:
4384 1140 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2342 2903 2938 2941 1186
Size: 1156 561 35 3 4024
Fragments arranged by size:
4024 1156 561 35 3
BseYI C'CCAG_C
Cuts at: 4982 5478 4982
Size: 496 5283
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2072 2408 2492 1163
Size: 573 336 336 84 4450
Fragments arranged by size:
4450 573 336 336 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2025 2148 2376 3229 3990 5018
Size: 275 186 24 123 228 853 761 1028
Cuts at: 5018 1540
Size: 2301
Fragments arranged by size:
2301 1028 853 761 275 228 186 123
24
BsiHKAI G_wGCw'C
Cuts at: 2754 3840 3925 4996 2754
Size: 1086 85 1071 3537
Fragments arranged by size:
3537 1086 1071 85
BsmI GAATG_Cn'
Cuts at: 3290 3383 3290
Size: 93 5686
BsmBI CGTCTCn'nnnn_
Cuts at: 2279 2279
Size: 5779
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2486 2583 2590 2754 2778 2846
Size: 399 346 350 97 7 164 24 68
Cuts at: 2846 3007 3034 3098 3840 3925 4996 1391
Size: 161 27 64 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 350 346 164 161
97 85 68 64 27 24 7
BspEI T'CCGG_A
Cuts at: 5442 5442
Size: 5779
BspHI T'CATG_A
Cuts at: 3668 5398 3668
Size: 1730 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2094 2215 2514 3134 844
Size: 1250 121 299 620 3489
Fragments arranged by size:
3489 1250 620 299 121
BsrBI CCG'CTC
Cuts at: 1394 1823 3666 1394
Size: 429 1843 3507
Fragments arranged by size:
3507 1843 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4274 4448 65
Size: 1183 3026 174 1396
Fragments arranged by size:
3026 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2143 2175 2371 4423 4610 1700
Size: 110 333 32 196 2052 187 2869
Fragments arranged by size:
2869 2052 333 196 187 110 32
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5779
BssHII G'CGCG_C
Cuts at: 1925 2338 1925
Size: 413 5366
BssSI C'ACGA_G
Cuts at: 3839 4851 3839
Size: 1012 4767
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5779
BstBI TT'CG_AA
Cuts at: 4583 4583
Size: 5779
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5779
BstXI CCAn_nnnn'nTGG
Cuts at: 2689 2689
Size: 5779
BstYI r'GATC_y
Cuts at: 1972 2864 3109 3476 3861 3878 5330 5416 5428
Size: 892 245 367 385 17 1452 86 12
Cuts at: 5428 5774 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 892 385 367 346 245 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2988 513
Size: 552 1923 3304
Fragments arranged by size:
3304 1923 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5040
Fragments arranged by size:
5040 683 56
BtsI GCAGTG_nn'
Cuts at: 3295 4089 4109 3295
Size: 794 20 4965
Fragments arranged by size:
4965 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5779
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5744
DraI TTT'AAA
Cuts at: 3083 3083
Size: 5779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4786 809
Size: 507 3470 1802
Fragments arranged by size:
3470 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2145 2373
Size: 54 1425 50 275 18 192 123 228
Cuts at: 2373 2985 2991 3226 4115 5744 8
Size: 612 6 235 889 1629 43
Fragments arranged by size:
1629 1425 889 612 275 235 228 192
123 54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2022 2145 2373 3226 1537
Size: 275 210 123 228 853 4090
Fragments arranged by size:
4090 853 275 228 210 123
EarI CTCTTCn'nnn_
Cuts at: 1353 3071 3709 1353
Size: 1718 638 3423
Fragments arranged by size:
3423 1718 638
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2644 2857 4366 4740 4886 387
Size: 1064 138 1055 213 1509 374 146 1280
Fragments arranged by size:
1509 1280 1064 1055 374 213 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2559 2857 3052 3067 3842 4439
Size: 393 443 484 298 195 15 775 597
Cuts at: 4439 5226 5494 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 775 597 484 443 393 298
268 195 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2057 2825 2057
Size: 768 5011
EcoO109I rG'GnC_Cy
Cuts at: 2482 2483 3003 3031 2482
Size: 1 520 28 5230
Fragments arranged by size:
5230 520 28 1
EcoRI G'AATT_C
Cuts at: 2896 3088 3466 2896
Size: 192 378 5209
Fragments arranged by size:
5209 378 192
FspI TGC'GCA
Cuts at: 4285 4285
Size: 5779
HaeII r_GCGC'y
Cuts at: 1678 2033 2354 2936 4926 1678
Size: 355 321 582 1990 2531
Fragments arranged by size:
2531 1990 582 355 321
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2543 2575 2636 2668 2728
Size: 32 135 32 971 32 61 32 60
Cuts at: 2728 2760 4420 4452 4494 4526 1373
Size: 32 1660 32 42 32 2626
Fragments arranged by size:
2626 1660 971 135 61 60 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2278 3123 3369 669
Size: 988 281 340 845 246 3079
Fragments arranged by size:
3079 988 845 340 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5779
HpaI GTT'AAC
Cuts at: 3369 3369
Size: 5779
KasI G'GCGC_C
Cuts at: 2029 2029
Size: 5779
KpnI G_GTAC'C
Cuts at: 3104 3104
Size: 5779
MfeI C'AATT_G
Cuts at: 3378 3378
Size: 5779
MluI A'CGCG_T
Cuts at: 5655 5655
Size: 5779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2979 4893 5077 1305
Size: 1674 1914 184 2007
Fragments arranged by size:
2007 1914 1674 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2987 2993 10
Size: 54 1425 343 1155 6 2796
Fragments arranged by size:
2796 1425 1155 343 54 6
MslI CAynn'nnrTG
Cuts at: 518 1166 2757 3737 4096 4255 518
Size: 648 1591 980 359 159 2042
Fragments arranged by size:
2042 1591 980 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2021 2299 3195 3872 5020 5265 1606
Size: 415 278 896 677 1148 245 2120
Fragments arranged by size:
2120 1148 896 677 415 278 245
NaeI GCC'GGC
Cuts at: 2145 2373 2145
Size: 228 5551
NarI GG'CG_CC
Cuts at: 2030 2030
Size: 5779
NcoI C'CATG_G
Cuts at: 513 1065 2988 513
Size: 552 1923 3304
Fragments arranged by size:
3304 1923 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5779
NgoMIV G'CCGG_C
Cuts at: 2143 2371 2143
Size: 228 5551
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5779
NotI GC'GGCC_GC
Cuts at: 3226 3226
Size: 5779
NruI TCG'CGA
Cuts at: 2418 2418
Size: 5779
NspI r_CATG'y
Cuts at: 1132 1229 3137 4682 1132
Size: 97 1908 1545 2229
Fragments arranged by size:
2229 1908 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5779
PciI A'CATG_T
Cuts at: 4678 4678
Size: 5779
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2227 1108
Size: 1119 4660
PmeI GTTT'AAAC
Cuts at: 3083 3083
Size: 5779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3848 3880 5361 5393 3848
Size: 32 1481 32 4234
Fragments arranged by size:
4234 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5779
PsiI TTA'TAA
Cuts at: 3349 3349
Size: 5779
PspOMI G'GGCC_C
Cuts at: 2482 2586 3003 3030 2482
Size: 104 417 27 5231
Fragments arranged by size:
5231 417 104 27
PstI C_TGCA'G
Cuts at: 830 2304 3131 830
Size: 1474 827 3478
Fragments arranged by size:
3478 1474 827
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5779
PvuII CAG'CTG
Cuts at: 2299 2299
Size: 5779
SalI G'TCGA_C
Cuts at: 1936 2276 3121 1936
Size: 340 845 4594
Fragments arranged by size:
4594 845 340
SbfI CC_TGCA'GG
Cuts at: 3131 3131
Size: 5779
ScaI AGT'ACT
Cuts at: 4027 5739 4027
Size: 1712 4067
SfcI C'TryA_G
Cuts at: 826 1045 2300 3127 4262 4943 5134 826
Size: 219 1255 827 1135 681 191 1471
Fragments arranged by size:
1471 1255 1135 827 681 219 191
SfoI GGC'GCC
Cuts at: 2031 2031
Size: 5779
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5779
SmaI CCC'GGG
Cuts at: 1872 3106 1872
Size: 1234 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3883 4784 5046 820
Size: 197 926 1940 901 262 1553
Fragments arranged by size:
1940 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3570 493
Size: 3077 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5779
SphI G_CATG'C
Cuts at: 1132 3137 1132
Size: 2005 3774
SspI AAT'ATT
Cuts at: 5 52 3703 5
Size: 47 3651 2081
Fragments arranged by size:
3651 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5779
StyI C'CwwG_G
Cuts at: 513 1065 2835 2988 3218 513
Size: 552 1770 153 230 3074
Fragments arranged by size:
3074 1770 552 230 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3806 3823 3976 4161 4606 325
Size: 1255 2226 17 153 185 445 1498
Fragments arranged by size:
2226 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2115 4025 5737
Size: 274 80 33 51 1443 138 1910 1712
Cuts at: 5737 96
Size: 138
Fragments arranged by size:
1910 1712 1443 274 138 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5739 5771 5739
Size: 32 5747
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5779
XbaI T'CTAG_A
Cuts at: 3115 3115
Size: 5779
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2689 2854 2689
Size: 165 5614
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5779
XmaI C'CCGG_G
Cuts at: 1870 3104 1870
Size: 1234 4545
XmnI GAAnn'nnTTC
Cuts at: 3908 3908
Size: 5779
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3587 276
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI