Restriction map for
pFN21AE0781
(Circular) (Six-base) MAPSORT of: pFN21AE0781.seq Check: 4772 from: 1 to: 5755
pFN21AE0781 5755 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3565 278
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3098 1937
Size: 1161 4594
Acc65I G'GTAC_C
Cuts at: 3076 3076
Size: 5755
AclI AA'CG_TT
Cuts at: 1563 3882 4255 1563
Size: 2319 373 3063
Fragments arranged by size:
3063 2319 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2780 2883 3818 5202 5470 1632
Size: 1148 103 935 1384 268 1917
Fragments arranged by size:
1917 1384 1148 935 268 103
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 2589 4654 5631 1203
Size: 1386 2065 977 1327
Fragments arranged by size:
2065 1386 1327 977
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 4484 4484
Size: 5755
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5755
AlwNI CAG_nnn'CTG
Cuts at: 3498 5070 3498
Size: 1572 4183
ApaLI G'TGCA_C
Cuts at: 2972 3812 4968 2972
Size: 840 1156 3759
Fragments arranged by size:
3759 1156 840
ApoI r'AATT_y
Cuts at: 1493 2105 2327 2689 2723 3064 3290 3442 5551
Size: 612 222 362 34 341 226 152 2109
Cuts at: 5551 1493
Size: 1697
Fragments arranged by size:
2109 1697 612 362 341 226 222 152
34
AseI AT'TA_AT
Cuts at: 160 1032 4309 160
Size: 872 3277 1606
Fragments arranged by size:
3277 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 3080 1870
Size: 73 1137 4545
Fragments arranged by size:
4545 1137 73
BamHI G'GATC_C
Cuts at: 3085 3452 3085
Size: 367 5388
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2384 3076 4531 618
Size: 325 217 627 597 692 1455 1842
Fragments arranged by size:
1842 1455 692 627 597 325 217
BanII G_rGCy'C
Cuts at: 1391 3074 1391
Size: 1683 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2820 928
Size: 971 921 3863
Fragments arranged by size:
3863 971 921
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3946 3980 1903
Size: 34 2009 34 3678
Fragments arranged by size:
3678 2009 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2512 3647 4857 2512
Size: 1135 1210 3410
Fragments arranged by size:
3410 1210 1135
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5755
BfrBI ATG'CAT
Cuts at: 2310 2310
Size: 5755
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2532 4366 136
Size: 107 122 71 631 605 860 1834 1525
Fragments arranged by size:
1834 1525 860 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5750 5750
Size: 5755
BlpI GC'TnA_GC
Cuts at: 3172 3172
Size: 5755
Bme1580I G_kGCm'C
Cuts at: 1790 2976 3816 4972 1790
Size: 1186 840 1156 2573
Fragments arranged by size:
2573 1186 1156 840
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5755
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3057 3486 4444
Size: 416 53 83 399 24 1640 429 958
Cuts at: 4444 442
Size: 1753
Fragments arranged by size:
1753 1640 958 429 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2929 2929
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2282 2604 4415 1239
Size: 1043 322 1811 2579
Fragments arranged by size:
2579 1811 1043 322
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2827 2899 3880 4745 5043 2827
Size: 72 981 865 298 3539
Fragments arranged by size:
3539 981 865 298 72
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4418 882
Size: 617 2919 2219
Fragments arranged by size:
2919 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2064 3546 493
Size: 711 860 1482 2702
Fragments arranged by size:
2702 1482 860 711
BsaBI GATnn'nnATC
Cuts at: 5749 5749
Size: 5755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3562 3944 4579 275
Size: 53 83 186 936 2029 382 635 1451
Fragments arranged by size:
2029 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4188 4586 4860 5007 5418 4188
Size: 398 274 147 411 4525
Fragments arranged by size:
4525 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5530
Fragments arranged by size:
5530 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2279 1186
Size: 1093 4662
BseYI C'CCAG_C
Cuts at: 2846 4958 5454 2846
Size: 2112 496 3147
Fragments arranged by size:
3147 2112 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2622 2813 1163
Size: 573 886 191 4105
Fragments arranged by size:
4105 886 573 191
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3205 3966 4994 1540
Size: 275 186 1204 761 1028 2301
Fragments arranged by size:
2301 1204 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2165 2976 3816 3901 4972 2165
Size: 811 840 85 1071 2948
Fragments arranged by size:
2948 1071 840 811 85
BsmI GAATG_Cn'
Cuts at: 3266 3359 3266
Size: 93 5662
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2165 2976 3074 3816 3901 4972 1391
Size: 399 375 811 98 742 85 1071 2174
Fragments arranged by size:
2174 1071 811 742 399 375 98 85
BspEI T'CCGG_A
Cuts at: 5418 5418
Size: 5755
BspHI T'CATG_A
Cuts at: 2538 3644 5374 2538
Size: 1106 1730 2919
Fragments arranged by size:
2919 1730 1106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2835 3110 844
Size: 1991 275 3489
Fragments arranged by size:
3489 1991 275
BsrBI CCG'CTC
Cuts at: 1394 1823 3642 1394
Size: 429 1819 3507
Fragments arranged by size:
3507 1819 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4250 4424 65
Size: 1183 3002 174 1396
Fragments arranged by size:
3002 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4399 4586 1700
Size: 110 2589 187 2869
Fragments arranged by size:
2869 2589 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5755
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5755
BssSI C'ACGA_G
Cuts at: 3815 4827 3815
Size: 1012 4743
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5755
BstBI TT'CG_AA
Cuts at: 4559 4559
Size: 5755
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5755
BstXI CCAn_nnnn'nTGG
Cuts at: 3034 3034
Size: 5755
BstYI r'GATC_y
Cuts at: 1972 2857 3085 3452 3837 3854 5306 5392 5404
Size: 885 228 367 385 17 1452 86 12
Cuts at: 5404 5750 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 885 385 367 346 228 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4264
Fragments arranged by size:
4264 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2535 487
Size: 683 56 1309 3707
Fragments arranged by size:
3707 1309 683 56
BtsI GCAGTG_nn'
Cuts at: 2464 2610 3271 4065 4085 2464
Size: 146 661 794 20 4134
Fragments arranged by size:
4134 794 661 146 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DraIII CAC_nnn'GTG
Cuts at: 3015 3015
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4762 809
Size: 507 3446 1802
Fragments arranged by size:
3446 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3202 4091 5720
Size: 54 1425 50 275 18 1372 889 1629
Cuts at: 5720 8
Size: 43
Fragments arranged by size:
1629 1425 1372 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3202 1537
Size: 275 1390 4090
Fragments arranged by size:
4090 1390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2679 2921 2931 3685 1353
Size: 1326 242 10 754 3423
Fragments arranged by size:
3423 1326 754 242 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4342 4716 4862 387
Size: 1064 138 2753 374 146 1280
Fragments arranged by size:
2753 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2282 2604 2780 2883 3818 4415 5202
Size: 393 650 322 176 103 935 597 787
Cuts at: 5202 5470 1239
Size: 268 1524
Fragments arranged by size:
1524 935 787 650 597 393 322 268
176 103
EcoRI G'AATT_C
Cuts at: 2105 2689 2723 3064 3442 2105
Size: 584 34 341 378 4418
Fragments arranged by size:
4418 584 378 341 34
EcoRV GAT'ATC
Cuts at: 2290 2290
Size: 5755
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2821 2960 2821
Size: 139 5616
FspI TGC'GCA
Cuts at: 4261 4261
Size: 5755
HaeII r_GCGC'y
Cuts at: 1678 4902 1678
Size: 3224 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2446 2478 4396 4428 4470
Size: 32 135 32 874 32 1918 32 42
Cuts at: 4470 4502 1373
Size: 32 2626
Fragments arranged by size:
2626 1918 874 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3099 3345 669
Size: 988 281 1161 246 3079
Fragments arranged by size:
3079 1161 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KpnI G_GTAC'C
Cuts at: 3080 3080
Size: 5755
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2529 4869 5053 1305
Size: 1224 2340 184 2007
Fragments arranged by size:
2340 2007 1224 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3933
Fragments arranged by size:
3933 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2835 3032 3713 4072 4231 518
Size: 648 1669 197 681 359 159 2042
Fragments arranged by size:
2042 1669 681 648 359 197 159
MspA1I CmG'CkG
Cuts at: 1606 2152 3171 3848 4996 5241 1606
Size: 546 1019 677 1148 245 2120
Fragments arranged by size:
2120 1148 1019 677 546 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4264
Fragments arranged by size:
4264 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NsiI A_TGCA'T
Cuts at: 2312 2312
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 2593 3113 4658 1132
Size: 97 1364 520 1545 2229
Fragments arranged by size:
2229 1545 1364 520 97
PasI CC'CwG_GG
Cuts at: 1348 2863 1348
Size: 1515 4240
PciI A'CATG_T
Cuts at: 2589 4654 2589
Size: 2065 3690
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2669 1108
Size: 1561 4194
PfoI T'CCnGG_A
Cuts at: 2259 2631 2259
Size: 372 5383
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 5337 5369 3824
Size: 32 1481 32 4210
Fragments arranged by size:
4210 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5755
PsiI TTA'TAA
Cuts at: 3325 3325
Size: 5755
PstI C_TGCA'G
Cuts at: 830 2577 3107 830
Size: 1747 530 3478
Fragments arranged by size:
3478 1747 530
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SfcI C'TryA_G
Cuts at: 826 1045 2573 3103 4238 4919 5110 826
Size: 219 1528 530 1135 681 191 1471
Fragments arranged by size:
1528 1471 1135 681 530 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5755
SmaI CCC'GGG
Cuts at: 1872 3082 1872
Size: 1210 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2806 2878 3859 4760 5022 820
Size: 197 926 863 72 981 901 262 1553
Fragments arranged by size:
1553 981 926 901 863 262 197 72
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 3113 1132
Size: 1981 3774
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 2535 1517
Size: 1018 4737
StyI C'CwwG_G
Cuts at: 513 1065 2004 3000 3194 513
Size: 552 939 996 194 3074
Fragments arranged by size:
3074 996 939 552 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3782 3799 3952 4137 4582 325
Size: 1255 2202 17 153 185 445 1498
Fragments arranged by size:
2202 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4001 5713 96
Size: 274 80 33 51 1443 2024 1712 138
Fragments arranged by size:
2024 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5715 5747 5715
Size: 32 5723
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5755
XbaI T'CTAG_A
Cuts at: 2437 3091 2437
Size: 654 5101
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2602 2602
Size: 5755
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 3080 1870
Size: 1210 4545
XmnI GAAnn'nnTTC
Cuts at: 3884 3884
Size: 5755
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3563 276
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI