Restriction map for
pFN21AE0724
(Circular) (Six-base) MAPSORT of: pFN21AE0724.seq Check: 3758 from: 1 to: 5950
pFN21AE0724 5950 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3760 278
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2319 3293 1937
Size: 382 974 4594
Fragments arranged by size:
4594 974 382
Acc65I G'GTAC_C
Cuts at: 2793 3271 2793
Size: 478 5472
AclI AA'CG_TT
Cuts at: 1563 4077 4450 1563
Size: 2514 373 3063
Fragments arranged by size:
3063 2514 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4013 5397 5665 1632
Size: 2381 1384 268 1917
Fragments arranged by size:
2381 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5753
AflIII A'CryG_T
Cuts at: 1203 2724 4849 5826 1203
Size: 1521 2125 977 1327
Fragments arranged by size:
2125 1521 1327 977
AgeI A'CCGG_T
Cuts at: 2274 4781 2274
Size: 2507 3443
AhdI GACnn_n'nnGTC
Cuts at: 4679 4679
Size: 5950
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5950
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2261 2293 2375 2407 2261
Size: 32 82 32 5804
Fragments arranged by size:
5804 82 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3167 3693 5265 3167
Size: 526 1572 3852
Fragments arranged by size:
3852 1572 526
ApaI G_GGCC'C
Cuts at: 2135 2254 2376 2135
Size: 119 122 5709
Fragments arranged by size:
5709 122 119
ApaLI G'TGCA_C
Cuts at: 2471 4007 5163 2471
Size: 1536 1156 3258
Fragments arranged by size:
3258 1536 1156
ApoI r'AATT_y
Cuts at: 1493 3259 3485 3637 5746 1493
Size: 1766 226 152 2109 1697
Fragments arranged by size:
2109 1766 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4504 160
Size: 872 3472 1606
Fragments arranged by size:
3472 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
AvaI C'yCGr_G
Cuts at: 1870 1943 2101 2113 2247 2507 2530 2597 3275
Size: 73 158 12 134 260 23 67 678
Cuts at: 3275 1870
Size: 4545
Fragments arranged by size:
4545 678 260 158 134 73 67 23
12
AvrII C'CTAG_G
Cuts at: 2188 2188
Size: 5950
BamHI G'GATC_C
Cuts at: 3280 3647 3280
Size: 367 5583
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2793 2889 3020 3271 4726
Size: 325 217 627 1006 96 131 251 1455
Cuts at: 4726 618
Size: 1842
Fragments arranged by size:
1842 1455 1006 627 325 251 217 131
96
BanII G_rGCy'C
Cuts at: 1391 2102 2135 2184 2254 2376 2769 3269 1391
Size: 711 33 49 70 122 393 500 4072
Fragments arranged by size:
4072 711 500 393 122 70 49 33
BbeI G_GCGC'C
Cuts at: 2893 2893
Size: 5950
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4979
BbvCI CC'TCA_GC
Cuts at: 3051 3051
Size: 5950
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2140 2174 2947 2981 4141 4175 1903
Size: 34 203 34 773 34 1160 34 3678
Fragments arranged by size:
3678 1160 773 203 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3842 5052 3842
Size: 1210 4740
BclI T'GATC_A
Cuts at: 1548 2451 1548
Size: 903 5047
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2643 4561 136
Size: 107 122 71 631 605 971 1918 1525
Fragments arranged by size:
1918 1525 971 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5945 5945
Size: 5950
BlpI GC'TnA_GC
Cuts at: 2291 3367 2291
Size: 1076 4874
Bme1580I G_kGCm'C
Cuts at: 1790 2135 2254 2376 2475 2648 4011 5167 1790
Size: 345 119 122 99 173 1363 1156 2573
Fragments arranged by size:
2573 1363 1156 345 173 122 119 99
BmgBI CAC'GTC
Cuts at: 1318 2500 1318
Size: 1182 4768
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2998 3681 4639
Size: 416 53 83 399 24 1581 683 958
Cuts at: 4639 442
Size: 1753
Fragments arranged by size:
1753 1581 958 683 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2288 2381 2402 2729 4610 1239
Size: 1049 93 21 327 1881 2579
Fragments arranged by size:
2579 1881 1049 327 93 21
Bpu10I CC'TnA_GC
Cuts at: 3051 3051
Size: 5950
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2405 2753 2768 4075 4940 5238 2405
Size: 348 15 1307 865 298 3117
Fragments arranged by size:
3117 1307 865 348 298 15
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2594 4613 882
Size: 617 1095 2019 2219
Fragments arranged by size:
2219 2019 1095 617
BsaAI yAC'GTr
Cuts at: 493 1204 2218 2871 3741 493
Size: 711 1014 653 870 2702
Fragments arranged by size:
2702 1014 870 711 653
BsaBI GATnn'nnATC
Cuts at: 2441 5944 2441
Size: 3503 2447
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2307 2890 3757 4139
Size: 53 83 186 936 774 583 867 382
Cuts at: 4139 4774 275
Size: 635 1451
Fragments arranged by size:
1451 936 867 774 635 583 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2094 2274 2406 4383 4781 5055 5202 5613 2094
Size: 180 132 1977 398 274 147 411 2431
Fragments arranged by size:
2431 1977 411 398 274 180 147 132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2261 2291 2375 2405 3026
Size: 30 165 30 858 30 84 30 621
Cuts at: 3026 3056 1178
Size: 30 4072
Fragments arranged by size:
4072 858 621 165 84 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2873 3232 1186
Size: 871 816 359 3904
Fragments arranged by size:
3904 871 816 359
BseYI C'CCAG_C
Cuts at: 2368 2375 2716 3045 3069 5153 5649 2368
Size: 7 341 329 24 2084 496 2669
Fragments arranged by size:
2669 2084 496 341 329 24 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2246 1163
Size: 573 510 4867
Fragments arranged by size:
4867 573 510
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2407 2956 3138 3400 4161 5189
Size: 275 186 406 549 182 262 761 1028
Cuts at: 5189 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 549 406 275 262 186
182
BsiHKAI G_wGCw'C
Cuts at: 2102 2184 2475 2631 2841 4011 4096 5167 2102
Size: 82 291 156 210 1170 85 1071 2885
Fragments arranged by size:
2885 1170 1071 291 210 156 85 82
BsmI GAATG_Cn'
Cuts at: 3124 3461 3554 3124
Size: 337 93 5520
Fragments arranged by size:
5520 337 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2030 2030
Size: 5950
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2102 2135 2184 2254 2376 2475 2631
Size: 399 312 33 49 70 122 99 156
Cuts at: 2631 2648 2769 2841 3269 4011 4096 5167 1391
Size: 17 121 72 428 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 428 399 312 156 122
121 99 85 72 70 49 33 17
BspEI T'CCGG_A
Cuts at: 2094 5613 2094
Size: 3519 2431
BspHI T'CATG_A
Cuts at: 3839 5569 3839
Size: 1730 4220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2883 2934 3045 3305 844
Size: 2039 51 111 260 3489
Fragments arranged by size:
3489 2039 260 111 51
BsrBI CCG'CTC
Cuts at: 1394 1823 2791 3837 1394
Size: 429 968 1046 3507
Fragments arranged by size:
3507 1046 968 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4445 4619 65
Size: 1183 3197 174 1396
Fragments arranged by size:
3197 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2066 2274 3137 4594 4781 1700
Size: 110 256 208 863 1457 187 2869
Fragments arranged by size:
2869 1457 863 256 208 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5950
BssHII G'CGCG_C
Cuts at: 1925 2651 1925
Size: 726 5224
BssSI C'ACGA_G
Cuts at: 4010 5022 4010
Size: 1012 4938
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5950
BstBI TT'CG_AA
Cuts at: 4754 4754
Size: 5950
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5950
BstXI CCAn_nnnn'nTGG
Cuts at: 2359 2359
Size: 5950
BstYI r'GATC_y
Cuts at: 1972 3280 3647 4032 4049 5501 5587 5599 5945
Size: 1308 367 385 17 1452 86 12 346
Cuts at: 5945 1972
Size: 1977
Fragments arranged by size:
1977 1452 1308 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2313 2313
Size: 5950
BtgI C'CryG_G
Cuts at: 513 1065 2004 2086 2352 3132 513
Size: 552 939 82 266 780 3331
Fragments arranged by size:
3331 939 780 552 266 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5211
Fragments arranged by size:
5211 683 56
BtsI GCAGTG_nn'
Cuts at: 2965 3466 4260 4280 2965
Size: 501 794 20 4635
Fragments arranged by size:
4635 794 501 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5950
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5915
DraI TTT'AAA
Cuts at: 3254 3254
Size: 5950
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4957 809
Size: 507 3641 1802
Fragments arranged by size:
3641 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2577 2865 3135
Size: 54 1425 50 275 18 747 288 270
Cuts at: 3135 3139 3183 3397 4286 5915 8
Size: 4 44 214 889 1629 43
Fragments arranged by size:
1629 1425 889 747 288 275 270 214
54 50 44 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 3135 3397 1537
Size: 275 1323 262 4090
Fragments arranged by size:
4090 1323 275 262
EarI CTCTTCn'nnn_
Cuts at: 1353 2942 2954 3880 1353
Size: 1589 12 926 3423
Fragments arranged by size:
3423 1589 926 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2174 4537 4911 5057 387
Size: 1064 138 585 2363 374 146 1280
Fragments arranged by size:
2363 1280 1064 585 374 146 138
EcoICRI GAG'CTC
Cuts at: 2100 2182 2100
Size: 82 5868
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2288 2381 2402 2729 4013 4610 5397
Size: 393 656 93 21 327 1284 597 787
Cuts at: 5397 5665 1239
Size: 268 1524
Fragments arranged by size:
1524 1284 787 656 597 393 327 268
93 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2681 2681
Size: 5950
EcoO109I rG'GnC_Cy
Cuts at: 2119 2132 2251 2372 2386 2119
Size: 13 119 121 14 5683
Fragments arranged by size:
5683 121 119 14 13
EcoRI G'AATT_C
Cuts at: 3259 3637 3259
Size: 378 5572
FseI GG_CCGG'CC
Cuts at: 3141 3141
Size: 5950
FspI TGC'GCA
Cuts at: 4456 4456
Size: 5950
HaeII r_GCGC'y
Cuts at: 1678 2298 2717 2893 3167 5097 1678
Size: 620 419 176 274 1930 2531
Fragments arranged by size:
2531 1930 620 419 274 176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3026 3058 4591 4623 4665
Size: 32 135 32 1454 32 1533 32 42
Cuts at: 4665 4697 1373
Size: 32 2626
Fragments arranged by size:
2626 1533 1454 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3294 3540 669
Size: 988 281 1356 246 3079
Fragments arranged by size:
3079 1356 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5950
HpaI GTT'AAC
Cuts at: 3540 3540
Size: 5950
KasI G'GCGC_C
Cuts at: 2889 2889
Size: 5950
KpnI G_GTAC'C
Cuts at: 2797 3275 2797
Size: 478 5472
MfeI C'AATT_G
Cuts at: 3549 3549
Size: 5950
MluI A'CGCG_T
Cuts at: 5826 5826
Size: 5950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3171 5064 5248 1305
Size: 1866 1893 184 2007
Fragments arranged by size:
2007 1893 1866 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2579 2867 10
Size: 54 1425 343 747 288 3093
Fragments arranged by size:
3093 1425 747 343 288 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2459 2729 2834 3908 4267 4426 518
Size: 648 1293 270 105 1074 359 159 2042
Fragments arranged by size:
2042 1293 1074 648 359 270 159 105
MspA1I CmG'CkG
Cuts at: 1606 2260 2449 3366 4043 5191 5436 1606
Size: 654 189 917 677 1148 245 2120
Fragments arranged by size:
2120 1148 917 677 654 245 189
NaeI GCC'GGC
Cuts at: 2068 3139 2068
Size: 1071 4879
NarI GG'CG_CC
Cuts at: 2890 2890
Size: 5950
NcoI C'CATG_G
Cuts at: 513 1065 2004 2352 513
Size: 552 939 348 4111
Fragments arranged by size:
4111 939 552 348
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5950
NgoMIV G'CCGG_C
Cuts at: 2066 3137 2066
Size: 1071 4879
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5950
NotI GC'GGCC_GC
Cuts at: 3397 3397
Size: 5950
NspI r_CATG'y
Cuts at: 1132 1229 2552 2728 3308 4853 1132
Size: 97 1323 176 580 1545 2229
Fragments arranged by size:
2229 1545 1323 580 176 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5950
PciI A'CATG_T
Cuts at: 2724 4849 2724
Size: 2125 3825
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5950
PfoI T'CCnGG_A
Cuts at: 3057 3057
Size: 5950
PmeI GTTT'AAAC
Cuts at: 3254 3254
Size: 5950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2261 2293 2375 2407 4019 4051 5532 5564 2261
Size: 32 82 32 1612 32 1481 32 2647
Fragments arranged by size:
2647 1612 1481 82 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2119 2386 2119
Size: 267 5683
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5950
PsiI TTA'TAA
Cuts at: 3520 3520
Size: 5950
PspOMI G'GGCC_C
Cuts at: 2131 2250 2372 2131
Size: 119 122 5709
Fragments arranged by size:
5709 122 119
PstI C_TGCA'G
Cuts at: 830 3172 3240 3302 830
Size: 2342 68 62 3478
Fragments arranged by size:
3478 2342 68 62
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5950
PvuII CAG'CTG
Cuts at: 2260 2449 2260
Size: 189 5761
RsrII CG'GwC_CG
Cuts at: 2330 2330
Size: 5950
SacI G_AGCT'C
Cuts at: 2102 2184 2102
Size: 82 5868
SalI G'TCGA_C
Cuts at: 1936 3292 1936
Size: 1356 4594
SapI GCTCTTCn'nnn_
Cuts at: 2942 2942
Size: 5950
SbfI CC_TGCA'GG
Cuts at: 3302 3302
Size: 5950
ScaI AGT'ACT
Cuts at: 4198 5910 4198
Size: 1712 4238
SfcI C'TryA_G
Cuts at: 826 1045 3168 3236 3298 4433 5114 5305 826
Size: 219 2123 68 62 1135 681 191 1471
Fragments arranged by size:
2123 1471 1135 681 219 191 68 62
SfoI GGC'GCC
Cuts at: 2891 2891
Size: 5950
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5950
SmaI CCC'GGG
Cuts at: 1872 2249 2532 3277 1872
Size: 377 283 745 4545
Fragments arranged by size:
4545 745 377 283
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2420 2768 2783 4054 4955 5217
Size: 197 926 477 348 15 1271 901 262
Cuts at: 5217 820
Size: 1553
Fragments arranged by size:
1553 1271 926 901 477 348 262 197
15
SnaBI TAC'GTA
Cuts at: 493 3741 493
Size: 3248 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5950
SphI G_CATG'C
Cuts at: 1132 2552 3308 1132
Size: 1420 756 3774
Fragments arranged by size:
3774 1420 756
SspI AAT'ATT
Cuts at: 5 52 3874 5
Size: 47 3822 2081
Fragments arranged by size:
3822 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5950
StyI C'CwwG_G
Cuts at: 513 1065 2004 2061 2188 2352 2580 3389 513
Size: 552 939 57 127 164 228 809 3074
Fragments arranged by size:
3074 939 809 552 228 164 127 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2318 3977 3994 4147 4332 4777 325
Size: 1255 738 1659 17 153 185 445 1498
Fragments arranged by size:
1659 1498 1255 738 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2343 2586 2733
Size: 274 80 33 51 1443 366 243 147
Cuts at: 2733 4196 5908 96
Size: 1463 1712 138
Fragments arranged by size:
1712 1463 1443 366 274 243 147 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5910 5942 5910
Size: 32 5918
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5950
XbaI T'CTAG_A
Cuts at: 3286 3286
Size: 5950
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5950
XmaI C'CCGG_G
Cuts at: 1870 2247 2530 3275 1870
Size: 377 283 745 4545
Fragments arranged by size:
4545 745 377 283
XmnI GAAnn'nnTTC
Cuts at: 2936 4079 2936
Size: 1143 4807
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3758 276
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BfrBI BplI BsiWI BstZ17I
DraIII EcoRV FalI FspAI NruI NsiI PacI PmlI
PspXI PsrI SacII SanDI SexAI SfiI SrfI SwaI
XcmI