Restriction map for
pFN21AE0758
(Circular) (Six-base) MAPSORT of: pFN21AE0758.seq Check: 8424 from: 1 to: 6001
pFN21AE0758 6001 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3811 278
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3344 1937
Size: 1407 4594
Acc65I G'GTAC_C
Cuts at: 3322 3322
Size: 6001
AclI AA'CG_TT
Cuts at: 1563 4128 4501 1563
Size: 2565 373 3063
Fragments arranged by size:
3063 2565 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2713 2797 2965 3217 3271 4064 5448 5716
Size: 1081 84 168 252 54 793 1384 268
Cuts at: 5716 1632
Size: 1917
Fragments arranged by size:
1917 1384 1081 793 268 252 168 84
54
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5804
AflIII A'CryG_T
Cuts at: 1203 2755 4900 5877 1203
Size: 1552 2145 977 1327
Fragments arranged by size:
2145 1552 1327 977
AgeI A'CCGG_T
Cuts at: 4832 4832
Size: 6001
AhdI GACnn_n'nnGTC
Cuts at: 4730 4730
Size: 6001
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6001
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6001
AlwNI CAG_nnn'CTG
Cuts at: 3744 5316 3744
Size: 1572 4429
ApaLI G'TGCA_C
Cuts at: 4058 5214 4058
Size: 1156 4845
ApoI r'AATT_y
Cuts at: 1493 2595 2679 2763 3183 3310 3536 3688 5797
Size: 1102 84 84 420 127 226 152 2109
Cuts at: 5797 1493
Size: 1697
Fragments arranged by size:
2109 1697 1102 420 226 152 127 84
84
AseI AT'TA_AT
Cuts at: 160 1032 2394 4555 160
Size: 872 1362 2161 1606
Fragments arranged by size:
2161 1606 1362 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
AvaI C'yCGr_G
Cuts at: 1870 1943 3326 1870
Size: 73 1383 4545
Fragments arranged by size:
4545 1383 73
BamHI G'GATC_C
Cuts at: 3331 3698 3331
Size: 367 5634
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3322 4777 618
Size: 325 217 627 1535 1455 1842
Fragments arranged by size:
1842 1535 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3320 1391
Size: 1929 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2423 928
Size: 971 524 4506
Fragments arranged by size:
4506 971 524
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4192 4226 1903
Size: 34 50 34 2171 34 3678
Fragments arranged by size:
3678 2171 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3893 5103 3893
Size: 1210 4791
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6001
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4612 136
Size: 107 122 71 631 605 2940 1525
Fragments arranged by size:
2940 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5996 5996
Size: 6001
BlpI GC'TnA_GC
Cuts at: 3418 3418
Size: 6001
Bme1580I G_kGCm'C
Cuts at: 1790 4062 5218 1790
Size: 2272 1156 2573
Fragments arranged by size:
2573 2272 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6001
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2065 3732 4690
Size: 416 53 83 399 24 648 1667 958
Cuts at: 4690 442
Size: 1753
Fragments arranged by size:
1753 1667 958 648 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6001
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2030 3045 3129 3213 4661 1239
Size: 791 1015 84 84 1448 2579
Fragments arranged by size:
2579 1448 1015 791 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4126 4991 5289 4126
Size: 865 298 4838
Fragments arranged by size:
4838 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2035 4664 882
Size: 617 536 2629 2219
Fragments arranged by size:
2629 2219 617 536
BsaAI yAC'GTr
Cuts at: 493 1204 3792 493
Size: 711 2588 2702
Fragments arranged by size:
2702 2588 711
BsaBI GATnn'nnATC
Cuts at: 5995 5995
Size: 6001
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3808 4190 4825 275
Size: 53 83 186 936 2275 382 635 1451
Fragments arranged by size:
2275 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4434 4832 5106 5253 5664 4434
Size: 398 274 147 411 4771
Fragments arranged by size:
4771 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5776
Fragments arranged by size:
5776 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6001
BseYI C'CCAG_C
Cuts at: 5204 5700 5204
Size: 496 5505
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5428
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3451 4212 5240 1540
Size: 275 186 1450 761 1028 2301
Fragments arranged by size:
2301 1450 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4062 4147 5218 4062
Size: 85 1071 4845
Fragments arranged by size:
4845 1071 85
BsmI GAATG_Cn'
Cuts at: 3131 3512 3605 3131
Size: 381 93 5527
Fragments arranged by size:
5527 381 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3320 4062 4147 5218 1391
Size: 399 1530 742 85 1071 2174
Fragments arranged by size:
2174 1530 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5664 5664
Size: 6001
BspHI T'CATG_A
Cuts at: 3890 5620 3890
Size: 1730 4271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3356 844
Size: 2512 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3888 1394
Size: 429 2065 3507
Fragments arranged by size:
3507 2065 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3298 4496 4670 65
Size: 1183 2050 1198 174 1396
Fragments arranged by size:
2050 1396 1198 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4645 4832 1700
Size: 110 2835 187 2869
Fragments arranged by size:
2869 2835 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6001
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6001
BssSI C'ACGA_G
Cuts at: 4061 5073 4061
Size: 1012 4989
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6001
BstBI TT'CG_AA
Cuts at: 4805 4805
Size: 6001
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6001
BstXI CCAn_nnnn'nTGG
Cuts at: 2825 2825
Size: 6001
BstYI r'GATC_y
Cuts at: 1972 3331 3698 4083 4100 5552 5638 5650 5996
Size: 1359 367 385 17 1452 86 12 346
Cuts at: 5996 1972
Size: 1977
Fragments arranged by size:
1977 1452 1359 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2915 2915
Size: 6001
BtgI C'CryG_G
Cuts at: 513 1065 2093 513
Size: 552 1028 4421
Fragments arranged by size:
4421 1028 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2727 487
Size: 683 56 1501 3761
Fragments arranged by size:
3761 1501 683 56
BtsI GCAGTG_nn'
Cuts at: 3259 3517 4311 4331 3259
Size: 258 794 20 4929
Fragments arranged by size:
4929 794 258 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6001
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2866 2901 529
Size: 35 2302 35 3629
Fragments arranged by size:
3629 2302 35 35
DraI TTT'AAA
Cuts at: 3305 3305
Size: 6001
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2427 5008 809
Size: 507 1111 2581 1802
Fragments arranged by size:
2581 1802 1111 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3448 4337 5966
Size: 54 1425 50 275 18 1618 889 1629
Cuts at: 5966 8
Size: 43
Fragments arranged by size:
1629 1618 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3448 1537
Size: 275 1636 4090
Fragments arranged by size:
4090 1636 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2135 3931 1353
Size: 782 1796 3423
Fragments arranged by size:
3423 1796 782
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4588 4962 5108 387
Size: 1064 138 2999 374 146 1280
Fragments arranged by size:
2999 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2713 2797 2965 3045 3129 3213
Size: 393 398 683 84 168 80 84 84
Cuts at: 3213 3217 3271 4064 4661 5448 5716 1239
Size: 4 54 793 597 787 268 1524
Fragments arranged by size:
1524 793 787 683 597 398 393 268
168 84 84 84 80 54 4
EcoRI G'AATT_C
Cuts at: 2595 2679 2763 3183 3310 3688 2595
Size: 84 84 420 127 378 4908
Fragments arranged by size:
4908 420 378 127 84 84
FspI TGC'GCA
Cuts at: 4507 4507
Size: 6001
HaeII r_GCGC'y
Cuts at: 1678 5148 1678
Size: 3470 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3064 3096 4642 4674 4716
Size: 32 135 32 1492 32 1546 32 42
Cuts at: 4716 4748 1373
Size: 32 2626
Fragments arranged by size:
2626 1546 1492 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3345 3591 669
Size: 988 281 1407 246 3079
Fragments arranged by size:
3079 1407 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6001
HpaI GTT'AAC
Cuts at: 3591 3591
Size: 6001
KpnI G_GTAC'C
Cuts at: 3326 3326
Size: 6001
MfeI C'AATT_G
Cuts at: 3600 3600
Size: 6001
MluI A'CGCG_T
Cuts at: 5877 5877
Size: 6001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2055 5115 5299 1305
Size: 750 3060 184 2007
Fragments arranged by size:
3060 2007 750 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4179
Fragments arranged by size:
4179 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2688 2940 3040 3959 4318 4477 518
Size: 648 1522 252 100 919 359 159 2042
Fragments arranged by size:
2042 1522 919 648 359 252 159 100
MspA1I CmG'CkG
Cuts at: 1606 3237 3417 4094 5242 5487 1606
Size: 1631 180 677 1148 245 2120
Fragments arranged by size:
2120 1631 1148 677 245 180
NcoI C'CATG_G
Cuts at: 513 1065 2093 513
Size: 552 1028 4421
Fragments arranged by size:
4421 1028 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6001
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6001
NotI GC'GGCC_GC
Cuts at: 3448 3448
Size: 6001
NspI r_CATG'y
Cuts at: 1132 1229 2245 2759 2877 3359 4904 1132
Size: 97 1016 514 118 482 1545 2229
Fragments arranged by size:
2229 1545 1016 514 482 118 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6001
PciI A'CATG_T
Cuts at: 2755 4900 2755
Size: 2145 3856
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2308 1108
Size: 1200 4801
PmeI GTTT'AAAC
Cuts at: 3305 3305
Size: 6001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2015 2047 4070 4102 5583 5615 2015
Size: 32 2023 32 1481 32 2401
Fragments arranged by size:
2401 2023 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6001
PsiI TTA'TAA
Cuts at: 3571 3571
Size: 6001
PstI C_TGCA'G
Cuts at: 830 3353 830
Size: 2523 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6001
PvuII CAG'CTG
Cuts at: 3237 3237
Size: 6001
SalI G'TCGA_C
Cuts at: 1936 3343 1936
Size: 1407 4594
SapI GCTCTTCn'nnn_
Cuts at: 2135 2135
Size: 6001
SbfI CC_TGCA'GG
Cuts at: 3353 3353
Size: 6001
ScaI AGT'ACT
Cuts at: 4249 5961 4249
Size: 1712 4289
SexAI A'CCwGG_T
Cuts at: 2025 2025
Size: 6001
SfcI C'TryA_G
Cuts at: 826 1045 3238 3349 4484 5165 5356 826
Size: 219 2193 111 1135 681 191 1471
Fragments arranged by size:
2193 1471 1135 681 219 191 111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6001
SmaI CCC'GGG
Cuts at: 1872 3328 1872
Size: 1456 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4105 5006 5268 820
Size: 197 926 2162 901 262 1553
Fragments arranged by size:
2162 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3792 493
Size: 3299 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6001
SphI G_CATG'C
Cuts at: 1132 3359 1132
Size: 2227 3774
SspI AAT'ATT
Cuts at: 5 52 2545 3925 5
Size: 47 2493 1380 2081
Fragments arranged by size:
2493 2081 1380 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6001
StyI C'CwwG_G
Cuts at: 513 1065 2093 2253 3440 513
Size: 552 1028 160 1187 3074
Fragments arranged by size:
3074 1187 1028 552 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4028 4045 4198 4383 4828 325
Size: 1255 2448 17 153 185 445 1498
Fragments arranged by size:
2448 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2990 4247 5959
Size: 274 80 33 51 1443 1013 1257 1712
Cuts at: 5959 96
Size: 138
Fragments arranged by size:
1712 1443 1257 1013 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5961 5993 5961
Size: 32 5969
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6001
XbaI T'CTAG_A
Cuts at: 3337 3337
Size: 6001
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6001
XmaI C'CCGG_G
Cuts at: 1870 3326 1870
Size: 1456 4545
XmnI GAAnn'nnTTC
Cuts at: 2345 2615 2726 2810 4130 2345
Size: 270 111 84 1320 4216
Fragments arranged by size:
4216 1320 270 111 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3809 276
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SfiI SfoI SrfI SwaI XcmI