Restriction map for
pFN21AE0759
(Circular) (Six-base) MAPSORT of: pFN21AE0759.seq Check: 2560 from: 1 to: 5698
pFN21AE0759 5698 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3508 278
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3041 1937
Size: 1104 4594
Acc65I G'GTAC_C
Cuts at: 3019 3019
Size: 5698
AclI AA'CG_TT
Cuts at: 1563 3825 4198 1563
Size: 2262 373 3063
Fragments arranged by size:
3063 2262 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2729 3761 5145 5413 1632
Size: 1097 1032 1384 268 1917
Fragments arranged by size:
1917 1384 1097 1032 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5501
AflIII A'CryG_T
Cuts at: 1203 2424 4597 5574 1203
Size: 1221 2173 977 1327
Fragments arranged by size:
2173 1327 1221 977
AgeI A'CCGG_T
Cuts at: 4529 4529
Size: 5698
AhdI GACnn_n'nnGTC
Cuts at: 4427 4427
Size: 5698
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5698
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5698
AlwNI CAG_nnn'CTG
Cuts at: 3441 5013 3441
Size: 1572 4126
ApaLI G'TGCA_C
Cuts at: 2892 3755 4911 2892
Size: 863 1156 3679
Fragments arranged by size:
3679 1156 863
ApoI r'AATT_y
Cuts at: 1493 2135 3007 3233 3385 5494 1493
Size: 642 872 226 152 2109 1697
Fragments arranged by size:
2109 1697 872 642 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2714 4252 160
Size: 872 1682 1538 1606
Fragments arranged by size:
1682 1606 1538 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
AvaI C'yCGr_G
Cuts at: 1870 1943 2220 3023 1870
Size: 73 277 803 4545
Fragments arranged by size:
4545 803 277 73
BamHI G'GATC_C
Cuts at: 3028 3395 3028
Size: 367 5331
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3019 4474 618
Size: 325 217 627 1232 1455 1842
Fragments arranged by size:
1842 1455 1232 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2376 3017 1391
Size: 985 641 4072
Fragments arranged by size:
4072 985 641
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2279 928
Size: 971 380 4347
Fragments arranged by size:
4347 971 380
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3889 3923 1903
Size: 34 1952 34 3678
Fragments arranged by size:
3678 1952 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3590 4800 3590
Size: 1210 4488
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4309 136
Size: 107 122 71 631 605 2637 1525
Fragments arranged by size:
2637 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5693 5693
Size: 5698
BlpI GC'TnA_GC
Cuts at: 2697 3115 2697
Size: 418 5280
Bme1580I G_kGCm'C
Cuts at: 1790 2896 3759 4915 1790
Size: 1106 863 1156 2573
Fragments arranged by size:
2573 1156 1106 863
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5698
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3429 4387 442
Size: 416 53 83 399 24 2012 958 1753
Fragments arranged by size:
2012 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2318 2968 4358 1239
Size: 1079 650 1390 2579
Fragments arranged by size:
2579 1390 1079 650
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3823 4688 4986 3823
Size: 865 298 4535
Fragments arranged by size:
4535 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4361 882
Size: 617 2862 2219
Fragments arranged by size:
2862 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2044 3489 493
Size: 711 840 1445 2702
Fragments arranged by size:
2702 1445 840 711
BsaBI GATnn'nnATC
Cuts at: 5692 5692
Size: 5698
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3505 3887 4522 275
Size: 53 83 186 936 1972 382 635 1451
Fragments arranged by size:
1972 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4131 4529 4803 4950 5361 4131
Size: 398 274 147 411 4468
Fragments arranged by size:
4468 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2551 2581 1178
Size: 30 165 30 1148 30 4295
Fragments arranged by size:
4295 1148 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2321 2572 1186
Size: 1135 251 4312
Fragments arranged by size:
4312 1135 251
BseYI C'CCAG_C
Cuts at: 2906 2937 4901 5397 2906
Size: 31 1964 496 3207
Fragments arranged by size:
3207 1964 496 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2174 2417 1163
Size: 573 438 243 4444
Fragments arranged by size:
4444 573 438 243
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2569 3148 3909 4937 1540
Size: 275 186 568 579 761 1028 2301
Fragments arranged by size:
2301 1028 761 579 568 275 186
BsiHKAI G_wGCw'C
Cuts at: 2896 3759 3844 4915 2896
Size: 863 85 1071 3679
Fragments arranged by size:
3679 1071 863 85
BsmI GAATG_Cn'
Cuts at: 2471 3209 3302 2471
Size: 738 93 4867
Fragments arranged by size:
4867 738 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2376 2896 3017 3759 3844 4915 1391
Size: 399 586 520 121 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 586 520 399 121 85
BspEI T'CCGG_A
Cuts at: 5361 5361
Size: 5698
BspHI T'CATG_A
Cuts at: 2485 3587 5317 2485
Size: 1102 1730 2866
Fragments arranged by size:
2866 1730 1102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3053 844
Size: 2209 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3585 1394
Size: 429 1762 3507
Fragments arranged by size:
3507 1762 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4193 4367 65
Size: 1183 2945 174 1396
Fragments arranged by size:
2945 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4342 4529 1700
Size: 110 2532 187 2869
Fragments arranged by size:
2869 2532 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5698
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5698
BssSI C'ACGA_G
Cuts at: 2889 3758 4770 2889
Size: 869 1012 3817
Fragments arranged by size:
3817 1012 869
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5698
BstBI TT'CG_AA
Cuts at: 4502 4502
Size: 5698
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5698
BstYI r'GATC_y
Cuts at: 1972 3028 3395 3780 3797 5249 5335 5347 5693
Size: 1056 367 385 17 1452 86 12 346
Cuts at: 5693 1972
Size: 1977
Fragments arranged by size:
1977 1452 1056 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4207
Fragments arranged by size:
4207 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4959
Fragments arranged by size:
4959 683 56
BtsI GCAGTG_nn'
Cuts at: 3214 4008 4028 3214
Size: 794 20 4884
Fragments arranged by size:
4884 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5663
DraI TTT'AAA
Cuts at: 3002 3002
Size: 5698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4705 809
Size: 507 3389 1802
Fragments arranged by size:
3389 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2385 2688 3145
Size: 54 1425 50 275 18 555 303 457
Cuts at: 3145 4034 5663 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 555 457 303 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3145 1537
Size: 275 1333 4090
Fragments arranged by size:
4090 1333 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2796 2925 3628 1353
Size: 1443 129 703 3423
Fragments arranged by size:
3423 1443 703 129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4285 4659 4805 387
Size: 1064 138 2696 374 146 1280
Fragments arranged by size:
2696 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2318 2729 2968 3761 4358 5145 5413
Size: 393 686 411 239 793 597 787 268
Cuts at: 5413 1239
Size: 1524
Fragments arranged by size:
1524 793 787 686 597 411 393 268
239
EcoO109I rG'GnC_Cy
Cuts at: 2117 2117
Size: 5698
EcoRI G'AATT_C
Cuts at: 3007 3385 3007
Size: 378 5320
EcoRV GAT'ATC
Cuts at: 2509 2509
Size: 5698
FspI TGC'GCA
Cuts at: 4204 4204
Size: 5698
HaeII r_GCGC'y
Cuts at: 1678 4845 1678
Size: 3167 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2252 2284 2527 2551 2559
Size: 32 135 32 680 32 243 24 8
Cuts at: 2559 2583 2643 2675 4339 4371 4413 4445 1373
Size: 24 60 32 1664 32 42 32 2626
Fragments arranged by size:
2626 1664 680 243 135 60 42 32
32 32 32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3042 3288 669
Size: 988 281 1104 246 3079
Fragments arranged by size:
3079 1104 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2063 2752 748
Size: 1315 689 3694
Fragments arranged by size:
3694 1315 689
HpaI GTT'AAC
Cuts at: 3288 3288
Size: 5698
KpnI G_GTAC'C
Cuts at: 3023 3023
Size: 5698
MfeI C'AATT_G
Cuts at: 3297 3297
Size: 5698
MluI A'CGCG_T
Cuts at: 5574 5574
Size: 5698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2037 4812 4996 1305
Size: 732 2775 184 2007
Fragments arranged by size:
2775 2007 732 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2690 10
Size: 54 1425 343 858 3018
Fragments arranged by size:
3018 1425 858 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3656 4015 4174 518
Size: 648 2490 359 159 2042
Fragments arranged by size:
2490 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2401 3114 3791 4939 5184 1606
Size: 795 713 677 1148 245 2120
Fragments arranged by size:
2120 1148 795 713 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4207
Fragments arranged by size:
4207 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5698
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5698
NotI GC'GGCC_GC
Cuts at: 3145 3145
Size: 5698
NspI r_CATG'y
Cuts at: 1132 1229 2240 3056 4601 1132
Size: 97 1011 816 1545 2229
Fragments arranged by size:
2229 1545 1011 816 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5698
PciI A'CATG_T
Cuts at: 4597 4597
Size: 5698
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5698
PmeI GTTT'AAAC
Cuts at: 3002 3002
Size: 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 5280 5312 3767
Size: 32 1481 32 4153
Fragments arranged by size:
4153 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2117 2117
Size: 5698
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5698
PsiI TTA'TAA
Cuts at: 3268 3268
Size: 5698
PstI C_TGCA'G
Cuts at: 830 3050 830
Size: 2220 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5698
SalI G'TCGA_C
Cuts at: 1936 3040 1936
Size: 1104 4594
SbfI CC_TGCA'GG
Cuts at: 3050 3050
Size: 5698
ScaI AGT'ACT
Cuts at: 3946 5658 3946
Size: 1712 3986
SfcI C'TryA_G
Cuts at: 826 1045 2848 2977 3046 4181 4862 5053 826
Size: 219 1803 129 69 1135 681 191 1471
Fragments arranged by size:
1803 1471 1135 681 219 191 129 69
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5698
SmaI CCC'GGG
Cuts at: 1872 3025 1872
Size: 1153 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3802 4703 4965 820
Size: 197 926 1859 901 262 1553
Fragments arranged by size:
1859 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3489 493
Size: 2996 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5698
SphI G_CATG'C
Cuts at: 1132 2240 3056 1132
Size: 1108 816 3774
Fragments arranged by size:
3774 1108 816
SspI AAT'ATT
Cuts at: 5 52 3622 5
Size: 47 3570 2081
Fragments arranged by size:
3570 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5698
StyI C'CwwG_G
Cuts at: 513 1065 2004 2365 2520 3137 513
Size: 552 939 361 155 617 3074
Fragments arranged by size:
3074 939 617 552 361 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3725 3742 3895 4080 4525 325
Size: 1255 2145 17 153 185 445 1498
Fragments arranged by size:
2145 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3944 5656 96
Size: 274 80 33 51 1443 1967 1712 138
Fragments arranged by size:
1967 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2564 2596 5658 5690 2564
Size: 32 3062 32 2572
Fragments arranged by size:
3062 2572 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2027 1316
Size: 711 4987
XbaI T'CTAG_A
Cuts at: 3034 3034
Size: 5698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2527 2527
Size: 5698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5698
XmaI C'CCGG_G
Cuts at: 1870 3023 1870
Size: 1153 4545
XmnI GAAnn'nnTTC
Cuts at: 2136 3827 2136
Size: 1691 4007
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3506 276
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI