Restriction map for
pFN21AE0718
(Circular) (Six-base) MAPSORT of: pFN21AE0718.seq Check: 6021 from: 1 to: 5947
pFN21AE0718 5947 bp
With 182 enzymes: SgfI *
March 28, 2012 12:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3757 278
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3290 1937
Size: 1353 4594
Acc65I G'GTAC_C
Cuts at: 2678 3268 2678
Size: 590 5357
AclI AA'CG_TT
Cuts at: 1563 4074 4447 1563
Size: 2511 373 3063
Fragments arranged by size:
3063 2511 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2943 2961 4010 5394 5662 1632
Size: 1311 18 1049 1384 268 1917
Fragments arranged by size:
1917 1384 1311 1049 268 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5750
AflIII A'CryG_T
Cuts at: 1203 4846 5823 1203
Size: 3643 977 1327
Fragments arranged by size:
3643 1327 977
AgeI A'CCGG_T
Cuts at: 4778 4778
Size: 5947
AhdI GACnn_n'nnGTC
Cuts at: 4676 4676
Size: 5947
AleI CACnn'nnGTG
Cuts at: 1166 2541 1166
Size: 1375 4572
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5947
AlwNI CAG_nnn'CTG
Cuts at: 3690 5262 3690
Size: 1572 4375
ApaLI G'TGCA_C
Cuts at: 4004 5160 4004
Size: 1156 4791
ApoI r'AATT_y
Cuts at: 1493 2216 2261 2703 2839 2861 3200 3225 3236
Size: 723 45 442 136 22 339 25 11
Cuts at: 3236 3256 3482 3634 5743 1493
Size: 20 226 152 2109 1697
Fragments arranged by size:
2109 1697 723 442 339 226 152 136
45 25 22 20 11
AseI AT'TA_AT
Cuts at: 160 1032 2370 3060 4501 160
Size: 872 1338 690 1441 1606
Fragments arranged by size:
1606 1441 1338 872 690
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
AvaI C'yCGr_G
Cuts at: 1870 1943 2062 2191 3272 1870
Size: 73 119 129 1081 4545
Fragments arranged by size:
4545 1081 129 119 73
BamHI G'GATC_C
Cuts at: 3277 3644 3277
Size: 367 5580
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 2678 3268 4723 618
Size: 325 217 627 261 630 590 1455 1842
Fragments arranged by size:
1842 1455 630 627 590 325 261 217
BanII G_rGCy'C
Cuts at: 1391 2166 2664 3266 1391
Size: 775 498 602 4072
Fragments arranged by size:
4072 775 602 498
BbeI G_GCGC'C
Cuts at: 2052 2052
Size: 5947
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4976
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4138 4172 1903
Size: 34 2201 34 3678
Fragments arranged by size:
3678 2201 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3061 3839 5049 3061
Size: 778 1210 3959
Fragments arranged by size:
3959 1210 778
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5947
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2073 2098 4558
Size: 107 122 71 631 605 401 25 2460
Cuts at: 4558 136
Size: 1525
Fragments arranged by size:
2460 1525 631 605 401 122 107 71
25
BglII A'GATC_T
Cuts at: 5942 5942
Size: 5947
BlpI GC'TnA_GC
Cuts at: 3364 3364
Size: 5947
Bme1580I G_kGCm'C
Cuts at: 1790 2192 4008 5164 1790
Size: 402 1816 1156 2573
Fragments arranged by size:
2573 1816 1156 402
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5947
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3678 4636 442
Size: 416 53 83 399 24 2261 958 1753
Fragments arranged by size:
2261 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4607 1239
Size: 3368 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4072 4937 5235 4072
Size: 865 298 4784
Fragments arranged by size:
4784 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4610 882
Size: 617 3111 2219
Fragments arranged by size:
3111 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3738 493
Size: 711 2534 2702
Fragments arranged by size:
2702 2534 711
BsaBI GATnn'nnATC
Cuts at: 5941 5941
Size: 5947
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2049 3754 4136 4771
Size: 53 83 186 936 516 1705 382 635
Cuts at: 4771 275
Size: 1451
Fragments arranged by size:
1705 1451 936 635 516 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4380 4778 5052 5199 5610 4380
Size: 398 274 147 411 4717
Fragments arranged by size:
4717 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5722
Fragments arranged by size:
5722 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2029 1186
Size: 843 5104
BseYI C'CCAG_C
Cuts at: 2203 2429 5150 5646 2203
Size: 226 2721 496 2504
Fragments arranged by size:
2721 2504 496 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5374
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2102 3397 4158 5186 1540
Size: 275 186 101 1295 761 1028 2301
Fragments arranged by size:
2301 1295 1028 761 275 186 101
BsiHKAI G_wGCw'C
Cuts at: 2664 4008 4093 5164 2664
Size: 1344 85 1071 3447
Fragments arranged by size:
3447 1344 1071 85
BsmI GAATG_Cn'
Cuts at: 3458 3551 3458
Size: 93 5854
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2166 2192 2664 3266 4008 4093 5164
Size: 399 376 26 472 602 742 85 1071
Cuts at: 5164 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 602 472 399 376 85
26
BspEI T'CCGG_A
Cuts at: 5610 5610
Size: 5947
BspHI T'CATG_A
Cuts at: 2277 2323 3836 5566 2277
Size: 46 1513 1730 2658
Fragments arranged by size:
2658 1730 1513 46
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3302 844
Size: 2458 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2088 3834 1394
Size: 429 265 1746 3507
Fragments arranged by size:
3507 1746 429 265
BsrDI GCAATG_nn'
Cuts at: 65 1248 2806 4442 4616 65
Size: 1183 1558 1636 174 1396
Fragments arranged by size:
1636 1558 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2097 4591 4778 1700
Size: 110 287 2494 187 2869
Fragments arranged by size:
2869 2494 287 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5947
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5947
BssSI C'ACGA_G
Cuts at: 2541 4007 5019 2541
Size: 1466 1012 3469
Fragments arranged by size:
3469 1466 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5947
BstBI TT'CG_AA
Cuts at: 4751 4751
Size: 5947
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5947
BstYI r'GATC_y
Cuts at: 1972 3277 3644 4029 4046 5498 5584 5596 5942
Size: 1305 367 385 17 1452 86 12 346
Cuts at: 5942 1972
Size: 1977
Fragments arranged by size:
1977 1452 1305 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2056 2056
Size: 5947
BtgI C'CryG_G
Cuts at: 513 1065 2122 2148 513
Size: 552 1057 26 4312
Fragments arranged by size:
4312 1057 552 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5208
Fragments arranged by size:
5208 683 56
BtsI GCAGTG_nn'
Cuts at: 2229 2934 3463 4257 4277 2229
Size: 705 529 794 20 3899
Fragments arranged by size:
3899 794 705 529 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5947
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5912
DraI TTT'AAA
Cuts at: 2479 3199 3251 2479
Size: 720 52 5175
Fragments arranged by size:
5175 720 52
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4954 809
Size: 507 3638 1802
Fragments arranged by size:
3638 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2099 3394 4283
Size: 54 1425 50 275 18 269 1295 889
Cuts at: 4283 5912 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1295 889 275 269 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2099 3394 1537
Size: 275 287 1295 4090
Fragments arranged by size:
4090 1295 287 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2652 3102 3877 1353
Size: 1299 450 775 3423
Fragments arranged by size:
3423 1299 775 450
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4534 4908 5054 387
Size: 1064 138 2945 374 146 1280
Fragments arranged by size:
2945 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2662 2662
Size: 5947
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2943 2961 4010 4607 5394 5662 1239
Size: 393 1311 18 1049 597 787 268 1524
Fragments arranged by size:
1524 1311 1049 787 597 393 268 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2793 2793
Size: 5947
EcoRI G'AATT_C
Cuts at: 3256 3634 3256
Size: 378 5569
FspI TGC'GCA
Cuts at: 2156 4453 2156
Size: 2297 3650
FspAI rTGC'GCAy
Cuts at: 2156 2156
Size: 5947
HaeII r_GCGC'y
Cuts at: 1678 2052 5094 1678
Size: 374 3042 2531
Fragments arranged by size:
3042 2531 374
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2381 2413 2858 2890 3225
Size: 32 135 32 809 32 445 32 335
Cuts at: 3225 3257 4588 4620 4662 4694 1373
Size: 32 1331 32 42 32 2626
Fragments arranged by size:
2626 1331 809 445 335 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2133 2586 2760 2967 3291 3537
Size: 988 281 195 453 174 207 324 246
Cuts at: 3537 669
Size: 3079
Fragments arranged by size:
3079 988 453 324 281 246 207 195
174
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5947
HpaI GTT'AAC
Cuts at: 2760 3537 2760
Size: 777 5170
KasI G'GCGC_C
Cuts at: 2048 2048
Size: 5947
KpnI G_GTAC'C
Cuts at: 2682 3272 2682
Size: 590 5357
MfeI C'AATT_G
Cuts at: 3546 3546
Size: 5947
MluI A'CGCG_T
Cuts at: 5823 5823
Size: 5947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2288 5061 5245 1305
Size: 983 2773 184 2007
Fragments arranged by size:
2773 2007 983 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4125
Fragments arranged by size:
4125 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2541 2753 3905 4264 4423 518
Size: 648 1375 212 1152 359 159 2042
Fragments arranged by size:
2042 1375 1152 648 359 212 159
MspA1I CmG'CkG
Cuts at: 1606 2031 2124 3363 4040 5188 5433 1606
Size: 425 93 1239 677 1148 245 2120
Fragments arranged by size:
2120 1239 1148 677 425 245 93
NaeI GCC'GGC
Cuts at: 2099 2099
Size: 5947
NarI GG'CG_CC
Cuts at: 2049 2049
Size: 5947
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5395
NdeI CA'TA_TG
Cuts at: 387 2764 387
Size: 2377 3570
NgoMIV G'CCGG_C
Cuts at: 2097 2097
Size: 5947
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5947
NotI GC'GGCC_GC
Cuts at: 3394 3394
Size: 5947
NspI r_CATG'y
Cuts at: 1132 1229 3305 4850 1132
Size: 97 2076 1545 2229
Fragments arranged by size:
2229 2076 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5947
PciI A'CATG_T
Cuts at: 4846 4846
Size: 5947
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5947
PfoI T'CCnGG_A
Cuts at: 2169 3229 2169
Size: 1060 4887
PmeI GTTT'AAAC
Cuts at: 3251 3251
Size: 5947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4016 4048 5529 5561 4016
Size: 32 1481 32 4402
Fragments arranged by size:
4402 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5947
PsiI TTA'TAA
Cuts at: 3517 3517
Size: 5947
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3201 3233 3201
Size: 32 5915
PstI C_TGCA'G
Cuts at: 830 3299 830
Size: 2469 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5947
SacI G_AGCT'C
Cuts at: 2664 2664
Size: 5947
SacII CC_GC'GG
Cuts at: 2125 2125
Size: 5947
SalI G'TCGA_C
Cuts at: 1936 3289 1936
Size: 1353 4594
SapI GCTCTTCn'nnn_
Cuts at: 2652 2652
Size: 5947
SbfI CC_TGCA'GG
Cuts at: 3299 3299
Size: 5947
ScaI AGT'ACT
Cuts at: 4195 5907 4195
Size: 1712 4235
SfcI C'TryA_G
Cuts at: 826 1045 2179 3295 4430 5111 5302 826
Size: 219 1134 1116 1135 681 191 1471
Fragments arranged by size:
1471 1135 1134 1116 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2073 2098 2073
Size: 25 5922
SfoI GGC'GCC
Cuts at: 2050 2050
Size: 5947
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5947
SmaI CCC'GGG
Cuts at: 1872 2193 3274 1872
Size: 321 1081 4545
Fragments arranged by size:
4545 1081 321
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4051 4952 5214 820
Size: 197 926 2108 901 262 1553
Fragments arranged by size:
2108 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3738 493
Size: 3245 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5947
SphI G_CATG'C
Cuts at: 1132 3305 1132
Size: 2173 3774
SrfI GCCC'GGGC
Cuts at: 2193 2193
Size: 5947
SspI AAT'ATT
Cuts at: 5 52 2578 2643 3871 5
Size: 47 2526 65 1228 2081
Fragments arranged by size:
2526 2081 1228 65 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5947
StyI C'CwwG_G
Cuts at: 513 1065 2360 3386 513
Size: 552 1295 1026 3074
Fragments arranged by size:
3074 1295 1026 552
SwaI ATTT'AAAT
Cuts at: 3199 3199
Size: 5947
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2594 3974 3991 4144 4329 4774 325
Size: 1255 1014 1380 17 153 185 445 1498
Fragments arranged by size:
1498 1380 1255 1014 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2335 4193 5905
Size: 274 80 33 51 1443 358 1858 1712
Cuts at: 5905 96
Size: 138
Fragments arranged by size:
1858 1712 1443 358 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5907 5939 5907
Size: 32 5915
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5947
XbaI T'CTAG_A
Cuts at: 3283 3283
Size: 5947
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5947
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5947
XmaI C'CCGG_G
Cuts at: 1870 2191 3272 1870
Size: 321 1081 4545
Fragments arranged by size:
4545 1081 321
XmnI GAAnn'nnTTC
Cuts at: 2319 2523 4076 2319
Size: 204 1553 4190
Fragments arranged by size:
4190 1553 204
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3755 276
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PsrI PstI PvuI SacI SacII
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoO109I EcoRV FalI FseI NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PvuII RsrII SanDI SexAI