Restriction map for
pFN21AE0719
(Circular) (Six-base) MAPSORT of: pFN21AE0719.seq Check: 3077 from: 1 to: 5620
pFN21AE0719 5620 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3430 278
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2963 1937
Size: 1026 4594
Acc65I G'GTAC_C
Cuts at: 2941 2941
Size: 5620
AclI AA'CG_TT
Cuts at: 1563 3747 4120 1563
Size: 2184 373 3063
Fragments arranged by size:
3063 2184 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2120 2198 2285 2408 3683 5067 5335 1632
Size: 488 78 87 123 1275 1384 268 1917
Fragments arranged by size:
1917 1384 1275 488 268 123 87 78
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5423
AflIII A'CryG_T
Cuts at: 1203 4519 5496 1203
Size: 3316 977 1327
Fragments arranged by size:
3316 1327 977
AgeI A'CCGG_T
Cuts at: 4451 4451
Size: 5620
AhdI GACnn_n'nnGTC
Cuts at: 4349 4349
Size: 5620
AleI CACnn'nnGTG
Cuts at: 1166 2552 1166
Size: 1386 4234
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2804 1184
Size: 1620 4000
AlwNI CAG_nnn'CTG
Cuts at: 2263 2338 2401 3363 4935 2263
Size: 75 63 962 1572 2948
Fragments arranged by size:
2948 1572 962 75 63
ApaLI G'TGCA_C
Cuts at: 2213 3677 4833 2213
Size: 1464 1156 3000
Fragments arranged by size:
3000 1464 1156
ApoI r'AATT_y
Cuts at: 1493 2929 3155 3307 5416 1493
Size: 1436 226 152 2109 1697
Fragments arranged by size:
2109 1697 1436 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4174 160
Size: 872 3142 1606
Fragments arranged by size:
3142 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
AvaI C'yCGr_G
Cuts at: 1870 1943 2516 2687 2945 1870
Size: 73 573 171 258 4545
Fragments arranged by size:
4545 573 258 171 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2489 2522 2489
Size: 33 5587
BamHI G'GATC_C
Cuts at: 2950 3317 2950
Size: 367 5253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 2420 2464 2941 4396
Size: 325 217 627 255 378 44 477 1455
Cuts at: 4396 618
Size: 1842
Fragments arranged by size:
1842 1455 627 477 378 325 255 217
44
BanII G_rGCy'C
Cuts at: 1391 2301 2877 2939 1391
Size: 910 576 62 4072
Fragments arranged by size:
4072 910 576 62
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2682 928
Size: 971 783 3866
Fragments arranged by size:
3866 971 783
BbvCI CC'TCA_GC
Cuts at: 2252 2252
Size: 5620
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3811 3845 1903
Size: 34 1874 34 3678
Fragments arranged by size:
3678 1874 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3512 4722 3512
Size: 1210 4410
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5620
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4231 136
Size: 107 122 71 631 605 2559 1525
Fragments arranged by size:
2559 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2157 5615 2157
Size: 3458 2162
BlpI GC'TnA_GC
Cuts at: 3037 3037
Size: 5620
Bme1580I G_kGCm'C
Cuts at: 1790 2045 2217 2469 2763 3681 4837 1790
Size: 255 172 252 294 918 1156 2573
Fragments arranged by size:
2573 1156 918 294 255 252 172
BmgBI CAC'GTC
Cuts at: 1318 2209 1318
Size: 891 4729
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3351 4309 442
Size: 416 53 83 399 24 1934 958 1753
Fragments arranged by size:
1934 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2584 2789 4280 1239
Size: 1345 205 1491 2579
Fragments arranged by size:
2579 1491 1345 205
Bpu10I CC'TnA_GC
Cuts at: 2252 2871 2252
Size: 619 5001
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2153 3745 4610 4908 2153
Size: 1592 865 298 2865
Fragments arranged by size:
2865 1592 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2376 4283 882
Size: 617 877 1907 2219
Fragments arranged by size:
2219 1907 877 617
BsaAI yAC'GTr
Cuts at: 493 1204 3411 493
Size: 711 2207 2702
Fragments arranged by size:
2702 2207 711
BsaBI GATnn'nnATC
Cuts at: 5614 5614
Size: 5620
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3427 3809 4444 275
Size: 53 83 186 936 1894 382 635 1451
Fragments arranged by size:
1894 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4053 4451 4725 4872 5283 4053
Size: 398 274 147 411 4390
Fragments arranged by size:
4390 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2264 2294 2557 2587 1178
Size: 30 165 30 861 30 263 30 4211
Fragments arranged by size:
4211 861 263 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5620
BseYI C'CCAG_C
Cuts at: 2659 2669 2697 2816 2881 4823 5319 2659
Size: 10 28 119 65 1942 496 2960
Fragments arranged by size:
2960 1942 496 119 65 28 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2291 1163
Size: 573 555 4492
Fragments arranged by size:
4492 573 555
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3070 3831 4859 1540
Size: 275 186 1069 761 1028 2301
Fragments arranged by size:
2301 1069 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2217 2301 3681 3766 4837 2217
Size: 84 1380 85 1071 3000
Fragments arranged by size:
3000 1380 1071 85 84
BsmI GAATG_Cn'
Cuts at: 3131 3224 3131
Size: 93 5527
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2217 2301 2469 2763 2877 2939
Size: 399 255 172 84 168 294 114 62
Cuts at: 2939 3681 3766 4837 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 294 255 172 168
114 85 84 62
BspEI T'CCGG_A
Cuts at: 5283 5283
Size: 5620
BspHI T'CATG_A
Cuts at: 3509 5239 3509
Size: 1730 3890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2409 2796 2975 844
Size: 1565 387 179 3489
Fragments arranged by size:
3489 1565 387 179
BsrBI CCG'CTC
Cuts at: 1394 1823 3507 1394
Size: 429 1684 3507
Fragments arranged by size:
3507 1684 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4115 4289 65
Size: 1183 2867 174 1396
Fragments arranged by size:
2867 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4264 4451 1700
Size: 110 2454 187 2869
Fragments arranged by size:
2869 2454 187 110
BsrGI T'GTAC_A
Cuts at: 96 2058 96
Size: 1962 3658
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5620
BssSI C'ACGA_G
Cuts at: 3680 4692 3680
Size: 1012 4608
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2574 2803 794
Size: 1780 229 3611
Fragments arranged by size:
3611 1780 229
BstBI TT'CG_AA
Cuts at: 4424 4424
Size: 5620
BstEII G'GTnAC_C
Cuts at: 1184 2692 1184
Size: 1508 4112
BstXI CCAn_nnnn'nTGG
Cuts at: 2431 2554 2431
Size: 123 5497
BstYI r'GATC_y
Cuts at: 1972 2157 2950 3317 3702 3719 5171 5257 5269
Size: 185 793 367 385 17 1452 86 12
Cuts at: 5269 5615 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 793 385 367 346 185 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4129
Fragments arranged by size:
4129 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4881
Fragments arranged by size:
4881 683 56
BtsI GCAGTG_nn'
Cuts at: 2142 3136 3930 3950 2142
Size: 994 794 20 3812
Fragments arranged by size:
3812 994 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5620
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5585
DraI TTT'AAA
Cuts at: 2924 2924
Size: 5620
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4627 809
Size: 507 3311 1802
Fragments arranged by size:
3311 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3067 3956 5585
Size: 54 1425 50 275 18 1237 889 1629
Cuts at: 5585 8
Size: 43
Fragments arranged by size:
1629 1425 1237 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3067 1537
Size: 275 1255 4090
Fragments arranged by size:
4090 1255 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2333 2745 3550 1353
Size: 980 412 805 3423
Fragments arranged by size:
3423 980 805 412
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2903 4207 4581 4727 387
Size: 1064 138 1314 1304 374 146 1280
Fragments arranged by size:
1314 1304 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2299 2299
Size: 5620
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 2198 2285 2408 2584 2789 3683
Size: 393 488 78 87 123 176 205 894
Cuts at: 3683 4280 5067 5335 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 894 787 597 488 393 268 205
176 123 87 78
EcoNI CCTnn'n_nnAGG
Cuts at: 2255 2255
Size: 5620
EcoO109I rG'GnC_Cy
Cuts at: 2134 2260 2785 2134
Size: 126 525 4969
Fragments arranged by size:
4969 525 126
EcoRI G'AATT_C
Cuts at: 2929 3307 2929
Size: 378 5242
EcoRV GAT'ATC
Cuts at: 2560 2560
Size: 5620
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2737 2737
Size: 5620
FspI TGC'GCA
Cuts at: 2474 4126 2474
Size: 1652 3968
HaeII r_GCGC'y
Cuts at: 1678 4767 1678
Size: 3089 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2581 2613 4261 4293 4335
Size: 32 135 32 1009 32 1648 32 42
Cuts at: 4335 4367 1373
Size: 32 2626
Fragments arranged by size:
2626 1648 1009 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2964 3210 669
Size: 988 281 1026 246 3079
Fragments arranged by size:
3079 1026 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5620
HpaI GTT'AAC
Cuts at: 3210 3210
Size: 5620
KpnI G_GTAC'C
Cuts at: 2945 2945
Size: 5620
MfeI C'AATT_G
Cuts at: 3219 3219
Size: 5620
MluI A'CGCG_T
Cuts at: 5496 5496
Size: 5620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2221 4734 4918 1305
Size: 916 2513 184 2007
Fragments arranged by size:
2513 2007 916 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3798
Fragments arranged by size:
3798 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2552 3578 3937 4096 518
Size: 648 843 543 1026 359 159 2042
Fragments arranged by size:
2042 1026 843 648 543 359 159
MspA1I CmG'CkG
Cuts at: 1606 2089 2335 2552 2701 2824 2885 3036 3713
Size: 483 246 217 149 123 61 151 677
Cuts at: 3713 4861 5106 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 483 246 245 217 151
149 123 61
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4129
Fragments arranged by size:
4129 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5620
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5620
NotI GC'GGCC_GC
Cuts at: 3067 3067
Size: 5620
NspI r_CATG'y
Cuts at: 1132 1229 2978 4523 1132
Size: 97 1749 1545 2229
Fragments arranged by size:
2229 1749 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5620
PciI A'CATG_T
Cuts at: 4519 4519
Size: 5620
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2491 1108
Size: 1383 4237
PmeI GTTT'AAAC
Cuts at: 2924 2924
Size: 5620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 5202 5234 3689
Size: 32 1481 32 4075
Fragments arranged by size:
4075 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2134 2260 2785 2134
Size: 126 525 4969
Fragments arranged by size:
4969 525 126
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5620
PsiI TTA'TAA
Cuts at: 3190 3190
Size: 5620
PstI C_TGCA'G
Cuts at: 830 2343 2808 2907 2972 830
Size: 1513 465 99 65 3478
Fragments arranged by size:
3478 1513 465 99 65
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5620
PvuII CAG'CTG
Cuts at: 2089 2335 2552 2701 2885 2089
Size: 246 217 149 184 4824
Fragments arranged by size:
4824 246 217 184 149
SacI G_AGCT'C
Cuts at: 2301 2301
Size: 5620
SalI G'TCGA_C
Cuts at: 1936 2962 1936
Size: 1026 4594
SanDI GG'GwC_CC
Cuts at: 2134 2134
Size: 5620
SbfI CC_TGCA'GG
Cuts at: 2972 2972
Size: 5620
ScaI AGT'ACT
Cuts at: 3868 5580 3868
Size: 1712 3908
SfcI C'TryA_G
Cuts at: 826 1045 2339 2804 2903 2968 4103 4784 4975
Size: 219 1294 465 99 65 1135 681 191
Cuts at: 4975 826
Size: 1471
Fragments arranged by size:
1471 1294 1135 681 465 219 191 99
65
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5620
SmaI CCC'GGG
Cuts at: 1872 2689 2947 1872
Size: 817 258 4545
Fragments arranged by size:
4545 817 258
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2168 3724 4625 4887 820
Size: 197 926 225 1556 901 262 1553
Fragments arranged by size:
1556 1553 926 901 262 225 197
SnaBI TAC'GTA
Cuts at: 493 3411 493
Size: 2918 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5620
SphI G_CATG'C
Cuts at: 1132 2978 1132
Size: 1846 3774
SspI AAT'ATT
Cuts at: 5 52 3544 5
Size: 47 3492 2081
Fragments arranged by size:
3492 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5620
StyI C'CwwG_G
Cuts at: 513 1065 2004 3059 513
Size: 552 939 1055 3074
Fragments arranged by size:
3074 1055 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2408 2498 2683 2711 3647 3664 3817
Size: 1255 828 90 185 28 936 17 153
Cuts at: 3817 4002 4447 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 936 828 445 185 185 153
90 28 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2058 2271 3866
Size: 274 80 33 51 1443 81 213 1595
Cuts at: 3866 5578 96
Size: 1712 138
Fragments arranged by size:
1712 1595 1443 274 213 138 81 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5580 5612 5580
Size: 32 5588
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5620
XbaI T'CTAG_A
Cuts at: 2956 2956
Size: 5620
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2743 2842 2743
Size: 99 5521
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5620
XmaI C'CCGG_G
Cuts at: 1870 2687 2945 1870
Size: 817 258 4545
Fragments arranged by size:
4545 817 258
XmnI GAAnn'nnTTC
Cuts at: 3749 3749
Size: 5620
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3428 276
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI
SfiI SfoI SrfI SwaI