Restriction map for
pFN21AE0722
(Circular) (Six-base) MAPSORT of: pFN21AE0722.seq Check: 5849 from: 1 to: 5950
pFN21AE0722 5950 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2183 2497 2183
Size: 314 5636
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3760 278
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2571 3033 3293 1937
Size: 634 462 260 4594
Fragments arranged by size:
4594 634 462 260
Acc65I G'GTAC_C
Cuts at: 3271 3271
Size: 5950
AclI AA'CG_TT
Cuts at: 1563 4077 4450 1563
Size: 2514 373 3063
Fragments arranged by size:
3063 2514 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4013 5397 5665 1632
Size: 2381 1384 268 1917
Fragments arranged by size:
2381 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5753
AflIII A'CryG_T
Cuts at: 1203 4849 5826 1203
Size: 3646 977 1327
Fragments arranged by size:
3646 1327 977
AgeI A'CCGG_T
Cuts at: 4781 4781
Size: 5950
AhdI GACnn_n'nnGTC
Cuts at: 4679 4679
Size: 5950
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2601 1184
Size: 1417 4533
AlwNI CAG_nnn'CTG
Cuts at: 2981 3693 5265 2981
Size: 712 1572 3666
Fragments arranged by size:
3666 1572 712
ApaI G_GGCC'C
Cuts at: 2143 2143
Size: 5950
ApaLI G'TGCA_C
Cuts at: 2699 4007 5163 2699
Size: 1308 1156 3486
Fragments arranged by size:
3486 1308 1156
ApoI r'AATT_y
Cuts at: 1493 3259 3485 3637 5746 1493
Size: 1766 226 152 2109 1697
Fragments arranged by size:
2109 1766 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4504 160
Size: 872 3472 1606
Fragments arranged by size:
3472 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
AvaI C'yCGr_G
Cuts at: 1870 1943 2315 3014 3275 1870
Size: 73 372 699 261 4545
Fragments arranged by size:
4545 699 372 261 73
BamHI G'GATC_C
Cuts at: 3280 3647 3280
Size: 367 5583
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2845 3271 4726 618
Size: 325 217 627 1058 426 1455 1842
Fragments arranged by size:
1842 1455 1058 627 426 325 217
BanII G_rGCy'C
Cuts at: 1391 2143 2745 2835 3269 1391
Size: 752 602 90 434 4072
Fragments arranged by size:
4072 752 602 434 90
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2432 928
Size: 971 533 4446
Fragments arranged by size:
4446 971 533
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4141 4175 1903
Size: 34 50 34 2120 34 3678
Fragments arranged by size:
3678 2120 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3842 5052 3842
Size: 1210 4740
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5950
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2558 4561 136
Size: 107 122 71 631 605 886 2003 1525
Fragments arranged by size:
2003 1525 886 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5945 5945
Size: 5950
BlpI GC'TnA_GC
Cuts at: 3367 3367
Size: 5950
Bme1580I G_kGCm'C
Cuts at: 1790 2143 2703 2828 2850 4011 5167 1790
Size: 353 560 125 22 1161 1156 2573
Fragments arranged by size:
2573 1161 1156 560 353 125 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5950
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3681 4639 442
Size: 416 53 83 399 24 2264 958 1753
Fragments arranged by size:
2264 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5950
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2230 2230
Size: 5950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2615 3179 3260 4610 1239
Size: 1376 564 81 1350 2579
Fragments arranged by size:
2579 1376 1350 564 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4075 4940 5238 4075
Size: 865 298 4787
Fragments arranged by size:
4787 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2990 4613 882
Size: 617 1491 1623 2219
Fragments arranged by size:
2219 1623 1491 617
BsaAI yAC'GTr
Cuts at: 493 1204 3741 493
Size: 711 2537 2702
Fragments arranged by size:
2702 2537 711
BsaBI GATnn'nnATC
Cuts at: 5944 5944
Size: 5950
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2787 3757 4139 4774
Size: 53 83 186 936 1254 970 382 635
Cuts at: 4774 275
Size: 1451
Fragments arranged by size:
1451 1254 970 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2628 2842 4383 4781 5055 5202 5613 2628
Size: 214 1541 398 274 147 411 2965
Fragments arranged by size:
2965 1541 411 398 274 214 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2141 2171 2699 2729 1178
Size: 30 165 30 738 30 528 30 4399
Fragments arranged by size:
4399 738 528 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2165 2315 2333 1186
Size: 949 30 150 18 4803
Fragments arranged by size:
4803 949 150 30 18
BseYI C'CCAG_C
Cuts at: 2010 5153 5649 2010
Size: 3143 496 2311
Fragments arranged by size:
3143 2311 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2059 2195 2930 3071 1163
Size: 573 323 136 735 141 4042
Fragments arranged by size:
4042 735 573 323 141 136
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2869 3400 4161 5189 1540
Size: 275 186 868 531 761 1028 2301
Fragments arranged by size:
2301 1028 868 761 531 275 186
BsiHKAI G_wGCw'C
Cuts at: 2628 2703 2745 2822 4011 4096 5167 2628
Size: 75 42 77 1189 85 1071 3411
Fragments arranged by size:
3411 1189 1071 85 77 75 42
BsmI GAATG_Cn'
Cuts at: 3461 3554 3461
Size: 93 5857
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2143 2628 2703 2745 2822 2828 2835
Size: 399 353 485 75 42 77 6 7
Cuts at: 2835 2850 3269 4011 4096 5167 1391
Size: 15 419 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 485 419 399 353 85
77 75 42 15 7 6
BspEI T'CCGG_A
Cuts at: 5613 5613
Size: 5950
BspHI T'CATG_A
Cuts at: 3839 5569 3839
Size: 1730 4220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2183 2497 3305 844
Size: 1339 314 808 3489
Fragments arranged by size:
3489 1339 808 314
BsrBI CCG'CTC
Cuts at: 1394 1823 2155 2566 2840 3019 3837 1394
Size: 429 332 411 274 179 818 3507
Fragments arranged by size:
3507 818 429 411 332 274 179
BsrDI GCAATG_nn'
Cuts at: 65 1248 2583 4445 4619 65
Size: 1183 1335 1862 174 1396
Fragments arranged by size:
1862 1396 1335 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2667 4594 4781 1700
Size: 110 857 1927 187 2869
Fragments arranged by size:
2869 1927 857 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5950
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5950
BssSI C'ACGA_G
Cuts at: 4010 5022 4010
Size: 1012 4938
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2981 794
Size: 2187 3763
BstBI TT'CG_AA
Cuts at: 4754 4754
Size: 5950
BstEII G'GTnAC_C
Cuts at: 1184 2875 1184
Size: 1691 4259
BstXI CCAn_nnnn'nTGG
Cuts at: 2971 2971
Size: 5950
BstYI r'GATC_y
Cuts at: 1972 3280 3647 4032 4049 5501 5587 5599 5945
Size: 1308 367 385 17 1452 86 12 346
Cuts at: 5945 1972
Size: 1977
Fragments arranged by size:
1977 1452 1308 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2572 2572
Size: 5950
Bsu36I CC'TnA_GG
Cuts at: 2219 2219
Size: 5950
BtgI C'CryG_G
Cuts at: 513 1065 2412 3144 513
Size: 552 1347 732 3319
Fragments arranged by size:
3319 1347 732 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3028 487
Size: 683 56 1802 3409
Fragments arranged by size:
3409 1802 683 56
BtsI GCAGTG_nn'
Cuts at: 2543 2729 3054 3466 4260 4280 2543
Size: 186 325 412 794 20 4213
Fragments arranged by size:
4213 794 412 325 186 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5950
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5915
DraI TTT'AAA
Cuts at: 3254 3254
Size: 5950
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4957 809
Size: 507 3641 1802
Fragments arranged by size:
3641 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2197 2494 2550
Size: 54 1425 50 275 18 367 297 56
Cuts at: 2550 3397 4286 5915 8
Size: 847 889 1629 43
Fragments arranged by size:
1629 1425 889 847 367 297 275 56
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3397 1537
Size: 275 1585 4090
Fragments arranged by size:
4090 1585 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2378 2510 3880 1353
Size: 1025 132 1370 3423
Fragments arranged by size:
3423 1370 1025 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2162 4537 4911 5057 387
Size: 1064 138 573 2375 374 146 1280
Fragments arranged by size:
2375 1280 1064 573 374 146 138
EcoICRI GAG'CTC
Cuts at: 2743 2743
Size: 5950
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2615 3179 3260 4013 4610 5397 5665
Size: 393 983 564 81 753 597 787 268
Cuts at: 5665 1239
Size: 1524
Fragments arranged by size:
1524 983 787 753 597 564 393 268
81
EcoNI CCTnn'n_nnAGG
Cuts at: 2297 2297
Size: 5950
EcoO109I rG'GnC_Cy
Cuts at: 2139 2223 2139
Size: 84 5866
EcoRI G'AATT_C
Cuts at: 3259 3637 3259
Size: 378 5572
FspI TGC'GCA
Cuts at: 2589 4456 2589
Size: 1867 4083
HaeII r_GCGC'y
Cuts at: 1678 2490 3101 5097 1678
Size: 812 611 1996 2531
Fragments arranged by size:
2531 1996 812 611
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4591 4623 4665 4697 1373
Size: 32 135 32 3019 32 42 32 2626
Fragments arranged by size:
3019 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3294 3540 669
Size: 988 281 1356 246 3079
Fragments arranged by size:
3079 1356 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3245 748
Size: 2497 3453
HpaI GTT'AAC
Cuts at: 3540 3540
Size: 5950
KpnI G_GTAC'C
Cuts at: 3275 3275
Size: 5950
MfeI C'AATT_G
Cuts at: 3549 3549
Size: 5950
MluI A'CGCG_T
Cuts at: 5826 5826
Size: 5950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5064 5248 1305
Size: 3759 184 2007
Fragments arranged by size:
3759 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2199 10
Size: 54 1425 343 367 3761
Fragments arranged by size:
3761 1425 367 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3047 3908 4267 4426 518
Size: 648 1881 861 359 159 2042
Fragments arranged by size:
2042 1881 861 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2083 2281 2593 2653 2884 3146 3366
Size: 408 69 198 312 60 231 262 220
Cuts at: 3366 4043 5191 5436 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 408 312 262 245 231
220 198 69 60
NcoI C'CATG_G
Cuts at: 513 1065 2412 513
Size: 552 1347 4051
Fragments arranged by size:
4051 1347 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5950
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5950
NotI GC'GGCC_GC
Cuts at: 3397 3397
Size: 5950
NspI r_CATG'y
Cuts at: 1132 1229 3308 4853 1132
Size: 97 2079 1545 2229
Fragments arranged by size:
2229 2079 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5950
PciI A'CATG_T
Cuts at: 4849 4849
Size: 5950
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5950
PfoI T'CCnGG_A
Cuts at: 3156 3168 3156
Size: 12 5938
PmeI GTTT'AAAC
Cuts at: 3254 3254
Size: 5950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4019 4051 5532 5564 4019
Size: 32 1481 32 4405
Fragments arranged by size:
4405 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5950
PsiI TTA'TAA
Cuts at: 3520 3520
Size: 5950
PspOMI G'GGCC_C
Cuts at: 2139 2139
Size: 5950
PstI C_TGCA'G
Cuts at: 830 2019 2082 2780 3302 830
Size: 1189 63 698 522 3478
Fragments arranged by size:
3478 1189 698 522 63
PvuI CG_AT'CG
Cuts at: 2001 2869 2001
Size: 868 5082
PvuII CAG'CTG
Cuts at: 2014 2281 2593 2653 2014
Size: 267 312 60 5311
Fragments arranged by size:
5311 312 267 60
SacI G_AGCT'C
Cuts at: 2745 2745
Size: 5950
SacII CC_GC'GG
Cuts at: 3147 3147
Size: 5950
SalI G'TCGA_C
Cuts at: 1936 3292 1936
Size: 1356 4594
SapI GCTCTTCn'nnn_
Cuts at: 2378 2378
Size: 5950
SbfI CC_TGCA'GG
Cuts at: 3302 3302
Size: 5950
ScaI AGT'ACT
Cuts at: 4198 5910 4198
Size: 1712 4238
SfcI C'TryA_G
Cuts at: 826 1045 2015 2078 2776 3298 4433 5114 5305
Size: 219 970 63 698 522 1135 681 191
Cuts at: 5305 826
Size: 1471
Fragments arranged by size:
1471 1135 970 698 681 522 219 191
63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5950
SmaI CCC'GGG
Cuts at: 1872 3277 1872
Size: 1405 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2315 4054 4955 5217 820
Size: 197 926 372 1739 901 262 1553
Fragments arranged by size:
1739 1553 926 901 372 262 197
SnaBI TAC'GTA
Cuts at: 493 3741 493
Size: 3248 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5950
SphI G_CATG'C
Cuts at: 1132 3308 1132
Size: 2176 3774
SspI AAT'ATT
Cuts at: 5 52 3874 5
Size: 47 3822 2081
Fragments arranged by size:
3822 2081 47
StuI AGG'CCT
Cuts at: 1517 2471 2645 1517
Size: 954 174 4822
Fragments arranged by size:
4822 954 174
StyI C'CwwG_G
Cuts at: 513 1065 2412 3389 513
Size: 552 1347 977 3074
Fragments arranged by size:
3074 1347 977 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2686 3977 3994 4147 4332 4777 325
Size: 1255 1106 1291 17 153 185 445 1498
Fragments arranged by size:
1498 1291 1255 1106 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4196 5908 96
Size: 274 80 33 51 1443 2219 1712 138
Fragments arranged by size:
2219 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2179 2211 2623 2655 3013 3045 3173 3205 5910
Size: 32 412 32 358 32 128 32 2705
Cuts at: 5910 5942 2179
Size: 32 2187
Fragments arranged by size:
2705 2187 412 358 128 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2375 1316
Size: 1059 4891
XbaI T'CTAG_A
Cuts at: 3286 3286
Size: 5950
XhoI C'TCGA_G
Cuts at: 1943 2315 1943
Size: 372 5578
XmaI C'CCGG_G
Cuts at: 1870 3275 1870
Size: 1405 4545
XmnI GAAnn'nnTTC
Cuts at: 4079 4079
Size: 5950
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3758 276
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI DraIII EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI