Restriction map for
pFN21AE0790
(Circular) (Six-base) MAPSORT of: pFN21AE0790.seq Check: 1416 from: 1 to: 6091
pFN21AE0790 6091 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3434 1937
Size: 1497 4594
Acc65I G'GTAC_C
Cuts at: 3412 3412
Size: 6091
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2710 2962 4154 5538 5806 1632
Size: 1078 252 1192 1384 268 1917
Fragments arranged by size:
1917 1384 1192 1078 268 252
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 4990 5967 1203
Size: 3787 977 1327
Fragments arranged by size:
3787 1327 977
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 4820 4820
Size: 6091
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6091
AlwNI CAG_nnn'CTG
Cuts at: 3834 5406 3834
Size: 1572 4519
ApaLI G'TGCA_C
Cuts at: 4148 5304 4148
Size: 1156 4935
ApoI r'AATT_y
Cuts at: 1493 2099 2436 2502 2760 2844 2987 3264 3348
Size: 606 337 66 258 84 143 277 84
Cuts at: 3348 3364 3400 3626 3778 5887 1493
Size: 16 36 226 152 2109 1697
Fragments arranged by size:
2109 1697 606 337 277 258 226 152
143 84 84 66 36 16
AseI AT'TA_AT
Cuts at: 160 1032 4645 160
Size: 872 3613 1606
Fragments arranged by size:
3613 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 3416 1870
Size: 73 1473 4545
Fragments arranged by size:
4545 1473 73
BamHI G'GATC_C
Cuts at: 3421 3788 3421
Size: 367 5724
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3412 4867 618
Size: 325 217 627 1625 1455 1842
Fragments arranged by size:
1842 1625 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2250 3410 1391
Size: 859 1160 4072
Fragments arranged by size:
4072 1160 859
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2195 2716 3304 928
Size: 971 296 521 588 3715
Fragments arranged by size:
3715 971 588 521 296
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4282 4316 1903
Size: 34 50 34 2261 34 3678
Fragments arranged by size:
3678 2261 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3983 5193 3983
Size: 1210 4881
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6091
BfrBI ATG'CAT
Cuts at: 3299 3299
Size: 6091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4702 136
Size: 107 122 71 631 605 3030 1525
Fragments arranged by size:
3030 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6086 6086
Size: 6091
BlpI GC'TnA_GC
Cuts at: 3508 3508
Size: 6091
Bme1580I G_kGCm'C
Cuts at: 1790 4152 5308 1790
Size: 2362 1156 2573
Fragments arranged by size:
2573 2362 1156
BmgBI CAC'GTC
Cuts at: 1318 2089 2158 1318
Size: 771 69 5251
Fragments arranged by size:
5251 771 69
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3822 4780 442
Size: 416 53 83 399 24 2405 958 1753
Fragments arranged by size:
2405 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2874 2958 3210 3294 3378 4751 1239
Size: 1635 84 252 84 84 1373 2579
Fragments arranged by size:
2579 1635 1373 252 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2411 4216 5081 5379 2411
Size: 1805 865 298 3123
Fragments arranged by size:
3123 1805 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3274 4754 882
Size: 617 1775 1480 2219
Fragments arranged by size:
2219 1775 1480 617
BsaAI yAC'GTr
Cuts at: 493 1204 3882 493
Size: 711 2678 2702
Fragments arranged by size:
2702 2678 711
BsaBI GATnn'nnATC
Cuts at: 6085 6085
Size: 6091
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3898 4280 4915 275
Size: 53 83 186 936 2365 382 635 1451
Fragments arranged by size:
2365 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4524 4922 5196 5343 5754 4524
Size: 398 274 147 411 4861
Fragments arranged by size:
4861 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2317 2347 1178
Size: 30 165 30 914 30 4922
Fragments arranged by size:
4922 914 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 2341 1186
Size: 940 215 4936
Fragments arranged by size:
4936 940 215
BseYI C'CCAG_C
Cuts at: 5294 5790 5294
Size: 496 5595
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5518
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3541 4302 5330 1540
Size: 275 186 1540 761 1028 2301
Fragments arranged by size:
2301 1540 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4152 4237 5308 4152
Size: 85 1071 4935
Fragments arranged by size:
4935 1071 85
BsmI GAATG_Cn'
Cuts at: 3602 3695 3602
Size: 93 5998
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2250 3410 4152 4237 5308 1391
Size: 399 460 1160 742 85 1071 2174
Fragments arranged by size:
2174 1160 1071 742 460 399 85
BspEI T'CCGG_A
Cuts at: 5754 5754
Size: 6091
BspHI T'CATG_A
Cuts at: 3980 5710 3980
Size: 1730 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3446 844
Size: 2602 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3978 1394
Size: 429 2155 3507
Fragments arranged by size:
3507 2155 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2353 4586 4760 65
Size: 1183 1105 2233 174 1396
Fragments arranged by size:
2233 1396 1183 1105 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4735 4922 1700
Size: 110 2925 187 2869
Fragments arranged by size:
2925 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6091
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6091
BssSI C'ACGA_G
Cuts at: 4151 5163 4151
Size: 1012 5079
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6091
BstBI TT'CG_AA
Cuts at: 4895 4895
Size: 6091
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6091
BstYI r'GATC_y
Cuts at: 1972 3421 3788 4173 4190 5642 5728 5740 6086
Size: 1449 367 385 17 1452 86 12 346
Cuts at: 6086 1972
Size: 1977
Fragments arranged by size:
1977 1452 1449 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2064 2064
Size: 6091
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5539
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5352
Fragments arranged by size:
5352 683 56
BtsI GCAGTG_nn'
Cuts at: 3368 3607 4401 4421 3368
Size: 239 794 20 5038
Fragments arranged by size:
5038 794 239 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 3395 3395
Size: 6091
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5098 809
Size: 507 3782 1802
Fragments arranged by size:
3782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 3538 4427
Size: 54 1425 50 275 18 261 1447 889
Cuts at: 4427 6056 8
Size: 1629 43
Fragments arranged by size:
1629 1447 1425 889 275 261 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3538 1537
Size: 275 1726 4090
Fragments arranged by size:
4090 1726 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2024 2064 2574 3133 4021 1353
Size: 671 40 510 559 888 3423
Fragments arranged by size:
3423 888 671 559 510 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3038 4678 5052 5198 387
Size: 1064 138 1449 1640 374 146 1280
Fragments arranged by size:
1640 1449 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2710 2874 2958 2962 3210 3294 3378
Size: 393 1078 164 84 4 248 84 84
Cuts at: 3378 4154 4751 5538 5806 1239
Size: 776 597 787 268 1524
Fragments arranged by size:
1524 1078 787 776 597 393 268 248
164 84 84 84 4
EcoRI G'AATT_C
Cuts at: 2099 2760 2844 3264 3364 3400 3778 2099
Size: 661 84 420 100 36 378 4412
Fragments arranged by size:
4412 661 420 378 100 84 36
FspI TGC'GCA
Cuts at: 4597 4597
Size: 6091
HaeII r_GCGC'y
Cuts at: 1678 5238 1678
Size: 3560 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2105 2137 4732 4764 4806
Size: 32 135 32 533 32 2595 32 42
Cuts at: 4806 4838 1373
Size: 32 2626
Fragments arranged by size:
2626 2595 533 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3435 3681 669
Size: 988 281 1497 246 3079
Fragments arranged by size:
3079 1497 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2889 748
Size: 2141 3950
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KpnI G_GTAC'C
Cuts at: 3416 3416
Size: 6091
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2145 5205 5389 1305
Size: 840 3060 184 2007
Fragments arranged by size:
3060 2007 840 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2093 10
Size: 54 1425 343 261 4008
Fragments arranged by size:
4008 1425 343 261 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2087 4049 4408 4567 518
Size: 648 921 1962 359 159 2042
Fragments arranged by size:
2042 1962 921 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2416 3507 4184 5332 5577 1606
Size: 810 1091 677 1148 245 2120
Fragments arranged by size:
2120 1148 1091 810 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5539
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NsiI A_TGCA'T
Cuts at: 3301 3301
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 3303 3449 4994 1132
Size: 97 2074 146 1545 2229
Fragments arranged by size:
2229 2074 1545 146 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6091
PciI A'CATG_T
Cuts at: 4990 4990
Size: 6091
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6091
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6091
PsiI TTA'TAA
Cuts at: 3661 3661
Size: 6091
PstI C_TGCA'G
Cuts at: 830 3443 830
Size: 2613 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
PvuII CAG'CTG
Cuts at: 2416 2416
Size: 6091
SalI G'TCGA_C
Cuts at: 1936 3433 1936
Size: 1497 4594
SapI GCTCTTCn'nnn_
Cuts at: 2064 3133 2064
Size: 1069 5022
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 4339 6051 4339
Size: 1712 4379
SexAI A'CCwGG_T
Cuts at: 2181 2181
Size: 6091
SfcI C'TryA_G
Cuts at: 826 1045 2173 3439 4574 5255 5446 826
Size: 219 1128 1266 1135 681 191 1471
Fragments arranged by size:
1471 1266 1135 1128 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 3418 1872
Size: 1546 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2390 4195 5096 5358 820
Size: 197 926 447 1805 901 262 1553
Fragments arranged by size:
1805 1553 926 901 447 262 197
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 3449 1132
Size: 2317 3774
SspI AAT'ATT
Cuts at: 5 52 4015 5
Size: 47 3963 2081
Fragments arranged by size:
3963 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6091
StyI C'CwwG_G
Cuts at: 513 1065 3530 513
Size: 552 2465 3074
Fragments arranged by size:
3074 2465 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4118 4135 4288 4473 4918 325
Size: 1255 2538 17 153 185 445 1498
Fragments arranged by size:
2538 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2705 3104 4337
Size: 274 80 33 51 1443 728 399 1233
Cuts at: 4337 6049 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1233 728 399 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2317 2349 6051 6083 2317
Size: 32 3702 32 2325
Fragments arranged by size:
3702 2325 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3224 1316
Size: 1908 4183
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 3416 1870
Size: 1546 4545
XmnI GAAnn'nnTTC
Cuts at: 4220 4220
Size: 6091
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI
SfoI SrfI SwaI XcmI