Restriction map for
pFN21AE0755
(Circular) (Six-base) MAPSORT of: pFN21AE0755.seq Check: 6619 from: 1 to: 5692
pFN21AE0755 5692 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3502 278
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3035 1937
Size: 1098 4594
Acc65I G'GTAC_C
Cuts at: 3013 3013
Size: 5692
AclI AA'CG_TT
Cuts at: 1563 3819 4192 1563
Size: 2256 373 3063
Fragments arranged by size:
3063 2256 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2552 2864 3755 5139 5407 1632
Size: 920 312 891 1384 268 1917
Fragments arranged by size:
1917 1384 920 891 312 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5495
AflIII A'CryG_T
Cuts at: 1203 4591 5568 1203
Size: 3388 977 1327
Fragments arranged by size:
3388 1327 977
AgeI A'CCGG_T
Cuts at: 2279 4523 2279
Size: 2244 3448
AhdI GACnn_n'nnGTC
Cuts at: 2236 4421 2236
Size: 2185 3507
AleI CACnn'nnGTG
Cuts at: 1166 2778 1166
Size: 1612 4080
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5692
AlwNI CAG_nnn'CTG
Cuts at: 2192 2765 3435 5007 2192
Size: 573 670 1572 2877
Fragments arranged by size:
2877 1572 670 573
ApaI G_GGCC'C
Cuts at: 2354 2561 2354
Size: 207 5485
ApaLI G'TGCA_C
Cuts at: 3749 4905 3749
Size: 1156 4536
ApoI r'AATT_y
Cuts at: 1493 3001 3227 3379 5488 1493
Size: 1508 226 152 2109 1697
Fragments arranged by size:
2109 1697 1508 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4246 160
Size: 872 3214 1606
Fragments arranged by size:
3214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
AvaI C'yCGr_G
Cuts at: 1870 1943 2271 2374 3017 1870
Size: 73 328 103 643 4545
Fragments arranged by size:
4545 643 328 103 73
AvrII C'CTAG_G
Cuts at: 2891 2891
Size: 5692
BamHI G'GATC_C
Cuts at: 2399 3022 3389 2399
Size: 623 367 4702
Fragments arranged by size:
4702 623 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2282 2591 2883 3013 4468
Size: 325 217 627 495 309 292 130 1455
Cuts at: 4468 618
Size: 1842
Fragments arranged by size:
1842 1455 627 495 325 309 292 217
130
BanII G_rGCy'C
Cuts at: 1391 2095 2354 2561 2933 3011 1391
Size: 704 259 207 372 78 4072
Fragments arranged by size:
4072 704 372 259 207 78
BbeI G_GCGC'C
Cuts at: 2887 2887
Size: 5692
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2852 928
Size: 971 953 3768
Fragments arranged by size:
3768 971 953
BbvCI CC'TCA_GC
Cuts at: 2576 2965 2576
Size: 389 5303
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3883 3917 1903
Size: 34 1946 34 3678
Fragments arranged by size:
3678 1946 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3584 4794 3584
Size: 1210 4482
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5692
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2118 2197 2928
Size: 107 122 71 631 605 446 79 731
Cuts at: 2928 4303 136
Size: 1375 1525
Fragments arranged by size:
1525 1375 731 631 605 446 122 107
79 71
BglII A'GATC_T
Cuts at: 5687 5687
Size: 5692
BlpI GC'TnA_GC
Cuts at: 2328 3109 2328
Size: 781 4911
Bme1580I G_kGCm'C
Cuts at: 1790 2354 2561 2594 3753 4909 1790
Size: 564 207 33 1159 1156 2573
Fragments arranged by size:
2573 1159 1156 564 207 33
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5692
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2407 3423 4381
Size: 416 53 83 399 24 990 1016 958
Cuts at: 4381 442
Size: 1753
Fragments arranged by size:
1753 1016 990 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5692
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2410 2467 2410
Size: 57 5635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 2153 2909 4352 1239
Size: 797 117 756 1443 2579
Fragments arranged by size:
2579 1443 797 756 117
Bpu10I CC'TnA_GC
Cuts at: 2576 2965 2576
Size: 389 5303
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3817 4682 4980 3817
Size: 865 298 4529
Fragments arranged by size:
4529 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2012 4355 882
Size: 617 513 2343 2219
Fragments arranged by size:
2343 2219 617 513
BsaAI yAC'GTr
Cuts at: 493 1204 3483 493
Size: 711 2279 2702
Fragments arranged by size:
2702 2279 711
BsaBI GATnn'nnATC
Cuts at: 5686 5686
Size: 5692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2565 2670 2884 3499
Size: 53 83 186 936 1032 105 214 615
Cuts at: 3499 3881 4516 275
Size: 382 635 1451
Fragments arranged by size:
1451 1032 936 635 615 382 214 186
105 83 53
BsaWI w'CCGG_w
Cuts at: 2279 4125 4523 4797 4944 5355 2279
Size: 1846 398 274 147 411 2616
Fragments arranged by size:
2616 1846 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2942 2972 1178
Size: 30 165 30 1539 30 3898
Fragments arranged by size:
3898 1539 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2383 2452 2669 1186
Size: 1197 69 217 4209
Fragments arranged by size:
4209 1197 217 69
BseYI C'CCAG_C
Cuts at: 2139 2554 2765 2941 4895 5391 2139
Size: 415 211 176 1954 496 2440
Fragments arranged by size:
2440 1954 496 415 211 176
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2519 2955 1163
Size: 573 783 436 3900
Fragments arranged by size:
3900 783 573 436
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3142 3903 4931 1540
Size: 275 186 1141 761 1028 2301
Fragments arranged by size:
2301 1141 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3753 3838 4909 3753
Size: 85 1071 4536
Fragments arranged by size:
4536 1071 85
BsmI GAATG_Cn'
Cuts at: 3203 3296 3203
Size: 93 5599
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2095 2354 2561 2594 2933 3011 3753
Size: 399 305 259 207 33 339 78 742
Cuts at: 3753 3838 4909 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 339 305 259 207
85 78 33
BspEI T'CCGG_A
Cuts at: 5355 5355
Size: 5692
BspHI T'CATG_A
Cuts at: 3581 5311 3581
Size: 1730 3962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3047 844
Size: 2203 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3579 1394
Size: 429 1756 3507
Fragments arranged by size:
3507 1756 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4187 4361 65
Size: 1183 2939 174 1396
Fragments arranged by size:
2939 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2196 2248 2279 2539 4336 4523 1700
Size: 110 386 52 31 260 1797 187 2869
Fragments arranged by size:
2869 1797 386 260 187 110 52 31
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5692
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5692
BssSI C'ACGA_G
Cuts at: 3752 4764 3752
Size: 1012 4680
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2073 794
Size: 1279 4413
BstBI TT'CG_AA
Cuts at: 4496 4496
Size: 5692
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5692
BstYI r'GATC_y
Cuts at: 1972 2399 3022 3389 3774 3791 5243 5329 5341
Size: 427 623 367 385 17 1452 86 12
Cuts at: 5341 5687 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 623 427 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2395 2395
Size: 5692
BtgI C'CryG_G
Cuts at: 513 1065 2004 2439 2490 513
Size: 552 939 435 51 3715
Fragments arranged by size:
3715 939 552 435 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4953
Fragments arranged by size:
4953 683 56
BtsI GCAGTG_nn'
Cuts at: 3208 4002 4022 3208
Size: 794 20 4878
Fragments arranged by size:
4878 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5657
DraI TTT'AAA
Cuts at: 2996 2996
Size: 5692
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4699 809
Size: 507 3383 1802
Fragments arranged by size:
3383 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2194 3139 4028
Size: 54 1425 50 275 18 364 945 889
Cuts at: 4028 5657 8
Size: 1629 43
Fragments arranged by size:
1629 1425 945 889 364 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3139 1537
Size: 275 1327 4090
Fragments arranged by size:
4090 1327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3622 1353
Size: 2269 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2095 2210 4279 4653 4799 387
Size: 1064 138 506 115 2069 374 146 1280
Fragments arranged by size:
2069 1280 1064 506 374 146 138 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2153 2552 2864 2909 3755 4352
Size: 393 404 117 399 312 45 846 597
Cuts at: 4352 5139 5407 1239
Size: 787 268 1524
Fragments arranged by size:
1524 846 787 597 404 399 393 312
268 117 45
EcoO109I rG'GnC_Cy
Cuts at: 2189 2351 2428 2599 2189
Size: 162 77 171 5282
Fragments arranged by size:
5282 171 162 77
EcoRI G'AATT_C
Cuts at: 3001 3379 3001
Size: 378 5314
FseI GG_CCGG'CC
Cuts at: 2543 2543
Size: 5692
FspI TGC'GCA
Cuts at: 2630 4198 2630
Size: 1568 4124
HaeII r_GCGC'y
Cuts at: 1678 2766 2887 4839 1678
Size: 1088 121 1952 2531
Fragments arranged by size:
2531 1952 1088 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2202 2222 2234 2254 2399
Size: 32 135 32 630 20 12 20 145
Cuts at: 2399 2431 2696 2728 4333 4365 4407 4439 1373
Size: 32 265 32 1605 32 42 32 2626
Fragments arranged by size:
2626 1605 630 265 145 135 42 32
32 32 32 32 32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3036 3282 669
Size: 988 281 1098 246 3079
Fragments arranged by size:
3079 1098 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5692
HpaI GTT'AAC
Cuts at: 3282 3282
Size: 5692
KasI G'GCGC_C
Cuts at: 2883 2883
Size: 5692
KpnI G_GTAC'C
Cuts at: 3017 3017
Size: 5692
MfeI C'AATT_G
Cuts at: 3291 3291
Size: 5692
MluI A'CGCG_T
Cuts at: 5568 5568
Size: 5692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2667 4806 4990 1305
Size: 1362 2139 184 2007
Fragments arranged by size:
2139 2007 1362 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3870
Fragments arranged by size:
3870 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2778 3650 4009 4168 518
Size: 648 1612 872 359 159 2042
Fragments arranged by size:
2042 1612 872 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2171 2365 2523 2530 2575 2610 2969 3108
Size: 565 194 158 7 45 35 359 139
Cuts at: 3108 3785 4933 5178 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 565 359 245 194 158
139 45 35 7
NaeI GCC'GGC
Cuts at: 2198 2250 2541 2198
Size: 52 291 5349
Fragments arranged by size:
5349 291 52
NarI GG'CG_CC
Cuts at: 2884 2884
Size: 5692
NcoI C'CATG_G
Cuts at: 513 1065 2004 2439 513
Size: 552 939 435 3766
Fragments arranged by size:
3766 939 552 435
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5692
NgoMIV G'CCGG_C
Cuts at: 2196 2248 2539 2196
Size: 52 291 5349
Fragments arranged by size:
5349 291 52
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5692
NotI GC'GGCC_GC
Cuts at: 3139 3139
Size: 5692
NspI r_CATG'y
Cuts at: 1132 1229 2057 3050 4595 1132
Size: 97 828 993 1545 2229
Fragments arranged by size:
2229 1545 993 828 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5692
PciI A'CATG_T
Cuts at: 4591 4591
Size: 5692
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2320 2765 1108
Size: 1212 445 4035
Fragments arranged by size:
4035 1212 445
PfoI T'CCnGG_A
Cuts at: 2148 2808 2148
Size: 660 5032
PmeI GTTT'AAAC
Cuts at: 2996 2996
Size: 5692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 5274 5306 3761
Size: 32 1481 32 4147
Fragments arranged by size:
4147 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5692
PsiI TTA'TAA
Cuts at: 3262 3262
Size: 5692
PspOMI G'GGCC_C
Cuts at: 2350 2557 2350
Size: 207 5485
PstI C_TGCA'G
Cuts at: 830 3044 830
Size: 2214 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5692
PvuII CAG'CTG
Cuts at: 2365 2530 2575 2610 2969 2365
Size: 165 45 35 359 5088
Fragments arranged by size:
5088 359 165 45 35
SalI G'TCGA_C
Cuts at: 1936 3034 1936
Size: 1098 4594
SbfI CC_TGCA'GG
Cuts at: 3044 3044
Size: 5692
ScaI AGT'ACT
Cuts at: 3940 5652 3940
Size: 1712 3980
SfcI C'TryA_G
Cuts at: 826 1045 2605 3040 4175 4856 5047 826
Size: 219 1560 435 1135 681 191 1471
Fragments arranged by size:
1560 1471 1135 681 435 219 191
SfoI GGC'GCC
Cuts at: 2885 2885
Size: 5692
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
SgrAI Cr'CCGG_yG
Cuts at: 1700 2279 1700
Size: 579 5113
SmaI CCC'GGG
Cuts at: 1872 2273 2376 3019 1872
Size: 401 103 643 4545
Fragments arranged by size:
4545 643 401 103
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3796 4697 4959 820
Size: 197 926 1853 901 262 1553
Fragments arranged by size:
1853 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3483 493
Size: 2990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5692
SphI G_CATG'C
Cuts at: 1132 3050 1132
Size: 1918 3774
SspI AAT'ATT
Cuts at: 5 52 3616 5
Size: 47 3564 2081
Fragments arranged by size:
3564 2081 47
StuI AGG'CCT
Cuts at: 1517 2342 2390 1517
Size: 825 48 4819
Fragments arranged by size:
4819 825 48
StyI C'CwwG_G
Cuts at: 513 1065 2004 2439 2586 2794 2891 2980 3131
Size: 552 939 435 147 208 97 89 151
Cuts at: 3131 513
Size: 3074
Fragments arranged by size:
3074 939 552 435 208 151 147 97
89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2609 3719 3736 3889 4074 4519 325
Size: 1255 1029 1110 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1110 1029 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3938 5650 96
Size: 274 80 33 51 1443 1961 1712 138
Fragments arranged by size:
1961 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2942 2974 5652 5684 2942
Size: 32 2678 32 2950
Fragments arranged by size:
2950 2678 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5692
XbaI T'CTAG_A
Cuts at: 3028 3028
Size: 5692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2362 2428 2011
Size: 351 66 5275
Fragments arranged by size:
5275 351 66
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5692
XmaI C'CCGG_G
Cuts at: 1870 2271 2374 3017 1870
Size: 401 103 643 4545
Fragments arranged by size:
4545 643 401 103
XmnI GAAnn'nnTTC
Cuts at: 3821 3821
Size: 5692
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3500 276
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BfrBI BsiWI BsmBI
BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FspAI
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI