Restriction map for
pFN21AE0791
(Circular) (Six-base) MAPSORT of: pFN21AE0791.seq Check: 4213 from: 1 to: 5776
pFN21AE0791 5776 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3119 1937
Size: 1182 4594
Acc65I G'GTAC_C
Cuts at: 3097 3097
Size: 5776
AclI AA'CG_TT
Cuts at: 1563 3903 4276 1563
Size: 2340 373 3063
Fragments arranged by size:
3063 2340 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2308 2410 3839 5223 5491 1632
Size: 676 102 1429 1384 268 1917
Fragments arranged by size:
1917 1429 1384 676 268 102
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 2827 4675 5652 1203
Size: 1624 1848 977 1327
Fragments arranged by size:
1848 1624 1327 977
AgeI A'CCGG_T
Cuts at: 4607 4607
Size: 5776
AhdI GACnn_n'nnGTC
Cuts at: 4505 4505
Size: 5776
AleI CACnn'nnGTG
Cuts at: 1166 2703 1166
Size: 1537 4239
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5776
AlwNI CAG_nnn'CTG
Cuts at: 3519 5091 3519
Size: 1572 4204
ApaLI G'TGCA_C
Cuts at: 3833 4989 3833
Size: 1156 4620
ApoI r'AATT_y
Cuts at: 1493 2539 2732 3085 3311 3463 5572 1493
Size: 1046 193 353 226 152 2109 1697
Fragments arranged by size:
2109 1697 1046 353 226 193 152
AseI AT'TA_AT
Cuts at: 160 1032 3056 4330 160
Size: 872 2024 1274 1606
Fragments arranged by size:
2024 1606 1274 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 2413 3101 1870
Size: 73 470 688 4545
Fragments arranged by size:
4545 688 470 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2616 2649 2616
Size: 33 5743
BamHI G'GATC_C
Cuts at: 3106 3473 3106
Size: 367 5409
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3097 4552 618
Size: 325 217 627 1310 1455 1842
Fragments arranged by size:
1842 1455 1310 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2841 3095 1391
Size: 1450 254 4072
Fragments arranged by size:
4072 1450 254
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2060 2270 928
Size: 971 161 210 4434
Fragments arranged by size:
4434 971 210 161
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3967 4001 1903
Size: 34 2030 34 3678
Fragments arranged by size:
3678 2030 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3668 4878 3668
Size: 1210 4566
BclI T'GATC_A
Cuts at: 1548 2089 2928 1548
Size: 541 839 4396
Fragments arranged by size:
4396 839 541
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4387 136
Size: 107 122 71 631 605 2715 1525
Fragments arranged by size:
2715 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2319 5771 2319
Size: 3452 2324
BlpI GC'TnA_GC
Cuts at: 3193 3193
Size: 5776
Bme1580I G_kGCm'C
Cuts at: 1790 3837 4993 1790
Size: 2047 1156 2573
Fragments arranged by size:
2573 2047 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5776
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3507 4465 442
Size: 416 53 83 399 24 2090 958 1753
Fragments arranged by size:
2090 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2258 2507 1056
Size: 1202 249 4325
Fragments arranged by size:
4325 1202 249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4436 1239
Size: 3197 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3901 4766 5064 3901
Size: 865 298 4613
Fragments arranged by size:
4613 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4439 882
Size: 617 2940 2219
Fragments arranged by size:
2940 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3567 493
Size: 711 2363 2702
Fragments arranged by size:
2702 2363 711
BsaBI GATnn'nnATC
Cuts at: 5770 5770
Size: 5776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3583 3965 4600 275
Size: 53 83 186 936 2050 382 635 1451
Fragments arranged by size:
2050 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4209 4607 4881 5028 5439 4209
Size: 398 274 147 411 4546
Fragments arranged by size:
4546 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2546 2576 1178
Size: 30 165 30 1143 30 4378
Fragments arranged by size:
4378 1143 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2567 1186
Size: 1381 4395
BseYI C'CCAG_C
Cuts at: 4979 5475 4979
Size: 496 5280
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5203
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3226 3987 5015 1540
Size: 275 186 1225 761 1028 2301
Fragments arranged by size:
2301 1225 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2841 3837 3922 4993 2841
Size: 996 85 1071 3624
Fragments arranged by size:
3624 1071 996 85
BsmI GAATG_Cn'
Cuts at: 2793 3287 3380 2793
Size: 494 93 5189
Fragments arranged by size:
5189 494 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2841 3095 3837 3922 4993 1391
Size: 399 1051 254 742 85 1071 2174
Fragments arranged by size:
2174 1071 1051 742 399 254 85
BspEI T'CCGG_A
Cuts at: 5439 5439
Size: 5776
BspHI T'CATG_A
Cuts at: 3048 3665 5395 3048
Size: 617 1730 3429
Fragments arranged by size:
3429 1730 617
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3131 844
Size: 2287 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3663 1394
Size: 429 1840 3507
Fragments arranged by size:
3507 1840 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4271 4445 65
Size: 1183 3023 174 1396
Fragments arranged by size:
3023 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4420 4607 1700
Size: 110 2610 187 2869
Fragments arranged by size:
2869 2610 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5776
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5776
BssSI C'ACGA_G
Cuts at: 3836 4848 3836
Size: 1012 4764
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5776
BstBI TT'CG_AA
Cuts at: 4580 4580
Size: 5776
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5776
BstYI r'GATC_y
Cuts at: 1972 2319 2708 3011 3106 3473 3858 3875 5327
Size: 347 389 303 95 367 385 17 1452
Cuts at: 5327 5413 5425 5771 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 389 385 367 347 346 303
95 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2457 513
Size: 552 939 453 3832
Fragments arranged by size:
3832 939 552 453
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5037
Fragments arranged by size:
5037 683 56
BtsI GCAGTG_nn'
Cuts at: 3292 4086 4106 3292
Size: 794 20 4962
Fragments arranged by size:
4962 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5741
DraI TTT'AAA
Cuts at: 3037 3080 3037
Size: 43 5733
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4783 809
Size: 507 3467 1802
Fragments arranged by size:
3467 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 3223 4112
Size: 54 1425 50 275 18 270 1123 889
Cuts at: 4112 5741 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1123 889 275 270 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3223 1537
Size: 275 1411 4090
Fragments arranged by size:
4090 1411 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2108 3706 1353
Size: 755 1598 3423
Fragments arranged by size:
3423 1598 755
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2160 4363 4737 4883 387
Size: 1064 138 571 2203 374 146 1280
Fragments arranged by size:
2203 1280 1064 571 374 146 138
EcoICRI GAG'CTC
Cuts at: 2839 2839
Size: 5776
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2308 2410 3839 4436 5223 5491 1239
Size: 393 676 102 1429 597 787 268 1524
Fragments arranged by size:
1524 1429 787 676 597 393 268 102
EcoO109I rG'GnC_Cy
Cuts at: 2625 2625
Size: 5776
EcoRI G'AATT_C
Cuts at: 3085 3463 3085
Size: 378 5398
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 5776
FspI TGC'GCA
Cuts at: 4282 4282
Size: 5776
HaeII r_GCGC'y
Cuts at: 1678 4923 1678
Size: 3245 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2837 2869 4417 4449 4491
Size: 32 135 32 1265 32 1548 32 42
Cuts at: 4491 4523 1373
Size: 32 2626
Fragments arranged by size:
2626 1548 1265 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2077 3120 3366 669
Size: 988 281 139 1043 246 3079
Fragments arranged by size:
3079 1043 988 281 246 139
HindIII A'AGCT_T
Cuts at: 748 2422 2873 748
Size: 1674 451 3651
Fragments arranged by size:
3651 1674 451
HpaI GTT'AAC
Cuts at: 3366 3366
Size: 5776
KpnI G_GTAC'C
Cuts at: 3101 3101
Size: 5776
MfeI C'AATT_G
Cuts at: 3375 3375
Size: 5776
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2195 4890 5074 1305
Size: 890 2695 184 2007
Fragments arranged by size:
2695 2007 890 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3954
Fragments arranged by size:
3954 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2310 2703 3734 4093 4252 518
Size: 648 1144 393 1031 359 159 2042
Fragments arranged by size:
2042 1144 1031 648 393 359 159
MspA1I CmG'CkG
Cuts at: 1606 3192 3869 5017 5262 1606
Size: 1586 677 1148 245 2120
Fragments arranged by size:
2120 1586 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4285
Fragments arranged by size:
4285 939 552
NdeI CA'TA_TG
Cuts at: 387 2194 387
Size: 1807 3969
NheI G'CTAG_C
Cuts at: 1052 2254 2503 1052
Size: 1202 249 4325
Fragments arranged by size:
4325 1202 249
NotI GC'GGCC_GC
Cuts at: 3223 3223
Size: 5776
NspI r_CATG'y
Cuts at: 1132 1229 2276 2517 2831 2861 2943 3134 4679
Size: 97 1047 241 314 30 82 191 1545
Cuts at: 4679 1132
Size: 2229
Fragments arranged by size:
2229 1545 1047 314 241 191 97 82
30
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5776
PciI A'CATG_T
Cuts at: 2827 4675 2827
Size: 1848 3928
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5776
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3845 3877 5358 5390 3845
Size: 32 1481 32 4231
Fragments arranged by size:
4231 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2625 2625
Size: 5776
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5776
PsiI TTA'TAA
Cuts at: 3346 3346
Size: 5776
PstI C_TGCA'G
Cuts at: 830 3128 830
Size: 2298 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
SacI G_AGCT'C
Cuts at: 2841 2841
Size: 5776
SalI G'TCGA_C
Cuts at: 1936 3118 1936
Size: 1182 4594
SapI GCTCTTCn'nnn_
Cuts at: 2108 2108
Size: 5776
SbfI CC_TGCA'GG
Cuts at: 3128 3128
Size: 5776
ScaI AGT'ACT
Cuts at: 4024 5736 4024
Size: 1712 4064
SfcI C'TryA_G
Cuts at: 826 1045 2591 3124 4259 4940 5131 826
Size: 219 1546 533 1135 681 191 1471
Fragments arranged by size:
1546 1471 1135 681 533 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5776
SmaI CCC'GGG
Cuts at: 1872 3103 1872
Size: 1231 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3880 4781 5043 820
Size: 197 926 1937 901 262 1553
Fragments arranged by size:
1937 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 3134 1132
Size: 2002 3774
SspI AAT'ATT
Cuts at: 5 52 2202 3700 5
Size: 47 2150 1498 2081
Fragments arranged by size:
2150 2081 1498 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5776
StyI C'CwwG_G
Cuts at: 513 1065 2004 2181 3215 513
Size: 552 939 177 1034 3074
Fragments arranged by size:
3074 1034 939 552 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3803 3820 3973 4158 4603 325
Size: 1255 2223 17 153 185 445 1498
Fragments arranged by size:
2223 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4022 5734 96
Size: 274 80 33 51 1443 2045 1712 138
Fragments arranged by size:
2045 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2546 2578 5736 5768 2546
Size: 32 3158 32 2554
Fragments arranged by size:
3158 2554 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5776
XbaI T'CTAG_A
Cuts at: 2377 3112 2377
Size: 735 5041
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5776
XmaI C'CCGG_G
Cuts at: 1870 3101 1870
Size: 1231 4545
XmnI GAAnn'nnTTC
Cuts at: 2732 3905 2732
Size: 1173 4603
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI