Restriction map for
pFN21AE0707
(Circular) (Six-base) MAPSORT of: pFN21AE0707.seq Check: 8184 from: 1 to: 5983
pFN21AE0707 5983 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2858 2858
Size: 5983
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3793 278
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3326 1937
Size: 1389 4594
Acc65I G'GTAC_C
Cuts at: 3304 3304
Size: 5983
AclI AA'CG_TT
Cuts at: 1563 4110 4483 1563
Size: 2547 373 3063
Fragments arranged by size:
3063 2547 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2287 4046 5430 5698 1632
Size: 655 1759 1384 268 1917
Fragments arranged by size:
1917 1759 1384 655 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5786
AflIII A'CryG_T
Cuts at: 1203 4882 5859 1203
Size: 3679 977 1327
Fragments arranged by size:
3679 1327 977
AgeI A'CCGG_T
Cuts at: 4814 4814
Size: 5983
AhdI GACnn_n'nnGTC
Cuts at: 4712 4712
Size: 5983
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5983
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5983
AlwNI CAG_nnn'CTG
Cuts at: 3726 5298 3726
Size: 1572 4411
ApaI G_GGCC'C
Cuts at: 2166 2533 2639 2683 2938 2166
Size: 367 106 44 255 5211
Fragments arranged by size:
5211 367 255 106 44
ApaLI G'TGCA_C
Cuts at: 2495 3014 4040 5196 2495
Size: 519 1026 1156 3282
Fragments arranged by size:
3282 1156 1026 519
ApoI r'AATT_y
Cuts at: 1493 3292 3518 3670 5779 1493
Size: 1799 226 152 2109 1697
Fragments arranged by size:
2109 1799 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4537 160
Size: 872 3505 1606
Fragments arranged by size:
3505 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
AvaI C'yCGr_G
Cuts at: 1870 1943 3122 3308 1870
Size: 73 1179 186 4545
Fragments arranged by size:
4545 1179 186 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2138 2171 2138
Size: 33 5950
BamHI G'GATC_C
Cuts at: 3313 3680 3313
Size: 367 5616
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3304 4759 618
Size: 325 217 627 1517 1455 1842
Fragments arranged by size:
1842 1517 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2166 2358 2533 2639 2683 2825 2938 3050
Size: 775 192 175 106 44 142 113 112
Cuts at: 3050 3212 3302 1391
Size: 162 90 4072
Fragments arranged by size:
4072 775 192 175 162 142 113 112
106 90 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5012
BbvCI CC'TCA_GC
Cuts at: 3170 3170
Size: 5983
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2852 2886 4174 4208 1903
Size: 34 915 34 1288 34 3678
Fragments arranged by size:
3678 1288 915 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3875 5085 3875
Size: 1210 4773
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5983
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2604 4594 136
Size: 107 122 71 631 605 932 1990 1525
Fragments arranged by size:
1990 1525 932 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5978 5978
Size: 5983
BlpI GC'TnA_GC
Cuts at: 3400 3400
Size: 5983
Bme1580I G_kGCm'C
Cuts at: 1790 2106 2166 2499 2533 2628 2639 2683 2938
Size: 316 60 333 34 95 11 44 255
Cuts at: 2938 3018 4044 5200 1790
Size: 80 1026 1156 2573
Fragments arranged by size:
2573 1156 1026 333 316 255 95 80
60 44 34 11
BmgBI CAC'GTC
Cuts at: 1318 3019 1318
Size: 1701 4282
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2633 3714 4672
Size: 416 53 83 399 24 1216 1081 958
Cuts at: 4672 442
Size: 1753
Fragments arranged by size:
1753 1216 1081 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2061 2372 2489 2843 4643 1239
Size: 822 311 117 354 1800 2579
Fragments arranged by size:
2579 1800 822 354 311 117
Bpu10I CC'TnA_GC
Cuts at: 2468 3170 2468
Size: 702 5281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2135 4108 4973 5271 2135
Size: 1973 865 298 2847
Fragments arranged by size:
2847 1973 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2037 3092 3263 4646 882
Size: 617 538 1055 171 1383 2219
Fragments arranged by size:
2219 1383 1055 617 538 171
BsaAI yAC'GTr
Cuts at: 493 1204 2623 3013 3774 493
Size: 711 1419 390 761 2702
Fragments arranged by size:
2702 1419 761 711 390
BsaBI GATnn'nnATC
Cuts at: 5977 5977
Size: 5983
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3790 4172 4807 275
Size: 53 83 186 936 2257 382 635 1451
Fragments arranged by size:
2257 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4416 4814 5088 5235 5646 4416
Size: 398 274 147 411 4753
Fragments arranged by size:
4753 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3008 3038 3101 3131 1178
Size: 30 165 30 1605 30 63 30 4030
Fragments arranged by size:
4030 1605 165 63 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2001 1186
Size: 815 5168
BseYI C'CCAG_C
Cuts at: 2166 2389 2931 3222 3239 5186 5682 2166
Size: 223 542 291 17 1947 496 2467
Fragments arranged by size:
2467 1947 542 496 291 223 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2585 2603 2720 2978 1163
Size: 573 849 18 117 258 4168
Fragments arranged by size:
4168 849 573 258 117 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2872 2953 3433 4194 5222 1540
Size: 275 186 871 81 480 761 1028 2301
Fragments arranged by size:
2301 1028 871 761 480 275 186 81
BsiHKAI G_wGCw'C
Cuts at: 2358 2454 2499 2754 3018 3069 4044 4129 5200
Size: 96 45 255 264 51 975 85 1071
Cuts at: 5200 2358
Size: 3141
Fragments arranged by size:
3141 1071 975 264 255 96 85 51
45
BsmI GAATG_Cn'
Cuts at: 3494 3587 3494
Size: 93 5890
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2166 2358 2454 2499 2533 2628
Size: 399 316 60 192 96 45 34 95
Cuts at: 2628 2639 2683 2754 2825 2938 3018 3050 3069
Size: 11 44 71 71 113 80 32 19
Cuts at: 3069 3212 3302 4044 4129 5200 1391
Size: 143 90 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 316 192 143 113
96 95 90 85 80 71 71 60
45 44 34 32 19 11
BspEI T'CCGG_A
Cuts at: 5646 5646
Size: 5983
BspHI T'CATG_A
Cuts at: 2742 3872 5602 2742
Size: 1130 1730 3123
Fragments arranged by size:
3123 1730 1130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2214 2331 2766 2858 3338 844
Size: 1370 117 435 92 480 3489
Fragments arranged by size:
3489 1370 480 435 117 92
BsrBI CCG'CTC
Cuts at: 1394 1823 3870 1394
Size: 429 2047 3507
Fragments arranged by size:
3507 2047 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2089 2242 4478 4652 65
Size: 1183 841 153 2236 174 1396
Fragments arranged by size:
2236 1396 1183 841 174 153
BsrFI r'CCGG_y
Cuts at: 1700 1810 4627 4814 1700
Size: 110 2817 187 2869
Fragments arranged by size:
2869 2817 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5983
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5983
BssSI C'ACGA_G
Cuts at: 4043 5055 4043
Size: 1012 4971
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5983
BstBI TT'CG_AA
Cuts at: 4787 4787
Size: 5983
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5983
BstXI CCAn_nnnn'nTGG
Cuts at: 2518 2518
Size: 5983
BstYI r'GATC_y
Cuts at: 1972 3313 3680 4065 4082 5534 5620 5632 5978
Size: 1341 367 385 17 1452 86 12 346
Cuts at: 5978 1972
Size: 1977
Fragments arranged by size:
1977 1452 1341 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2115 2115
Size: 5983
BtgI C'CryG_G
Cuts at: 513 1065 2073 2490 2517 2675 2733 513
Size: 552 1008 417 27 158 58 3763
Fragments arranged by size:
3763 1008 552 417 158 58 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3122 487
Size: 683 56 1896 3348
Fragments arranged by size:
3348 1896 683 56
BtsI GCAGTG_nn'
Cuts at: 2095 2460 3499 4293 4313 2095
Size: 365 1039 794 20 3765
Fragments arranged by size:
3765 1039 794 365 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5983
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5948
DraI TTT'AAA
Cuts at: 3287 3287
Size: 5983
DraIII CAC_nnn'GTG
Cuts at: 3198 3198
Size: 5983
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4990 809
Size: 507 3674 1802
Fragments arranged by size:
3674 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2310 2487
Size: 54 1425 50 275 18 264 216 177
Cuts at: 2487 2736 3054 3430 4319 5948 8
Size: 249 318 376 889 1629 43
Fragments arranged by size:
1629 1425 889 376 318 275 264 249
216 177 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3430 1537
Size: 275 1618 4090
Fragments arranged by size:
4090 1618 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2306 2723 3913 1353
Size: 953 417 1190 3423
Fragments arranged by size:
3423 1190 953 417
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2660 4570 4944 5090 387
Size: 1064 138 1071 1910 374 146 1280
Fragments arranged by size:
1910 1280 1071 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2356 2356
Size: 5983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2061 2287 2372 2489 2843 4046 4643
Size: 393 429 226 85 117 354 1203 597
Cuts at: 4643 5430 5698 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1203 787 597 429 393 354 268
226 117 85
EcoO109I rG'GnC_Cy
Cuts at: 2162 2163 2635 2680 2935 3112 3126 2162
Size: 1 472 45 255 177 14 5019
Fragments arranged by size:
5019 472 255 177 45 14 1
EcoRI G'AATT_C
Cuts at: 3292 3670 3292
Size: 378 5605
FspI TGC'GCA
Cuts at: 2279 4489 2279
Size: 2210 3773
FspAI rTGC'GCAy
Cuts at: 2279 2279
Size: 5983
HaeII r_GCGC'y
Cuts at: 1678 2229 5130 1678
Size: 551 2901 2531
Fragments arranged by size:
2901 2531 551
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3194 3226 4624 4656 4698
Size: 32 135 32 1622 32 1398 32 42
Cuts at: 4698 4730 1373
Size: 32 2626
Fragments arranged by size:
2626 1622 1398 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3327 3573 669
Size: 988 281 1389 246 3079
Fragments arranged by size:
3079 1389 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5983
HpaI GTT'AAC
Cuts at: 3573 3573
Size: 5983
KpnI G_GTAC'C
Cuts at: 3308 3308
Size: 5983
MfeI C'AATT_G
Cuts at: 2023 3582 2023
Size: 1559 4424
MluI A'CGCG_T
Cuts at: 5859 5859
Size: 5983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2298 5097 5281 1305
Size: 993 2799 184 2007
Fragments arranged by size:
2799 2007 993 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2738 10
Size: 54 1425 343 906 3255
Fragments arranged by size:
3255 1425 906 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2696 2747 3941 4300 4459 518
Size: 648 1530 51 1194 359 159 2042
Fragments arranged by size:
2042 1530 1194 648 359 159 51
MspA1I CmG'CkG
Cuts at: 1606 2617 2654 3222 3399 4076 5224 5469 1606
Size: 1011 37 568 177 677 1148 245 2120
Fragments arranged by size:
2120 1148 1011 677 568 245 177 37
NcoI C'CATG_G
Cuts at: 513 1065 2073 2517 2733 513
Size: 552 1008 444 216 3763
Fragments arranged by size:
3763 1008 552 444 216
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5983
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5983
NotI GC'GGCC_GC
Cuts at: 3430 3430
Size: 5983
NspI r_CATG'y
Cuts at: 1132 1229 2665 3341 4886 1132
Size: 97 1436 676 1545 2229
Fragments arranged by size:
2229 1545 1436 676 97
PasI CC'CwG_GG
Cuts at: 1348 2054 3214 1348
Size: 706 1160 4117
Fragments arranged by size:
4117 1160 706
PciI A'CATG_T
Cuts at: 4882 4882
Size: 5983
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3235 1108
Size: 2127 3856
PfoI T'CCnGG_A
Cuts at: 3105 3105
Size: 5983
PmeI GTTT'AAAC
Cuts at: 3287 3287
Size: 5983
PmlI CAC'GTG
Cuts at: 2623 3013 2623
Size: 390 5593
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4052 4084 5565 5597 4052
Size: 32 1481 32 4438
Fragments arranged by size:
4438 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5983
PsiI TTA'TAA
Cuts at: 3553 3553
Size: 5983
PspOMI G'GGCC_C
Cuts at: 2162 2529 2635 2679 2934 2162
Size: 367 106 44 255 5211
Fragments arranged by size:
5211 367 255 106 44
PstI C_TGCA'G
Cuts at: 830 2830 2855 3335 830
Size: 2000 25 480 3478
Fragments arranged by size:
3478 2000 480 25
PvuI CG_AT'CG
Cuts at: 2001 2872 2001
Size: 871 5112
PvuII CAG'CTG
Cuts at: 2617 2654 3222 2617
Size: 37 568 5378
Fragments arranged by size:
5378 568 37
SacI G_AGCT'C
Cuts at: 2358 2358
Size: 5983
SalI G'TCGA_C
Cuts at: 1936 3325 1936
Size: 1389 4594
SapI GCTCTTCn'nnn_
Cuts at: 2723 2723
Size: 5983
SbfI CC_TGCA'GG
Cuts at: 2855 3335 2855
Size: 480 5503
ScaI AGT'ACT
Cuts at: 4231 5943 4231
Size: 1712 4271
SfcI C'TryA_G
Cuts at: 826 1045 2826 2851 3070 3331 4466 5147 5338
Size: 219 1781 25 219 261 1135 681 191
Cuts at: 5338 826
Size: 1471
Fragments arranged by size:
1781 1471 1135 681 261 219 219 191
25
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5983
SmaI CCC'GGG
Cuts at: 1872 3310 1872
Size: 1438 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2150 4087 4988 5250 820
Size: 197 926 207 1937 901 262 1553
Fragments arranged by size:
1937 1553 926 901 262 207 197
SnaBI TAC'GTA
Cuts at: 493 3774 493
Size: 3281 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5983
SphI G_CATG'C
Cuts at: 1132 2665 3341 1132
Size: 1533 676 3774
Fragments arranged by size:
3774 1533 676
SspI AAT'ATT
Cuts at: 5 52 3907 5
Size: 47 3855 2081
Fragments arranged by size:
3855 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5983
StyI C'CwwG_G
Cuts at: 513 1065 2073 2517 2733 2811 3422 513
Size: 552 1008 444 216 78 611 3074
Fragments arranged by size:
3074 1008 611 552 444 216 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2514 3287 4010 4027 4180 4365 4810
Size: 1255 934 773 723 17 153 185 445
Cuts at: 4810 325
Size: 1498
Fragments arranged by size:
1498 1255 934 773 723 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4229 5941 96
Size: 274 80 33 51 1443 2252 1712 138
Fragments arranged by size:
2252 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3008 3040 3101 3133 5943 5975 3008
Size: 32 61 32 2810 32 3016
Fragments arranged by size:
3016 2810 61 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2954 1316
Size: 1638 4345
XbaI T'CTAG_A
Cuts at: 3319 3319
Size: 5983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3236 3236
Size: 5983
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5983
XmaI C'CCGG_G
Cuts at: 1870 3308 1870
Size: 1438 4545
XmnI GAAnn'nnTTC
Cuts at: 4112 4112
Size: 5983
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3791 276
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI
BsmBI BstZ17I EcoNI EcoRV FalI FseI KasI NaeI
NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI