Restriction map for
pFN21AE0711
(Circular) (Six-base) MAPSORT of: pFN21AE0711.seq Check: 3411 from: 1 to: 5974
pFN21AE0711 5974 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2691 2691
Size: 5974
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2913 3317 1937
Size: 976 404 4594
Fragments arranged by size:
4594 976 404
Acc65I G'GTAC_C
Cuts at: 3295 3295
Size: 5974
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2600 4037 5421 5689 1632
Size: 968 1437 1384 268 1917
Fragments arranged by size:
1917 1437 1384 968 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5777
AflIII A'CryG_T
Cuts at: 1203 4873 5850 1203
Size: 3670 977 1327
Fragments arranged by size:
3670 1327 977
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 4703 4703
Size: 5974
AleI CACnn'nnGTG
Cuts at: 1166 2019 1166
Size: 853 5121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5974
AlwNI CAG_nnn'CTG
Cuts at: 2770 3717 5289 2770
Size: 947 1572 3455
Fragments arranged by size:
3455 1572 947
ApaI G_GGCC'C
Cuts at: 2250 3140 2250
Size: 890 5084
ApaLI G'TGCA_C
Cuts at: 4031 5187 4031
Size: 1156 4818
ApoI r'AATT_y
Cuts at: 1493 3283 3509 3661 5770 1493
Size: 1790 226 152 2109 1697
Fragments arranged by size:
2109 1790 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4528 160
Size: 872 3496 1606
Fragments arranged by size:
3496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 2634 3299 1870
Size: 73 691 665 4545
Fragments arranged by size:
4545 691 665 73
BamHI G'GATC_C
Cuts at: 3304 3671 3304
Size: 367 5607
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2290 2679 2760 3295 4750
Size: 325 217 627 503 389 81 535 1455
Cuts at: 4750 618
Size: 1842
Fragments arranged by size:
1842 1455 627 535 503 389 325 217
81
BanII G_rGCy'C
Cuts at: 1391 2144 2250 2835 3140 3158 3293 1391
Size: 753 106 585 305 18 135 4072
Fragments arranged by size:
4072 753 585 305 135 106 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2086 2539 928
Size: 971 187 453 4363
Fragments arranged by size:
4363 971 453 187
BbvCI CC'TCA_GC
Cuts at: 2717 2717
Size: 5974
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4165 4199 1903
Size: 34 2228 34 3678
Fragments arranged by size:
3678 2228 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3866 5076 3866
Size: 1210 4764
BclI T'GATC_A
Cuts at: 1548 2427 1548
Size: 879 5095
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2288 2639 3135
Size: 107 122 71 631 605 616 351 496
Cuts at: 3135 4585 136
Size: 1450 1525
Fragments arranged by size:
1525 1450 631 616 605 496 351 122
107 71
BglII A'GATC_T
Cuts at: 5969 5969
Size: 5974
BlpI GC'TnA_GC
Cuts at: 2606 3391 2606
Size: 785 5189
Bme1580I G_kGCm'C
Cuts at: 1790 2250 2682 2735 3140 4035 5191 1790
Size: 460 432 53 405 895 1156 2573
Fragments arranged by size:
2573 1156 895 460 432 405 53
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5974
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3134 3705 4663
Size: 416 53 83 399 24 1717 571 958
Cuts at: 4663 442
Size: 1753
Fragments arranged by size:
1753 1717 958 571 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2262 2327 2801 3154 4634 1239
Size: 830 193 65 474 353 1480 2579
Fragments arranged by size:
2579 1480 830 474 353 193 65
Bpu10I CC'TnA_GC
Cuts at: 2058 2138 2690 2717 2058
Size: 80 552 27 5315
Fragments arranged by size:
5315 552 80 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4099 4964 5262 4099
Size: 865 298 4811
Fragments arranged by size:
4811 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2465 3247 4637 882
Size: 617 966 782 1390 2219
Fragments arranged by size:
2219 1390 966 782 617
BsaAI yAC'GTr
Cuts at: 493 1204 2602 3765 493
Size: 711 1398 1163 2702
Fragments arranged by size:
2702 1398 1163 711
BsaBI GATnn'nnATC
Cuts at: 5968 5968
Size: 5974
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3781 4163 4798 275
Size: 53 83 186 936 2248 382 635 1451
Fragments arranged by size:
2248 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2841 4407 4805 5079 5226 5637 2841
Size: 1566 398 274 147 411 3178
Fragments arranged by size:
3178 1566 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3140 3152 3170 3182 1178
Size: 30 165 30 1737 12 18 12 3970
Fragments arranged by size:
3970 1737 165 30 30 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2152 2155 2191 2453 2660 3253 1186
Size: 966 3 36 262 207 593 3907
Fragments arranged by size:
3907 966 593 262 207 36 3
BseYI C'CCAG_C
Cuts at: 2933 3112 5177 5673 2933
Size: 179 2065 496 3234
Fragments arranged by size:
3234 2065 496 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2609 2986 3251 1163
Size: 573 873 377 265 3886
Fragments arranged by size:
3886 873 573 377 265
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3424 4185 5213 1540
Size: 275 186 1423 761 1028 2301
Fragments arranged by size:
2301 1423 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2064 2144 2696 3066 3158 4035 4120 5191 2064
Size: 80 552 370 92 877 85 1071 2847
Fragments arranged by size:
2847 1071 877 552 370 92 85 80
BsmI GAATG_Cn'
Cuts at: 3485 3578 3485
Size: 93 5881
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2144 2250 2682 2696 2735 2835
Size: 399 274 80 106 432 14 39 100
Cuts at: 2835 3066 3140 3158 3293 4035 4120 5191 1391
Size: 231 74 18 135 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 432 399 274 231 135
106 100 85 80 74 39 18 14
BspEI T'CCGG_A
Cuts at: 2841 5637 2841
Size: 2796 3178
BspHI T'CATG_A
Cuts at: 3863 5593 3863
Size: 1730 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2445 2691 2784 3329 844
Size: 1601 246 93 545 3489
Fragments arranged by size:
3489 1601 545 246 93
BsrBI CCG'CTC
Cuts at: 1394 1823 2988 3861 1394
Size: 429 1165 873 3507
Fragments arranged by size:
3507 1165 873 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4469 4643 65
Size: 1183 3221 174 1396
Fragments arranged by size:
3221 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3129 4618 4805 1700
Size: 110 1319 1489 187 2869
Fragments arranged by size:
2869 1489 1319 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5974
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5974
BssSI C'ACGA_G
Cuts at: 4034 5046 4034
Size: 1012 4962
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5974
BstBI TT'CG_AA
Cuts at: 4778 4778
Size: 5974
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5974
BstXI CCAn_nnnn'nTGG
Cuts at: 2905 2905
Size: 5974
BstYI r'GATC_y
Cuts at: 1972 3304 3671 4056 4073 5525 5611 5623 5969
Size: 1332 367 385 17 1452 86 12 346
Cuts at: 5969 1972
Size: 1977
Fragments arranged by size:
1977 1452 1332 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2017 2105 2121 2619 2848 2898 2958
Size: 552 952 88 16 498 229 50 60
Cuts at: 2958 3016 513
Size: 58 3471
Fragments arranged by size:
3471 952 552 498 229 88 60 58
50 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2545 487
Size: 683 56 1319 3916
Fragments arranged by size:
3916 1319 683 56
BtsI GCAGTG_nn'
Cuts at: 3182 3490 4284 4304 3182
Size: 308 794 20 4852
Fragments arranged by size:
4852 794 308 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5939
DraI TTT'AAA
Cuts at: 3278 3278
Size: 5974
DraIII CAC_nnn'GTG
Cuts at: 2602 2602
Size: 5974
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4981 809
Size: 507 3665 1802
Fragments arranged by size:
3665 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2023 2409 2535
Size: 54 1425 50 275 18 193 386 126
Cuts at: 2535 2640 2895 2907 3421 4310 5939 8
Size: 105 255 12 514 889 1629 43
Fragments arranged by size:
1629 1425 889 514 386 275 255 193
126 105 54 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 3421 1537
Size: 275 1609 4090
Fragments arranged by size:
4090 1609 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2269 2351 2516 3017 3904 1353
Size: 916 82 165 501 887 3423
Fragments arranged by size:
3423 916 887 501 165 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2968 3221 4561 4935 5081 387
Size: 1064 138 1379 253 1340 374 146 1280
Fragments arranged by size:
1379 1340 1280 1064 374 253 146 138
EcoICRI GAG'CTC
Cuts at: 2142 3156 2142
Size: 1014 4960
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2262 2327 2600 2801 3154 4037
Size: 393 437 193 65 273 201 353 883
Cuts at: 4037 4634 5421 5689 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 883 787 597 437 393 353 273
268 201 193 65
EcoO109I rG'GnC_Cy
Cuts at: 2235 2246 2247 3136 2235
Size: 11 1 889 5073
Fragments arranged by size:
5073 889 11 1
EcoRI G'AATT_C
Cuts at: 3283 3661 3283
Size: 378 5596
FspI TGC'GCA
Cuts at: 4480 4480
Size: 5974
HaeII r_GCGC'y
Cuts at: 1678 2482 2804 3184 5121 1678
Size: 804 322 380 1937 2531
Fragments arranged by size:
2531 1937 804 380 322
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3140 3172 4615 4647 4689
Size: 32 135 32 1568 32 1443 32 42
Cuts at: 4689 4721 1373
Size: 32 2626
Fragments arranged by size:
2626 1568 1443 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2506 3318 3564 669
Size: 988 281 568 812 246 3079
Fragments arranged by size:
3079 988 812 568 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5974
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KpnI G_GTAC'C
Cuts at: 3299 3299
Size: 5974
MfeI C'AATT_G
Cuts at: 3573 3573
Size: 5974
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2406 5088 5272 1305
Size: 1101 2682 184 2007
Fragments arranged by size:
2682 2007 1101 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2025 2897 10
Size: 54 1425 343 193 872 3087
Fragments arranged by size:
3087 1425 872 343 193 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2019 2888 2903 3932 4291 4450 518
Size: 648 853 869 15 1029 359 159 2042
Fragments arranged by size:
2042 1029 869 853 648 359 159 15
MspA1I CmG'CkG
Cuts at: 1606 2029 2414 2630 2757 3390 4067 5215 5460
Size: 423 385 216 127 633 677 1148 245
Cuts at: 5460 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 633 423 385 245 216
127
NaeI GCC'GGC
Cuts at: 3131 3131
Size: 5974
NcoI C'CATG_G
Cuts at: 513 1065 2105 2121 2898 2958 3016 513
Size: 552 1040 16 777 60 58 3471
Fragments arranged by size:
3471 1040 777 552 60 58 16
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5974
NgoMIV G'CCGG_C
Cuts at: 3129 3129
Size: 5974
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5974
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NspI r_CATG'y
Cuts at: 1132 1229 3332 4877 1132
Size: 97 2103 1545 2229
Fragments arranged by size:
2229 2103 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3268 1348
Size: 1920 4054
PciI A'CATG_T
Cuts at: 4873 4873
Size: 5974
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3177 1108
Size: 2069 3905
PfoI T'CCnGG_A
Cuts at: 3051 3051
Size: 5974
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PmlI CAC'GTG
Cuts at: 2602 2602
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2081 2113 4043 4075 5556 5588 2081
Size: 32 1930 32 1481 32 2467
Fragments arranged by size:
2467 1930 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2235 2235
Size: 5974
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5974
PsiI TTA'TAA
Cuts at: 3544 3544
Size: 5974
PspOMI G'GGCC_C
Cuts at: 2246 3136 2246
Size: 890 5084
PstI C_TGCA'G
Cuts at: 830 2072 3326 830
Size: 1242 1254 3478
Fragments arranged by size:
3478 1254 1242
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
PvuII CAG'CTG
Cuts at: 2029 2630 2029
Size: 601 5373
SacI G_AGCT'C
Cuts at: 2144 3158 2144
Size: 1014 4960
SalI G'TCGA_C
Cuts at: 1936 3316 1936
Size: 1380 4594
SapI GCTCTTCn'nnn_
Cuts at: 3017 3017
Size: 5974
SbfI CC_TGCA'GG
Cuts at: 2072 3326 2072
Size: 1254 4720
ScaI AGT'ACT
Cuts at: 4222 5934 4222
Size: 1712 4262
SfcI C'TryA_G
Cuts at: 826 1045 2068 2722 3322 4457 5138 5329 826
Size: 219 1023 654 600 1135 681 191 1471
Fragments arranged by size:
1471 1135 1023 681 654 600 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3135 3135
Size: 5974
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 2636 3301 1872
Size: 764 665 4545
Fragments arranged by size:
4545 764 665
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4078 4979 5241 820
Size: 197 926 2135 901 262 1553
Fragments arranged by size:
2135 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5974
SphI G_CATG'C
Cuts at: 1132 3332 1132
Size: 2200 3774
SspI AAT'ATT
Cuts at: 5 52 3898 5
Size: 47 3846 2081
Fragments arranged by size:
3846 2081 47
StuI AGG'CCT
Cuts at: 1517 3194 1517
Size: 1677 4297
StyI C'CwwG_G
Cuts at: 513 1065 2105 2121 2898 2958 3016 3413 513
Size: 552 1040 16 777 60 58 397 3074
Fragments arranged by size:
3074 1040 777 552 397 60 58 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2644 4001 4018 4171 4356 4801 325
Size: 1255 1064 1357 17 153 185 445 1498
Fragments arranged by size:
1498 1357 1255 1064 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4220 5932 96
Size: 274 80 33 51 1443 2243 1712 138
Fragments arranged by size:
2243 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2640 2672 2997 3029 5934 5966 2640
Size: 32 325 32 2905 32 2648
Fragments arranged by size:
2905 2648 325 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5974
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2119 2890 2119
Size: 771 5203
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5974
XmaI C'CCGG_G
Cuts at: 1870 2634 3299 1870
Size: 764 665 4545
Fragments arranged by size:
4545 764 665
XmnI GAAnn'nnTTC
Cuts at: 4103 4103
Size: 5974
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PspXI PsrI
RsrII SacII SanDI SexAI SfoI SrfI SwaI