Restriction map for
pFN21AE0783
(Circular) (Six-base) MAPSORT of: pFN21AE0783.seq Check: 2126 from: 1 to: 5761
pFN21AE0783 5761 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3571 278
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3104 1937
Size: 1167 4594
Acc65I G'GTAC_C
Cuts at: 3082 3082
Size: 5761
AclI AA'CG_TT
Cuts at: 1563 3888 4261 1563
Size: 2325 373 3063
Fragments arranged by size:
3063 2325 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3824 5208 5476 1632
Size: 2192 1384 268 1917
Fragments arranged by size:
2192 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5564
AflIII A'CryG_T
Cuts at: 1203 4660 5637 1203
Size: 3457 977 1327
Fragments arranged by size:
3457 1327 977
AgeI A'CCGG_T
Cuts at: 4592 4592
Size: 5761
AhdI GACnn_n'nnGTC
Cuts at: 4490 4490
Size: 5761
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5761
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2378 1184
Size: 1194 4567
AlwNI CAG_nnn'CTG
Cuts at: 2161 2235 2738 2999 3504 5076 2161
Size: 74 503 261 505 1572 2846
Fragments arranged by size:
2846 1572 505 503 261 74
ApaLI G'TGCA_C
Cuts at: 2060 3818 4974 2060
Size: 1758 1156 2847
Fragments arranged by size:
2847 1758 1156
ApoI r'AATT_y
Cuts at: 1493 3070 3296 3448 5557 1493
Size: 1577 226 152 2109 1697
Fragments arranged by size:
2109 1697 1577 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4315 160
Size: 872 3283 1606
Fragments arranged by size:
3283 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
AvaI C'yCGr_G
Cuts at: 1870 1943 2099 2282 2636 2782 3086 1870
Size: 73 156 183 354 146 304 4545
Fragments arranged by size:
4545 354 304 183 156 146 73
BamHI G'GATC_C
Cuts at: 2165 2389 2773 2803 3091 3458 2165
Size: 224 384 30 288 367 4468
Fragments arranged by size:
4468 384 367 288 224 30
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2848 2931 3082 4537 618
Size: 325 217 627 1061 83 151 1455 1842
Fragments arranged by size:
1842 1455 1061 627 325 217 151 83
BanII G_rGCy'C
Cuts at: 1391 2953 3080 1391
Size: 1562 127 4072
Fragments arranged by size:
4072 1562 127
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4790
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2432 2466 3952 3986 1903
Size: 34 495 34 1486 34 3678
Fragments arranged by size:
3678 1486 495 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3653 4863 3653
Size: 1210 4551
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5761
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2199 2380 4372
Size: 107 122 71 631 605 527 181 1992
Cuts at: 4372 136
Size: 1525
Fragments arranged by size:
1992 1525 631 605 527 181 122 107
71
BglII A'GATC_T
Cuts at: 5756 5756
Size: 5761
BlpI GC'TnA_GC
Cuts at: 3178 3178
Size: 5761
Bme1580I G_kGCm'C
Cuts at: 1790 2064 3822 4978 1790
Size: 274 1758 1156 2573
Fragments arranged by size:
2573 1758 1156 274
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5761
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2171 3492 4450
Size: 416 53 83 399 24 754 1321 958
Cuts at: 4450 442
Size: 1753
Fragments arranged by size:
1753 1321 958 754 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5761
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2497 2497
Size: 5761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2168 2635 4421 1239
Size: 929 467 1786 2579
Fragments arranged by size:
2579 1786 929 467
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2107 3886 4751 5049 2107
Size: 1779 865 298 2819
Fragments arranged by size:
2819 1779 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2591 4424 882
Size: 617 1092 1833 2219
Fragments arranged by size:
2219 1833 1092 617
BsaAI yAC'GTr
Cuts at: 493 1204 3552 493
Size: 711 2348 2702
Fragments arranged by size:
2702 2348 711
BsaBI GATnn'nnATC
Cuts at: 5755 5755
Size: 5761
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2313 3568 3950 4585
Size: 53 83 186 936 780 1255 382 635
Cuts at: 4585 275
Size: 1451
Fragments arranged by size:
1451 1255 936 780 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2712 2744 4194 4592 4866 5013 5424 2712
Size: 32 1450 398 274 147 411 3049
Fragments arranged by size:
3049 1450 411 398 274 147 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2195 2225 1178
Size: 30 165 30 792 30 4714
Fragments arranged by size:
4714 792 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2489 2632 3035 1186
Size: 1303 143 403 3912
Fragments arranged by size:
3912 1303 403 143
BseYI C'CCAG_C
Cuts at: 4964 5460 4964
Size: 496 5265
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5188
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3211 3972 5000 1540
Size: 275 186 1210 761 1028 2301
Fragments arranged by size:
2301 1210 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2064 2982 3822 3907 4978 2064
Size: 918 840 85 1071 2847
Fragments arranged by size:
2847 1071 918 840 85
BsmI GAATG_Cn'
Cuts at: 3272 3365 3272
Size: 93 5668
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2953 2982 3080 3822 3907 4978
Size: 399 274 889 29 98 742 85 1071
Cuts at: 4978 1391
Size: 2174
Fragments arranged by size:
2174 1071 889 742 399 274 98 85
29
BspEI T'CCGG_A
Cuts at: 2712 5424 2712
Size: 2712 3049
BspHI T'CATG_A
Cuts at: 2274 3650 5380 2274
Size: 1376 1730 2655
Fragments arranged by size:
2655 1730 1376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3116 844
Size: 2272 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3648 1394
Size: 429 1825 3507
Fragments arranged by size:
3507 1825 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4256 4430 65
Size: 1183 3008 174 1396
Fragments arranged by size:
3008 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4405 4592 1700
Size: 110 2595 187 2869
Fragments arranged by size:
2869 2595 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5761
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5761
BssSI C'ACGA_G
Cuts at: 3821 4833 3821
Size: 1012 4749
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2235 794
Size: 1441 4320
BstBI TT'CG_AA
Cuts at: 4565 4565
Size: 5761
BstEII G'GTnAC_C
Cuts at: 1184 2320 2908 1184
Size: 1136 588 4037
Fragments arranged by size:
4037 1136 588
BstXI CCAn_nnnn'nTGG
Cuts at: 2437 2437
Size: 5761
BstYI r'GATC_y
Cuts at: 1972 2165 2389 2413 2717 2773 2803 3091 3458
Size: 193 224 24 304 56 30 288 367
Cuts at: 3458 3843 3860 5312 5398 5410 5756 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 304 288 224
193 86 56 30 24 17 12
Bsu36I CC'TnA_GG
Cuts at: 2472 2472
Size: 5761
BtgI C'CryG_G
Cuts at: 513 1065 2042 2110 2721 513
Size: 552 977 68 611 3553
Fragments arranged by size:
3553 977 611 552 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5022
Fragments arranged by size:
5022 683 56
BtsI GCAGTG_nn'
Cuts at: 2442 3277 4071 4091 2442
Size: 835 794 20 4112
Fragments arranged by size:
4112 835 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5726
DraI TTT'AAA
Cuts at: 3065 3065
Size: 5761
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2318 2864 4768 809
Size: 507 1002 546 1904 1802
Fragments arranged by size:
1904 1802 1002 546 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2439 2814 3208
Size: 54 1425 50 275 18 609 375 394
Cuts at: 3208 4097 5726 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 609 394 375 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3208 1537
Size: 275 1396 4090
Fragments arranged by size:
4090 1396 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3691 1353
Size: 2338 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4348 4722 4868 387
Size: 1064 138 2759 374 146 1280
Fragments arranged by size:
2759 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 2635 3824 4421 5208 5476 1239
Size: 393 536 467 1189 597 787 268 1524
Fragments arranged by size:
1524 1189 787 597 536 467 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2095 2735 2866 2095
Size: 640 131 4990
Fragments arranged by size:
4990 640 131
EcoRI G'AATT_C
Cuts at: 3070 3448 3070
Size: 378 5383
FspI TGC'GCA
Cuts at: 4267 4267
Size: 5761
HaeII r_GCGC'y
Cuts at: 1678 4908 1678
Size: 3230 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2157 2189 2765 2797 4402
Size: 32 135 32 585 32 576 32 1605
Cuts at: 4402 4434 4476 4508 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1605 585 576 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3105 3351 669
Size: 988 281 1167 246 3079
Fragments arranged by size:
3079 1167 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5761
HpaI GTT'AAC
Cuts at: 3351 3351
Size: 5761
KpnI G_GTAC'C
Cuts at: 3086 3086
Size: 5761
MfeI C'AATT_G
Cuts at: 2343 3360 2343
Size: 1017 4744
MluI A'CGCG_T
Cuts at: 5637 5637
Size: 5761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2454 4875 5059 1305
Size: 1149 2421 184 2007
Fragments arranged by size:
2421 2007 1149 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2816 10
Size: 54 1425 343 984 2955
Fragments arranged by size:
2955 1425 984 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2069 2193 2906 3719 4078 4237 518
Size: 648 903 124 713 813 359 159 2042
Fragments arranged by size:
2042 903 813 713 648 359 159 124
MspA1I CmG'CkG
Cuts at: 1606 2080 2232 2898 3177 3854 5002 5247 1606
Size: 474 152 666 279 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 666 474 279 245 152
NcoI C'CATG_G
Cuts at: 513 1065 2110 513
Size: 552 1045 4164
Fragments arranged by size:
4164 1045 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5761
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5761
NotI GC'GGCC_GC
Cuts at: 3208 3208
Size: 5761
NspI r_CATG'y
Cuts at: 1132 1229 3119 4664 1132
Size: 97 1890 1545 2229
Fragments arranged by size:
2229 1890 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2738 2915 3050 1348
Size: 1390 177 135 4059
Fragments arranged by size:
4059 1390 177 135
PciI A'CATG_T
Cuts at: 4660 4660
Size: 5761
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2245 2989 1108
Size: 1137 744 3880
Fragments arranged by size:
3880 1137 744
PfoI T'CCnGG_A
Cuts at: 3015 3015
Size: 5761
PmeI GTTT'AAAC
Cuts at: 3065 3065
Size: 5761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3862 5343 5375 3830
Size: 32 1481 32 4216
Fragments arranged by size:
4216 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2095 2866 2095
Size: 771 4990
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5761
PsiI TTA'TAA
Cuts at: 3331 3331
Size: 5761
PstI C_TGCA'G
Cuts at: 830 2381 3113 830
Size: 1551 732 3478
Fragments arranged by size:
3478 1551 732
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5761
PvuII CAG'CTG
Cuts at: 2080 2232 2898 2080
Size: 152 666 4943
Fragments arranged by size:
4943 666 152
SalI G'TCGA_C
Cuts at: 1936 3103 1936
Size: 1167 4594
SanDI GG'GwC_CC
Cuts at: 2095 2095
Size: 5761
SbfI CC_TGCA'GG
Cuts at: 2381 3113 2381
Size: 732 5029
ScaI AGT'ACT
Cuts at: 4009 5721 4009
Size: 1712 4049
SfcI C'TryA_G
Cuts at: 826 1045 2377 3109 4244 4925 5116 826
Size: 219 1332 732 1135 681 191 1471
Fragments arranged by size:
1471 1332 1135 732 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5761
SmaI CCC'GGG
Cuts at: 1872 2284 3088 1872
Size: 412 804 4545
Fragments arranged by size:
4545 804 412
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2122 3865 4766 5028 820
Size: 197 926 179 1743 901 262 1553
Fragments arranged by size:
1743 1553 926 901 262 197 179
SnaBI TAC'GTA
Cuts at: 493 3552 493
Size: 3059 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5761
SphI G_CATG'C
Cuts at: 1132 3119 1132
Size: 1987 3774
SspI AAT'ATT
Cuts at: 5 52 3685 5
Size: 47 3633 2081
Fragments arranged by size:
3633 2081 47
StuI AGG'CCT
Cuts at: 1517 2480 1517
Size: 963 4798
StyI C'CwwG_G
Cuts at: 513 1065 2110 2553 3200 513
Size: 552 1045 443 647 3074
Fragments arranged by size:
3074 1045 647 552 443
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2881 3788 3805 3958 4143 4588 325
Size: 1255 1301 907 17 153 185 445 1498
Fragments arranged by size:
1498 1301 1255 907 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4007 5719 96
Size: 274 80 33 51 1443 2030 1712 138
Fragments arranged by size:
2030 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5721 5753 5721
Size: 32 5729
Tth111I GACn'n_nGTC
Cuts at: 1316 2093 1316
Size: 777 4984
XbaI T'CTAG_A
Cuts at: 3097 3097
Size: 5761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2049 2049
Size: 5761
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5761
XmaI C'CCGG_G
Cuts at: 1870 2282 3086 1870
Size: 412 804 4545
Fragments arranged by size:
4545 804 412
XmnI GAAnn'nnTTC
Cuts at: 3890 3890
Size: 5761
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3569 276
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI