Restriction map for
pFN21AE0761
(Circular) (Six-base) MAPSORT of: pFN21AE0761.seq Check: 9563 from: 1 to: 5716
pFN21AE0761 5716 bp
With 182 enzymes: SgfI *
October 11, 2011 14:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2085 2472 2563 2085
Size: 387 91 5238
Fragments arranged by size:
5238 387 91
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3526 278
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3059 1937
Size: 1122 4594
Acc65I G'GTAC_C
Cuts at: 3037 3037
Size: 5716
AclI AA'CG_TT
Cuts at: 1563 3843 4216 1563
Size: 2280 373 3063
Fragments arranged by size:
3063 2280 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2989 3779 5163 5431 1632
Size: 1357 790 1384 268 1917
Fragments arranged by size:
1917 1384 1357 790 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5519
AflIII A'CryG_T
Cuts at: 1203 4615 5592 1203
Size: 3412 977 1327
Fragments arranged by size:
3412 1327 977
AgeI A'CCGG_T
Cuts at: 4547 4547
Size: 5716
AhdI GACnn_n'nnGTC
Cuts at: 4445 4445
Size: 5716
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5716
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5716
AlwNI CAG_nnn'CTG
Cuts at: 2871 3459 5031 2871
Size: 588 1572 3556
Fragments arranged by size:
3556 1572 588
ApaI G_GGCC'C
Cuts at: 2433 2433
Size: 5716
ApaLI G'TGCA_C
Cuts at: 3773 4929 3773
Size: 1156 4560
ApoI r'AATT_y
Cuts at: 1493 3025 3251 3403 5512 1493
Size: 1532 226 152 2109 1697
Fragments arranged by size:
2109 1697 1532 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4270 160
Size: 872 3238 1606
Fragments arranged by size:
3238 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
AvaI C'yCGr_G
Cuts at: 1870 1943 2545 2693 2943 3041 1870
Size: 73 602 148 250 98 4545
Fragments arranged by size:
4545 602 250 148 98 73
BamHI G'GATC_C
Cuts at: 3046 3413 3046
Size: 367 5349
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3037 4492 618
Size: 325 217 627 1250 1455 1842
Fragments arranged by size:
1842 1455 1250 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2292 2340 2433 2552 2915 3035 1391
Size: 901 48 93 119 363 120 4072
Fragments arranged by size:
4072 901 363 120 119 93 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4745
BbvCI CC'TCA_GC
Cuts at: 2634 2634
Size: 5716
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3907 3941 1903
Size: 34 1970 34 3678
Fragments arranged by size:
3678 1970 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3608 4818 3608
Size: 1210 4506
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5716
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2026 4327 136
Size: 107 122 71 631 605 354 2301 1525
Fragments arranged by size:
2301 1525 631 605 354 122 107 71
BglII A'GATC_T
Cuts at: 5711 5711
Size: 5716
BlpI GC'TnA_GC
Cuts at: 3133 3133
Size: 5716
Bme1580I G_kGCm'C
Cuts at: 1790 2383 2433 3777 4933 1790
Size: 593 50 1344 1156 2573
Fragments arranged by size:
2573 1344 1156 593 50
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5716
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2386 3447 4405
Size: 416 53 83 399 24 969 1061 958
Cuts at: 4405 442
Size: 1753
Fragments arranged by size:
1753 1061 969 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4376 1239
Size: 3137 2579
Bpu10I CC'TnA_GC
Cuts at: 2634 2909 2634
Size: 275 5441
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3841 4706 5004 3841
Size: 865 298 4553
Fragments arranged by size:
4553 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4379 882
Size: 617 2880 2219
Fragments arranged by size:
2880 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2746 3507 493
Size: 711 1542 761 2702
Fragments arranged by size:
2702 1542 761 711
BsaBI GATnn'nnATC
Cuts at: 5710 5710
Size: 5716
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2187 3523 3905 4540
Size: 53 83 186 936 654 1336 382 635
Cuts at: 4540 275
Size: 1451
Fragments arranged by size:
1451 1336 936 654 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4149 4547 4821 4968 5379 4149
Size: 398 274 147 411 4486
Fragments arranged by size:
4486 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5491
Fragments arranged by size:
5491 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2300 2333 2753 1186
Size: 1114 33 420 4149
Fragments arranged by size:
4149 1114 420 33
BseYI C'CCAG_C
Cuts at: 2159 2328 2793 4919 5415 2159
Size: 169 465 2126 496 2460
Fragments arranged by size:
2460 2126 496 465 169
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2063 1163
Size: 573 327 4816
Fragments arranged by size:
4816 573 327
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2030 2925 3166 3927 4955 1540
Size: 275 186 29 895 241 761 1028 2301
Fragments arranged by size:
2301 1028 895 761 275 241 186 29
BsiHKAI G_wGCw'C
Cuts at: 2292 2745 2819 3777 3862 4933 2292
Size: 453 74 958 85 1071 3075
Fragments arranged by size:
3075 1071 958 453 85 74
BsmI GAATG_Cn'
Cuts at: 3227 3320 3227
Size: 93 5623
BsmBI CGTCTCn'nnnn_
Cuts at: 2198 2198
Size: 5716
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2292 2340 2383 2433 2552 2745 2819
Size: 399 502 48 43 50 119 193 74
Cuts at: 2819 2915 3035 3777 3862 4933 1391
Size: 96 120 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 502 399 193 120 119
96 85 74 50 48 43
BspEI T'CCGG_A
Cuts at: 5379 5379
Size: 5716
BspHI T'CATG_A
Cuts at: 3605 5335 3605
Size: 1730 3986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2085 2472 2505 2563 2841 3071 844
Size: 1241 387 33 58 278 230 3489
Fragments arranged by size:
3489 1241 387 278 230 58 33
BsrBI CCG'CTC
Cuts at: 1394 1823 2535 2565 2898 3603 1394
Size: 429 712 30 333 705 3507
Fragments arranged by size:
3507 712 705 429 333 30
BsrDI GCAATG_nn'
Cuts at: 65 1248 4211 4385 65
Size: 1183 2963 174 1396
Fragments arranged by size:
2963 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2295 2387 2586 4360 4547 1700
Size: 110 485 92 199 1774 187 2869
Fragments arranged by size:
2869 1774 485 199 187 110 92
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5716
BssHII G'CGCG_C
Cuts at: 1925 2155 2965 2967 1925
Size: 230 810 2 4674
Fragments arranged by size:
4674 810 230 2
BssSI C'ACGA_G
Cuts at: 3776 4788 3776
Size: 1012 4704
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5716
BstBI TT'CG_AA
Cuts at: 4520 4520
Size: 5716
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5716
BstXI CCAn_nnnn'nTGG
Cuts at: 2801 2801
Size: 5716
BstYI r'GATC_y
Cuts at: 1972 2881 3046 3413 3798 3815 5267 5353 5365
Size: 909 165 367 385 17 1452 86 12
Cuts at: 5365 5711 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 909 385 367 346 165 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2435 2478 2435
Size: 43 5673
BtgI C'CryG_G
Cuts at: 513 1065 2004 2098 2916 513
Size: 552 939 94 818 3313
Fragments arranged by size:
3313 939 818 552 94
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4977
Fragments arranged by size:
4977 683 56
BtsI GCAGTG_nn'
Cuts at: 2872 3232 4026 4046 2872
Size: 360 794 20 4542
Fragments arranged by size:
4542 794 360 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5716
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5681
DraI TTT'AAA
Cuts at: 3020 3020
Size: 5716
DraIII CAC_nnn'GTG
Cuts at: 2218 2218
Size: 5716
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2171 4723 809
Size: 507 855 2552 1802
Fragments arranged by size:
2552 1802 855 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2027 2521
Size: 54 1425 50 275 18 177 20 494
Cuts at: 2521 2643 2922 3163 4052 5681 8
Size: 122 279 241 889 1629 43
Fragments arranged by size:
1629 1425 889 494 279 275 241 177
122 54 50 43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 2027 2922 3163 1537
Size: 275 215 895 241 4090
Fragments arranged by size:
4090 895 275 241 215
EarI CTCTTCn'nnn_
Cuts at: 1353 3646 1353
Size: 2293 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2282 4303 4677 4823 387
Size: 1064 138 693 2021 374 146 1280
Fragments arranged by size:
2021 1280 1064 693 374 146 138
EcoICRI GAG'CTC
Cuts at: 2290 2290
Size: 5716
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2989 3779 4376 5163 5431 1239
Size: 393 1357 790 597 787 268 1524
Fragments arranged by size:
1524 1357 790 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2174 2468 2174
Size: 294 5422
EcoO109I rG'GnC_Cy
Cuts at: 2430 2474 2753 2430
Size: 44 279 5393
Fragments arranged by size:
5393 279 44
EcoRI G'AATT_C
Cuts at: 3025 3403 3025
Size: 378 5338
FspI TGC'GCA
Cuts at: 4222 4222
Size: 5716
HaeII r_GCGC'y
Cuts at: 1678 4863 1678
Size: 3185 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2534 2566 4357 4389 4431
Size: 32 135 32 962 32 1791 32 42
Cuts at: 4431 4463 1373
Size: 32 2626
Fragments arranged by size:
2626 1791 962 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2182 2191 2959 3060 3306 669
Size: 988 281 244 9 768 101 246 3079
Fragments arranged by size:
3079 988 768 281 246 244 101 9
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5716
HpaI GTT'AAC
Cuts at: 3306 3306
Size: 5716
KpnI G_GTAC'C
Cuts at: 3041 3041
Size: 5716
MfeI C'AATT_G
Cuts at: 3315 3315
Size: 5716
MluI A'CGCG_T
Cuts at: 5592 5592
Size: 5716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2868 4830 5014 1305
Size: 1563 1962 184 2007
Fragments arranged by size:
2007 1962 1563 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2645 10
Size: 54 1425 343 813 3081
Fragments arranged by size:
3081 1425 813 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2597 3674 4033 4192 518
Size: 648 1431 1077 359 159 2042
Fragments arranged by size:
2042 1431 1077 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2023 2100 2305 3132 3809 4957 5202 1606
Size: 417 77 205 827 677 1148 245 2120
Fragments arranged by size:
2120 1148 827 677 417 245 205 77
NaeI GCC'GGC
Cuts at: 2297 2297
Size: 5716
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4225
Fragments arranged by size:
4225 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5716
NgoMIV G'CCGG_C
Cuts at: 2295 2295
Size: 5716
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5716
NotI GC'GGCC_GC
Cuts at: 2027 3163 2027
Size: 1136 4580
NspI r_CATG'y
Cuts at: 1132 1229 3074 4619 1132
Size: 97 1845 1545 2229
Fragments arranged by size:
2229 1845 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2358 1348
Size: 1010 4706
PciI A'CATG_T
Cuts at: 4615 4615
Size: 5716
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2218 1108
Size: 1110 4606
PfoI T'CCnGG_A
Cuts at: 2889 2889
Size: 5716
PmeI GTTT'AAAC
Cuts at: 3020 3020
Size: 5716
PmlI CAC'GTG
Cuts at: 2746 2746
Size: 5716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3817 5298 5330 3785
Size: 32 1481 32 4171
Fragments arranged by size:
4171 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2474 2753 2474
Size: 279 5437
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5716
PsiI TTA'TAA
Cuts at: 3286 3286
Size: 5716
PspOMI G'GGCC_C
Cuts at: 2429 2429
Size: 5716
PspXI vC'TCGA_Gb
Cuts at: 2943 2943
Size: 5716
PstI C_TGCA'G
Cuts at: 830 3068 830
Size: 2238 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5716
PvuII CAG'CTG
Cuts at: 2305 2305
Size: 5716
SacI G_AGCT'C
Cuts at: 2292 2292
Size: 5716
SacII CC_GC'GG
Cuts at: 2101 2101
Size: 5716
SalI G'TCGA_C
Cuts at: 1936 3058 1936
Size: 1122 4594
SbfI CC_TGCA'GG
Cuts at: 3068 3068
Size: 5716
ScaI AGT'ACT
Cuts at: 3964 5676 3964
Size: 1712 4004
SexAI A'CCwGG_T
Cuts at: 2214 2214
Size: 5716
SfcI C'TryA_G
Cuts at: 826 1045 3064 4199 4880 5071 826
Size: 219 2019 1135 681 191 1471
Fragments arranged by size:
2019 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
SgrAI Cr'CCGG_yG
Cuts at: 1700 2295 1700
Size: 595 5121
SmaI CCC'GGG
Cuts at: 1872 2547 3043 1872
Size: 675 496 4545
Fragments arranged by size:
4545 675 496
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2943 3820 4721 4983 820
Size: 197 926 1000 877 901 262 1553
Fragments arranged by size:
1553 1000 926 901 877 262 197
SnaBI TAC'GTA
Cuts at: 493 3507 493
Size: 3014 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5716
SphI G_CATG'C
Cuts at: 1132 3074 1132
Size: 1942 3774
SspI AAT'ATT
Cuts at: 5 52 3640 5
Size: 47 3588 2081
Fragments arranged by size:
3588 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5716
StyI C'CwwG_G
Cuts at: 513 1065 2004 2931 3155 513
Size: 552 939 927 224 3074
Fragments arranged by size:
3074 939 927 552 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2746 3743 3760 3913 4098 4543 325
Size: 1255 1166 997 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1166 997 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2526 3962 5674
Size: 274 80 33 51 1443 549 1436 1712
Cuts at: 5674 96
Size: 138
Fragments arranged by size:
1712 1443 1436 549 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5676 5708 5676
Size: 32 5684
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5716
XbaI T'CTAG_A
Cuts at: 3052 3052
Size: 5716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2801 2979 2801
Size: 178 5538
XhoI C'TCGA_G
Cuts at: 1943 2943 1943
Size: 1000 4716
XmaI C'CCGG_G
Cuts at: 1870 2545 3041 1870
Size: 675 496 4545
Fragments arranged by size:
4545 675 496
XmnI GAAnn'nnTTC
Cuts at: 3845 3845
Size: 5716
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3524 276
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PsrI RsrII SanDI SapI SfiI
SfoI SrfI SwaI