Restriction map for
pFN21AE0778
(Circular) (Six-base) MAPSORT of: pFN21AE0778.seq Check: 488 from: 1 to: 6247
pFN21AE0778 6247 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4057 278
Size: 53 83 186 936 2521 2468
Fragments arranged by size:
2521 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3350 3590 1937
Size: 1413 240 4594
Fragments arranged by size:
4594 1413 240
Acc65I G'GTAC_C
Cuts at: 3568 3568
Size: 6247
AclI AA'CG_TT
Cuts at: 1563 4374 4747 1563
Size: 2811 373 3063
Fragments arranged by size:
3063 2811 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2901 3288 3309 4310 5694 5962 1632
Size: 1269 387 21 1001 1384 268 1917
Fragments arranged by size:
1917 1384 1269 1001 387 268 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6050
AflIII A'CryG_T
Cuts at: 1203 2852 2946 5146 6123 1203
Size: 1649 94 2200 977 1327
Fragments arranged by size:
2200 1649 1327 977 94
AgeI A'CCGG_T
Cuts at: 5078 5078
Size: 6247
AhdI GACnn_n'nnGTC
Cuts at: 4976 4976
Size: 6247
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6247
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3390 1184
Size: 2206 4041
AlwNI CAG_nnn'CTG
Cuts at: 3990 5562 3990
Size: 1572 4675
ApaLI G'TGCA_C
Cuts at: 4304 5460 4304
Size: 1156 5091
ApoI r'AATT_y
Cuts at: 1493 2610 3046 3556 3782 3934 6043 1493
Size: 1117 436 510 226 152 2109 1697
Fragments arranged by size:
2109 1697 1117 510 436 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4801 160
Size: 872 3769 1606
Fragments arranged by size:
3769 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
AvaI C'yCGr_G
Cuts at: 1870 1943 3572 1870
Size: 73 1629 4545
Fragments arranged by size:
4545 1629 73
BamHI G'GATC_C
Cuts at: 3577 3944 3577
Size: 367 5880
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3518 3568 5023 618
Size: 325 217 627 1731 50 1455 1842
Fragments arranged by size:
1842 1731 1455 627 325 217 50
BanII G_rGCy'C
Cuts at: 1391 3566 1391
Size: 2175 4072
BbeI G_GCGC'C
Cuts at: 3522 3522
Size: 6247
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5276
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2917 2951 4438 4472 1903
Size: 34 980 34 1487 34 3678
Fragments arranged by size:
3678 1487 980 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4139 5349 4139
Size: 1210 5037
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6247
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4858 136
Size: 107 122 71 631 605 3186 1525
Fragments arranged by size:
3186 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2716 6242 2716
Size: 3526 2721
BlpI GC'TnA_GC
Cuts at: 2806 3664 2806
Size: 858 5389
Bme1580I G_kGCm'C
Cuts at: 1790 2896 4308 5464 1790
Size: 1106 1412 1156 2573
Fragments arranged by size:
2573 1412 1156 1106
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6247
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3978 4936 442
Size: 416 53 83 399 24 2561 958 1753
Fragments arranged by size:
2561 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2094 2195 2200 4907 1239
Size: 855 101 5 2707 2579
Fragments arranged by size:
2707 2579 855 101 5
Bpu10I CC'TnA_GC
Cuts at: 3120 3120
Size: 6247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2218 4372 5237 5535 2218
Size: 2154 865 298 2930
Fragments arranged by size:
2930 2154 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3166 4910 882
Size: 617 1667 1744 2219
Fragments arranged by size:
2219 1744 1667 617
BsaAI yAC'GTr
Cuts at: 493 1204 2957 4038 493
Size: 711 1753 1081 2702
Fragments arranged by size:
2702 1753 1081 711
BsaBI GATnn'nnATC
Cuts at: 6241 6241
Size: 6247
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2934 3519 4054 4436
Size: 53 83 186 936 1401 585 535 382
Cuts at: 4436 5071 275
Size: 635 1451
Fragments arranged by size:
1451 1401 936 635 585 535 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4680 5078 5352 5499 5910 4680
Size: 398 274 147 411 5017
Fragments arranged by size:
5017 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6022
Fragments arranged by size:
6022 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3350 1186
Size: 2164 4083
BseYI C'CCAG_C
Cuts at: 5450 5946 5450
Size: 496 5751
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2175 3424 1163
Size: 573 439 1249 3986
Fragments arranged by size:
3986 1249 573 439
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3697 4458 5486 1540
Size: 275 186 1696 761 1028 2301
Fragments arranged by size:
2301 1696 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4308 4393 5464 4308
Size: 85 1071 5091
Fragments arranged by size:
5091 1071 85
BsmI GAATG_Cn'
Cuts at: 3758 3851 3758
Size: 93 6154
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2896 3566 4308 4393 5464 1391
Size: 399 1106 670 742 85 1071 2174
Fragments arranged by size:
2174 1106 1071 742 670 399 85
BspEI T'CCGG_A
Cuts at: 5910 5910
Size: 6247
BspHI T'CATG_A
Cuts at: 4136 5866 4136
Size: 1730 4517
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3602 844
Size: 2758 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4134 1394
Size: 429 2311 3507
Fragments arranged by size:
3507 2311 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4742 4916 65
Size: 1183 3494 174 1396
Fragments arranged by size:
3494 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4891 5078 1700
Size: 110 3081 187 2869
Fragments arranged by size:
3081 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2707 2943 96
Size: 2611 236 3400
Fragments arranged by size:
3400 2611 236
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6247
BssSI C'ACGA_G
Cuts at: 4307 5319 4307
Size: 1012 5235
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6247
BstBI TT'CG_AA
Cuts at: 5051 5051
Size: 6247
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6247
BstYI r'GATC_y
Cuts at: 1972 2716 3097 3577 3944 4329 4346 5798 5884
Size: 744 381 480 367 385 17 1452 86
Cuts at: 5884 5896 6242 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 744 480 385 381 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4756
Fragments arranged by size:
4756 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5508
Fragments arranged by size:
5508 683 56
BtsI GCAGTG_nn'
Cuts at: 2918 3763 4557 4577 2918
Size: 845 794 20 4588
Fragments arranged by size:
4588 845 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6247
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2955 2990 529
Size: 35 2391 35 3786
Fragments arranged by size:
3786 2391 35 35
DraI TTT'AAA
Cuts at: 3551 3551
Size: 6247
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5254 809
Size: 507 3938 1802
Fragments arranged by size:
3938 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3694 4583 6212
Size: 54 1425 50 275 18 1864 889 1629
Cuts at: 6212 8
Size: 43
Fragments arranged by size:
1864 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3694 1537
Size: 275 1882 4090
Fragments arranged by size:
4090 1882 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2982 3240 3327 4177 1353
Size: 1629 258 87 850 3423
Fragments arranged by size:
3423 1629 850 258 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4834 5208 5354 387
Size: 1064 138 3245 374 146 1280
Fragments arranged by size:
3245 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 2195 2200 2901 3288 3309 4310
Size: 393 462 101 5 701 387 21 1001
Cuts at: 4310 4907 5694 5962 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1001 787 701 597 462 393 387
268 101 21 5
EcoRI G'AATT_C
Cuts at: 3046 3556 3934 3046
Size: 510 378 5359
Fragments arranged by size:
5359 510 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2093 3011 3409 2093
Size: 918 398 4931
Fragments arranged by size:
4931 918 398
FspI TGC'GCA
Cuts at: 4753 4753
Size: 6247
HaeII r_GCGC'y
Cuts at: 1678 3522 5394 1678
Size: 1844 1872 2531
Fragments arranged by size:
2531 1872 1844
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2039 2047 2071 2198
Size: 32 135 32 443 24 8 24 127
Cuts at: 2198 2216 2230 2248 4888 4920 4962 4994 1373
Size: 18 14 18 2640 32 42 32 2626
Fragments arranged by size:
2640 2626 443 135 127 42 32 32
32 32 24 24 18 18 14 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3147 3591 3837 669
Size: 988 281 1209 444 246 3079
Fragments arranged by size:
3079 1209 988 444 281 246
HindIII A'AGCT_T
Cuts at: 748 2993 748
Size: 2245 4002
HpaI GTT'AAC
Cuts at: 3147 3837 3147
Size: 690 5557
KasI G'GCGC_C
Cuts at: 3518 3518
Size: 6247
KpnI G_GTAC'C
Cuts at: 3572 3572
Size: 6247
MfeI C'AATT_G
Cuts at: 3846 3846
Size: 6247
MluI A'CGCG_T
Cuts at: 6123 6123
Size: 6247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5361 5545 1305
Size: 4056 184 2007
Fragments arranged by size:
4056 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4425
Fragments arranged by size:
4425 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2697 2789 4205 4564 4723 518
Size: 648 1531 92 1416 359 159 2042
Fragments arranged by size:
2042 1531 1416 648 359 159 92
MspA1I CmG'CkG
Cuts at: 1606 3440 3663 4340 5488 5733 1606
Size: 1834 223 677 1148 245 2120
Fragments arranged by size:
2120 1834 1148 677 245 223
NarI GG'CG_CC
Cuts at: 3519 3519
Size: 6247
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4756
Fragments arranged by size:
4756 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6247
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6247
NotI GC'GGCC_GC
Cuts at: 3694 3694
Size: 6247
NspI r_CATG'y
Cuts at: 1132 1229 2856 2950 3605 5150 1132
Size: 97 1627 94 655 1545 2229
Fragments arranged by size:
2229 1627 1545 655 97 94
PasI CC'CwG_GG
Cuts at: 1348 3294 1348
Size: 1946 4301
PciI A'CATG_T
Cuts at: 2852 2946 5146 2852
Size: 94 2200 3953
Fragments arranged by size:
3953 2200 94
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6247
PfoI T'CCnGG_A
Cuts at: 2216 2216
Size: 6247
PmeI GTTT'AAAC
Cuts at: 3551 3551
Size: 6247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4316 4348 5829 5861 4316
Size: 32 1481 32 4702
Fragments arranged by size:
4702 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6247
PsiI TTA'TAA
Cuts at: 3817 3817
Size: 6247
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2514 2546 2514
Size: 32 6215
PstI C_TGCA'G
Cuts at: 830 2847 3599 830
Size: 2017 752 3478
Fragments arranged by size:
3478 2017 752
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6247
PvuII CAG'CTG
Cuts at: 3440 3440
Size: 6247
SalI G'TCGA_C
Cuts at: 1936 3589 1936
Size: 1653 4594
SbfI CC_TGCA'GG
Cuts at: 3599 3599
Size: 6247
ScaI AGT'ACT
Cuts at: 4495 6207 4495
Size: 1712 4535
SfcI C'TryA_G
Cuts at: 826 1045 2679 2843 2902 3595 4730 5411 5602
Size: 219 1634 164 59 693 1135 681 191
Cuts at: 5602 826
Size: 1471
Fragments arranged by size:
1634 1471 1135 693 681 219 191 164
59
SfoI GGC'GCC
Cuts at: 3520 3520
Size: 6247
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6247
SmaI CCC'GGG
Cuts at: 1872 3574 1872
Size: 1702 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2233 4351 5252 5514 820
Size: 197 926 290 2118 901 262 1553
Fragments arranged by size:
2118 1553 926 901 290 262 197
SnaBI TAC'GTA
Cuts at: 493 4038 493
Size: 3545 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6247
SphI G_CATG'C
Cuts at: 1132 3605 1132
Size: 2473 3774
SspI AAT'ATT
Cuts at: 5 52 2367 4171 5
Size: 47 2315 1804 2081
Fragments arranged by size:
2315 2081 1804 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6247
StyI C'CwwG_G
Cuts at: 513 1065 2004 3484 3686 513
Size: 552 939 1480 202 3074
Fragments arranged by size:
3074 1480 939 552 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4274 4291 4444 4629 5074 325
Size: 1255 2694 17 153 185 445 1498
Fragments arranged by size:
2694 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2074 2707 2943
Size: 274 80 33 51 1443 97 633 236
Cuts at: 2943 4493 6205 96
Size: 1550 1712 138
Fragments arranged by size:
1712 1550 1443 633 274 236 138 97
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6207 6239 6207
Size: 32 6215
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6247
XbaI T'CTAG_A
Cuts at: 3583 3583
Size: 6247
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6247
XmaI C'CCGG_G
Cuts at: 1870 3572 1870
Size: 1702 4545
XmnI GAAnn'nnTTC
Cuts at: 4376 4376
Size: 6247
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4055 276
Size: 53 83 186 936 2521 2468
Fragments arranged by size:
2521 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI