Restriction map for
pFN21AE0762
(Circular) (Six-base) MAPSORT of: pFN21AE0762.seq Check: 8678 from: 1 to: 6013
pFN21AE0762 6013 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3823 278
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3270 3356 1937
Size: 1333 86 4594
Fragments arranged by size:
4594 1333 86
Acc65I G'GTAC_C
Cuts at: 3334 3334
Size: 6013
AclI AA'CG_TT
Cuts at: 1563 4140 4513 1563
Size: 2577 373 3063
Fragments arranged by size:
3063 2577 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2906 4076 5460 5728 1632
Size: 1274 1170 1384 268 1917
Fragments arranged by size:
1917 1384 1274 1170 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5816
AflIII A'CryG_T
Cuts at: 1203 2166 2883 4912 5889 1203
Size: 963 717 2029 977 1327
Fragments arranged by size:
2029 1327 977 963 717
AgeI A'CCGG_T
Cuts at: 4844 4844
Size: 6013
AhdI GACnn_n'nnGTC
Cuts at: 4742 4742
Size: 6013
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6013
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6013
AlwNI CAG_nnn'CTG
Cuts at: 3756 5328 3756
Size: 1572 4441
ApaLI G'TGCA_C
Cuts at: 4070 5226 4070
Size: 1156 4857
ApoI r'AATT_y
Cuts at: 1493 2229 3296 3322 3548 3700 5809 1493
Size: 736 1067 26 226 152 2109 1697
Fragments arranged by size:
2109 1697 1067 736 226 152 26
AseI AT'TA_AT
Cuts at: 160 1032 4567 160
Size: 872 3535 1606
Fragments arranged by size:
3535 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
AvaI C'yCGr_G
Cuts at: 1870 1943 2361 2855 3338 1870
Size: 73 418 494 483 4545
Fragments arranged by size:
4545 494 483 418 73
BamHI G'GATC_C
Cuts at: 3022 3343 3710 3022
Size: 321 367 5325
Fragments arranged by size:
5325 367 321
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2804 2986 3334 4789 618
Size: 325 217 627 1017 182 348 1455 1842
Fragments arranged by size:
1842 1455 1017 627 348 325 217 182
BanII G_rGCy'C
Cuts at: 1391 2478 2895 3332 1391
Size: 1087 417 437 4072
Fragments arranged by size:
4072 1087 437 417
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5042
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4204 4238 1903
Size: 34 2267 34 3678
Fragments arranged by size:
3678 2267 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3905 5115 3905
Size: 1210 4803
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6013
BfrBI ATG'CAT
Cuts at: 2566 2566
Size: 6013
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4624 136
Size: 107 122 71 631 605 2952 1525
Fragments arranged by size:
2952 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2590 6008 2590
Size: 3418 2595
BlpI GC'TnA_GC
Cuts at: 3430 3430
Size: 6013
Bme1580I G_kGCm'C
Cuts at: 1790 2864 4074 5230 1790
Size: 1074 1210 1156 2573
Fragments arranged by size:
2573 1210 1156 1074
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6013
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3209 3744 4702
Size: 416 53 83 399 24 1792 535 958
Cuts at: 4702 442
Size: 1753
Fragments arranged by size:
1792 1753 958 535 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6013
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3156 3156
Size: 6013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2356 4673 1239
Size: 1117 2317 2579
Fragments arranged by size:
2579 2317 1117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3038 4138 5003 5301 3038
Size: 1100 865 298 3750
Fragments arranged by size:
3750 1100 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2420 2706 3240 4676 882
Size: 617 921 286 534 1436 2219
Fragments arranged by size:
2219 1436 921 617 534 286
BsaAI yAC'GTr
Cuts at: 493 1204 2758 2884 3804 493
Size: 711 1554 126 920 2702
Fragments arranged by size:
2702 1554 920 711 126
BsaBI GATnn'nnATC
Cuts at: 6007 6007
Size: 6013
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2141 3820 4202 4837
Size: 53 83 186 936 608 1679 382 635
Cuts at: 4837 275
Size: 1451
Fragments arranged by size:
1679 1451 936 635 608 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4446 4844 5118 5265 5676 4446
Size: 398 274 147 411 4783
Fragments arranged by size:
4783 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5788
Fragments arranged by size:
5788 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 2694 2928 1186
Size: 946 562 234 4271
Fragments arranged by size:
4271 946 562 234
BseYI C'CCAG_C
Cuts at: 2203 5216 5712 2203
Size: 3013 496 2504
Fragments arranged by size:
3013 2504 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2372 2426 1163
Size: 573 636 54 4750
Fragments arranged by size:
4750 636 573 54
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3463 4224 5252 1540
Size: 275 186 1462 761 1028 2301
Fragments arranged by size:
2301 1462 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2478 2742 4074 4159 5230 2478
Size: 264 1332 85 1071 3261
Fragments arranged by size:
3261 1332 1071 264 85
BsmI GAATG_Cn'
Cuts at: 3524 3617 3524
Size: 93 5920
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2478 2742 2864 2895 3332 4074 4159
Size: 399 688 264 122 31 437 742 85
Cuts at: 4159 5230 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 688 437 399 264 122
85 31
BspEI T'CCGG_A
Cuts at: 5676 5676
Size: 6013
BspHI T'CATG_A
Cuts at: 2961 3219 3902 5632 2961
Size: 258 683 1730 3342
Fragments arranged by size:
3342 1730 683 258
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3368 844
Size: 2524 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3900 1394
Size: 429 2077 3507
Fragments arranged by size:
3507 2077 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4508 4682 65
Size: 1183 3260 174 1396
Fragments arranged by size:
3260 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4657 4844 1700
Size: 110 2847 187 2869
Fragments arranged by size:
2869 2847 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6013
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6013
BssSI C'ACGA_G
Cuts at: 4073 5085 4073
Size: 1012 5001
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2986 794
Size: 2192 3821
BstBI TT'CG_AA
Cuts at: 3118 4817 3118
Size: 1699 4314
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6013
BstYI r'GATC_y
Cuts at: 1972 2313 2590 2928 3022 3343 3710 4095 4112
Size: 341 277 338 94 321 367 385 17
Cuts at: 4112 5564 5650 5662 6008 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 341 338 321
277 94 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2829 2934 513
Size: 552 1764 105 3592
Fragments arranged by size:
3592 1764 552 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5274
Fragments arranged by size:
5274 683 56
BtsI GCAGTG_nn'
Cuts at: 2019 3529 4323 4343 2019
Size: 1510 794 20 3689
Fragments arranged by size:
3689 1510 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6013
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5978
DraI TTT'AAA
Cuts at: 3317 3317
Size: 6013
DraIII CAC_nnn'GTG
Cuts at: 2449 2449
Size: 6013
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5020 809
Size: 507 3704 1802
Fragments arranged by size:
3704 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2304 2832 3460
Size: 54 1425 50 275 18 474 528 628
Cuts at: 3460 4349 5978 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 628 528 474 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3460 1537
Size: 275 1648 4090
Fragments arranged by size:
4090 1648 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2483 2732 3102 3943 1353
Size: 1130 249 370 841 3423
Fragments arranged by size:
3423 1130 841 370 249
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2396 4600 4974 5120 387
Size: 1064 138 807 2204 374 146 1280
Fragments arranged by size:
2204 1280 1064 807 374 146 138
EcoICRI GAG'CTC
Cuts at: 2476 2476
Size: 6013
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2356 2906 4076 4673 5460 5728 1239
Size: 393 724 550 1170 597 787 268 1524
Fragments arranged by size:
1524 1170 787 724 597 550 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3261 3261
Size: 6013
EcoRI G'AATT_C
Cuts at: 2229 3322 3700 2229
Size: 1093 378 4542
Fragments arranged by size:
4542 1093 378
EcoRV GAT'ATC
Cuts at: 2764 2764
Size: 6013
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2288 2461 2548 2288
Size: 173 87 5753
Fragments arranged by size:
5753 173 87
FspI TGC'GCA
Cuts at: 4519 4519
Size: 6013
HaeII r_GCGC'y
Cuts at: 1678 5160 1678
Size: 3482 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2226 2258 2417 2449 4654
Size: 32 135 32 654 32 159 32 2205
Cuts at: 4654 4686 4728 4760 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2205 654 159 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3357 3603 669
Size: 988 281 1419 246 3079
Fragments arranged by size:
3079 1419 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2270 748
Size: 1522 4491
HpaI GTT'AAC
Cuts at: 3603 3603
Size: 6013
KpnI G_GTAC'C
Cuts at: 3338 3338
Size: 6013
MfeI C'AATT_G
Cuts at: 3612 3612
Size: 6013
MluI A'CGCG_T
Cuts at: 5889 5889
Size: 6013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3018 3040 5127 5311 1305
Size: 1713 22 2087 184 2007
Fragments arranged by size:
2087 2007 1713 184 22
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2306 2834 10
Size: 54 1425 343 474 528 3189
Fragments arranged by size:
3189 1425 528 474 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2565 2756 3971 4330 4489 518
Size: 648 1399 191 1215 359 159 2042
Fragments arranged by size:
2042 1399 1215 648 359 191 159
MspA1I CmG'CkG
Cuts at: 1606 2137 3429 4106 5254 5499 1606
Size: 531 1292 677 1148 245 2120
Fragments arranged by size:
2120 1292 1148 677 531 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5461
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6013
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6013
NotI GC'GGCC_GC
Cuts at: 3460 3460
Size: 6013
NsiI A_TGCA'T
Cuts at: 2568 2568
Size: 6013
NspI r_CATG'y
Cuts at: 1132 1229 2170 2566 3371 4916 1132
Size: 97 941 396 805 1545 2229
Fragments arranged by size:
2229 1545 941 805 396 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6013
PciI A'CATG_T
Cuts at: 2166 4912 2166
Size: 2746 3267
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6013
PfoI T'CCnGG_A
Cuts at: 2316 2730 2316
Size: 414 5599
PmeI GTTT'AAAC
Cuts at: 3317 3317
Size: 6013
PmlI CAC'GTG
Cuts at: 2884 2884
Size: 6013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4082 4114 5595 5627 4082
Size: 32 1481 32 4468
Fragments arranged by size:
4468 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3261 3261
Size: 6013
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6013
PsiI TTA'TAA
Cuts at: 3583 3583
Size: 6013
PspXI vC'TCGA_Gb
Cuts at: 2855 2855
Size: 6013
PstI C_TGCA'G
Cuts at: 830 2014 3365 830
Size: 1184 1351 3478
Fragments arranged by size:
3478 1351 1184
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6013
PvuII CAG'CTG
Cuts at: 2137 2137
Size: 6013
SacI G_AGCT'C
Cuts at: 2478 2478
Size: 6013
SalI G'TCGA_C
Cuts at: 1936 3355 1936
Size: 1419 4594
SanDI GG'GwC_CC
Cuts at: 3261 3261
Size: 6013
SapI GCTCTTCn'nnn_
Cuts at: 2483 3102 2483
Size: 619 5394
SbfI CC_TGCA'GG
Cuts at: 3365 3365
Size: 6013
ScaI AGT'ACT
Cuts at: 4261 5973 4261
Size: 1712 4301
SfcI C'TryA_G
Cuts at: 826 1045 2010 3163 3271 3361 4496 5177 5368
Size: 219 965 1153 108 90 1135 681 191
Cuts at: 5368 826
Size: 1471
Fragments arranged by size:
1471 1153 1135 965 681 219 191 108
90
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6013
SmaI CCC'GGG
Cuts at: 1872 3340 1872
Size: 1468 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2855 3053 4117 5018 5280 820
Size: 197 926 912 198 1064 901 262 1553
Fragments arranged by size:
1553 1064 926 912 901 262 198 197
SnaBI TAC'GTA
Cuts at: 493 3804 493
Size: 3311 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6013
SphI G_CATG'C
Cuts at: 1132 3371 1132
Size: 2239 3774
SspI AAT'ATT
Cuts at: 5 52 3937 5
Size: 47 3885 2081
Fragments arranged by size:
3885 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6013
StyI C'CwwG_G
Cuts at: 513 1065 2772 3127 3452 513
Size: 552 1707 355 325 3074
Fragments arranged by size:
3074 1707 552 355 325
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2111 4040 4057 4210 4395 4840 325
Size: 1255 531 1929 17 153 185 445 1498
Fragments arranged by size:
1929 1498 1255 531 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2787 4259 5971
Size: 274 80 33 51 1443 810 1472 1712
Cuts at: 5971 96
Size: 138
Fragments arranged by size:
1712 1472 1443 810 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5973 6005 5973
Size: 32 5981
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6013
XbaI T'CTAG_A
Cuts at: 3349 3349
Size: 6013
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2270 2299 2314 2443 2270
Size: 29 15 129 5840
Fragments arranged by size:
5840 129 29 15
XhoI C'TCGA_G
Cuts at: 1943 2855 1943
Size: 912 5101
XmaI C'CCGG_G
Cuts at: 1870 3338 1870
Size: 1468 4545
XmnI GAAnn'nnTTC
Cuts at: 2690 4142 2690
Size: 1452 4561
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3821 276
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PspOMI PsrI RsrII SacII SexAI SfiI SfoI SrfI
SwaI