Restriction map for
pFN21AE0738
(Circular) (Six-base) MAPSORT of: pFN21AE0738.seq Check: 4539 from: 1 to: 5977
pFN21AE0738 5977 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2335 2335
Size: 5977
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3787 278
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3320 1937
Size: 1383 4594
Acc65I G'GTAC_C
Cuts at: 3298 3298
Size: 5977
AclI AA'CG_TT
Cuts at: 1563 4104 4477 1563
Size: 2541 373 3063
Fragments arranged by size:
3063 2541 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2479 2881 3234 4040 5424 5692 1632
Size: 847 402 353 806 1384 268 1917
Fragments arranged by size:
1917 1384 847 806 402 353 268
AfeI AGC'GCT
Cuts at: 2201 2201
Size: 5977
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5780
AflIII A'CryG_T
Cuts at: 1203 4876 5853 1203
Size: 3673 977 1327
Fragments arranged by size:
3673 1327 977
AgeI A'CCGG_T
Cuts at: 4808 4808
Size: 5977
AhdI GACnn_n'nnGTC
Cuts at: 4706 4706
Size: 5977
AleI CACnn'nnGTG
Cuts at: 1166 2954 1166
Size: 1788 4189
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5977
AlwNI CAG_nnn'CTG
Cuts at: 2269 2281 3000 3720 5292 2269
Size: 12 719 720 1572 2954
Fragments arranged by size:
2954 1572 720 719 12
ApaI G_GGCC'C
Cuts at: 2312 2312
Size: 5977
ApaLI G'TGCA_C
Cuts at: 4034 5190 4034
Size: 1156 4821
ApoI r'AATT_y
Cuts at: 1493 3286 3512 3664 5773 1493
Size: 1793 226 152 2109 1697
Fragments arranged by size:
2109 1793 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4531 160
Size: 872 3499 1606
Fragments arranged by size:
3499 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
AvaI C'yCGr_G
Cuts at: 1870 1943 2043 2781 2796 3121 3302 1870
Size: 73 100 738 15 325 181 4545
Fragments arranged by size:
4545 738 325 181 100 73 15
BamHI G'GATC_C
Cuts at: 3307 3674 3307
Size: 367 5610
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 3254 3298 4753 618
Size: 325 217 627 261 1206 44 1455 1842
Fragments arranged by size:
1842 1455 1206 627 325 261 217 44
BanII G_rGCy'C
Cuts at: 1391 2312 2546 2890 3296 1391
Size: 921 234 344 406 4072
Fragments arranged by size:
4072 921 406 344 234
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2503 928
Size: 971 604 4402
Fragments arranged by size:
4402 971 604
BbvCI CC'TCA_GC
Cuts at: 3170 3176 3182 3170
Size: 6 6 5965
Fragments arranged by size:
5965 6 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4168 4202 1903
Size: 34 2231 34 3678
Fragments arranged by size:
3678 2231 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3869 5079 3869
Size: 1210 4767
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5977
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2190 4588 136
Size: 107 122 71 631 605 518 2398 1525
Fragments arranged by size:
2398 1525 631 605 518 122 107 71
BglII A'GATC_T
Cuts at: 2679 5972 2679
Size: 3293 2684
BlpI GC'TnA_GC
Cuts at: 2351 3394 2351
Size: 1043 4934
Bme1580I G_kGCm'C
Cuts at: 1790 2051 2312 4038 5194 1790
Size: 261 261 1726 1156 2573
Fragments arranged by size:
2573 1726 1156 261 261
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5977
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3708 4666 442
Size: 416 53 83 399 24 2291 958 1753
Fragments arranged by size:
2291 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2417 2755 3136 4637 1239
Size: 1178 338 381 1501 2579
Fragments arranged by size:
2579 1501 1178 381 338
Bpu10I CC'TnA_GC
Cuts at: 2545 2767 3170 3176 3182 2545
Size: 222 403 6 6 5340
Fragments arranged by size:
5340 403 222 6 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2490 4102 4967 5265 2490
Size: 1612 865 298 3202
Fragments arranged by size:
3202 1612 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3257 4640 882
Size: 617 1758 1383 2219
Fragments arranged by size:
2219 1758 1383 617
BsaAI yAC'GTr
Cuts at: 493 1204 3768 493
Size: 711 2564 2702
Fragments arranged by size:
2702 2564 711
BsaBI GATnn'nnATC
Cuts at: 5971 5971
Size: 5977
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3784 4166 4801 275
Size: 53 83 186 936 2251 382 635 1451
Fragments arranged by size:
2251 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4410 4808 5082 5229 5640 4410
Size: 398 274 147 411 4747
Fragments arranged by size:
4747 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5752
Fragments arranged by size:
5752 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2111 2739 1186
Size: 925 628 4424
Fragments arranged by size:
4424 925 628
BseYI C'CCAG_C
Cuts at: 2083 2188 2562 2883 3081 5180 5676 2083
Size: 105 374 321 198 2099 496 2384
Fragments arranged by size:
2384 2099 496 374 321 198 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2210 2344 1163
Size: 573 474 134 4796
Fragments arranged by size:
4796 573 474 134
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2151 3427 4188 5216 1540
Size: 275 186 150 1276 761 1028 2301
Fragments arranged by size:
2301 1276 1028 761 275 186 150
BsiHKAI G_wGCw'C
Cuts at: 2352 4038 4123 5194 2352
Size: 1686 85 1071 3135
Fragments arranged by size:
3135 1686 1071 85
BsmI GAATG_Cn'
Cuts at: 2935 3488 3581 2935
Size: 553 93 5331
Fragments arranged by size:
5331 553 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2312 2352 2546 2890 3296 4038
Size: 399 261 261 40 194 344 406 742
Cuts at: 4038 4123 5194 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 406 399 344 261 261
194 85 40
BspEI T'CCGG_A
Cuts at: 5640 5640
Size: 5977
BspHI T'CATG_A
Cuts at: 3866 5596 3866
Size: 1730 4247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2335 2349 2466 3332 844
Size: 1491 14 117 866 3489
Fragments arranged by size:
3489 1491 866 117 14
BsrBI CCG'CTC
Cuts at: 1394 1823 3150 3864 1394
Size: 429 1327 714 3507
Fragments arranged by size:
3507 1327 714 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4472 4646 65
Size: 1183 3224 174 1396
Fragments arranged by size:
3224 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2635 3096 4621 4808 1700
Size: 110 825 461 1525 187 2869
Fragments arranged by size:
2869 1525 825 461 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5977
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5977
BssSI C'ACGA_G
Cuts at: 4037 5049 4037
Size: 1012 4965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5977
BstBI TT'CG_AA
Cuts at: 4781 4781
Size: 5977
BstEII G'GTnAC_C
Cuts at: 1184 2638 1184
Size: 1454 4523
BstXI CCAn_nnnn'nTGG
Cuts at: 2137 2392 2956 2137
Size: 255 564 5158
Fragments arranged by size:
5158 564 255
BstYI r'GATC_y
Cuts at: 1972 2679 2832 3012 3307 3674 4059 4076 5528
Size: 707 153 180 295 367 385 17 1452
Cuts at: 5528 5614 5626 5972 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 707 385 367 346 295 180
153 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2250 2591 3044 513
Size: 552 939 246 341 453 3446
Fragments arranged by size:
3446 939 552 453 341 246
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2449 2860 487
Size: 683 56 1223 411 3604
Fragments arranged by size:
3604 1223 683 411 56
BtsI GCAGTG_nn'
Cuts at: 3493 4287 4307 3493
Size: 794 20 5163
Fragments arranged by size:
5163 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5942
DraI TTT'AAA
Cuts at: 3281 3281
Size: 5977
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4984 809
Size: 507 3668 1802
Fragments arranged by size:
3668 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2103 2127 2247
Size: 54 1425 50 275 18 273 24 120
Cuts at: 2247 2382 2484 3162 3424 4313 5942 8
Size: 135 102 678 262 889 1629 43
Fragments arranged by size:
1629 1425 889 678 275 273 262 135
120 102 54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3424 1537
Size: 275 1612 4090
Fragments arranged by size:
4090 1612 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2264 3210 3907 1353
Size: 911 946 697 3423
Fragments arranged by size:
3423 946 911 697
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4564 4938 5084 387
Size: 1064 138 2975 374 146 1280
Fragments arranged by size:
2975 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2417 2479 2755 2881 3136 3234 4040
Size: 393 785 62 276 126 255 98 806
Cuts at: 4040 4637 5424 5692 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 806 787 785 597 393 276 268
255 126 98 62
EcoNI CCTnn'n_nnAGG
Cuts at: 2528 2528
Size: 5977
EcoO109I rG'GnC_Cy
Cuts at: 2308 2309 2751 3077 2308
Size: 1 442 326 5208
Fragments arranged by size:
5208 442 326 1
EcoRI G'AATT_C
Cuts at: 3286 3664 3286
Size: 378 5599
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2869 2869
Size: 5977
FspI TGC'GCA
Cuts at: 2426 4483 2426
Size: 2057 3920
HaeII r_GCGC'y
Cuts at: 1678 2203 2258 5124 1678
Size: 525 55 2866 2531
Fragments arranged by size:
2866 2531 525 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2738 2770 4618 4650 4692
Size: 32 135 32 1166 32 1848 32 42
Cuts at: 4692 4724 1373
Size: 32 2626
Fragments arranged by size:
2626 1848 1166 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3321 3567 669
Size: 988 281 1383 246 3079
Fragments arranged by size:
3079 1383 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5977
HpaI GTT'AAC
Cuts at: 3567 3567
Size: 5977
KpnI G_GTAC'C
Cuts at: 3302 3302
Size: 5977
MfeI C'AATT_G
Cuts at: 3576 3576
Size: 5977
MluI A'CGCG_T
Cuts at: 5853 5853
Size: 5977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3132 5091 5275 1305
Size: 1827 1959 184 2007
Fragments arranged by size:
2007 1959 1827 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2105 2129 2384 10
Size: 54 1425 343 273 24 255 3603
Fragments arranged by size:
3603 1425 343 273 255 54 24
MslI CAynn'nnrTG
Cuts at: 518 1166 2372 2954 2972 3935 4294 4453 518
Size: 648 1206 582 18 963 359 159 2042
Fragments arranged by size:
2042 1206 963 648 582 359 159 18
MspA1I CmG'CkG
Cuts at: 1606 2188 2278 2997 3046 3156 3260 3393 4070
Size: 582 90 719 49 110 104 133 677
Cuts at: 4070 5218 5463 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 719 677 582 245 133 110
104 90 49
NaeI GCC'GGC
Cuts at: 3098 3098
Size: 5977
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4486
Fragments arranged by size:
4486 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5977
NgoMIV G'CCGG_C
Cuts at: 3096 3096
Size: 5977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5977
NotI GC'GGCC_GC
Cuts at: 3424 3424
Size: 5977
NspI r_CATG'y
Cuts at: 1132 1229 2212 2359 2377 3335 4880 1132
Size: 97 983 147 18 958 1545 2229
Fragments arranged by size:
2229 1545 983 958 147 97 18
PasI CC'CwG_GG
Cuts at: 1348 2179 1348
Size: 831 5146
PciI A'CATG_T
Cuts at: 4876 4876
Size: 5977
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5977
PmeI GTTT'AAAC
Cuts at: 3281 3281
Size: 5977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4046 4078 5559 5591 4046
Size: 32 1481 32 4432
Fragments arranged by size:
4432 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3077 3077
Size: 5977
PshAI GACnn'nnGTC
Cuts at: 1613 2855 1613
Size: 1242 4735
PsiI TTA'TAA
Cuts at: 3547 3547
Size: 5977
PspOMI G'GGCC_C
Cuts at: 2308 2308
Size: 5977
PstI C_TGCA'G
Cuts at: 830 2346 3329 830
Size: 1516 983 3478
Fragments arranged by size:
3478 1516 983
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5977
PvuII CAG'CTG
Cuts at: 2188 2278 2997 3156 2188
Size: 90 719 159 5009
Fragments arranged by size:
5009 719 159 90
SacII CC_GC'GG
Cuts at: 3047 3047
Size: 5977
SalI G'TCGA_C
Cuts at: 1936 3319 1936
Size: 1383 4594
SanDI GG'GwC_CC
Cuts at: 3077 3077
Size: 5977
SapI GCTCTTCn'nnn_
Cuts at: 2264 2264
Size: 5977
SbfI CC_TGCA'GG
Cuts at: 2346 3329 2346
Size: 983 4994
ScaI AGT'ACT
Cuts at: 4225 5937 4225
Size: 1712 4265
SfcI C'TryA_G
Cuts at: 826 1045 2342 2659 3325 4460 5141 5332 826
Size: 219 1297 317 666 1135 681 191 1471
Fragments arranged by size:
1471 1297 1135 681 666 317 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2190 2190
Size: 5977
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
SgrAI Cr'CCGG_yG
Cuts at: 1700 3096 1700
Size: 1396 4581
SmaI CCC'GGG
Cuts at: 1872 3304 1872
Size: 1432 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2505 3121 4081 4982 5244 820
Size: 197 926 562 616 960 901 262 1553
Fragments arranged by size:
1553 960 926 901 616 562 262 197
SnaBI TAC'GTA
Cuts at: 493 3768 493
Size: 3275 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5977
SphI G_CATG'C
Cuts at: 1132 2212 3335 1132
Size: 1080 1123 3774
Fragments arranged by size:
3774 1123 1080
SspI AAT'ATT
Cuts at: 5 52 3901 5
Size: 47 3849 2081
Fragments arranged by size:
3849 2081 47
StuI AGG'CCT
Cuts at: 1517 2711 3211 1517
Size: 1194 500 4283
Fragments arranged by size:
4283 1194 500
StyI C'CwwG_G
Cuts at: 513 1065 2004 2106 2582 3224 3416 513
Size: 552 939 102 476 642 192 3074
Fragments arranged by size:
3074 939 642 552 476 192 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4004 4021 4174 4359 4804 325
Size: 1255 2424 17 153 185 445 1498
Fragments arranged by size:
2424 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4223 5935 96
Size: 274 80 33 51 1443 2246 1712 138
Fragments arranged by size:
2246 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5937 5969 5937
Size: 32 5945
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5977
XbaI T'CTAG_A
Cuts at: 3313 3313
Size: 5977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2280 2280
Size: 5977
XhoI C'TCGA_G
Cuts at: 1943 3121 1943
Size: 1178 4799
XmaI C'CCGG_G
Cuts at: 1870 3302 1870
Size: 1432 4545
XmnI GAAnn'nnTTC
Cuts at: 4106 4106
Size: 5977
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3785 276
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacI SexAI SfoI SrfI SwaI