Restriction map for
pFN21AE0750
(Circular) (Six-base) MAPSORT of: pFN21AE0750.seq Check: 5143 from: 1 to: 5983
pFN21AE0750 5983 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3793 278
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3326 1937
Size: 1389 4594
Acc65I G'GTAC_C
Cuts at: 3304 3304
Size: 5983
AclI AA'CG_TT
Cuts at: 1563 4110 4483 1563
Size: 2547 373 3063
Fragments arranged by size:
3063 2547 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2250 2291 2389 4046 5430 5698 1632
Size: 618 41 98 1657 1384 268 1917
Fragments arranged by size:
1917 1657 1384 618 268 98 41
AflII C'TTAA_G
Cuts at: 820 1017 2423 820
Size: 197 1406 4380
Fragments arranged by size:
4380 1406 197
AflIII A'CryG_T
Cuts at: 1203 3004 4882 5859 1203
Size: 1801 1878 977 1327
Fragments arranged by size:
1878 1801 1327 977
AgeI A'CCGG_T
Cuts at: 2100 4814 2100
Size: 2714 3269
AhdI GACnn_n'nnGTC
Cuts at: 2101 4712 2101
Size: 2611 3372
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5983
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2513 1184
Size: 1329 4654
AlwNI CAG_nnn'CTG
Cuts at: 3726 5298 3726
Size: 1572 4411
ApaLI G'TGCA_C
Cuts at: 2498 4040 5196 2498
Size: 1542 1156 3285
Fragments arranged by size:
3285 1542 1156
ApoI r'AATT_y
Cuts at: 1493 2107 2534 2592 2702 2899 2928 3180 3292
Size: 614 427 58 110 197 29 252 112
Cuts at: 3292 3518 3670 5779 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 614 427 252 226 197 152
112 110 58 29
AseI AT'TA_AT
Cuts at: 160 1032 4537 160
Size: 872 3505 1606
Fragments arranged by size:
3505 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
AvaI C'yCGr_G
Cuts at: 1870 1943 3308 1870
Size: 73 1365 4545
Fragments arranged by size:
4545 1365 73
BamHI G'GATC_C
Cuts at: 3313 3680 3313
Size: 367 5616
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3304 4759 618
Size: 325 217 627 1517 1455 1842
Fragments arranged by size:
1842 1517 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2443 3302 1391
Size: 1052 859 4072
Fragments arranged by size:
4072 1052 859
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2395 2548 2611 3230 928
Size: 971 496 153 63 619 3681
Fragments arranged by size:
3681 971 619 496 153 63
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4174 4208 1903
Size: 34 2237 34 3678
Fragments arranged by size:
3678 2237 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2337 3875 5085 2337
Size: 1538 1210 3235
Fragments arranged by size:
3235 1538 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5983
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4594 136
Size: 107 122 71 631 605 2922 1525
Fragments arranged by size:
2922 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3152 5978 3152
Size: 2826 3157
BlpI GC'TnA_GC
Cuts at: 3400 3400
Size: 5983
Bme1580I G_kGCm'C
Cuts at: 1790 2502 4044 5200 1790
Size: 712 1542 1156 2573
Fragments arranged by size:
2573 1542 1156 712
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5983
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2349 3714 4672
Size: 416 53 83 399 24 932 1365 958
Cuts at: 4672 442
Size: 1753
Fragments arranged by size:
1753 1365 958 932 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5983
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2711 2711
Size: 5983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2237 2553 3126 4643 1239
Size: 998 316 573 1517 2579
Fragments arranged by size:
2579 1517 998 573 316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4108 4973 5271 4108
Size: 865 298 4820
Fragments arranged by size:
4820 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4646 882
Size: 617 3147 2219
Fragments arranged by size:
3147 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3774 493
Size: 711 2570 2702
Fragments arranged by size:
2702 2570 711
BsaBI GATnn'nnATC
Cuts at: 5977 5977
Size: 5983
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3790 4172 4807 275
Size: 53 83 186 936 2257 382 635 1451
Fragments arranged by size:
2257 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2100 4416 4814 5088 5235 5646 2100
Size: 2316 398 274 147 411 2437
Fragments arranged by size:
2437 2316 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2551 2581 3267 3297 1178
Size: 30 165 30 1148 30 686 30 3864
Fragments arranged by size:
3864 1148 686 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 1186
Size: 991 4992
BseYI C'CCAG_C
Cuts at: 5186 5682 5186
Size: 496 5487
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2817 1163
Size: 573 1081 4329
Fragments arranged by size:
4329 1081 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3433 4194 5222 1540
Size: 275 186 1432 761 1028 2301
Fragments arranged by size:
2301 1432 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2443 2502 4044 4129 5200 2443
Size: 59 1542 85 1071 3226
Fragments arranged by size:
3226 1542 1071 85 59
BsmI GAATG_Cn'
Cuts at: 3494 3587 3494
Size: 93 5890
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2443 2502 3302 4044 4129 5200 1391
Size: 399 653 59 800 742 85 1071 2174
Fragments arranged by size:
2174 1071 800 742 653 399 85 59
BspEI T'CCGG_A
Cuts at: 5646 5646
Size: 5983
BspHI T'CATG_A
Cuts at: 3872 5602 3872
Size: 1730 4253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3338 844
Size: 2494 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3870 1394
Size: 429 2047 3507
Fragments arranged by size:
3507 2047 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4478 4652 65
Size: 1183 3230 174 1396
Fragments arranged by size:
3230 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2100 4627 4814 1700
Size: 110 290 2527 187 2869
Fragments arranged by size:
2869 2527 290 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5983
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5983
BssSI C'ACGA_G
Cuts at: 4043 5055 4043
Size: 1012 4971
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5983
BstBI TT'CG_AA
Cuts at: 4787 4787
Size: 5983
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5983
BstXI CCAn_nnnn'nTGG
Cuts at: 2166 2166
Size: 5983
BstYI r'GATC_y
Cuts at: 1972 2888 3152 3313 3680 4065 4082 5534 5620
Size: 916 264 161 367 385 17 1452 86
Cuts at: 5620 5632 5978 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 916 385 367 346 264 161
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2026 513
Size: 552 961 4470
Fragments arranged by size:
4470 961 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5244
Fragments arranged by size:
5244 683 56
BtsI GCAGTG_nn'
Cuts at: 2756 3499 4293 4313 2756
Size: 743 794 20 4426
Fragments arranged by size:
4426 794 743 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5983
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5948
DraI TTT'AAA
Cuts at: 3287 3287
Size: 5983
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4990 809
Size: 507 3674 1802
Fragments arranged by size:
3674 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3052 3430 4319
Size: 54 1425 50 275 18 1222 378 889
Cuts at: 4319 5948 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1222 889 378 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3430 1537
Size: 275 1618 4090
Fragments arranged by size:
4090 1618 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3913 1353
Size: 2560 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4570 4944 5090 387
Size: 1064 138 2981 374 146 1280
Fragments arranged by size:
2981 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2441 2441
Size: 5983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2250 2291 2389 2553 3126 4046
Size: 393 605 13 41 98 164 573 920
Cuts at: 4046 4643 5430 5698 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 920 787 605 597 573 393 268
164 98 41 13
EcoRI G'AATT_C
Cuts at: 3180 3292 3670 3180
Size: 112 378 5493
Fragments arranged by size:
5493 378 112
FspI TGC'GCA
Cuts at: 4489 4489
Size: 5983
HaeII r_GCGC'y
Cuts at: 1678 5130 1678
Size: 3452 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2087 2119 2364 2396 4624
Size: 32 135 32 515 32 245 32 2228
Cuts at: 4624 4656 4698 4730 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2228 515 245 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3327 3573 669
Size: 988 281 1389 246 3079
Fragments arranged by size:
3079 1389 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5983
HpaI GTT'AAC
Cuts at: 3573 3573
Size: 5983
KpnI G_GTAC'C
Cuts at: 3308 3308
Size: 5983
MfeI C'AATT_G
Cuts at: 3582 3582
Size: 5983
MluI A'CGCG_T
Cuts at: 5859 5859
Size: 5983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5097 5281 1305
Size: 3792 184 2007
Fragments arranged by size:
3792 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3054 10
Size: 54 1425 343 1222 2939
Fragments arranged by size:
2939 1425 1222 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2495 2953 3941 4300 4459 518
Size: 648 1329 458 988 359 159 2042
Fragments arranged by size:
2042 1329 988 648 458 359 159
MspA1I CmG'CkG
Cuts at: 1606 3399 4076 5224 5469 1606
Size: 1793 677 1148 245 2120
Fragments arranged by size:
2120 1793 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2026 513
Size: 552 961 4470
Fragments arranged by size:
4470 961 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5983
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5983
NotI GC'GGCC_GC
Cuts at: 3430 3430
Size: 5983
NspI r_CATG'y
Cuts at: 1132 1229 2840 3008 3341 4886 1132
Size: 97 1611 168 333 1545 2229
Fragments arranged by size:
2229 1611 1545 333 168 97
PasI CC'CwG_GG
Cuts at: 1348 2040 1348
Size: 692 5291
PciI A'CATG_T
Cuts at: 3004 4882 3004
Size: 1878 4105
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5983
PmeI GTTT'AAAC
Cuts at: 3287 3287
Size: 5983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2222 2254 4052 4084 5565 5597 2222
Size: 32 1798 32 1481 32 2608
Fragments arranged by size:
2608 1798 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5983
PsiI TTA'TAA
Cuts at: 3553 3553
Size: 5983
PstI C_TGCA'G
Cuts at: 830 3335 830
Size: 2505 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5983
SacI G_AGCT'C
Cuts at: 2443 2443
Size: 5983
SalI G'TCGA_C
Cuts at: 1936 3325 1936
Size: 1389 4594
SbfI CC_TGCA'GG
Cuts at: 3335 3335
Size: 5983
ScaI AGT'ACT
Cuts at: 4231 5943 4231
Size: 1712 4271
SfcI C'TryA_G
Cuts at: 826 1045 2200 2365 3331 4466 5147 5338 826
Size: 219 1155 165 966 1135 681 191 1471
Fragments arranged by size:
1471 1155 1135 966 681 219 191 165
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5983
SmaI CCC'GGG
Cuts at: 1872 3310 1872
Size: 1438 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2423 4087 4988 5250 820
Size: 197 926 480 1664 901 262 1553
Fragments arranged by size:
1664 1553 926 901 480 262 197
SnaBI TAC'GTA
Cuts at: 493 3774 493
Size: 3281 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5983
SphI G_CATG'C
Cuts at: 1132 3341 1132
Size: 2209 3774
SspI AAT'ATT
Cuts at: 5 52 2334 3907 5
Size: 47 2282 1573 2081
Fragments arranged by size:
2282 2081 1573 47
StuI AGG'CCT
Cuts at: 1517 2574 1517
Size: 1057 4926
StyI C'CwwG_G
Cuts at: 513 1065 2026 3422 513
Size: 552 961 1396 3074
Fragments arranged by size:
3074 1396 961 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4010 4027 4180 4365 4810 325
Size: 1255 2430 17 153 185 445 1498
Fragments arranged by size:
2430 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3069 4229 5941
Size: 274 80 33 51 1443 1092 1160 1712
Cuts at: 5941 96
Size: 138
Fragments arranged by size:
1712 1443 1160 1092 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2368 2400 2551 2583 5943 5975 2368
Size: 32 151 32 3360 32 2376
Fragments arranged by size:
3360 2376 151 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5983
XbaI T'CTAG_A
Cuts at: 3319 3319
Size: 5983
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5983
XmaI C'CCGG_G
Cuts at: 1870 3308 1870
Size: 1438 4545
XmnI GAAnn'nnTTC
Cuts at: 2807 2975 3143 3227 4112 2807
Size: 168 168 84 885 4678
Fragments arranged by size:
4678 885 168 168 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3791 276
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI