Restriction map for
pFN21AE0713
(Circular) (Six-base) MAPSORT of: pFN21AE0713.seq Check: 3159 from: 1 to: 5602
pFN21AE0713 5602 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2700 2700
Size: 5602
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3412 278
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2945 1937
Size: 1008 4594
Acc65I G'GTAC_C
Cuts at: 2862 2923 2862
Size: 61 5541
AclI AA'CG_TT
Cuts at: 1563 2901 3729 4102 1563
Size: 1338 828 373 3063
Fragments arranged by size:
3063 1338 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2279 2309 3665 5049 5317 1632
Size: 647 30 1356 1384 268 1917
Fragments arranged by size:
1917 1384 1356 647 268 30
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5405
AflIII A'CryG_T
Cuts at: 1203 4501 5478 1203
Size: 3298 977 1327
Fragments arranged by size:
3298 1327 977
AgeI A'CCGG_T
Cuts at: 4433 4433
Size: 5602
AhdI GACnn_n'nnGTC
Cuts at: 4331 4331
Size: 5602
AleI CACnn'nnGTG
Cuts at: 1166 2654 1166
Size: 1488 4114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5602
AlwNI CAG_nnn'CTG
Cuts at: 2092 2134 2338 2533 3345 4917 2092
Size: 42 204 195 812 1572 2777
Fragments arranged by size:
2777 1572 812 204 195 42
ApaLI G'TGCA_C
Cuts at: 3659 4815 3659
Size: 1156 4446
ApoI r'AATT_y
Cuts at: 1493 2911 3137 3289 5398 1493
Size: 1418 226 152 2109 1697
Fragments arranged by size:
2109 1697 1418 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4156 160
Size: 872 3124 1606
Fragments arranged by size:
3124 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
AvaI C'yCGr_G
Cuts at: 1870 1943 2927 1870
Size: 73 984 4545
Fragments arranged by size:
4545 984 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2846 2854 2879 2887 2846
Size: 8 25 8 5561
Fragments arranged by size:
5561 25 8 8
BamHI G'GATC_C
Cuts at: 2046 2932 3299 2046
Size: 886 367 4349
Fragments arranged by size:
4349 886 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2120 2605 2705 2862 2923
Size: 325 217 627 333 485 100 157 61
Cuts at: 2923 4378 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 485 333 325 217 157
100 61
BanII G_rGCy'C
Cuts at: 1391 2112 2202 2921 1391
Size: 721 90 719 4072
Fragments arranged by size:
4072 721 719 90
BbeI G_GCGC'C
Cuts at: 2124 2124
Size: 5602
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4631
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3793 3827 1903
Size: 34 50 34 1772 34 3678
Fragments arranged by size:
3678 1772 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3494 4704 3494
Size: 1210 4392
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5602
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2720 4213 136
Size: 107 122 71 631 605 1048 1493 1525
Fragments arranged by size:
1525 1493 1048 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5597 5597
Size: 5602
BlpI GC'TnA_GC
Cuts at: 3019 3019
Size: 5602
Bme1580I G_kGCm'C
Cuts at: 1790 3663 4819 1790
Size: 1873 1156 2573
Fragments arranged by size:
2573 1873 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5602
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2734 3333 4291
Size: 416 53 83 399 24 1317 599 958
Cuts at: 4291 442
Size: 1753
Fragments arranged by size:
1753 1317 958 599 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2195 2315 2348 4262 1239
Size: 956 120 33 1914 2579
Fragments arranged by size:
2579 1914 956 120 33
Bpu10I CC'TnA_GC
Cuts at: 2079 2079
Size: 5602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3727 4592 4890 3727
Size: 865 298 4439
Fragments arranged by size:
4439 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4265 882
Size: 617 2766 2219
Fragments arranged by size:
2766 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3393 493
Size: 711 2189 2702
Fragments arranged by size:
2702 2189 711
BsaBI GATnn'nnATC
Cuts at: 5596 5596
Size: 5602
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2121 2545 3409 3791
Size: 53 83 186 936 588 424 864 382
Cuts at: 3791 4426 275
Size: 635 1451
Fragments arranged by size:
1451 936 864 635 588 424 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2277 4035 4433 4707 4854 5265 2277
Size: 1758 398 274 147 411 2614
Fragments arranged by size:
2614 1758 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2837 2867 1178
Size: 30 165 30 1434 30 3913
Fragments arranged by size:
3913 1434 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2651 2861 1186
Size: 1465 210 3927
Fragments arranged by size:
3927 1465 210
BseYI C'CCAG_C
Cuts at: 2008 2160 4805 5301 2008
Size: 152 2645 496 2309
Fragments arranged by size:
2645 2309 496 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2672 1163
Size: 573 936 4093
Fragments arranged by size:
4093 936 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3052 3813 4841 1540
Size: 275 186 1051 761 1028 2301
Fragments arranged by size:
2301 1051 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2112 2202 2661 3663 3748 4819 2112
Size: 90 459 1002 85 1071 2895
Fragments arranged by size:
2895 1071 1002 459 90 85
BsmI GAATG_Cn'
Cuts at: 3113 3206 3113
Size: 93 5509
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2202 2661 2921 3663 3748 4819
Size: 399 322 90 459 260 742 85 1071
Cuts at: 4819 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 459 399 322 260 90
85
BspEI T'CCGG_A
Cuts at: 2277 5265 2277
Size: 2988 2614
BspHI T'CATG_A
Cuts at: 2812 3491 5221 2812
Size: 679 1730 3193
Fragments arranged by size:
3193 1730 679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2346 2700 2957 844
Size: 1502 354 257 3489
Fragments arranged by size:
3489 1502 354 257
BsrBI CCG'CTC
Cuts at: 1394 1823 3489 1394
Size: 429 1666 3507
Fragments arranged by size:
3507 1666 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4097 4271 65
Size: 1183 2849 174 1396
Fragments arranged by size:
2849 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4246 4433 1700
Size: 110 2436 187 2869
Fragments arranged by size:
2869 2436 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5602
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5602
BssSI C'ACGA_G
Cuts at: 3662 4674 3662
Size: 1012 4590
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2695 794
Size: 1901 3701
BstBI TT'CG_AA
Cuts at: 4406 4406
Size: 5602
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5602
BstXI CCAn_nnnn'nTGG
Cuts at: 2486 2486
Size: 5602
BstYI r'GATC_y
Cuts at: 1972 2046 2932 3299 3684 3701 5153 5239 5251
Size: 74 886 367 385 17 1452 86 12
Cuts at: 5251 5597 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 886 385 367 346 86 74
17 12
Bsu36I CC'TnA_GG
Cuts at: 2566 2566
Size: 5602
BtgI C'CryG_G
Cuts at: 513 1065 2614 2733 513
Size: 552 1549 119 3382
Fragments arranged by size:
3382 1549 552 119
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4863
Fragments arranged by size:
4863 683 56
BtsI GCAGTG_nn'
Cuts at: 2684 3118 3912 3932 2684
Size: 434 794 20 4354
Fragments arranged by size:
4354 794 434 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5602
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5567
DraI TTT'AAA
Cuts at: 2906 2906
Size: 5602
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4609 809
Size: 507 3293 1802
Fragments arranged by size:
3293 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2667 2764 3049
Size: 54 1425 50 275 18 837 97 285
Cuts at: 3049 3938 5567 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 837 285 275 97 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3049 1537
Size: 275 1237 4090
Fragments arranged by size:
4090 1237 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2651 3532 1353
Size: 1298 881 3423
Fragments arranged by size:
3423 1298 881
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4189 4563 4709 387
Size: 1064 138 2600 374 146 1280
Fragments arranged by size:
2600 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2110 2200 2110
Size: 90 5512
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2279 2309 2315 2348 3665 4262
Size: 393 563 84 30 6 33 1317 597
Cuts at: 4262 5049 5317 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1317 787 597 563 393 268 84
33 30 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2585 2585
Size: 5602
EcoO109I rG'GnC_Cy
Cuts at: 2064 2089 2232 2320 2335 2408 2722 2777 2064
Size: 25 143 88 15 73 314 55 4889
Fragments arranged by size:
4889 314 143 88 73 55 25 15
EcoRI G'AATT_C
Cuts at: 2911 3289 2911
Size: 378 5224
FspI TGC'GCA
Cuts at: 4108 4108
Size: 5602
HaeII r_GCGC'y
Cuts at: 1678 2086 2124 2575 4749 1678
Size: 408 38 451 2174 2531
Fragments arranged by size:
2531 2174 451 408 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2837 2869 4243 4275 4317
Size: 32 135 32 1265 32 1374 32 42
Cuts at: 4317 4349 1373
Size: 32 2626
Fragments arranged by size:
2626 1374 1265 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2946 3192 669
Size: 988 281 1008 246 3079
Fragments arranged by size:
3079 1008 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5602
HpaI GTT'AAC
Cuts at: 3192 3192
Size: 5602
KasI G'GCGC_C
Cuts at: 2120 2120
Size: 5602
KpnI G_GTAC'C
Cuts at: 2866 2927 2866
Size: 61 5541
MfeI C'AATT_G
Cuts at: 3201 3201
Size: 5602
MluI A'CGCG_T
Cuts at: 5478 5478
Size: 5602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4716 4900 1305
Size: 3411 184 2007
Fragments arranged by size:
3411 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2669 2766 10
Size: 54 1425 343 837 97 2846
Fragments arranged by size:
2846 1425 837 343 97 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2654 3560 3919 4078 518
Size: 648 1488 906 359 159 2042
Fragments arranged by size:
2042 1488 906 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3018 3695 4843 5088 1606
Size: 1412 677 1148 245 2120
Fragments arranged by size:
2120 1412 1148 677 245
NarI GG'CG_CC
Cuts at: 2121 2121
Size: 5602
NcoI C'CATG_G
Cuts at: 513 1065 2614 2733 513
Size: 552 1549 119 3382
Fragments arranged by size:
3382 1549 552 119
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5602
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5602
NotI GC'GGCC_GC
Cuts at: 3049 3049
Size: 5602
NspI r_CATG'y
Cuts at: 1132 1229 2638 2960 4505 1132
Size: 97 1409 322 1545 2229
Fragments arranged by size:
2229 1545 1409 322 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5602
PciI A'CATG_T
Cuts at: 4501 4501
Size: 5602
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2173 1108
Size: 1065 4537
PfoI T'CCnGG_A
Cuts at: 2331 2331
Size: 5602
PmeI GTTT'AAAC
Cuts at: 2906 2906
Size: 5602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3703 5184 5216 3671
Size: 32 1481 32 4057
Fragments arranged by size:
4057 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2064 2232 2335 2408 2064
Size: 168 103 73 5258
Fragments arranged by size:
5258 168 103 73
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5602
PsiI TTA'TAA
Cuts at: 3172 3172
Size: 5602
PstI C_TGCA'G
Cuts at: 830 2250 2954 830
Size: 1420 704 3478
Fragments arranged by size:
3478 1420 704
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5602
SacI G_AGCT'C
Cuts at: 2112 2202 2112
Size: 90 5512
SalI G'TCGA_C
Cuts at: 1936 2944 1936
Size: 1008 4594
SanDI GG'GwC_CC
Cuts at: 2064 2064
Size: 5602
SbfI CC_TGCA'GG
Cuts at: 2250 2954 2250
Size: 704 4898
ScaI AGT'ACT
Cuts at: 2036 3850 5562 2036
Size: 1814 1712 2076
Fragments arranged by size:
2076 1814 1712
SfcI C'TryA_G
Cuts at: 826 1045 2246 2950 4085 4766 4957 826
Size: 219 1201 704 1135 681 191 1471
Fragments arranged by size:
1471 1201 1135 704 681 219 191
SfoI GGC'GCC
Cuts at: 2122 2122
Size: 5602
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5602
SmaI CCC'GGG
Cuts at: 1872 2929 1872
Size: 1057 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3706 4607 4869 820
Size: 197 926 1763 901 262 1553
Fragments arranged by size:
1763 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3393 493
Size: 2900 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5602
SphI G_CATG'C
Cuts at: 1132 2960 1132
Size: 1828 3774
SspI AAT'ATT
Cuts at: 5 52 3526 5
Size: 47 3474 2081
Fragments arranged by size:
3474 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5602
StyI C'CwwG_G
Cuts at: 513 1065 2058 2167 2614 2733 3041 513
Size: 552 993 109 447 119 308 3074
Fragments arranged by size:
3074 993 552 447 308 119 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3629 3646 3799 3984 4429 325
Size: 1255 2049 17 153 185 445 1498
Fragments arranged by size:
2049 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2034 3848 5560
Size: 274 80 33 51 1443 57 1814 1712
Cuts at: 5560 96
Size: 138
Fragments arranged by size:
1814 1712 1443 274 138 80 57 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5562 5594 5562
Size: 32 5570
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5602
XbaI T'CTAG_A
Cuts at: 2938 2938
Size: 5602
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2093 2093
Size: 5602
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5602
XmaI C'CCGG_G
Cuts at: 1870 2927 1870
Size: 1057 4545
XmnI GAAnn'nnTTC
Cuts at: 3731 3731
Size: 5602
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3410 276
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacII SapI SexAI SfiI SrfI
SwaI