Restriction map for
pFN21AE0788
(Circular) (Six-base) MAPSORT of: pFN21AE0788.seq Check: 4245 from: 1 to: 5962
pFN21AE0788 5962 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2402 2615 3255 2402
Size: 213 640 5109
Fragments arranged by size:
5109 640 213
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2580 2736 3772 278
Size: 53 83 186 936 1044 156 1036 2468
Fragments arranged by size:
2468 1044 1036 936 186 156 83 53
AccI GT'mk_AC
Cuts at: 1937 3305 1937
Size: 1368 4594
Acc65I G'GTAC_C
Cuts at: 3283 3283
Size: 5962
AclI AA'CG_TT
Cuts at: 1563 4089 4462 1563
Size: 2526 373 3063
Fragments arranged by size:
3063 2526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2075 2537 2723 3241 4025 5409 5677 1632
Size: 443 462 186 518 784 1384 268 1917
Fragments arranged by size:
1917 1384 784 518 462 443 268 186
AfeI AGC'GCT
Cuts at: 2339 3149 2339
Size: 810 5152
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5765
AflIII A'CryG_T
Cuts at: 1203 4861 5838 1203
Size: 3658 977 1327
Fragments arranged by size:
3658 1327 977
AgeI A'CCGG_T
Cuts at: 4793 4793
Size: 5962
AhdI GACnn_n'nnGTC
Cuts at: 3064 3175 4691 3064
Size: 111 1516 4335
Fragments arranged by size:
4335 1516 111
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5962
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5962
AlwNI CAG_nnn'CTG
Cuts at: 2032 2290 3705 5277 2032
Size: 258 1415 1572 2717
Fragments arranged by size:
2717 1572 1415 258
ApaI G_GGCC'C
Cuts at: 2642 2642
Size: 5962
ApaLI G'TGCA_C
Cuts at: 4019 5175 4019
Size: 1156 4806
ApoI r'AATT_y
Cuts at: 1493 3271 3497 3649 5758 1493
Size: 1778 226 152 2109 1697
Fragments arranged by size:
2109 1778 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4516 160
Size: 872 3484 1606
Fragments arranged by size:
3484 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 2648 2861 3052 3287 1870
Size: 73 705 213 191 235 4545
Fragments arranged by size:
4545 705 235 213 191 73
BamHI G'GATC_C
Cuts at: 3292 3659 3292
Size: 367 5595
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2390 2603 2857 3283 4738
Size: 325 217 627 603 213 254 426 1455
Cuts at: 4738 618
Size: 1842
Fragments arranged by size:
1842 1455 627 603 426 325 254 217
213
BanII G_rGCy'C
Cuts at: 1391 2186 2235 2598 2642 3024 3281 1391
Size: 795 49 363 44 382 257 4072
Fragments arranged by size:
4072 795 382 363 257 49 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2432 2572 3209 928
Size: 971 533 140 637 3681
Fragments arranged by size:
3681 971 637 533 140
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4153 4187 1903
Size: 34 2216 34 3678
Fragments arranged by size:
3678 2216 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3854 5064 3854
Size: 1210 4752
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5962
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2855 4573
Size: 107 122 71 631 605 337 846 1718
Cuts at: 4573 136
Size: 1525
Fragments arranged by size:
1718 1525 846 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 5957 5957
Size: 5962
BlpI GC'TnA_GC
Cuts at: 3379 3379
Size: 5962
Bme1580I G_kGCm'C
Cuts at: 1790 2606 2642 4023 5179 1790
Size: 816 36 1381 1156 2573
Fragments arranged by size:
2573 1381 1156 816 36
BmgBI CAC'GTC
Cuts at: 1318 2437 3172 1318
Size: 1119 735 4108
Fragments arranged by size:
4108 1119 735
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3693 4651 442
Size: 416 53 83 399 24 2276 958 1753
Fragments arranged by size:
2276 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2384 3182 4622 1239
Size: 1145 798 1440 2579
Fragments arranged by size:
2579 1440 1145 798
Bpu10I CC'TnA_GC
Cuts at: 3233 3233
Size: 5962
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2606 4087 4952 5250 2606
Size: 1481 865 298 3318
Fragments arranged by size:
3318 1481 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3100 4625 882
Size: 617 1601 1525 2219
Fragments arranged by size:
2219 1601 1525 617
BsaAI yAC'GTr
Cuts at: 493 1204 3753 493
Size: 711 2549 2702
Fragments arranged by size:
2702 2549 711
BsaBI GATnn'nnATC
Cuts at: 5956 5956
Size: 5962
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2577 2733 3176
Size: 53 83 186 936 479 565 156 443
Cuts at: 3176 3228 3769 4151 4786 275
Size: 52 541 382 635 1451
Fragments arranged by size:
1451 936 635 565 541 479 443 382
186 156 83 53 52
BsaWI w'CCGG_w
Cuts at: 2421 4395 4793 5067 5214 5625 2421
Size: 1974 398 274 147 411 2758
Fragments arranged by size:
2758 1974 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5737
Fragments arranged by size:
5737 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2492 1186
Size: 1306 4656
BseYI C'CCAG_C
Cuts at: 2112 2227 5165 5661 2112
Size: 115 2938 496 2413
Fragments arranged by size:
2938 2413 496 115
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2564 1163
Size: 573 828 4561
Fragments arranged by size:
4561 828 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2803 3412 4173 5201 1540
Size: 275 186 802 609 761 1028 2301
Fragments arranged by size:
2301 1028 802 761 609 275 186
BsiHKAI G_wGCw'C
Cuts at: 2186 2598 4023 4108 5179 2186
Size: 412 1425 85 1071 2969
Fragments arranged by size:
2969 1425 1071 412 85
BsmI GAATG_Cn'
Cuts at: 2655 3473 3566 2655
Size: 818 93 5051
Fragments arranged by size:
5051 818 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3149 3149
Size: 5962
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2186 2235 2598 2606 2642 3024 3281
Size: 399 396 49 363 8 36 382 257
Cuts at: 3281 4023 4108 5179 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 396 382 363 257
85 49 36 8
BspEI T'CCGG_A
Cuts at: 2421 5625 2421
Size: 3204 2758
BspHI T'CATG_A
Cuts at: 2301 3851 5581 2301
Size: 1550 1730 2682
Fragments arranged by size:
2682 1730 1550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2402 2615 3255 3317 844
Size: 1558 213 640 62 3489
Fragments arranged by size:
3489 1558 640 213 62
BsrBI CCG'CTC
Cuts at: 1394 1823 2166 2764 3849 1394
Size: 429 343 598 1085 3507
Fragments arranged by size:
3507 1085 598 429 343
BsrDI GCAATG_nn'
Cuts at: 65 1248 2439 4457 4631 65
Size: 1183 1191 2018 174 1396
Fragments arranged by size:
2018 1396 1191 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2429 2837 4606 4793 1700
Size: 110 619 408 1769 187 2869
Fragments arranged by size:
2869 1769 619 408 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5962
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5962
BssSI C'ACGA_G
Cuts at: 4022 5034 4022
Size: 1012 4950
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5962
BstBI TT'CG_AA
Cuts at: 4766 4766
Size: 5962
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5962
BstYI r'GATC_y
Cuts at: 1972 3292 3659 4044 4061 5513 5599 5611 5957
Size: 1320 367 385 17 1452 86 12 346
Cuts at: 5957 1972
Size: 1977
Fragments arranged by size:
1977 1452 1320 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2589 3215 513
Size: 552 939 585 626 3260
Fragments arranged by size:
3260 939 626 585 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2569 487
Size: 683 56 1343 3880
Fragments arranged by size:
3880 1343 683 56
BtsI GCAGTG_nn'
Cuts at: 2552 3478 4272 4292 2552
Size: 926 794 20 4222
Fragments arranged by size:
4222 926 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5962
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5927
DraI TTT'AAA
Cuts at: 3266 3266
Size: 5962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2993 4969 809
Size: 507 1677 1976 1802
Fragments arranged by size:
1976 1802 1677 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2398 2431
Size: 54 1425 50 275 18 315 253 33
Cuts at: 2431 2800 3409 4298 5927 8
Size: 369 609 889 1629 43
Fragments arranged by size:
1629 1425 889 609 369 315 275 253
54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2800 3409 1537
Size: 275 988 609 4090
Fragments arranged by size:
4090 988 609 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2513 3012 3098 3892 1353
Size: 1160 499 86 794 3423
Fragments arranged by size:
3423 1160 794 499 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2095 4549 4923 5069 387
Size: 1064 138 506 2454 374 146 1280
Fragments arranged by size:
2454 1280 1064 506 374 146 138
EcoICRI GAG'CTC
Cuts at: 2184 2596 2184
Size: 412 5550
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2384 2537 2723 3182 3241 4025
Size: 393 443 309 153 186 459 59 784
Cuts at: 4025 4622 5409 5677 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 784 597 459 443 393 309
268 186 153 59
EcoNI CCTnn'n_nnAGG
Cuts at: 3256 3256
Size: 5962
EcoO109I rG'GnC_Cy
Cuts at: 2638 2639 2995 2638
Size: 1 356 5605
Fragments arranged by size:
5605 356 1
EcoRI G'AATT_C
Cuts at: 3271 3649 3271
Size: 378 5584
EcoRV GAT'ATC
Cuts at: 2104 2104
Size: 5962
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2434 3004 3101 2434
Size: 570 97 5295
Fragments arranged by size:
5295 570 97
FspI TGC'GCA
Cuts at: 4468 4468
Size: 5962
HaeII r_GCGC'y
Cuts at: 1678 2341 2464 2693 2838 3151 5109 1678
Size: 663 123 229 145 313 1958 2531
Fragments arranged by size:
2531 1958 663 313 229 145 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3050 3082 3161 3193 4603
Size: 32 135 32 1478 32 79 32 1410
Cuts at: 4603 4635 4677 4709 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1478 1410 135 79 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2241 3306 3552 669
Size: 988 281 303 1065 246 3079
Fragments arranged by size:
3079 1065 988 303 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5962
HpaI GTT'AAC
Cuts at: 3552 3552
Size: 5962
KpnI G_GTAC'C
Cuts at: 3287 3287
Size: 5962
MfeI C'AATT_G
Cuts at: 3561 3561
Size: 5962
MluI A'CGCG_T
Cuts at: 5838 5838
Size: 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2769 5076 5260 1305
Size: 1464 2307 184 2007
Fragments arranged by size:
2307 2007 1464 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2147 10
Size: 54 1425 343 315 3825
Fragments arranged by size:
3825 1425 343 315 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2495 2588 3920 4279 4438 518
Size: 648 1329 93 1332 359 159 2042
Fragments arranged by size:
2042 1332 1329 648 359 159 93
MspA1I CmG'CkG
Cuts at: 1606 2116 2143 2388 2799 3217 3378 4055 5203
Size: 510 27 245 411 418 161 677 1148
Cuts at: 5203 5448 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 510 418 411 245 245
161 27
NaeI GCC'GGC
Cuts at: 2839 2839
Size: 5962
NcoI C'CATG_G
Cuts at: 513 1065 2004 2589 513
Size: 552 939 585 3886
Fragments arranged by size:
3886 939 585 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5962
NgoMIV G'CCGG_C
Cuts at: 2837 2837
Size: 5962
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5962
NotI GC'GGCC_GC
Cuts at: 2800 3409 2800
Size: 609 5353
NspI r_CATG'y
Cuts at: 1132 1229 2227 3320 4865 1132
Size: 97 998 1093 1545 2229
Fragments arranged by size:
2229 1545 1093 998 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5962
PciI A'CATG_T
Cuts at: 4861 4861
Size: 5962
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2227 1108
Size: 1119 4843
PfoI T'CCnGG_A
Cuts at: 2465 2465
Size: 5962
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 5544 5576 4031
Size: 32 1481 32 4417
Fragments arranged by size:
4417 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2995 2995
Size: 5962
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5962
PsiI TTA'TAA
Cuts at: 3532 3532
Size: 5962
PspOMI G'GGCC_C
Cuts at: 2638 2638
Size: 5962
PstI C_TGCA'G
Cuts at: 830 2136 2295 3314 830
Size: 1306 159 1019 3478
Fragments arranged by size:
3478 1306 1019 159
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
SacI G_AGCT'C
Cuts at: 2186 2598 2186
Size: 412 5550
SacII CC_GC'GG
Cuts at: 3218 3218
Size: 5962
SalI G'TCGA_C
Cuts at: 1936 3304 1936
Size: 1368 4594
SapI GCTCTTCn'nnn_
Cuts at: 2513 3012 2513
Size: 499 5463
SbfI CC_TGCA'GG
Cuts at: 3314 3314
Size: 5962
ScaI AGT'ACT
Cuts at: 4210 5922 4210
Size: 1712 4250
SfcI C'TryA_G
Cuts at: 826 1045 2132 2291 3011 3310 4445 5126 5317
Size: 219 1087 159 720 299 1135 681 191
Cuts at: 5317 826
Size: 1471
Fragments arranged by size:
1471 1135 1087 720 681 299 219 191
159
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5962
SmaI CCC'GGG
Cuts at: 1872 3289 1872
Size: 1417 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2621 4066 4967 5229 820
Size: 197 926 678 1445 901 262 1553
Fragments arranged by size:
1553 1445 926 901 678 262 197
SnaBI TAC'GTA
Cuts at: 493 3753 493
Size: 3260 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5962
SphI G_CATG'C
Cuts at: 1132 3320 1132
Size: 2188 3774
SspI AAT'ATT
Cuts at: 5 52 3886 5
Size: 47 3834 2081
Fragments arranged by size:
3834 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5962
StyI C'CwwG_G
Cuts at: 513 1065 2004 2589 3401 513
Size: 552 939 585 812 3074
Fragments arranged by size:
3074 939 812 585 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3989 4006 4159 4344 4789 325
Size: 1255 2409 17 153 185 445 1498
Fragments arranged by size:
2409 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2694 4208 5920
Size: 274 80 33 51 1443 717 1514 1712
Cuts at: 5920 96
Size: 138
Fragments arranged by size:
1712 1514 1443 717 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5922 5954 5922
Size: 32 5930
Tth111I GACn'n_nGTC
Cuts at: 1316 3208 1316
Size: 1892 4070
XbaI T'CTAG_A
Cuts at: 3298 3298
Size: 5962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2596 2752 2596
Size: 156 5806
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5962
XmaI C'CCGG_G
Cuts at: 1870 3287 1870
Size: 1417 4545
XmnI GAAnn'nnTTC
Cuts at: 4091 4091
Size: 5962
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2578 2734 3770 276
Size: 53 83 186 936 1044 156 1036 2468
Fragments arranged by size:
2468 1044 1036 936 186 156 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SacII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI PvuII
RsrII SanDI SexAI SfiI SfoI SrfI SwaI