Restriction map for
pFN21AE0754
(Circular) (Six-base) MAPSORT of: pFN21AE0754.seq Check: 3954 from: 1 to: 5995
pFN21AE0754 5995 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3805 278
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3338 1937
Size: 1401 4594
Acc65I G'GTAC_C
Cuts at: 3316 3316
Size: 5995
AclI AA'CG_TT
Cuts at: 1563 4122 4495 1563
Size: 2559 373 3063
Fragments arranged by size:
3063 2559 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2167 2627 4058 5442 5710 1632
Size: 535 460 1431 1384 268 1917
Fragments arranged by size:
1917 1431 1384 535 460 268
AfeI AGC'GCT
Cuts at: 2377 2377
Size: 5995
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5798
AflIII A'CryG_T
Cuts at: 1203 2588 4894 5871 1203
Size: 1385 2306 977 1327
Fragments arranged by size:
2306 1385 1327 977
AgeI A'CCGG_T
Cuts at: 2162 4826 2162
Size: 2664 3331
AhdI GACnn_n'nnGTC
Cuts at: 4724 4724
Size: 5995
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5995
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2325 1184
Size: 1141 4854
AlwNI CAG_nnn'CTG
Cuts at: 3023 3738 5310 3023
Size: 715 1572 3708
Fragments arranged by size:
3708 1572 715
ApaI G_GGCC'C
Cuts at: 2800 2800
Size: 5995
ApaLI G'TGCA_C
Cuts at: 4052 5208 4052
Size: 1156 4839
ApoI r'AATT_y
Cuts at: 1493 2406 2437 2517 2575 2712 3104 3118 3304
Size: 913 31 80 58 137 392 14 186
Cuts at: 3304 3530 3682 5791 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 913 392 226 186 152 137
80 58 31 14
AseI AT'TA_AT
Cuts at: 160 1032 2736 4549 160
Size: 872 1704 1813 1606
Fragments arranged by size:
1813 1704 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
AvaI C'yCGr_G
Cuts at: 1870 1943 3320 1870
Size: 73 1377 4545
Fragments arranged by size:
4545 1377 73
BamHI G'GATC_C
Cuts at: 3325 3692 3325
Size: 367 5628
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2159 3316 4771 618
Size: 325 217 627 372 1157 1455 1842
Fragments arranged by size:
1842 1455 1157 627 372 325 217
BanII G_rGCy'C
Cuts at: 1391 2199 2800 3314 1391
Size: 808 601 514 4072
Fragments arranged by size:
4072 808 601 514
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2103 2496 928
Size: 971 204 393 4427
Fragments arranged by size:
4427 971 393 204
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4186 4220 1903
Size: 34 2249 34 3678
Fragments arranged by size:
3678 2249 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3887 5097 3887
Size: 1210 4785
BclI T'GATC_A
Cuts at: 1548 2722 1548
Size: 1174 4821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4606 136
Size: 107 122 71 631 605 2934 1525
Fragments arranged by size:
2934 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5990 5990
Size: 5995
BlpI GC'TnA_GC
Cuts at: 3412 3412
Size: 5995
Bme1580I G_kGCm'C
Cuts at: 1790 2800 4056 5212 1790
Size: 1010 1256 1156 2573
Fragments arranged by size:
2573 1256 1156 1010
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5995
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3726 4684 442
Size: 416 53 83 399 24 2309 958 1753
Fragments arranged by size:
2309 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5995
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2849 2849
Size: 5995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 4655 1239
Size: 974 2442 2579
Fragments arranged by size:
2579 2442 974
Bpu10I CC'TnA_GC
Cuts at: 3153 3153
Size: 5995
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4120 4985 5283 4120
Size: 865 298 4832
Fragments arranged by size:
4832 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2837 4658 882
Size: 617 1338 1821 2219
Fragments arranged by size:
2219 1821 1338 617
BsaAI yAC'GTr
Cuts at: 493 1204 2218 3786 493
Size: 711 1014 1568 2702
Fragments arranged by size:
2702 1568 1014 711
BsaBI GATnn'nnATC
Cuts at: 5989 5989
Size: 5995
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2174 3268 3802 4184
Size: 53 83 186 936 641 1094 534 382
Cuts at: 4184 4819 275
Size: 635 1451
Fragments arranged by size:
1451 1094 936 641 635 534 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2162 4428 4826 5100 5247 5658 2162
Size: 2266 398 274 147 411 2499
Fragments arranged by size:
2499 2266 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5770
Fragments arranged by size:
5770 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2276 2774 1186
Size: 1090 498 4407
Fragments arranged by size:
4407 1090 498
BseYI C'CCAG_C
Cuts at: 5198 5694 5198
Size: 496 5499
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5422
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2167 3445 4206 5234 1540
Size: 275 186 166 1278 761 1028 2301
Fragments arranged by size:
2301 1278 1028 761 275 186 166
BsiHKAI G_wGCw'C
Cuts at: 3006 4056 4141 5212 3006
Size: 1050 85 1071 3789
Fragments arranged by size:
3789 1071 1050 85
BsmI GAATG_Cn'
Cuts at: 3506 3599 3506
Size: 93 5902
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2199 2800 3006 3314 4056 4141 5212
Size: 399 409 601 206 308 742 85 1071
Cuts at: 5212 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 601 409 399 308 206
85
BspEI T'CCGG_A
Cuts at: 5658 5658
Size: 5995
BspHI T'CATG_A
Cuts at: 3884 5614 3884
Size: 1730 4265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2317 3350 844
Size: 1473 1033 3489
Fragments arranged by size:
3489 1473 1033
BsrBI CCG'CTC
Cuts at: 1394 1823 3882 1394
Size: 429 2059 3507
Fragments arranged by size:
3507 2059 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4490 4664 65
Size: 1183 3242 174 1396
Fragments arranged by size:
3242 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2162 4639 4826 1700
Size: 110 352 2477 187 2869
Fragments arranged by size:
2869 2477 352 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5995
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5995
BssSI C'ACGA_G
Cuts at: 4055 5067 4055
Size: 1012 4983
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5995
BstBI TT'CG_AA
Cuts at: 4799 4799
Size: 5995
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5995
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2692 2011
Size: 681 5314
BstYI r'GATC_y
Cuts at: 1972 3325 3692 4077 4094 5546 5632 5644 5990
Size: 1353 367 385 17 1452 86 12 346
Cuts at: 5990 1972
Size: 1977
Fragments arranged by size:
1977 1452 1353 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4504
Fragments arranged by size:
4504 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5256
Fragments arranged by size:
5256 683 56
BtsI GCAGTG_nn'
Cuts at: 3511 4305 4325 3511
Size: 794 20 5181
Fragments arranged by size:
5181 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5995
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5960
DraI TTT'AAA
Cuts at: 2620 3109 3299 2620
Size: 489 190 5316
Fragments arranged by size:
5316 489 190
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2498 5002 809
Size: 507 1182 2504 1802
Fragments arranged by size:
2504 1802 1182 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2946 3442 4331
Size: 54 1425 50 275 18 1116 496 889
Cuts at: 4331 5960 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1116 889 496 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3442 1537
Size: 275 1630 4090
Fragments arranged by size:
4090 1630 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2027 2186 2532 3925 1353
Size: 674 159 346 1393 3423
Fragments arranged by size:
3423 1393 674 346 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2079 4582 4956 5102 387
Size: 1064 138 490 2503 374 146 1280
Fragments arranged by size:
2503 1280 1064 490 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2167 2213 2627 4058 4655 5442 5710
Size: 393 535 46 414 1431 597 787 268
Cuts at: 5710 1239
Size: 1524
Fragments arranged by size:
1524 1431 787 597 535 414 393 268
46
EcoO109I rG'GnC_Cy
Cuts at: 2796 2796
Size: 5995
EcoRI G'AATT_C
Cuts at: 3304 3682 3304
Size: 378 5617
FspI TGC'GCA
Cuts at: 4501 4501
Size: 5995
HaeII r_GCGC'y
Cuts at: 1678 2379 5142 1678
Size: 701 2763 2531
Fragments arranged by size:
2763 2531 701
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2822 2834 2849 2854 2866
Size: 32 135 32 1250 12 15 5 12
Cuts at: 2866 2881 4636 4668 4710 4742 1373
Size: 15 1755 32 42 32 2626
Fragments arranged by size:
2626 1755 1250 135 42 32 32 32
32 15 15 12 12 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3339 3585 669
Size: 988 281 1401 246 3079
Fragments arranged by size:
3079 1401 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3202 748
Size: 2454 3541
HpaI GTT'AAC
Cuts at: 3585 3585
Size: 5995
KpnI G_GTAC'C
Cuts at: 3320 3320
Size: 5995
MfeI C'AATT_G
Cuts at: 3594 3594
Size: 5995
MluI A'CGCG_T
Cuts at: 5871 5871
Size: 5995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5109 5293 1305
Size: 3804 184 2007
Fragments arranged by size:
3804 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4173
Fragments arranged by size:
4173 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2948 3953 4312 4471 518
Size: 648 1782 1005 359 159 2042
Fragments arranged by size:
2042 1782 1005 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3411 4088 5236 5481 1606
Size: 1805 677 1148 245 2120
Fragments arranged by size:
2120 1805 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4504
Fragments arranged by size:
4504 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5995
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5995
NotI GC'GGCC_GC
Cuts at: 3442 3442
Size: 5995
NspI r_CATG'y
Cuts at: 1132 1229 2592 3353 4898 1132
Size: 97 1363 761 1545 2229
Fragments arranged by size:
2229 1545 1363 761 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5995
PciI A'CATG_T
Cuts at: 2588 4894 2588
Size: 2306 3689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5995
PfoI T'CCnGG_A
Cuts at: 2049 2049
Size: 5995
PmeI GTTT'AAAC
Cuts at: 3299 3299
Size: 5995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4064 4096 5577 5609 4064
Size: 32 1481 32 4450
Fragments arranged by size:
4450 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5995
PsiI TTA'TAA
Cuts at: 2892 3565 2892
Size: 673 5322
PspOMI G'GGCC_C
Cuts at: 2796 2796
Size: 5995
PstI C_TGCA'G
Cuts at: 830 3187 3347 830
Size: 2357 160 3478
Fragments arranged by size:
3478 2357 160
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5995
SalI G'TCGA_C
Cuts at: 1936 3337 1936
Size: 1401 4594
SbfI CC_TGCA'GG
Cuts at: 3347 3347
Size: 5995
ScaI AGT'ACT
Cuts at: 4243 5955 4243
Size: 1712 4283
SexAI A'CCwGG_T
Cuts at: 2446 2446
Size: 5995
SfcI C'TryA_G
Cuts at: 826 1045 2101 3183 3343 4478 5159 5350 826
Size: 219 1056 1082 160 1135 681 191 1471
Fragments arranged by size:
1471 1135 1082 1056 681 219 191 160
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5995
SmaI CCC'GGG
Cuts at: 1872 3322 1872
Size: 1450 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4099 5000 5262 820
Size: 197 926 2156 901 262 1553
Fragments arranged by size:
2156 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3786 493
Size: 3293 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5995
SphI G_CATG'C
Cuts at: 1132 3353 1132
Size: 2221 3774
SspI AAT'ATT
Cuts at: 5 52 2734 3919 5
Size: 47 2682 1185 2081
Fragments arranged by size:
2682 2081 1185 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5995
StyI C'CwwG_G
Cuts at: 513 1065 2004 2010 3434 513
Size: 552 939 6 1424 3074
Fragments arranged by size:
3074 1424 939 552 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4022 4039 4192 4377 4822 325
Size: 1255 2442 17 153 185 445 1498
Fragments arranged by size:
2442 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2273 4241 5953
Size: 274 80 33 51 1443 296 1968 1712
Cuts at: 5953 96
Size: 138
Fragments arranged by size:
1968 1712 1443 296 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5955 5987 5955
Size: 32 5963
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5995
XbaI T'CTAG_A
Cuts at: 2852 3331 2852
Size: 479 5516
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2153 2807 2153
Size: 654 5341
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5995
XmaI C'CCGG_G
Cuts at: 1870 3320 1870
Size: 1450 4545
XmnI GAAnn'nnTTC
Cuts at: 2406 4124 2406
Size: 1718 4277
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3803 276
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI