Restriction map for
pFN21AE0714
(Circular) (Six-base) MAPSORT of: pFN21AE0714.seq Check: 1197 from: 1 to: 5938
pFN21AE0714 5938 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2023 2023
Size: 5938
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3748 278
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3281 1937
Size: 1344 4594
Acc65I G'GTAC_C
Cuts at: 3259 3259
Size: 5938
AclI AA'CG_TT
Cuts at: 1563 4065 4438 1563
Size: 2502 373 3063
Fragments arranged by size:
3063 2502 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4001 5385 5653 1632
Size: 2369 1384 268 1917
Fragments arranged by size:
2369 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2866 820
Size: 197 1849 3892
Fragments arranged by size:
3892 1849 197
AflIII A'CryG_T
Cuts at: 1203 4837 5814 1203
Size: 3634 977 1327
Fragments arranged by size:
3634 1327 977
AgeI A'CCGG_T
Cuts at: 4769 4769
Size: 5938
AhdI GACnn_n'nnGTC
Cuts at: 4667 4667
Size: 5938
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5938
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5938
AlwNI CAG_nnn'CTG
Cuts at: 2156 2291 3681 5253 2156
Size: 135 1390 1572 2841
Fragments arranged by size:
2841 1572 1390 135
ApaI G_GGCC'C
Cuts at: 2180 2180
Size: 5938
ApaLI G'TGCA_C
Cuts at: 3995 5151 3995
Size: 1156 4782
ApoI r'AATT_y
Cuts at: 1493 3247 3473 3625 5734 1493
Size: 1754 226 152 2109 1697
Fragments arranged by size:
2109 1754 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4492 160
Size: 872 3460 1606
Fragments arranged by size:
3460 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
AvaI C'yCGr_G
Cuts at: 1870 1943 2067 2182 3263 1870
Size: 73 124 115 1081 4545
Fragments arranged by size:
4545 1081 124 115 73
BamHI G'GATC_C
Cuts at: 3098 3268 3635 3098
Size: 170 367 5401
Fragments arranged by size:
5401 367 170
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3259 4714 618
Size: 325 217 627 1472 1455 1842
Fragments arranged by size:
1842 1472 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2128 2180 2257 3017 3257 1391
Size: 737 52 77 760 240 4072
Fragments arranged by size:
4072 760 737 240 77 52
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4967
BbvCI CC'TCA_GC
Cuts at: 2169 2169
Size: 5938
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4129 4163 1903
Size: 34 50 34 2108 34 3678
Fragments arranged by size:
3678 2108 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3830 5040 3830
Size: 1210 4728
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5938
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4549 136
Size: 107 122 71 631 605 2877 1525
Fragments arranged by size:
2877 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5933 5933
Size: 5938
BlpI GC'TnA_GC
Cuts at: 2009 3355 2009
Size: 1346 4592
Bme1580I G_kGCm'C
Cuts at: 1790 2180 3999 5155 1790
Size: 390 1819 1156 2573
Fragments arranged by size:
2573 1819 1156 390
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5938
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2423 3040 3669
Size: 416 53 83 399 24 1006 617 629
Cuts at: 3669 4627 442
Size: 958 1753
Fragments arranged by size:
1753 1006 958 629 617 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5938
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3118 3208 3118
Size: 90 5848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 4598 1239
Size: 827 2532 2579
Fragments arranged by size:
2579 2532 827
Bpu10I CC'TnA_GC
Cuts at: 2169 2932 2944 2169
Size: 763 12 5163
Fragments arranged by size:
5163 763 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4063 4928 5226 4063
Size: 865 298 4775
Fragments arranged by size:
4775 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3174 4601 882
Size: 617 1675 1427 2219
Fragments arranged by size:
2219 1675 1427 617
BsaAI yAC'GTr
Cuts at: 493 1204 3729 493
Size: 711 2525 2702
Fragments arranged by size:
2702 2525 711
BsaBI GATnn'nnATC
Cuts at: 5932 5932
Size: 5938
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3745 4127 4762 275
Size: 53 83 186 936 2212 382 635 1451
Fragments arranged by size:
2212 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4371 4769 5043 5190 5601 4371
Size: 398 274 147 411 4708
Fragments arranged by size:
4708 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5713
Fragments arranged by size:
5713 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2136 3197 1186
Size: 950 1061 3927
Fragments arranged by size:
3927 1061 950
BseYI C'CCAG_C
Cuts at: 2094 2269 2320 2374 2398 2482 2813 2818 2956
Size: 175 51 54 24 84 331 5 138
Cuts at: 2956 2986 2992 5141 5637 2094
Size: 30 6 2149 496 2395
Fragments arranged by size:
2395 2149 496 331 175 138 84 54
51 30 24 6 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2269 1163
Size: 573 533 4832
Fragments arranged by size:
4832 573 533
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3388 4149 5177 1540
Size: 275 186 1387 761 1028 2301
Fragments arranged by size:
2301 1387 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3999 4084 5155 3999
Size: 85 1071 4782
Fragments arranged by size:
4782 1071 85
BsmI GAATG_Cn'
Cuts at: 3449 3542 3449
Size: 93 5845
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2128 2180 2257 3017 3257 3999 4084
Size: 399 338 52 77 760 240 742 85
Cuts at: 4084 5155 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 760 742 399 338 240 85
77 52
BspEI T'CCGG_A
Cuts at: 5601 5601
Size: 5938
BspHI T'CATG_A
Cuts at: 3827 5557 3827
Size: 1730 4208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2023 3293 844
Size: 1179 1270 3489
Fragments arranged by size:
3489 1270 1179
BsrBI CCG'CTC
Cuts at: 1394 1823 2074 3825 1394
Size: 429 251 1751 3507
Fragments arranged by size:
3507 1751 429 251
BsrDI GCAATG_nn'
Cuts at: 65 1248 4433 4607 65
Size: 1183 3185 174 1396
Fragments arranged by size:
3185 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4582 4769 1700
Size: 110 2772 187 2869
Fragments arranged by size:
2869 2772 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5938
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5938
BssSI C'ACGA_G
Cuts at: 3998 5010 3998
Size: 1012 4926
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2029 2156 794
Size: 1235 127 4576
Fragments arranged by size:
4576 1235 127
BstBI TT'CG_AA
Cuts at: 4742 4742
Size: 5938
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5938
BstYI r'GATC_y
Cuts at: 1972 2328 2382 2436 2826 3098 3268 3635 4020
Size: 356 54 54 390 272 170 367 385
Cuts at: 4020 4037 5489 5575 5587 5933 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 390 385 367 356 346 272
170 86 54 54 17 12
Bsu36I CC'TnA_GG
Cuts at: 2015 2015
Size: 5938
BtgI C'CryG_G
Cuts at: 513 1065 2452 513
Size: 552 1387 3999
Fragments arranged by size:
3999 1387 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5199
Fragments arranged by size:
5199 683 56
BtsI GCAGTG_nn'
Cuts at: 3454 4248 4268 3454
Size: 794 20 5124
Fragments arranged by size:
5124 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5938
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5903
DraI TTT'AAA
Cuts at: 3242 3242
Size: 5938
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4945 809
Size: 507 3629 1802
Fragments arranged by size:
3629 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2875 3385 4274
Size: 54 1425 50 275 18 1045 510 889
Cuts at: 4274 5903 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1045 889 510 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3385 1537
Size: 275 1573 4090
Fragments arranged by size:
4090 1573 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3868 1353
Size: 2515 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2216 4525 4899 5045 387
Size: 1064 138 627 2309 374 146 1280
Fragments arranged by size:
2309 1280 1064 627 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 4001 4598 5385 5653 1239
Size: 393 434 1935 597 787 268 1524
Fragments arranged by size:
1935 1524 787 597 434 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2225 2225
Size: 5938
EcoO109I rG'GnC_Cy
Cuts at: 2176 2177 2288 2858 3215 2176
Size: 1 111 570 357 4899
Fragments arranged by size:
4899 570 357 111 1
EcoRI G'AATT_C
Cuts at: 3247 3625 3247
Size: 378 5560
FspI TGC'GCA
Cuts at: 4444 4444
Size: 5938
HaeII r_GCGC'y
Cuts at: 1678 5085 1678
Size: 3407 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3090 3122 4579 4611 4653
Size: 32 135 32 1518 32 1457 32 42
Cuts at: 4653 4685 1373
Size: 32 2626
Fragments arranged by size:
2626 1518 1457 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3282 3528 669
Size: 988 281 1344 246 3079
Fragments arranged by size:
3079 1344 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5938
HpaI GTT'AAC
Cuts at: 3528 3528
Size: 5938
KpnI G_GTAC'C
Cuts at: 3263 3263
Size: 5938
MfeI C'AATT_G
Cuts at: 3537 3537
Size: 5938
MluI A'CGCG_T
Cuts at: 5814 5814
Size: 5938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3051 5052 5236 1305
Size: 1746 2001 184 2007
Fragments arranged by size:
2007 2001 1746 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4116
Fragments arranged by size:
4116 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3896 4255 4414 518
Size: 648 2730 359 159 2042
Fragments arranged by size:
2730 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2029 2173 2198 2273 3354 4031 5179 5424
Size: 423 144 25 75 1081 677 1148 245
Cuts at: 5424 1606
Size: 2120
Fragments arranged by size:
2120 1148 1081 677 423 245 144 75
25
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5386
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5938
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5938
NotI GC'GGCC_GC
Cuts at: 3385 3385
Size: 5938
NspI r_CATG'y
Cuts at: 1132 1229 3296 4841 1132
Size: 97 2067 1545 2229
Fragments arranged by size:
2229 2067 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2105 2243 1348
Size: 757 138 5043
Fragments arranged by size:
5043 757 138
PciI A'CATG_T
Cuts at: 4837 4837
Size: 5938
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2291 2345 2453 1108
Size: 1183 54 108 4593
Fragments arranged by size:
4593 1183 108 54
PmeI GTTT'AAAC
Cuts at: 3242 3242
Size: 5938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4007 4039 5520 5552 4007
Size: 32 1481 32 4393
Fragments arranged by size:
4393 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2858 2858
Size: 5938
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5938
PsiI TTA'TAA
Cuts at: 3508 3508
Size: 5938
PspOMI G'GGCC_C
Cuts at: 2176 2176
Size: 5938
PstI C_TGCA'G
Cuts at: 830 2034 2152 2219 3290 830
Size: 1204 118 67 1071 3478
Fragments arranged by size:
3478 1204 1071 118 67
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5938
PvuII CAG'CTG
Cuts at: 2029 2273 2029
Size: 244 5694
SalI G'TCGA_C
Cuts at: 1936 3280 1936
Size: 1344 4594
SbfI CC_TGCA'GG
Cuts at: 3290 3290
Size: 5938
ScaI AGT'ACT
Cuts at: 4186 5898 4186
Size: 1712 4226
SfcI C'TryA_G
Cuts at: 826 1045 2030 2148 2215 3286 4421 5102 5293
Size: 219 985 118 67 1071 1135 681 191
Cuts at: 5293 826
Size: 1471
Fragments arranged by size:
1471 1135 1071 985 681 219 191 118
67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5938
SmaI CCC'GGG
Cuts at: 1872 3265 1872
Size: 1393 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2866 4042 4943 5205 820
Size: 197 926 923 1176 901 262 1553
Fragments arranged by size:
1553 1176 926 923 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3729 493
Size: 3236 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5938
SphI G_CATG'C
Cuts at: 1132 3296 1132
Size: 2164 3774
SspI AAT'ATT
Cuts at: 5 52 3862 5
Size: 47 3810 2081
Fragments arranged by size:
3810 2081 47
StuI AGG'CCT
Cuts at: 1517 2231 2751 2907 1517
Size: 714 520 156 4548
Fragments arranged by size:
4548 714 520 156
StyI C'CwwG_G
Cuts at: 513 1065 2848 3090 3377 513
Size: 552 1783 242 287 3074
Fragments arranged by size:
3074 1783 552 287 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2235 3059 3965 3982 4135 4320 4765
Size: 1255 655 824 906 17 153 185 445
Cuts at: 4765 325
Size: 1498
Fragments arranged by size:
1498 1255 906 824 655 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4184 5896 96
Size: 274 80 33 51 1443 2207 1712 138
Fragments arranged by size:
2207 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5898 5930 5898
Size: 32 5906
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5938
XbaI T'CTAG_A
Cuts at: 3274 3274
Size: 5938
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2102 2234 3000 3185 3226 2102
Size: 132 766 185 41 4814
Fragments arranged by size:
4814 766 185 132 41
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5938
XmaI C'CCGG_G
Cuts at: 1870 3263 1870
Size: 1393 4545
XmnI GAAnn'nnTTC
Cuts at: 4067 4067
Size: 5938
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3746 276
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI