Restriction map for
pFN21AE0704
(Circular) (Six-base) MAPSORT of: pFN21AE0704.seq Check: 7382 from: 1 to: 5926
pFN21AE0704 5926 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3736 278
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3269 1937
Size: 1332 4594
Acc65I G'GTAC_C
Cuts at: 3247 3247
Size: 5926
AclI AA'CG_TT
Cuts at: 1563 4053 4426 1563
Size: 2490 373 3063
Fragments arranged by size:
3063 2490 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2310 2375 2489 2759 2937 3989 5373 5641
Size: 678 65 114 270 178 1052 1384 268
Cuts at: 5641 1632
Size: 1917
Fragments arranged by size:
1917 1384 1052 678 270 268 178 114
65
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5729
AflIII A'CryG_T
Cuts at: 1203 4825 5802 1203
Size: 3622 977 1327
Fragments arranged by size:
3622 1327 977
AgeI A'CCGG_T
Cuts at: 4757 4757
Size: 5926
AhdI GACnn_n'nnGTC
Cuts at: 4655 4655
Size: 5926
AleI CACnn'nnGTG
Cuts at: 1166 3119 1166
Size: 1953 3973
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3086 1184
Size: 1902 4024
AlwNI CAG_nnn'CTG
Cuts at: 2455 3034 3669 5241 2455
Size: 579 635 1572 3140
Fragments arranged by size:
3140 1572 635 579
ApaLI G'TGCA_C
Cuts at: 3983 5139 3983
Size: 1156 4770
ApoI r'AATT_y
Cuts at: 1493 2410 2651 2693 3235 3461 3613 5722 1493
Size: 917 241 42 542 226 152 2109 1697
Fragments arranged by size:
2109 1697 917 542 241 226 152 42
AseI AT'TA_AT
Cuts at: 160 1032 4480 160
Size: 872 3448 1606
Fragments arranged by size:
3448 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 3251 1870
Size: 73 87 1221 4545
Fragments arranged by size:
4545 1221 87 73
BamHI G'GATC_C
Cuts at: 3256 3623 3256
Size: 367 5559
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2098 3247 4702 618
Size: 325 217 627 311 1149 1455 1842
Fragments arranged by size:
1842 1455 1149 627 325 311 217
BanII G_rGCy'C
Cuts at: 1391 3245 1391
Size: 1854 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2859 928
Size: 971 960 3995
Fragments arranged by size:
3995 971 960
BbvCI CC'TCA_GC
Cuts at: 3050 3050
Size: 5926
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4117 4151 1903
Size: 34 50 34 2096 34 3678
Fragments arranged by size:
3678 2096 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2510 3818 5028 2510
Size: 1308 1210 3408
Fragments arranged by size:
3408 1308 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5926
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4537 136
Size: 107 122 71 631 605 2865 1525
Fragments arranged by size:
2865 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2215 5921 2215
Size: 3706 2220
BlpI GC'TnA_GC
Cuts at: 2088 2162 3343 2088
Size: 74 1181 4671
Fragments arranged by size:
4671 1181 74
Bme1580I G_kGCm'C
Cuts at: 1790 3987 5143 1790
Size: 2197 1156 2573
Fragments arranged by size:
2573 2197 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5926
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3044 3103 3657
Size: 416 53 83 399 24 1627 59 554
Cuts at: 3657 4615 442
Size: 958 1753
Fragments arranged by size:
1753 1627 958 554 416 399 83 59
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5926
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2901 2901
Size: 5926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2477 2637 2981 4586 1239
Size: 1238 160 344 1605 2579
Fragments arranged by size:
2579 1605 1238 344 160
Bpu10I CC'TnA_GC
Cuts at: 3050 3050
Size: 5926
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4051 4916 5214 4051
Size: 865 298 4763
Fragments arranged by size:
4763 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4589 882
Size: 617 3090 2219
Fragments arranged by size:
3090 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3066 3717 493
Size: 711 1862 651 2702
Fragments arranged by size:
2702 1862 711 651
BsaBI GATnn'nnATC
Cuts at: 5920 5920
Size: 5926
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2739 3733 4115 4750
Size: 53 83 186 936 1206 994 382 635
Cuts at: 4750 275
Size: 1451
Fragments arranged by size:
1451 1206 994 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4359 4757 5031 5178 5589 4359
Size: 398 274 147 411 4696
Fragments arranged by size:
4696 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5701
Fragments arranged by size:
5701 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2886 3161 1186
Size: 1700 275 3951
Fragments arranged by size:
3951 1700 275
BseYI C'CCAG_C
Cuts at: 2791 3027 5129 5625 2791
Size: 236 2102 496 3092
Fragments arranged by size:
3092 2102 496 236
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2820 1163
Size: 573 1084 4269
Fragments arranged by size:
4269 1084 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3376 4137 5165 1540
Size: 275 186 1375 761 1028 2301
Fragments arranged by size:
2301 1375 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3987 4072 5143 3987
Size: 85 1071 4770
Fragments arranged by size:
4770 1071 85
BsmI GAATG_Cn'
Cuts at: 2731 3437 3530 2731
Size: 706 93 5127
Fragments arranged by size:
5127 706 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3245 3987 4072 5143 1391
Size: 399 1455 742 85 1071 2174
Fragments arranged by size:
2174 1455 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5589 5589
Size: 5926
BspHI T'CATG_A
Cuts at: 3815 5545 3815
Size: 1730 4196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3281 844
Size: 2437 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3813 1394
Size: 429 1990 3507
Fragments arranged by size:
3507 1990 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2152 4421 4595 65
Size: 1183 904 2269 174 1396
Fragments arranged by size:
2269 1396 1183 904 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4570 4757 1700
Size: 110 2760 187 2869
Fragments arranged by size:
2869 2760 187 110
BsrGI T'GTAC_A
Cuts at: 96 2368 96
Size: 2272 3654
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5926
BssSI C'ACGA_G
Cuts at: 3986 4998 3986
Size: 1012 4914
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5926
BstBI TT'CG_AA
Cuts at: 4730 4730
Size: 5926
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5926
BstXI CCAn_nnnn'nTGG
Cuts at: 2591 2591
Size: 5926
BstYI r'GATC_y
Cuts at: 1972 2215 3256 3623 4008 4025 5477 5563 5575
Size: 243 1041 367 385 17 1452 86 12
Cuts at: 5575 5921 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1041 385 367 346 243 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5374
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5187
Fragments arranged by size:
5187 683 56
BtsI GCAGTG_nn'
Cuts at: 3183 3442 4236 4256 3183
Size: 259 794 20 4853
Fragments arranged by size:
4853 794 259 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5926
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5891
DraI TTT'AAA
Cuts at: 3230 3230
Size: 5926
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2738 4933 809
Size: 507 1422 2195 1802
Fragments arranged by size:
2195 1802 1422 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2479 2717 3157
Size: 54 1425 50 275 18 649 238 440
Cuts at: 3157 3373 4262 5891 8
Size: 216 889 1629 43
Fragments arranged by size:
1629 1425 889 649 440 275 238 216
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3373 1537
Size: 275 1561 4090
Fragments arranged by size:
4090 1561 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2778 3856 1353
Size: 1425 1078 3423
Fragments arranged by size:
3423 1425 1078
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4513 4887 5033 387
Size: 1064 138 2924 374 146 1280
Fragments arranged by size:
2924 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2310 2375 2477 2489 2637 2759 2937
Size: 393 678 65 102 12 148 122 178
Cuts at: 2937 2981 3989 4586 5373 5641 1239
Size: 44 1008 597 787 268 1524
Fragments arranged by size:
1524 1008 787 678 597 393 268 178
148 122 102 65 44 12
EcoO109I rG'GnC_Cy
Cuts at: 2401 3017 2401
Size: 616 5310
EcoRI G'AATT_C
Cuts at: 3235 3613 3235
Size: 378 5548
FspI TGC'GCA
Cuts at: 4432 4432
Size: 5926
HaeII r_GCGC'y
Cuts at: 1678 5073 1678
Size: 3395 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2812 2844 2881 2913 2968
Size: 32 135 32 1240 32 37 32 55
Cuts at: 2968 3000 3087 3119 3173 3205 4567 4599 4641
Size: 32 87 32 54 32 1362 32 42
Cuts at: 4641 4673 1373
Size: 32 2626
Fragments arranged by size:
2626 1362 1240 135 87 55 54 42
37 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3270 3516 669
Size: 988 281 1332 246 3079
Fragments arranged by size:
3079 1332 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5926
HpaI GTT'AAC
Cuts at: 3516 3516
Size: 5926
KpnI G_GTAC'C
Cuts at: 3251 3251
Size: 5926
MfeI C'AATT_G
Cuts at: 3525 3525
Size: 5926
MluI A'CGCG_T
Cuts at: 5802 5802
Size: 5926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5040 5224 1305
Size: 3735 184 2007
Fragments arranged by size:
3735 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2481 2719 10
Size: 54 1425 343 649 238 3217
Fragments arranged by size:
3217 1425 649 343 238 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2589 3068 3119 3884 4243 4402 518
Size: 648 1423 479 51 765 359 159 2042
Fragments arranged by size:
2042 1423 765 648 479 359 159 51
MspA1I CmG'CkG
Cuts at: 1606 2161 2364 3156 3342 4019 5167 5412 1606
Size: 555 203 792 186 677 1148 245 2120
Fragments arranged by size:
2120 1148 792 677 555 245 203 186
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5374
NdeI CA'TA_TG
Cuts at: 387 2074 2239 387
Size: 1687 165 4074
Fragments arranged by size:
4074 1687 165
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5926
NotI GC'GGCC_GC
Cuts at: 3373 3373
Size: 5926
NspI r_CATG'y
Cuts at: 1132 1229 3284 4829 1132
Size: 97 2055 1545 2229
Fragments arranged by size:
2229 2055 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5926
PciI A'CATG_T
Cuts at: 4825 4825
Size: 5926
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3034 1108
Size: 1926 4000
PfoI T'CCnGG_A
Cuts at: 2918 2918
Size: 5926
PmeI GTTT'AAAC
Cuts at: 3230 3230
Size: 5926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3995 4027 5508 5540 3995
Size: 32 1481 32 4381
Fragments arranged by size:
4381 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2401 3017 2401
Size: 616 5310
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5926
PsiI TTA'TAA
Cuts at: 3496 3496
Size: 5926
PstI C_TGCA'G
Cuts at: 830 3085 3278 830
Size: 2255 193 3478
Fragments arranged by size:
3478 2255 193
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5926
PvuII CAG'CTG
Cuts at: 2161 2161
Size: 5926
SalI G'TCGA_C
Cuts at: 1936 3268 1936
Size: 1332 4594
SanDI GG'GwC_CC
Cuts at: 3017 3017
Size: 5926
SapI GCTCTTCn'nnn_
Cuts at: 2778 2778
Size: 5926
SbfI CC_TGCA'GG
Cuts at: 3278 3278
Size: 5926
ScaI AGT'ACT
Cuts at: 4174 5886 4174
Size: 1712 4214
SfcI C'TryA_G
Cuts at: 826 1045 3081 3274 4409 5090 5281 826
Size: 219 2036 193 1135 681 191 1471
Fragments arranged by size:
2036 1471 1135 681 219 193 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5926
SmaI CCC'GGG
Cuts at: 1872 2032 3253 1872
Size: 160 1221 4545
Fragments arranged by size:
4545 1221 160
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4030 4931 5193 820
Size: 197 926 2087 901 262 1553
Fragments arranged by size:
2087 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3717 493
Size: 3224 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5926
SphI G_CATG'C
Cuts at: 1132 3284 1132
Size: 2152 3774
SspI AAT'ATT
Cuts at: 5 52 3850 5
Size: 47 3798 2081
Fragments arranged by size:
3798 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5926
StyI C'CwwG_G
Cuts at: 513 1065 2686 2720 3365 513
Size: 552 1621 34 645 3074
Fragments arranged by size:
3074 1621 645 552 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2597 3953 3970 4123 4308 4753 325
Size: 1255 1017 1356 17 153 185 445 1498
Fragments arranged by size:
1498 1356 1255 1017 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2120 2368 3111
Size: 274 80 33 51 1443 143 248 743
Cuts at: 3111 4172 5884 96
Size: 1061 1712 138
Fragments arranged by size:
1712 1443 1061 743 274 248 143 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2682 2714 5886 5918 2682
Size: 32 3172 32 2690
Fragments arranged by size:
3172 2690 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5926
XbaI T'CTAG_A
Cuts at: 3262 3262
Size: 5926
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5926
XmaI C'CCGG_G
Cuts at: 1870 2030 3251 1870
Size: 160 1221 4545
Fragments arranged by size:
4545 1221 160
XmnI GAAnn'nnTTC
Cuts at: 4055 4055
Size: 5926
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3734 276
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI