Restriction map for
pFN21AE0723
(Circular) (Six-base) MAPSORT of: pFN21AE0723.seq Check: 9042 from: 1 to: 5623
pFN21AE0723 5623 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3433 278
Size: 53 83 186 936 1897 2468
Fragments arranged by size:
2468 1897 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2966 1937
Size: 1029 4594
Acc65I G'GTAC_C
Cuts at: 2944 2944
Size: 5623
AclI AA'CG_TT
Cuts at: 1563 3750 4123 1563
Size: 2187 373 3063
Fragments arranged by size:
3063 2187 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2003 3686 5070 5338 1632
Size: 371 1683 1384 268 1917
Fragments arranged by size:
1917 1683 1384 371 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5426
AflIII A'CryG_T
Cuts at: 1203 4522 5499 1203
Size: 3319 977 1327
Fragments arranged by size:
3319 1327 977
AgeI A'CCGG_T
Cuts at: 4454 4454
Size: 5623
AhdI GACnn_n'nnGTC
Cuts at: 2053 2450 4352 2053
Size: 397 1902 3324
Fragments arranged by size:
3324 1902 397
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5623
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2579 2655 1184
Size: 1395 76 4152
Fragments arranged by size:
4152 1395 76
AlwNI CAG_nnn'CTG
Cuts at: 2257 2321 3366 4938 2257
Size: 64 1045 1572 2942
Fragments arranged by size:
2942 1572 1045 64
ApaLI G'TGCA_C
Cuts at: 2162 3680 4836 2162
Size: 1518 1156 2949
Fragments arranged by size:
2949 1518 1156
ApoI r'AATT_y
Cuts at: 1493 2932 3158 3310 5419 1493
Size: 1439 226 152 2109 1697
Fragments arranged by size:
2109 1697 1439 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4177 160
Size: 872 3145 1606
Fragments arranged by size:
3145 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5623
AvaI C'yCGr_G
Cuts at: 1870 1943 2067 2208 2448 2829 2948 1870
Size: 73 124 141 240 381 119 4545
Fragments arranged by size:
4545 381 240 141 124 119 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2472 2505 2472
Size: 33 5590
BamHI G'GATC_C
Cuts at: 2953 3320 2953
Size: 367 5256
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2690 2944 4399 618
Size: 325 217 627 903 254 1455 1842
Fragments arranged by size:
1842 1455 903 627 325 254 217
BanII G_rGCy'C
Cuts at: 1391 2856 2942 1391
Size: 1465 86 4072
Fragments arranged by size:
4072 1465 86
BbeI G_GCGC'C
Cuts at: 2694 2694
Size: 5623
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2065 928
Size: 971 166 4486
Fragments arranged by size:
4486 971 166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3814 3848 1903
Size: 34 1877 34 3678
Fragments arranged by size:
3678 1877 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3515 4725 3515
Size: 1210 4413
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5623
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4234 136
Size: 107 122 71 631 605 2562 1525
Fragments arranged by size:
2562 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5618 5618
Size: 5623
BlpI GC'TnA_GC
Cuts at: 2533 2618 3040 2533
Size: 85 422 5116
Fragments arranged by size:
5116 422 85
Bme1580I G_kGCm'C
Cuts at: 1790 2166 2424 3684 4840 1790
Size: 376 258 1260 1156 2573
Fragments arranged by size:
2573 1260 1156 376 258
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5623
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3354 4312 442
Size: 416 53 83 399 24 1937 958 1753
Fragments arranged by size:
1937 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5623
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2295 4283 1239
Size: 1056 1988 2579
Fragments arranged by size:
2579 1988 1056
Bpu10I CC'TnA_GC
Cuts at: 2283 2283
Size: 5623
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3748 4613 4911 3748
Size: 865 298 4460
Fragments arranged by size:
4460 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2114 4286 882
Size: 617 615 2172 2219
Fragments arranged by size:
2219 2172 617 615
BsaAI yAC'GTr
Cuts at: 493 1204 3414 493
Size: 711 2210 2702
Fragments arranged by size:
2702 2210 711
BsaBI GATnn'nnATC
Cuts at: 5617 5617
Size: 5623
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2691 3430 3812 4447
Size: 53 83 186 936 1158 739 382 635
Cuts at: 4447 275
Size: 1451
Fragments arranged by size:
1451 1158 936 739 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4056 4454 4728 4875 5286 4056
Size: 398 274 147 411 4393
Fragments arranged by size:
4393 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5398
Fragments arranged by size:
5398 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5623
BseYI C'CCAG_C
Cuts at: 2221 4826 5322 2221
Size: 2605 496 2522
Fragments arranged by size:
2605 2522 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5050
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3073 3834 4862 1540
Size: 275 186 1072 761 1028 2301
Fragments arranged by size:
2301 1072 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2166 3684 3769 4840 2166
Size: 1518 85 1071 2949
Fragments arranged by size:
2949 1518 1071 85
BsmI GAATG_Cn'
Cuts at: 3134 3227 3134
Size: 93 5530
BsmBI CGTCTCn'nnnn_
Cuts at: 2826 2826
Size: 5623
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2166 2424 2856 2942 3684 3769 4840
Size: 399 376 258 432 86 742 85 1071
Cuts at: 4840 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 432 399 376 258 86
85
BspEI T'CCGG_A
Cuts at: 5286 5286
Size: 5623
BspHI T'CATG_A
Cuts at: 3512 5242 3512
Size: 1730 3893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2911 2978 844
Size: 2067 67 3489
Fragments arranged by size:
3489 2067 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2511 3510 1394
Size: 429 688 999 3507
Fragments arranged by size:
3507 999 688 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2035 4118 4292 65
Size: 1183 787 2083 174 1396
Fragments arranged by size:
2083 1396 1183 787 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2150 2693 2763 4267 4454 1700
Size: 110 340 543 70 1504 187 2869
Fragments arranged by size:
2869 1504 543 340 187 110 70
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5623
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5623
BssSI C'ACGA_G
Cuts at: 3683 4695 3683
Size: 1012 4611
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2321 794
Size: 1527 4096
BstBI TT'CG_AA
Cuts at: 4427 4427
Size: 5623
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5623
BstYI r'GATC_y
Cuts at: 1972 2953 3320 3705 3722 5174 5260 5272 5618
Size: 981 367 385 17 1452 86 12 346
Cuts at: 5618 1972
Size: 1977
Fragments arranged by size:
1977 1452 981 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5071
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2856 487
Size: 683 56 1630 3254
Fragments arranged by size:
3254 1630 683 56
BtsI GCAGTG_nn'
Cuts at: 2563 2740 3139 3933 3953 2563
Size: 177 399 794 20 4233
Fragments arranged by size:
4233 794 399 177 20
ClaI AT'CG_AT
Cuts at: 1554 2607 1554
Size: 1053 4570
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5588
DraI TTT'AAA
Cuts at: 2927 2927
Size: 5623
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4630 809
Size: 507 3314 1802
Fragments arranged by size:
3314 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2140 2148 2244
Size: 54 1425 50 275 18 310 8 96
Cuts at: 2244 2765 3070 3959 5588 8
Size: 521 305 889 1629 43
Fragments arranged by size:
1629 1425 889 521 310 305 275 96
54 50 43 18 8
EagI C'GGCC_G
Cuts at: 1537 1812 3070 1537
Size: 275 1258 4090
Fragments arranged by size:
4090 1258 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2390 2868 3553 1353
Size: 1037 478 685 3423
Fragments arranged by size:
3423 1037 685 478
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4210 4584 4730 387
Size: 1064 138 2621 374 146 1280
Fragments arranged by size:
2621 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2003 2295 3686 4283 5070 5338 1239
Size: 393 371 292 1391 597 787 268 1524
Fragments arranged by size:
1524 1391 787 597 393 371 292 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2810 2810
Size: 5623
EcoO109I rG'GnC_Cy
Cuts at: 2913 2913
Size: 5623
EcoRI G'AATT_C
Cuts at: 2932 3310 2932
Size: 378 5245
FspI TGC'GCA
Cuts at: 4129 4129
Size: 5623
HaeII r_GCGC'y
Cuts at: 1678 2203 2694 4770 1678
Size: 525 491 2076 2531
Fragments arranged by size:
2531 2076 525 491
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 2436 2468 4264
Size: 32 135 32 467 32 365 32 1796
Cuts at: 4264 4296 4338 4370 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1796 467 365 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2967 3213 669
Size: 988 281 1029 246 3079
Fragments arranged by size:
3079 1029 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2015 748
Size: 1267 4356
HpaI GTT'AAC
Cuts at: 3213 3213
Size: 5623
KasI G'GCGC_C
Cuts at: 2690 2690
Size: 5623
KpnI G_GTAC'C
Cuts at: 2948 2948
Size: 5623
MfeI C'AATT_G
Cuts at: 3222 3222
Size: 5623
MluI A'CGCG_T
Cuts at: 5499 5499
Size: 5623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4737 4921 1305
Size: 3432 184 2007
Fragments arranged by size:
3432 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3801
Fragments arranged by size:
3801 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2267 3581 3940 4099 518
Size: 648 1101 1314 359 159 2042
Fragments arranged by size:
2042 1314 1101 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2216 2519 2721 3039 3716 4864 5109 1606
Size: 610 303 202 318 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 610 318 303 245 202
NaeI GCC'GGC
Cuts at: 2152 2695 2765 2152
Size: 543 70 5010
Fragments arranged by size:
5010 543 70
NarI GG'CG_CC
Cuts at: 2691 2691
Size: 5623
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5071
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5623
NgoMIV G'CCGG_C
Cuts at: 2150 2693 2763 2150
Size: 543 70 5010
Fragments arranged by size:
5010 543 70
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5623
NotI GC'GGCC_GC
Cuts at: 3070 3070
Size: 5623
NspI r_CATG'y
Cuts at: 1132 1229 2981 4526 1132
Size: 97 1752 1545 2229
Fragments arranged by size:
2229 1752 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2424 2812 1348
Size: 1076 388 4159
Fragments arranged by size:
4159 1076 388
PciI A'CATG_T
Cuts at: 4522 4522
Size: 5623
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5623
PmeI GTTT'AAAC
Cuts at: 2927 2927
Size: 5623
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3692 3724 5205 5237 3692
Size: 32 1481 32 4078
Fragments arranged by size:
4078 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5623
PsiI TTA'TAA
Cuts at: 3193 3193
Size: 5623
PstI C_TGCA'G
Cuts at: 830 2568 2975 830
Size: 1738 407 3478
Fragments arranged by size:
3478 1738 407
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5623
PvuII CAG'CTG
Cuts at: 2216 2519 2216
Size: 303 5320
SalI G'TCGA_C
Cuts at: 1936 2965 1936
Size: 1029 4594
SapI GCTCTTCn'nnn_
Cuts at: 2390 2868 2390
Size: 478 5145
SbfI CC_TGCA'GG
Cuts at: 2975 2975
Size: 5623
ScaI AGT'ACT
Cuts at: 2189 3871 5583 2189
Size: 1682 1712 2229
Fragments arranged by size:
2229 1712 1682
SfcI C'TryA_G
Cuts at: 826 1045 2564 2971 4106 4787 4978 826
Size: 219 1519 407 1135 681 191 1471
Fragments arranged by size:
1519 1471 1135 681 407 219 191
SfoI GGC'GCC
Cuts at: 2692 2692
Size: 5623
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5623
SgrAI Cr'CCGG_yG
Cuts at: 1700 2693 1700
Size: 993 4630
SmaI CCC'GGG
Cuts at: 1872 2210 2450 2950 1872
Size: 338 240 500 4545
Fragments arranged by size:
4545 500 338 240
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2067 3727 4628 4890 820
Size: 197 926 124 1660 901 262 1553
Fragments arranged by size:
1660 1553 926 901 262 197 124
SnaBI TAC'GTA
Cuts at: 493 3414 493
Size: 2921 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5623
SphI G_CATG'C
Cuts at: 1132 2981 1132
Size: 1849 3774
SrfI GCCC'GGGC
Cuts at: 2210 2210
Size: 5623
SspI AAT'ATT
Cuts at: 5 52 3547 5
Size: 47 3495 2081
Fragments arranged by size:
3495 2081 47
StuI AGG'CCT
Cuts at: 1517 2805 1517
Size: 1288 4335
StyI C'CwwG_G
Cuts at: 513 1065 2298 3062 513
Size: 552 1233 764 3074
Fragments arranged by size:
3074 1233 764 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3650 3667 3820 4005 4450 325
Size: 1255 2070 17 153 185 445 1498
Fragments arranged by size:
2070 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2187 3869 5581
Size: 274 80 33 51 1443 210 1682 1712
Cuts at: 5581 96
Size: 138
Fragments arranged by size:
1712 1682 1443 274 210 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5583 5615 5583
Size: 32 5591
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5623
XbaI T'CTAG_A
Cuts at: 2959 2959
Size: 5623
XhoI C'TCGA_G
Cuts at: 1943 2067 1943
Size: 124 5499
XmaI C'CCGG_G
Cuts at: 1870 2208 2448 2948 1870
Size: 338 240 500 4545
Fragments arranged by size:
4545 500 338 240
XmnI GAAnn'nnTTC
Cuts at: 3752 3752
Size: 5623
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3431 276
Size: 53 83 186 936 1897 2468
Fragments arranged by size:
2468 1897 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SwaI XcmI