Restriction map for
pFN21AE0757
(Circular) (Six-base) MAPSORT of: pFN21AE0757.seq Check: 9081 from: 1 to: 5695
pFN21AE0757 5695 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3505 278
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2556 3038 1937
Size: 619 482 4594
Fragments arranged by size:
4594 619 482
Acc65I G'GTAC_C
Cuts at: 3016 3016
Size: 5695
AclI AA'CG_TT
Cuts at: 1563 3822 4195 1563
Size: 2259 373 3063
Fragments arranged by size:
3063 2259 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2056 3758 5142 5410 1632
Size: 424 1702 1384 268 1917
Fragments arranged by size:
1917 1702 1384 424 268
AfeI AGC'GCT
Cuts at: 2512 2512
Size: 5695
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5498
AflIII A'CryG_T
Cuts at: 1203 4594 5571 1203
Size: 3391 977 1327
Fragments arranged by size:
3391 1327 977
AgeI A'CCGG_T
Cuts at: 4526 4526
Size: 5695
AhdI GACnn_n'nnGTC
Cuts at: 4424 4424
Size: 5695
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5695
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5695
AlwNI CAG_nnn'CTG
Cuts at: 2265 2758 2853 3438 5010 2265
Size: 493 95 585 1572 2950
Fragments arranged by size:
2950 1572 585 493 95
ApaLI G'TGCA_C
Cuts at: 2767 3752 4908 2767
Size: 985 1156 3554
Fragments arranged by size:
3554 1156 985
ApoI r'AATT_y
Cuts at: 1493 3004 3230 3382 5491 1493
Size: 1511 226 152 2109 1697
Fragments arranged by size:
2109 1697 1511 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4249 160
Size: 872 3217 1606
Fragments arranged by size:
3217 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
AvaI C'yCGr_G
Cuts at: 1870 1943 2684 2762 2831 2942 3020 1870
Size: 73 741 78 69 111 78 4545
Fragments arranged by size:
4545 741 111 78 78 73 69
BamHI G'GATC_C
Cuts at: 2307 3025 3392 2307
Size: 718 367 4610
Fragments arranged by size:
4610 718 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2366 2795 3016 4471 618
Size: 325 217 627 579 429 221 1455 1842
Fragments arranged by size:
1842 1455 627 579 429 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2181 2567 2604 2619 3014 1391
Size: 790 386 37 15 395 4072
Fragments arranged by size:
4072 790 395 386 37 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4724
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3886 3920 1903
Size: 34 50 34 1865 34 3678
Fragments arranged by size:
3678 1865 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3587 4797 3587
Size: 1210 4485
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5695
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2058 4306 136
Size: 107 122 71 631 605 386 2248 1525
Fragments arranged by size:
2248 1525 631 605 386 122 107 71
BglII A'GATC_T
Cuts at: 5690 5690
Size: 5695
BlpI GC'TnA_GC
Cuts at: 2741 3112 2741
Size: 371 5324
Bme1580I G_kGCm'C
Cuts at: 1790 2138 2278 2771 2798 3756 4912 1790
Size: 348 140 493 27 958 1156 2573
Fragments arranged by size:
2573 1156 958 493 348 140 27
BmgBI CAC'GTC
Cuts at: 1318 2956 1318
Size: 1638 4057
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2265 2385 3426
Size: 416 53 83 399 24 848 120 1041
Cuts at: 3426 4384 442
Size: 958 1753
Fragments arranged by size:
1753 1041 958 848 416 399 120 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 4355 1239
Size: 974 2142 2579
Fragments arranged by size:
2579 2142 974
Bpu10I CC'TnA_GC
Cuts at: 2295 2295
Size: 5695
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2693 2852 3820 4685 4983 2693
Size: 159 968 865 298 3405
Fragments arranged by size:
3405 968 865 298 159
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2136 4358 882
Size: 617 637 2222 2219
Fragments arranged by size:
2222 2219 637 617
BsaAI yAC'GTr
Cuts at: 493 1204 3486 493
Size: 711 2282 2702
Fragments arranged by size:
2702 2282 711
BsaBI GATnn'nnATC
Cuts at: 2312 5689 2312
Size: 3377 2318
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3502 3884 4519 275
Size: 53 83 186 936 1969 382 635 1451
Fragments arranged by size:
1969 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4128 4526 4800 4947 5358 4128
Size: 398 274 147 411 4465
Fragments arranged by size:
4465 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2549 2579 1178
Size: 30 165 30 1146 30 4294
Fragments arranged by size:
4294 1146 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5695
BseYI C'CCAG_C
Cuts at: 2507 2518 2728 4898 5394 2507
Size: 11 210 2170 496 2808
Fragments arranged by size:
2808 2170 496 210 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2866 1163
Size: 573 1130 3992
Fragments arranged by size:
3992 1130 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2065 3145 3906 4934 1540
Size: 275 186 64 1080 761 1028 2301
Fragments arranged by size:
2301 1080 1028 761 275 186 64
BsiHKAI G_wGCw'C
Cuts at: 2174 2181 2486 2771 3756 3841 4912 2174
Size: 7 305 285 985 85 1071 2957
Fragments arranged by size:
2957 1071 985 305 285 85 7
BsmI GAATG_Cn'
Cuts at: 3206 3299 3206
Size: 93 5602
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2138 2174 2181 2278 2486 2567 2604
Size: 399 348 36 7 97 208 81 37
Cuts at: 2604 2619 2771 2798 3014 3756 3841 4912 1391
Size: 15 152 27 216 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 348 216 208 152
97 85 81 37 36 27 15 7
BspEI T'CCGG_A
Cuts at: 5358 5358
Size: 5695
BspHI T'CATG_A
Cuts at: 2181 3584 5314 2181
Size: 1403 1730 2562
Fragments arranged by size:
2562 1730 1403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3050 844
Size: 2206 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2745 2899 3582 1394
Size: 429 922 154 683 3507
Fragments arranged by size:
3507 922 683 429 154
BsrDI GCAATG_nn'
Cuts at: 65 1248 4190 4364 65
Size: 1183 2942 174 1396
Fragments arranged by size:
2942 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2663 4339 4526 1700
Size: 110 853 1676 187 2869
Fragments arranged by size:
2869 1676 853 187 110
BsrGI T'GTAC_A
Cuts at: 96 2574 96
Size: 2478 3217
BssHII G'CGCG_C
Cuts at: 1925 2076 2927 1925
Size: 151 851 4693
Fragments arranged by size:
4693 851 151
BssSI C'ACGA_G
Cuts at: 3755 4767 3755
Size: 1012 4683
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2126 2653 2758 2775 2853 794
Size: 1332 527 105 17 78 3636
Fragments arranged by size:
3636 1332 527 105 78 17
BstBI TT'CG_AA
Cuts at: 4499 4499
Size: 5695
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5695
BstXI CCAn_nnnn'nTGG
Cuts at: 2728 2728
Size: 5695
BstYI r'GATC_y
Cuts at: 1972 2307 2325 2405 3025 3392 3777 3794 5246
Size: 335 18 80 620 367 385 17 1452
Cuts at: 5246 5332 5344 5690 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 620 385 367 346 335 86
80 18 17 12
Bsu36I CC'TnA_GG
Cuts at: 2253 2362 2253
Size: 109 5586
BtgI C'CryG_G
Cuts at: 513 1065 2534 2604 2610 2715 2782 513
Size: 552 1469 70 6 105 67 3426
Fragments arranged by size:
3426 1469 552 105 70 67 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4956
Fragments arranged by size:
4956 683 56
BtsI GCAGTG_nn'
Cuts at: 2854 3211 4005 4025 2854
Size: 357 794 20 4524
Fragments arranged by size:
4524 794 357 20
ClaI AT'CG_AT
Cuts at: 1554 2316 1554
Size: 762 4933
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2857 2892 529
Size: 35 2293 35 3332
Fragments arranged by size:
3332 2293 35 35
DraI TTT'AAA
Cuts at: 2999 2999
Size: 5695
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2552 4702 809
Size: 507 1236 2150 1802
Fragments arranged by size:
2150 1802 1236 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2062 2607 2718
Size: 54 1425 50 275 18 232 545 111
Cuts at: 2718 3142 4031 5660 8
Size: 424 889 1629 43
Fragments arranged by size:
1629 1425 889 545 424 275 232 111
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2062 3142 1537
Size: 275 250 1080 4090
Fragments arranged by size:
4090 1080 275 250
EarI CTCTTCn'nnn_
Cuts at: 1353 3625 1353
Size: 2272 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2039 4282 4656 4802 387
Size: 1064 138 450 2243 374 146 1280
Fragments arranged by size:
2243 1280 1064 450 374 146 138
EcoICRI GAG'CTC
Cuts at: 2179 2179
Size: 5695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2056 2213 3758 4355 5142 5410 1239
Size: 393 424 157 1545 597 787 268 1524
Fragments arranged by size:
1545 1524 787 597 424 393 268 157
EcoNI CCTnn'n_nnAGG
Cuts at: 2150 2150
Size: 5695
EcoRI G'AATT_C
Cuts at: 3004 3382 3004
Size: 378 5317
FspI TGC'GCA
Cuts at: 4201 4201
Size: 5695
HaeII r_GCGC'y
Cuts at: 1678 2514 2736 4842 1678
Size: 836 222 2106 2531
Fragments arranged by size:
2531 2106 836 222
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2133 2165 2549 2581 4336
Size: 32 135 32 561 32 384 32 1755
Cuts at: 4336 4368 4410 4442 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1755 561 384 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2557 3039 3285 669
Size: 988 281 619 482 246 3079
Fragments arranged by size:
3079 988 619 482 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5695
HpaI GTT'AAC
Cuts at: 3285 3285
Size: 5695
KpnI G_GTAC'C
Cuts at: 3020 3020
Size: 5695
MfeI C'AATT_G
Cuts at: 3294 3294
Size: 5695
MluI A'CGCG_T
Cuts at: 5571 5571
Size: 5695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4809 4993 1305
Size: 3504 184 2007
Fragments arranged by size:
3504 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3873
Fragments arranged by size:
3873 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3653 4012 4171 518
Size: 648 2487 359 159 2042
Fragments arranged by size:
2487 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2536 2612 2740 3111 3788 4936 5181 1606
Size: 930 76 128 371 677 1148 245 2120
Fragments arranged by size:
2120 1148 930 677 371 245 128 76
NaeI GCC'GGC
Cuts at: 2665 2665
Size: 5695
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5143
NdeI CA'TA_TG
Cuts at: 387 2413 387
Size: 2026 3669
NgoMIV G'CCGG_C
Cuts at: 2663 2663
Size: 5695
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5695
NotI GC'GGCC_GC
Cuts at: 3142 3142
Size: 5695
NspI r_CATG'y
Cuts at: 1132 1229 2495 2656 3053 4598 1132
Size: 97 1266 161 397 1545 2229
Fragments arranged by size:
2229 1545 1266 397 161 97
PasI CC'CwG_GG
Cuts at: 1348 2040 1348
Size: 692 5003
PciI A'CATG_T
Cuts at: 4594 4594
Size: 5695
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2387 1108
Size: 1279 4416
PfoI T'CCnGG_A
Cuts at: 2328 2328
Size: 5695
PmeI GTTT'AAAC
Cuts at: 2999 2999
Size: 5695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 5277 5309 3764
Size: 32 1481 32 4150
Fragments arranged by size:
4150 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5695
PsiI TTA'TAA
Cuts at: 3265 3265
Size: 5695
PstI C_TGCA'G
Cuts at: 830 2552 2754 3047 830
Size: 1722 202 293 3478
Fragments arranged by size:
3478 1722 293 202
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5695
SacI G_AGCT'C
Cuts at: 2181 2181
Size: 5695
SacII CC_GC'GG
Cuts at: 2537 2613 2537
Size: 76 5619
SalI G'TCGA_C
Cuts at: 1936 2555 3037 1936
Size: 619 482 4594
Fragments arranged by size:
4594 619 482
SbfI CC_TGCA'GG
Cuts at: 2754 3047 2754
Size: 293 5402
ScaI AGT'ACT
Cuts at: 3943 5655 3943
Size: 1712 3983
SfcI C'TryA_G
Cuts at: 826 1045 2548 2750 3043 4178 4859 5050 826
Size: 219 1503 202 293 1135 681 191 1471
Fragments arranged by size:
1503 1471 1135 681 293 219 202 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5695
SmaI CCC'GGG
Cuts at: 1872 2686 3022 1872
Size: 814 336 4545
Fragments arranged by size:
4545 814 336
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2708 2867 3799 4700 4962 820
Size: 197 926 765 159 932 901 262 1553
Fragments arranged by size:
1553 932 926 901 765 262 197 159
SnaBI TAC'GTA
Cuts at: 493 3486 493
Size: 2993 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5695
SphI G_CATG'C
Cuts at: 1132 2656 3053 1132
Size: 1524 397 3774
Fragments arranged by size:
3774 1524 397
SrfI GCCC'GGGC
Cuts at: 2686 2686
Size: 5695
SspI AAT'ATT
Cuts at: 5 52 3619 5
Size: 47 3567 2081
Fragments arranged by size:
3567 2081 47
StuI AGG'CCT
Cuts at: 1517 2251 2529 1517
Size: 734 278 4683
Fragments arranged by size:
4683 734 278
StyI C'CwwG_G
Cuts at: 513 1065 2922 2979 3134 513
Size: 552 1857 57 155 3074
Fragments arranged by size:
3074 1857 552 155 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3722 3739 3892 4077 4522 325
Size: 1255 2142 17 153 185 445 1498
Fragments arranged by size:
2142 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2574 3941 5653
Size: 274 80 33 51 1443 597 1367 1712
Cuts at: 5653 96
Size: 138
Fragments arranged by size:
1712 1443 1367 597 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2292 2324 5655 5687 2292
Size: 32 3331 32 2300
Fragments arranged by size:
3331 2300 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5695
XbaI T'CTAG_A
Cuts at: 3031 3031
Size: 5695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2159 2980 2159
Size: 821 4874
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5695
XmaI C'CCGG_G
Cuts at: 1870 2684 3020 1870
Size: 814 336 4545
Fragments arranged by size:
4545 814 336
XmnI GAAnn'nnTTC
Cuts at: 3824 3824
Size: 5695
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3503 276
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SacI SacII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoO109I EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI
SapI SexAI SfiI SfoI SwaI