Restriction map for
pFN21AE0726
(Circular) (Six-base) MAPSORT of: pFN21AE0726.seq Check: 1218 from: 1 to: 5956
pFN21AE0726 5956 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2338 2338
Size: 5956
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3766 278
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2813 3299 1937
Size: 876 486 4594
Fragments arranged by size:
4594 876 486
Acc65I G'GTAC_C
Cuts at: 3277 3277
Size: 5956
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2232 4019 5403 5671 1632
Size: 600 1787 1384 268 1917
Fragments arranged by size:
1917 1787 1384 600 268
AflII C'TTAA_G
Cuts at: 820 1017 3224 820
Size: 197 2207 3552
Fragments arranged by size:
3552 2207 197
AflIII A'CryG_T
Cuts at: 1203 2459 3179 4855 5832 1203
Size: 1256 720 1676 977 1327
Fragments arranged by size:
1676 1327 1256 977 720
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 2610 4685 2610
Size: 2075 3881
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2435 1184
Size: 1251 4705
AlwNI CAG_nnn'CTG
Cuts at: 3699 5271 3699
Size: 1572 4384
ApaLI G'TGCA_C
Cuts at: 4013 5169 4013
Size: 1156 4800
ApoI r'AATT_y
Cuts at: 1493 2116 2872 2976 3265 3491 3643 5752 1493
Size: 623 756 104 289 226 152 2109 1697
Fragments arranged by size:
2109 1697 756 623 289 226 152 104
AseI AT'TA_AT
Cuts at: 160 1032 4510 160
Size: 872 3478 1606
Fragments arranged by size:
3478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 3281 1870
Size: 73 1338 4545
Fragments arranged by size:
4545 1338 73
BamHI G'GATC_C
Cuts at: 2705 3286 3653 2705
Size: 581 367 5008
Fragments arranged by size:
5008 581 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2664 3277 4732 618
Size: 325 217 627 877 613 1455 1842
Fragments arranged by size:
1842 1455 877 627 613 325 217
BanII G_rGCy'C
Cuts at: 1391 3275 1391
Size: 1884 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2274 2535 2706 928
Size: 971 375 261 171 4178
Fragments arranged by size:
4178 971 375 261 171
BbvCI CC'TCA_GC
Cuts at: 2178 2178
Size: 5956
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4147 4181 1903
Size: 34 2210 34 3678
Fragments arranged by size:
3678 2210 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2462 3848 5058 2462
Size: 1386 1210 3360
Fragments arranged by size:
3360 1386 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4567 136
Size: 107 122 71 631 605 2895 1525
Fragments arranged by size:
2895 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5951 5951
Size: 5956
BlpI GC'TnA_GC
Cuts at: 3373 3373
Size: 5956
Bme1580I G_kGCm'C
Cuts at: 1790 2719 4017 5173 1790
Size: 929 1298 1156 2573
Fragments arranged by size:
2573 1298 1156 929
BmgBI CAC'GTC
Cuts at: 1318 2035 2104 1318
Size: 717 69 5170
Fragments arranged by size:
5170 717 69
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2856 3687 4645
Size: 416 53 83 399 24 1439 831 958
Cuts at: 4645 442
Size: 1753
Fragments arranged by size:
1753 1439 958 831 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2246 2788 2246
Size: 542 5414
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2293 2758 2922 3006 3174 4616 1239
Size: 1054 465 164 84 168 1442 2579
Fragments arranged by size:
2579 1442 1054 465 168 164 84
Bpu10I CC'TnA_GC
Cuts at: 2178 2178
Size: 5956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2452 4081 4946 5244 2452
Size: 1629 865 298 3164
Fragments arranged by size:
3164 1629 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4619 882
Size: 617 3120 2219
Fragments arranged by size:
3120 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2462 2636 3747 493
Size: 711 1258 174 1111 2702
Fragments arranged by size:
2702 1258 1111 711 174
BsaBI GATnn'nnATC
Cuts at: 5950 5950
Size: 5956
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3763 4145 4780 275
Size: 53 83 186 936 2230 382 635 1451
Fragments arranged by size:
2230 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2876 4389 4787 5061 5208 5619 2876
Size: 1513 398 274 147 411 3213
Fragments arranged by size:
3213 1513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5731
Fragments arranged by size:
5731 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 1186
Size: 886 5070
BseYI C'CCAG_C
Cuts at: 2077 2247 5159 5655 2077
Size: 170 2912 496 2378
Fragments arranged by size:
2912 2378 496 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3092 1163
Size: 573 1356 4027
Fragments arranged by size:
4027 1356 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3406 4167 5195 1540
Size: 275 186 1405 761 1028 2301
Fragments arranged by size:
2301 1405 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4017 4102 5173 4017
Size: 85 1071 4800
Fragments arranged by size:
4800 1071 85
BsmI GAATG_Cn'
Cuts at: 2132 3467 3560 2132
Size: 1335 93 4528
Fragments arranged by size:
4528 1335 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2094 2094
Size: 5956
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2719 3275 4017 4102 5173 1391
Size: 399 929 556 742 85 1071 2174
Fragments arranged by size:
2174 1071 929 742 556 399 85
BspEI T'CCGG_A
Cuts at: 5619 5619
Size: 5956
BspHI T'CATG_A
Cuts at: 2380 3845 5575 2380
Size: 1465 1730 2761
Fragments arranged by size:
2761 1730 1465
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2338 2417 3311 844
Size: 1494 79 894 3489
Fragments arranged by size:
3489 1494 894 79
BsrBI CCG'CTC
Cuts at: 1394 1823 3843 1394
Size: 429 2020 3507
Fragments arranged by size:
3507 2020 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2893 4451 4625 65
Size: 1183 1645 1558 174 1396
Fragments arranged by size:
1645 1558 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4600 4787 1700
Size: 110 2790 187 2869
Fragments arranged by size:
2869 2790 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5956
BssSI C'ACGA_G
Cuts at: 2233 4016 5028 2233
Size: 1783 1012 3161
Fragments arranged by size:
3161 1783 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2858 3070 794
Size: 2064 212 3680
Fragments arranged by size:
3680 2064 212
BstBI TT'CG_AA
Cuts at: 4760 4760
Size: 5956
BstEII G'GTnAC_C
Cuts at: 1184 2020 1184
Size: 836 5120
BstYI r'GATC_y
Cuts at: 1972 2705 3286 3653 4038 4055 5507 5593 5605
Size: 733 581 367 385 17 1452 86 12
Cuts at: 5605 5951 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 733 581 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2814 2814
Size: 5956
Bsu36I CC'TnA_GG
Cuts at: 2711 2711
Size: 5956
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5404
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5217
Fragments arranged by size:
5217 683 56
BtsI GCAGTG_nn'
Cuts at: 3472 4266 4286 3472
Size: 794 20 5142
Fragments arranged by size:
5142 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5921
DraI TTT'AAA
Cuts at: 3260 3260
Size: 5956
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2537 4963 809
Size: 507 1221 2426 1802
Fragments arranged by size:
2426 1802 1221 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3403 4292 5921
Size: 54 1425 50 275 18 1573 889 1629
Cuts at: 5921 8
Size: 43
Fragments arranged by size:
1629 1573 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3403 1537
Size: 275 1591 4090
Fragments arranged by size:
4090 1591 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3886 1353
Size: 2533 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4543 4917 5063 387
Size: 1064 138 2954 374 146 1280
Fragments arranged by size:
2954 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 2293 2758 2922 3006 3174 4019
Size: 393 600 61 465 164 84 168 845
Cuts at: 4019 4616 5403 5671 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 845 787 600 597 465 393 268
168 164 84 61
EcoRI G'AATT_C
Cuts at: 3265 3643 3265
Size: 378 5578
FspI TGC'GCA
Cuts at: 4462 4462
Size: 5956
HaeII r_GCGC'y
Cuts at: 1678 5103 1678
Size: 3425 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2436 2468 2576 2596 2608
Size: 32 135 32 864 32 108 20 12
Cuts at: 2608 2628 4597 4629 4671 4703 1373
Size: 20 1969 32 42 32 2626
Fragments arranged by size:
2626 1969 864 135 108 42 32 32
32 32 32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3300 3546 669
Size: 988 281 1362 246 3079
Fragments arranged by size:
3079 1362 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2688 748
Size: 1940 4016
HpaI GTT'AAC
Cuts at: 3546 3546
Size: 5956
KpnI G_GTAC'C
Cuts at: 3281 3281
Size: 5956
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5070 5254 1305
Size: 3765 184 2007
Fragments arranged by size:
3765 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4134
Fragments arranged by size:
4134 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2634 2801 3237 3914 4273 4432 518
Size: 648 1468 167 436 677 359 159 2042
Fragments arranged by size:
2042 1468 677 648 436 359 167 159
MspA1I CmG'CkG
Cuts at: 1606 2671 3372 4049 5197 5442 1606
Size: 1065 701 677 1148 245 2120
Fragments arranged by size:
2120 1148 1065 701 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5404
NdeI CA'TA_TG
Cuts at: 387 2970 387
Size: 2583 3373
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 3403 3403
Size: 5956
NspI r_CATG'y
Cuts at: 1132 1229 3068 3183 3314 4859 1132
Size: 97 1839 115 131 1545 2229
Fragments arranged by size:
2229 1839 1545 131 115 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5956
PciI A'CATG_T
Cuts at: 3179 4855 3179
Size: 1676 4280
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5956
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PmlI CAC'GTG
Cuts at: 2462 2462
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2227 2259 4025 4057 5538 5570 2227
Size: 32 1766 32 1481 32 2613
Fragments arranged by size:
2613 1766 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5956
PsiI TTA'TAA
Cuts at: 3526 3526
Size: 5956
PstI C_TGCA'G
Cuts at: 830 2335 2414 3308 830
Size: 1505 79 894 3478
Fragments arranged by size:
3478 1505 894 79
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
SalI G'TCGA_C
Cuts at: 1936 3298 1936
Size: 1362 4594
SbfI CC_TGCA'GG
Cuts at: 2414 3308 2414
Size: 894 5062
ScaI AGT'ACT
Cuts at: 4204 5916 4204
Size: 1712 4244
SfcI C'TryA_G
Cuts at: 826 1045 2095 2331 2410 3304 4439 5120 5311
Size: 219 1050 236 79 894 1135 681 191
Cuts at: 5311 826
Size: 1471
Fragments arranged by size:
1471 1135 1050 894 681 236 219 191
79
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 3283 1872
Size: 1411 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2467 3224 4060 4961 5223 820
Size: 197 926 524 757 836 901 262 1553
Fragments arranged by size:
1553 926 901 836 757 524 262 197
SnaBI TAC'GTA
Cuts at: 493 3747 493
Size: 3254 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 3314 1132
Size: 2182 3774
SspI AAT'ATT
Cuts at: 5 52 3880 5
Size: 47 3828 2081
Fragments arranged by size:
3828 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5956
StyI C'CwwG_G
Cuts at: 513 1065 3395 513
Size: 552 2330 3074
Fragments arranged by size:
3074 2330 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3983 4000 4153 4338 4783 325
Size: 1255 2403 17 153 185 445 1498
Fragments arranged by size:
2403 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4202 5914 96
Size: 274 80 33 51 1443 2225 1712 138
Fragments arranged by size:
2225 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5916 5948 5916
Size: 32 5924
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5956
XbaI T'CTAG_A
Cuts at: 3292 3292
Size: 5956
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5956
XmaI C'CCGG_G
Cuts at: 1870 3281 1870
Size: 1411 4545
XmnI GAAnn'nnTTC
Cuts at: 4085 4085
Size: 5956
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3764 276
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstXI DraIII EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI