Restriction map for
pFN21AE0769
(Circular) (Six-base) MAPSORT of: pFN21AE0769.seq Check: 411 from: 1 to: 5722
pFN21AE0769 5722 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2166 2832 2166
Size: 666 5056
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3532 278
Size: 53 83 186 936 1996 2468
Fragments arranged by size:
2468 1996 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3065 1937
Size: 1128 4594
Acc65I G'GTAC_C
Cuts at: 3043 3043
Size: 5722
AclI AA'CG_TT
Cuts at: 1563 3849 4222 1563
Size: 2286 373 3063
Fragments arranged by size:
3063 2286 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2279 3785 5169 5437 1632
Size: 647 1506 1384 268 1917
Fragments arranged by size:
1917 1506 1384 647 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5525
AflIII A'CryG_T
Cuts at: 1203 4621 5598 1203
Size: 3418 977 1327
Fragments arranged by size:
3418 1327 977
AgeI A'CCGG_T
Cuts at: 4553 4553
Size: 5722
AhdI GACnn_n'nnGTC
Cuts at: 4451 4451
Size: 5722
AleI CACnn'nnGTG
Cuts at: 1166 2453 1166
Size: 1287 4435
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5722
AlwNI CAG_nnn'CTG
Cuts at: 2152 2664 3016 3465 5037 2152
Size: 512 352 449 1572 2837
Fragments arranged by size:
2837 1572 512 449 352
ApaI G_GGCC'C
Cuts at: 2241 2949 2241
Size: 708 5014
ApaLI G'TGCA_C
Cuts at: 3779 4935 3779
Size: 1156 4566
ApoI r'AATT_y
Cuts at: 1493 3031 3257 3409 5518 1493
Size: 1538 226 152 2109 1697
Fragments arranged by size:
2109 1697 1538 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4276 160
Size: 872 3244 1606
Fragments arranged by size:
3244 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
AvaI C'yCGr_G
Cuts at: 1870 1943 2491 2541 2577 3047 1870
Size: 73 548 50 36 470 4545
Fragments arranged by size:
4545 548 470 73 50 36
BamHI G'GATC_C
Cuts at: 3052 3419 3052
Size: 367 5355
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2043 2251 2773 3043 4498
Size: 325 217 627 256 208 522 270 1455
Cuts at: 4498 618
Size: 1842
Fragments arranged by size:
1842 1455 627 522 325 270 256 217
208
BanII G_rGCy'C
Cuts at: 1391 2093 2229 2241 2298 2949 3041 1391
Size: 702 136 12 57 651 92 4072
Fragments arranged by size:
4072 702 651 136 92 57 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2779 928
Size: 971 880 3871
Fragments arranged by size:
3871 971 880
BbvCI CC'TCA_GC
Cuts at: 2981 2981
Size: 5722
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3913 3947 1903
Size: 34 1976 34 3678
Fragments arranged by size:
3678 1976 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3614 4824 3614
Size: 1210 4512
BclI T'GATC_A
Cuts at: 1548 2308 1548
Size: 760 4962
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2072 4333 136
Size: 107 122 71 631 605 400 2261 1525
Fragments arranged by size:
2261 1525 631 605 400 122 107 71
BglII A'GATC_T
Cuts at: 5717 5717
Size: 5722
BlpI GC'TnA_GC
Cuts at: 3139 3139
Size: 5722
Bme1580I G_kGCm'C
Cuts at: 1790 2048 2218 2241 2256 2413 2671 2778 2949
Size: 258 170 23 15 157 258 107 171
Cuts at: 2949 3783 4939 1790
Size: 834 1156 2573
Fragments arranged by size:
2573 1156 834 258 258 171 170 157
107 23 15
BmgBI CAC'GTC
Cuts at: 1318 2692 1318
Size: 1374 4348
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3453 4411 442
Size: 416 53 83 399 24 2036 958 1753
Fragments arranged by size:
2036 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2733 1056
Size: 1677 4045
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2641 2867 4382 1239
Size: 1402 226 1515 2579
Fragments arranged by size:
2579 1515 1402 226
Bpu10I CC'TnA_GC
Cuts at: 2718 2981 2718
Size: 263 5459
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3847 4712 5010 3847
Size: 865 298 4559
Fragments arranged by size:
4559 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2344 2513 4385 882
Size: 617 845 169 1872 2219
Fragments arranged by size:
2219 1872 845 617 169
BsaAI yAC'GTr
Cuts at: 493 1204 3513 493
Size: 711 2309 2702
Fragments arranged by size:
2702 2309 711
BsaBI GATnn'nnATC
Cuts at: 5716 5716
Size: 5722
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3529 3911 4546 275
Size: 53 83 186 936 1996 382 635 1451
Fragments arranged by size:
1996 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2790 4155 4553 4827 4974 5385 2790
Size: 1365 398 274 147 411 3127
Fragments arranged by size:
3127 1365 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5497
Fragments arranged by size:
5497 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2222 2945 1186
Size: 1036 723 3963
Fragments arranged by size:
3963 1036 723
BseYI C'CCAG_C
Cuts at: 2092 2523 2695 2722 2840 4925 5421 2092
Size: 431 172 27 118 2085 496 2393
Fragments arranged by size:
2393 2085 496 431 172 118 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2138 2494 1163
Size: 573 402 356 4391
Fragments arranged by size:
4391 573 402 356
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2305 3172 3933 4961 1540
Size: 275 186 304 867 761 1028 2301
Fragments arranged by size:
2301 1028 867 761 304 275 186
BsiHKAI G_wGCw'C
Cuts at: 2823 3783 3868 4939 2823
Size: 960 85 1071 3606
Fragments arranged by size:
3606 1071 960 85
BsmI GAATG_Cn'
Cuts at: 3233 3326 3233
Size: 93 5629
BsmBI CGTCTCn'nnnn_
Cuts at: 2682 2682
Size: 5722
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2093 2218 2229 2241 2256 2298
Size: 399 258 45 125 11 12 15 42
Cuts at: 2298 2413 2671 2778 2823 2949 3041 3783 3868
Size: 115 258 107 45 126 92 742 85
Cuts at: 3868 4939 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 258 258 126 125
115 107 92 85 45 45 42 15
12 11
BspEI T'CCGG_A
Cuts at: 2790 5385 2790
Size: 2595 3127
BspHI T'CATG_A
Cuts at: 3611 5341 3611
Size: 1730 3992
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2166 2832 3077 844
Size: 1322 666 245 3489
Fragments arranged by size:
3489 1322 666 245
BsrBI CCG'CTC
Cuts at: 1394 1823 2172 2212 2490 3609 1394
Size: 429 349 40 278 1119 3507
Fragments arranged by size:
3507 1119 429 349 278 40
BsrDI GCAATG_nn'
Cuts at: 65 1248 4217 4391 65
Size: 1183 2969 174 1396
Fragments arranged by size:
2969 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2827 4366 4553 1700
Size: 110 1017 1539 187 2869
Fragments arranged by size:
2869 1539 1017 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5722
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5722
BssSI C'ACGA_G
Cuts at: 2596 3782 4794 2596
Size: 1186 1012 3524
Fragments arranged by size:
3524 1186 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2161 2763 794
Size: 1367 602 3753
Fragments arranged by size:
3753 1367 602
BstBI TT'CG_AA
Cuts at: 4526 4526
Size: 5722
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5722
BstYI r'GATC_y
Cuts at: 1972 2148 3052 3419 3804 3821 5273 5359 5371
Size: 176 904 367 385 17 1452 86 12
Cuts at: 5371 5717 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 904 385 367 346 176 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2018 2205 2018
Size: 187 5535
BtgI C'CryG_G
Cuts at: 513 1065 2004 2084 2503 513
Size: 552 939 80 419 3732
Fragments arranged by size:
3732 939 552 419 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4983
Fragments arranged by size:
4983 683 56
BtsI GCAGTG_nn'
Cuts at: 2383 2768 3238 4032 4052 2383
Size: 385 470 794 20 4053
Fragments arranged by size:
4053 794 470 385 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5722
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5687
DraI TTT'AAA
Cuts at: 3026 3026
Size: 5722
DraIII CAC_nnn'GTG
Cuts at: 2043 2763 2043
Size: 720 5002
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4729 809
Size: 507 3413 1802
Fragments arranged by size:
3413 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2302 2590 2734
Size: 54 1425 50 275 18 472 288 144
Cuts at: 2734 3169 4058 5687 8
Size: 435 889 1629 43
Fragments arranged by size:
1629 1425 889 472 435 288 275 144
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2302 3169 1537
Size: 275 490 867 4090
Fragments arranged by size:
4090 867 490 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2111 2894 3652 1353
Size: 758 783 758 3423
Fragments arranged by size:
3423 783 758 758
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4309 4683 4829 387
Size: 1064 138 2720 374 146 1280
Fragments arranged by size:
2720 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2279 2641 2867 3785 4382 5169 5437
Size: 393 647 362 226 918 597 787 268
Cuts at: 5437 1239
Size: 1524
Fragments arranged by size:
1524 918 787 647 597 393 362 268
226
EcoO109I rG'GnC_Cy
Cuts at: 2237 2281 2496 2582 2661 2700 2946 3013 2237
Size: 44 215 86 79 39 246 67 4946
Fragments arranged by size:
4946 246 215 86 79 67 44 39
EcoRI G'AATT_C
Cuts at: 3031 3409 3031
Size: 378 5344
FspI TGC'GCA
Cuts at: 2459 2756 4228 2459
Size: 297 1472 3953
Fragments arranged by size:
3953 1472 297
FspAI rTGC'GCAy
Cuts at: 2756 2756
Size: 5722
HaeII r_GCGC'y
Cuts at: 1678 4869 1678
Size: 3191 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2300 2332 4363 4395 4437
Size: 32 135 32 728 32 2031 32 42
Cuts at: 4437 4469 1373
Size: 32 2626
Fragments arranged by size:
2626 2031 728 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3066 3312 669
Size: 988 281 1128 246 3079
Fragments arranged by size:
3079 1128 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5722
HpaI GTT'AAC
Cuts at: 3312 3312
Size: 5722
KpnI G_GTAC'C
Cuts at: 3047 3047
Size: 5722
MfeI C'AATT_G
Cuts at: 3321 3321
Size: 5722
MluI A'CGCG_T
Cuts at: 5598 5598
Size: 5722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2379 4836 5020 1305
Size: 1074 2457 184 2007
Fragments arranged by size:
2457 2007 1074 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2592 10
Size: 54 1425 343 760 3140
Fragments arranged by size:
3140 1425 760 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2453 3680 4039 4198 518
Size: 648 1287 1227 359 159 2042
Fragments arranged by size:
2042 1287 1227 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2182 2505 2527 2533 3138 3815 4963 5208
Size: 576 323 22 6 605 677 1148 245
Cuts at: 5208 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 605 576 323 245 22
6
NaeI GCC'GGC
Cuts at: 2829 2829
Size: 5722
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4231
Fragments arranged by size:
4231 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5722
NgoMIV G'CCGG_C
Cuts at: 2827 2827
Size: 5722
NheI G'CTAG_C
Cuts at: 1052 2729 1052
Size: 1677 4045
NotI GC'GGCC_GC
Cuts at: 3169 3169
Size: 5722
NspI r_CATG'y
Cuts at: 1132 1229 3080 4625 1132
Size: 97 1851 1545 2229
Fragments arranged by size:
2229 1851 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2234 1348
Size: 886 4836
PciI A'CATG_T
Cuts at: 4621 4621
Size: 5722
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2335 1108
Size: 1227 4495
PmeI GTTT'AAAC
Cuts at: 3026 3026
Size: 5722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2871 2903 3791 3823 5304 5336 2871
Size: 32 888 32 1481 32 3257
Fragments arranged by size:
3257 1481 888 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2582 2582
Size: 5722
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5722
PsiI TTA'TAA
Cuts at: 3292 3292
Size: 5722
PspOMI G'GGCC_C
Cuts at: 2237 2945 2237
Size: 708 5014
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2909 2941 2909
Size: 32 5690
PstI C_TGCA'G
Cuts at: 830 2532 3012 3074 830
Size: 1702 480 62 3478
Fragments arranged by size:
3478 1702 480 62
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5722
PvuII CAG'CTG
Cuts at: 2182 2527 2533 2182
Size: 345 6 5371
Fragments arranged by size:
5371 345 6
SacII CC_GC'GG
Cuts at: 2506 2506
Size: 5722
SalI G'TCGA_C
Cuts at: 1936 3064 1936
Size: 1128 4594
SapI GCTCTTCn'nnn_
Cuts at: 2111 2111
Size: 5722
SbfI CC_TGCA'GG
Cuts at: 3074 3074
Size: 5722
ScaI AGT'ACT
Cuts at: 3970 5682 3970
Size: 1712 4010
SexAI A'CCwGG_T
Cuts at: 2039 2781 2039
Size: 742 4980
SfcI C'TryA_G
Cuts at: 826 1045 2528 3008 3070 4205 4886 5077 826
Size: 219 1483 480 62 1135 681 191 1471
Fragments arranged by size:
1483 1471 1135 681 480 219 191 62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5722
SmaI CCC'GGG
Cuts at: 1872 2579 3049 1872
Size: 707 470 4545
Fragments arranged by size:
4545 707 470
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3826 4727 4989 820
Size: 197 926 1883 901 262 1553
Fragments arranged by size:
1883 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3513 493
Size: 3020 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5722
SphI G_CATG'C
Cuts at: 1132 3080 1132
Size: 1948 3774
SspI AAT'ATT
Cuts at: 5 52 3646 5
Size: 47 3594 2081
Fragments arranged by size:
3594 2081 47
StuI AGG'CCT
Cuts at: 1517 2549 1517
Size: 1032 4690
StyI C'CwwG_G
Cuts at: 513 1065 2004 3161 513
Size: 552 939 1157 3074
Fragments arranged by size:
3074 1157 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2714 3749 3766 3919 4104 4549 325
Size: 1255 1134 1035 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1134 1035 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3968 5680 96
Size: 274 80 33 51 1443 1991 1712 138
Fragments arranged by size:
1991 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5682 5714 5682
Size: 32 5690
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5722
XbaI T'CTAG_A
Cuts at: 3058 3058
Size: 5722
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5722
XmaI C'CCGG_G
Cuts at: 1870 2577 3047 1870
Size: 707 470 4545
Fragments arranged by size:
4545 707 470
XmnI GAAnn'nnTTC
Cuts at: 3851 3851
Size: 5722
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3530 276
Size: 53 83 186 936 1996 2468
Fragments arranged by size:
2468 1996 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FseI
KasI NarI NruI NsiI PacI PfoI PmlI PspXI
RsrII SacI SanDI SfiI SfoI SrfI SwaI XcmI