Restriction map for
pFN21AE0740
(Circular) (Six-base) MAPSORT of: pFN21AE0740.seq Check: 4757 from: 1 to: 5977
pFN21AE0740 5977 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3787 278
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3320 1937
Size: 1383 4594
Acc65I G'GTAC_C
Cuts at: 3298 3298
Size: 5977
AclI AA'CG_TT
Cuts at: 1563 4104 4477 1563
Size: 2541 373 3063
Fragments arranged by size:
3063 2541 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2328 2482 2902 4040 5424 5692 1632
Size: 696 154 420 1138 1384 268 1917
Fragments arranged by size:
1917 1384 1138 696 420 268 154
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5780
AflIII A'CryG_T
Cuts at: 1203 2726 4876 5853 1203
Size: 1523 2150 977 1327
Fragments arranged by size:
2150 1523 1327 977
AgeI A'CCGG_T
Cuts at: 4808 4808
Size: 5977
AhdI GACnn_n'nnGTC
Cuts at: 4706 4706
Size: 5977
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5977
AlwNI CAG_nnn'CTG
Cuts at: 3720 5292 3720
Size: 1572 4405
ApaLI G'TGCA_C
Cuts at: 4034 5190 4034
Size: 1156 4821
ApoI r'AATT_y
Cuts at: 1493 2433 2532 3014 3229 3286 3512 3664 5773
Size: 940 99 482 215 57 226 152 2109
Cuts at: 5773 1493
Size: 1697
Fragments arranged by size:
2109 1697 940 482 226 215 152 99
57
AseI AT'TA_AT
Cuts at: 160 1032 2187 4531 160
Size: 872 1155 2344 1606
Fragments arranged by size:
2344 1606 1155 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
AvaI C'yCGr_G
Cuts at: 1870 1943 2027 3302 1870
Size: 73 84 1275 4545
Fragments arranged by size:
4545 1275 84 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2859 2892 2859
Size: 33 5944
BamHI G'GATC_C
Cuts at: 3307 3674 3307
Size: 367 5610
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3298 4753 618
Size: 325 217 627 1511 1455 1842
Fragments arranged by size:
1842 1511 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3296 1391
Size: 1905 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5006
BbvCI CC'TCA_GC
Cuts at: 2117 2117
Size: 5977
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4168 4202 1903
Size: 34 2231 34 3678
Fragments arranged by size:
3678 2231 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3869 5079 3869
Size: 1210 4767
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5977
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4588 136
Size: 107 122 71 631 605 2916 1525
Fragments arranged by size:
2916 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2577 5972 2577
Size: 3395 2582
BlpI GC'TnA_GC
Cuts at: 3394 3394
Size: 5977
Bme1580I G_kGCm'C
Cuts at: 1790 4038 5194 1790
Size: 2248 1156 2573
Fragments arranged by size:
2573 2248 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5977
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2881 3708 4666
Size: 416 53 83 399 24 1464 827 958
Cuts at: 4666 442
Size: 1753
Fragments arranged by size:
1753 1464 958 827 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3185 1056
Size: 2129 3848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4637 1239
Size: 3398 2579
Bpu10I CC'TnA_GC
Cuts at: 2117 2117
Size: 5977
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2061 4102 4967 5265 2061
Size: 2041 865 298 2773
Fragments arranged by size:
2773 2041 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4640 882
Size: 617 3141 2219
Fragments arranged by size:
3141 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2707 3768 493
Size: 711 1503 1061 2702
Fragments arranged by size:
2702 1503 1061 711
BsaBI GATnn'nnATC
Cuts at: 5971 5971
Size: 5977
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2024 3784 4166
Size: 53 83 186 936 476 15 1760 382
Cuts at: 4166 4801 275
Size: 635 1451
Fragments arranged by size:
1760 1451 936 635 476 382 186 83
53 15
BsaWI w'CCGG_w
Cuts at: 4410 4808 5082 5229 5640 4410
Size: 398 274 147 411 4747
Fragments arranged by size:
4747 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5752
Fragments arranged by size:
5752 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 1186
Size: 910 5067
BseYI C'CCAG_C
Cuts at: 5180 5676 5180
Size: 496 5481
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2081 2238 2674 1163
Size: 573 345 157 436 4466
Fragments arranged by size:
4466 573 436 345 157
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3427 4188 5216 1540
Size: 275 186 1426 761 1028 2301
Fragments arranged by size:
2301 1426 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4038 4123 5194 4038
Size: 85 1071 4821
Fragments arranged by size:
4821 1071 85
BsmI GAATG_Cn'
Cuts at: 3488 3581 3488
Size: 93 5884
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3296 4038 4123 5194 1391
Size: 399 1506 742 85 1071 2174
Fragments arranged by size:
2174 1506 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5640 5640
Size: 5977
BspHI T'CATG_A
Cuts at: 3866 5596 3866
Size: 1730 4247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3332 844
Size: 2488 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3864 1394
Size: 429 2041 3507
Fragments arranged by size:
3507 2041 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2247 4472 4646 65
Size: 1183 999 2225 174 1396
Fragments arranged by size:
2225 1396 1183 999 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4621 4808 1700
Size: 110 2811 187 2869
Fragments arranged by size:
2869 2811 187 110
BsrGI T'GTAC_A
Cuts at: 96 2469 96
Size: 2373 3604
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5977
BssSI C'ACGA_G
Cuts at: 4037 5049 4037
Size: 1012 4965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3123 794
Size: 2329 3648
BstBI TT'CG_AA
Cuts at: 4781 4781
Size: 5977
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5977
BstYI r'GATC_y
Cuts at: 1972 2056 2577 3307 3674 4059 4076 5528 5614
Size: 84 521 730 367 385 17 1452 86
Cuts at: 5614 5626 5972 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 730 521 385 367 346 86
84 17 12
Bsu36I CC'TnA_GG
Cuts at: 2600 2862 2600
Size: 262 5715
BtgI C'CryG_G
Cuts at: 513 1065 2004 3273 513
Size: 552 939 1269 3217
Fragments arranged by size:
3217 1269 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2997 487
Size: 683 56 1771 3467
Fragments arranged by size:
3467 1771 683 56
BtsI GCAGTG_nn'
Cuts at: 2662 3493 4287 4307 2662
Size: 831 794 20 4332
Fragments arranged by size:
4332 831 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5942
DraI TTT'AAA
Cuts at: 3196 3281 3196
Size: 85 5892
DraIII CAC_nnn'GTG
Cuts at: 2904 2904
Size: 5977
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4984 809
Size: 507 3668 1802
Fragments arranged by size:
3668 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3424 4313 5942
Size: 54 1425 50 275 18 1594 889 1629
Cuts at: 5942 8
Size: 43
Fragments arranged by size:
1629 1594 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3424 1537
Size: 275 1612 4090
Fragments arranged by size:
4090 1612 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2906 2945 3164 3907 1353
Size: 1553 39 219 743 3423
Fragments arranged by size:
3423 1553 743 219 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4564 4938 5084 387
Size: 1064 138 2975 374 146 1280
Fragments arranged by size:
2975 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2328 2482 2902 4040 4637 5424 5692
Size: 393 696 154 420 1138 597 787 268
Cuts at: 5692 1239
Size: 1524
Fragments arranged by size:
1524 1138 787 696 597 420 393 268
154
EcoO109I rG'GnC_Cy
Cuts at: 2112 2882 3105 2112
Size: 770 223 4984
Fragments arranged by size:
4984 770 223
EcoRI G'AATT_C
Cuts at: 3286 3664 3286
Size: 378 5599
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3156 3156
Size: 5977
FspI TGC'GCA
Cuts at: 4483 4483
Size: 5977
HaeII r_GCGC'y
Cuts at: 1678 5124 1678
Size: 3446 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2569 2601 2951 2983 4618
Size: 32 135 32 997 32 350 32 1635
Cuts at: 4618 4650 4692 4724 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1635 997 350 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3321 3567 669
Size: 988 281 1383 246 3079
Fragments arranged by size:
3079 1383 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5977
HpaI GTT'AAC
Cuts at: 3567 3567
Size: 5977
KpnI G_GTAC'C
Cuts at: 3302 3302
Size: 5977
MfeI C'AATT_G
Cuts at: 3576 3576
Size: 5977
MluI A'CGCG_T
Cuts at: 5853 5853
Size: 5977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2278 5091 5275 1305
Size: 973 2813 184 2007
Fragments arranged by size:
2813 2007 973 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4155
Fragments arranged by size:
4155 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2559 2625 2835 3935 4294 4453 518
Size: 648 1393 66 210 1100 359 159 2042
Fragments arranged by size:
2042 1393 1100 648 359 210 159 66
MspA1I CmG'CkG
Cuts at: 1606 3393 4070 5218 5463 1606
Size: 1787 677 1148 245 2120
Fragments arranged by size:
2120 1787 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4486
Fragments arranged by size:
4486 939 552
NdeI CA'TA_TG
Cuts at: 387 2735 387
Size: 2348 3629
NheI G'CTAG_C
Cuts at: 1052 3181 1052
Size: 2129 3848
NotI GC'GGCC_GC
Cuts at: 3424 3424
Size: 5977
NspI r_CATG'y
Cuts at: 1132 1229 2730 3126 3162 3181 3335 4880 1132
Size: 97 1501 396 36 19 154 1545 2229
Fragments arranged by size:
2229 1545 1501 396 154 97 36 19
PasI CC'CwG_GG
Cuts at: 1348 2035 2448 1348
Size: 687 413 4877
Fragments arranged by size:
4877 687 413
PciI A'CATG_T
Cuts at: 2726 4876 2726
Size: 2150 3827
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5977
PmeI GTTT'AAAC
Cuts at: 3281 3281
Size: 5977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4046 4078 5559 5591 4046
Size: 32 1481 32 4432
Fragments arranged by size:
4432 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2112 2112
Size: 5977
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5977
PsiI TTA'TAA
Cuts at: 2640 3547 2640
Size: 907 5070
PstI C_TGCA'G
Cuts at: 830 2981 3329 830
Size: 2151 348 3478
Fragments arranged by size:
3478 2151 348
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5977
SalI G'TCGA_C
Cuts at: 1936 3319 1936
Size: 1383 4594
SapI GCTCTTCn'nnn_
Cuts at: 2906 2945 3164 2906
Size: 39 219 5719
Fragments arranged by size:
5719 219 39
SbfI CC_TGCA'GG
Cuts at: 3329 3329
Size: 5977
ScaI AGT'ACT
Cuts at: 4225 5937 4225
Size: 1712 4265
SfcI C'TryA_G
Cuts at: 826 1045 2977 3325 4460 5141 5332 826
Size: 219 1932 348 1135 681 191 1471
Fragments arranged by size:
1932 1471 1135 681 348 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5977
SmaI CCC'GGG
Cuts at: 1872 3304 1872
Size: 1432 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2076 4081 4982 5244 820
Size: 197 926 133 2005 901 262 1553
Fragments arranged by size:
2005 1553 926 901 262 197 133
SnaBI TAC'GTA
Cuts at: 493 3768 493
Size: 3275 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5977
SphI G_CATG'C
Cuts at: 1132 3162 3335 1132
Size: 2030 173 3774
Fragments arranged by size:
3774 2030 173
SspI AAT'ATT
Cuts at: 5 52 2319 2408 3901 5
Size: 47 2267 89 1493 2081
Fragments arranged by size:
2267 2081 1493 89 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5977
StyI C'CwwG_G
Cuts at: 513 1065 2004 3416 513
Size: 552 939 1412 3074
Fragments arranged by size:
3074 1412 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4004 4021 4174 4359 4804 325
Size: 1255 2424 17 153 185 445 1498
Fragments arranged by size:
2424 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2469 4223 5935
Size: 274 80 33 51 1443 492 1754 1712
Cuts at: 5935 96
Size: 138
Fragments arranged by size:
1754 1712 1443 492 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2247 2279 5937 5969 2247
Size: 32 3658 32 2255
Fragments arranged by size:
3658 2255 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5977
XbaI T'CTAG_A
Cuts at: 3313 3313
Size: 5977
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5977
XmaI C'CCGG_G
Cuts at: 1870 3302 1870
Size: 1432 4545
XmnI GAAnn'nnTTC
Cuts at: 2915 3218 4106 2915
Size: 303 888 4786
Fragments arranged by size:
4786 888 303
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3785 276
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI