Restriction map for
pFN21AE0697
(Circular) (Six-base) MAPSORT of: pFN21AE0697.seq Check: 3203 from: 1 to: 7243
pFN21AE0697 7243 bp
With 182 enzymes: SgfI *
March 2, 2010 13:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5053 278
Size: 53 83 186 936 3517 2468
Fragments arranged by size:
3517 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4586 1937
Size: 2649 4594
Acc65I G'GTAC_C
Cuts at: 3548 4564 3548
Size: 1016 6227
AclI AA'CG_TT
Cuts at: 1563 5370 5743 1563
Size: 3807 373 3063
Fragments arranged by size:
3807 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4426 4442 5306 6690 6958 1632
Size: 2794 16 864 1384 268 1917
Fragments arranged by size:
2794 1917 1384 864 268 16
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7046
AflIII A'CryG_T
Cuts at: 1203 3477 6142 7119 1203
Size: 2274 2665 977 1327
Fragments arranged by size:
2665 2274 1327 977
AgeI A'CCGG_T
Cuts at: 6074 6074
Size: 7243
AhdI GACnn_n'nnGTC
Cuts at: 3255 4103 5972 3255
Size: 848 1869 4526
Fragments arranged by size:
4526 1869 848
AleI CACnn'nnGTG
Cuts at: 1166 2492 3143 3992 1166
Size: 1326 651 849 4417
Fragments arranged by size:
4417 1326 849 651
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2783 1184
Size: 1599 5644
AlwNI CAG_nnn'CTG
Cuts at: 2026 2371 2803 3025 3184 4055 4465 4986 6558
Size: 345 432 222 159 871 410 521 1572
Cuts at: 6558 2026
Size: 2711
Fragments arranged by size:
2711 1572 871 521 432 410 345 222
159
ApaI G_GGCC'C
Cuts at: 2278 2912 3506 2278
Size: 634 594 6015
Fragments arranged by size:
6015 634 594
ApaLI G'TGCA_C
Cuts at: 5300 6456 5300
Size: 1156 6087
ApoI r'AATT_y
Cuts at: 1493 2454 4552 4778 4930 7039 1493
Size: 961 2098 226 152 2109 1697
Fragments arranged by size:
2109 2098 1697 961 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5797 160
Size: 872 4765 1606
Fragments arranged by size:
4765 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7243
AvaI C'yCGr_G
Cuts at: 1870 1943 3744 4568 1870
Size: 73 1801 824 4545
Fragments arranged by size:
4545 1801 824 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4244 4277 4244
Size: 33 7210
BamHI G'GATC_C
Cuts at: 2356 4573 4940 2356
Size: 2217 367 4659
Fragments arranged by size:
4659 2217 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2587 2815 2970 3487 3548
Size: 325 217 627 800 228 155 517 61
Cuts at: 3548 3709 4564 6019 618
Size: 161 855 1455 1842
Fragments arranged by size:
1842 1455 855 800 627 517 325 228
217 161 155 61
BanII G_rGCy'C
Cuts at: 1391 2278 2912 3178 3506 3908 4143 4562 1391
Size: 887 634 266 328 402 235 419 4072
Fragments arranged by size:
4072 887 634 419 402 328 266 235
BbeI G_GCGC'C
Cuts at: 2974 2974
Size: 7243
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2140 2241 3467 3823 4301 928
Size: 971 241 101 1226 356 478 3870
Fragments arranged by size:
3870 1226 971 478 356 241 101
BbvCI CC'TCA_GC
Cuts at: 3739 3739
Size: 7243
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3142 3176 5434 5468 1903
Size: 34 1205 34 2258 34 3678
Fragments arranged by size:
3678 2258 1205 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3679 5135 6345 3679
Size: 1456 1210 4577
Fragments arranged by size:
4577 1456 1210
BclI T'GATC_A
Cuts at: 1548 3429 3654 1548
Size: 1881 225 5137
Fragments arranged by size:
5137 1881 225
BfrBI ATG'CAT
Cuts at: 3008 3008
Size: 7243
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2708 4379 4417
Size: 107 122 71 631 605 1036 1671 38
Cuts at: 4417 5854 136
Size: 1437 1525
Fragments arranged by size:
1671 1525 1437 1036 631 605 122 107
71 38
BglII A'GATC_T
Cuts at: 3375 3435 7238 3375
Size: 60 3803 3380
Fragments arranged by size:
3803 3380 60
BlpI GC'TnA_GC
Cuts at: 3177 4208 4660 3177
Size: 1031 452 5760
Fragments arranged by size:
5760 1031 452
Bme1580I G_kGCm'C
Cuts at: 1790 2278 2432 2442 2912 3492 3506 4278 5304
Size: 488 154 10 470 580 14 772 1026
Cuts at: 5304 6460 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1026 772 580 488 470 154
14 10
BmgBI CAC'GTC
Cuts at: 1318 4203 1318
Size: 2885 4358
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3776 4974 5932
Size: 416 53 83 399 24 2359 1198 958
Cuts at: 5932 442
Size: 1753
Fragments arranged by size:
2359 1753 1198 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7243
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3397 3436 3397
Size: 39 7204
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2143 2217 2322 2330 2414 2540 2588
Size: 842 62 74 105 8 84 126 48
Cuts at: 2588 2860 3869 5903 1239
Size: 272 1009 2034 2579
Fragments arranged by size:
2579 2034 1009 842 272 126 105 84
74 62 48 8
Bpu10I CC'TnA_GC
Cuts at: 3739 3885 3739
Size: 146 7097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5368 6233 6531 5368
Size: 865 298 6080
Fragments arranged by size:
6080 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2177 3242 5906 882
Size: 617 678 1065 2664 2219
Fragments arranged by size:
2664 2219 1065 678 617
BsaAI yAC'GTr
Cuts at: 493 1204 5034 493
Size: 711 3830 2702
Fragments arranged by size:
3830 2702 711
BsaBI GATnn'nnATC
Cuts at: 3434 7237 3434
Size: 3803 3440
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2971 5050 5432 6067
Size: 53 83 186 936 1438 2079 382 635
Cuts at: 6067 275
Size: 1451
Fragments arranged by size:
2079 1451 1438 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5676 6074 6348 6495 6906 5676
Size: 398 274 147 411 6013
Fragments arranged by size:
6013 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4445 4475 1178
Size: 30 165 30 3042 30 3946
Fragments arranged by size:
3946 3042 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 2276 2377 3515 3727 4514 1186
Size: 892 198 101 1138 212 787 3915
Fragments arranged by size:
3915 1138 892 787 212 198 101
BseYI C'CCAG_C
Cuts at: 2049 2533 2614 2646 3169 3750 6446 6942 2049
Size: 484 81 32 523 581 2696 496 2350
Fragments arranged by size:
2696 2350 581 523 496 484 81 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2717 2843 3039 3167 1163
Size: 573 981 126 196 128 5239
Fragments arranged by size:
5239 981 573 196 128 126
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4693 5454 6482 1540
Size: 275 186 2692 761 1028 2301
Fragments arranged by size:
2692 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3540 5304 5389 6460 3540
Size: 1764 85 1071 4323
Fragments arranged by size:
4323 1764 1071 85
BsmI GAATG_Cn'
Cuts at: 3008 3153 4754 4847 3008
Size: 145 1601 93 5404
Fragments arranged by size:
5404 1601 145 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2278 2432 2442 2912 3178 3492 3506
Size: 399 488 154 10 470 266 314 14
Cuts at: 3506 3540 3908 4143 4278 4562 5304 5389 6460
Size: 34 368 235 135 284 742 85 1071
Cuts at: 6460 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 488 470 399 368 314
284 266 235 154 135 85 34 14
10
BspEI T'CCGG_A
Cuts at: 6906 6906
Size: 7243
BspHI T'CATG_A
Cuts at: 5132 6862 5132
Size: 1730 5513
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2184 3933 4598 844
Size: 1340 1749 665 3489
Fragments arranged by size:
3489 1749 1340 665
BsrBI CCG'CTC
Cuts at: 1394 1823 4435 5130 1394
Size: 429 2612 695 3507
Fragments arranged by size:
3507 2612 695 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5738 5912 65
Size: 1183 4490 174 1396
Fragments arranged by size:
4490 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2527 2584 3545 4369 5887 6074 1700
Size: 110 717 57 961 824 1518 187 2869
Fragments arranged by size:
2869 1518 961 824 717 187 110 57
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7243
BssHII G'CGCG_C
Cuts at: 1925 2045 1925
Size: 120 7123
BssSI C'ACGA_G
Cuts at: 5303 6315 5303
Size: 1012 6231
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2530 2803 794
Size: 1736 273 5234
Fragments arranged by size:
5234 1736 273
BstBI TT'CG_AA
Cuts at: 2839 6047 2839
Size: 3208 4035
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7243
BstXI CCAn_nnnn'nTGG
Cuts at: 4135 4540 4135
Size: 405 6838
BstYI r'GATC_y
Cuts at: 1972 2241 2356 3375 3435 4065 4573 4940 5325
Size: 269 115 1019 60 630 508 367 385
Cuts at: 5325 5342 6794 6880 6892 7238 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1019 630 508 385 367 346
269 115 86 60 17 12
Bsu36I CC'TnA_GG
Cuts at: 2250 2622 3498 2250
Size: 372 876 5995
Fragments arranged by size:
5995 876 372
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5752
Fragments arranged by size:
5752 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6504
Fragments arranged by size:
6504 683 56
BtsI GCAGTG_nn'
Cuts at: 2801 3138 3150 4350 4759 5553 5573 2801
Size: 337 12 1200 409 794 20 4471
Fragments arranged by size:
4471 1200 794 409 337 20 12
ClaI AT'CG_AT
Cuts at: 1554 2637 1554
Size: 1083 6160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3753 3788 3853 3888 529
Size: 35 3189 35 65 35 3884
Fragments arranged by size:
3884 3189 65 35 35 35
DraI TTT'AAA
Cuts at: 4547 4547
Size: 7243
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3635 6250 809
Size: 507 2319 2615 1802
Fragments arranged by size:
2615 2319 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2202 2653 3543
Size: 54 1425 50 275 18 372 451 890
Cuts at: 3543 3954 4530 4690 5579 7208 8
Size: 411 576 160 889 1629 43
Fragments arranged by size:
1629 1425 890 889 576 451 411 372
275 160 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4690 1537
Size: 275 2878 4090
Fragments arranged by size:
4090 2878 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2411 2948 3164 5173 1353
Size: 1058 537 216 2009 3423
Fragments arranged by size:
3423 2009 1058 537 216
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3799 5830 6204 6350 387
Size: 1064 138 2210 2031 374 146 1280
Fragments arranged by size:
2210 2031 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2143 2217 2322 2330 2414 2540
Size: 393 449 62 74 105 8 84 126
Cuts at: 2540 2588 2860 3869 4426 4442 5306 5903 6690
Size: 48 272 1009 557 16 864 597 787
Cuts at: 6690 6958 1239
Size: 268 1524
Fragments arranged by size:
1524 1009 864 787 597 557 449 393
272 268 126 105 84 74 62 48
16 8
EcoO109I rG'GnC_Cy
Cuts at: 2085 2275 2314 2618 2626 2890 2908 2950 3022
Size: 190 39 304 8 264 18 42 72
Cuts at: 3022 3206 3502 3503 3637 2085
Size: 184 296 1 134 5691
Fragments arranged by size:
5691 304 296 264 190 184 134 72
42 39 18 8 1
EcoRI G'AATT_C
Cuts at: 2454 4552 4930 2454
Size: 2098 378 4767
Fragments arranged by size:
4767 2098 378
EcoRV GAT'ATC
Cuts at: 2965 2965
Size: 7243
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4507 4507
Size: 7243
FspI TGC'GCA
Cuts at: 3575 5749 3575
Size: 2174 5069
HaeII r_GCGC'y
Cuts at: 1678 2848 2974 6390 1678
Size: 1170 126 3416 2531
Fragments arranged by size:
3416 2531 1170 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2233 2265 2774 2806 3241
Size: 32 135 32 661 32 509 32 435
Cuts at: 3241 3273 4089 4121 5884 5916 5958 5990 1373
Size: 32 816 32 1763 32 42 32 2626
Fragments arranged by size:
2626 1763 816 661 509 435 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4587 4833 669
Size: 988 281 2649 246 3079
Fragments arranged by size:
3079 2649 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7243
HpaI GTT'AAC
Cuts at: 4833 4833
Size: 7243
KasI G'GCGC_C
Cuts at: 2970 2970
Size: 7243
KpnI G_GTAC'C
Cuts at: 3552 4568 3552
Size: 1016 6227
MfeI C'AATT_G
Cuts at: 4842 4842
Size: 7243
MluI A'CGCG_T
Cuts at: 7119 7119
Size: 7243
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3102 3756 6357 6541 1305
Size: 1797 654 2601 184 2007
Fragments arranged by size:
2601 2007 1797 654 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2204 4532 10
Size: 54 1425 343 372 2328 2721
Fragments arranged by size:
2721 2328 1425 372 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2492 2672 3143 3794 3992 4028 4133
Size: 648 1326 180 471 651 198 36 105
Cuts at: 4133 4269 4538 5201 5560 5719 518
Size: 136 269 663 359 159 2042
Fragments arranged by size:
2042 1326 663 651 648 471 359 269
198 180 159 136 105 36
MspA1I CmG'CkG
Cuts at: 1606 2561 4087 4212 4414 4659 5336 6484 6729
Size: 955 1526 125 202 245 677 1148 245
Cuts at: 6729 1606
Size: 2120
Fragments arranged by size:
2120 1526 1148 955 677 245 245 202
125
NaeI GCC'GGC
Cuts at: 4371 4371
Size: 7243
NarI GG'CG_CC
Cuts at: 2971 2971
Size: 7243
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5752
Fragments arranged by size:
5752 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7243
NgoMIV G'CCGG_C
Cuts at: 4369 4369
Size: 7243
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7243
NotI GC'GGCC_GC
Cuts at: 4690 4690
Size: 7243
NsiI A_TGCA'T
Cuts at: 3010 3010
Size: 7243
NspI r_CATG'y
Cuts at: 1132 1229 4309 4601 6146 1132
Size: 97 3080 292 1545 2229
Fragments arranged by size:
3080 2229 1545 292 97
PasI CC'CwG_GG
Cuts at: 1348 2317 4049 1348
Size: 969 1732 4542
Fragments arranged by size:
4542 1732 969
PciI A'CATG_T
Cuts at: 6142 6142
Size: 7243
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3025 3766 1108
Size: 1917 741 4585
Fragments arranged by size:
4585 1917 741
PfoI T'CCnGG_A
Cuts at: 2517 2751 3441 3846 4068 2517
Size: 234 690 405 222 5692
Fragments arranged by size:
5692 690 405 234 222
PmeI GTTT'AAAC
Cuts at: 4547 4547
Size: 7243
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2672 2704 5312 5344 6825 6857 2672
Size: 32 2608 32 1481 32 3058
Fragments arranged by size:
3058 2608 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2618 3022 3206 3637 2618
Size: 404 184 431 6224
Fragments arranged by size:
6224 431 404 184
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7243
PsiI TTA'TAA
Cuts at: 3468 4813 3468
Size: 1345 5898
PspOMI G'GGCC_C
Cuts at: 2274 2908 3502 2274
Size: 634 594 6015
Fragments arranged by size:
6015 634 594
PstI C_TGCA'G
Cuts at: 830 2338 2553 2762 2928 3145 4060 4595 830
Size: 1508 215 209 166 217 915 535 3478
Fragments arranged by size:
3478 1508 915 535 217 215 209 166
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7243
SalI G'TCGA_C
Cuts at: 1936 4585 1936
Size: 2649 4594
SanDI GG'GwC_CC
Cuts at: 3637 3637
Size: 7243
SapI GCTCTTCn'nnn_
Cuts at: 2411 2948 2411
Size: 537 6706
SbfI CC_TGCA'GG
Cuts at: 4595 4595
Size: 7243
ScaI AGT'ACT
Cuts at: 5491 7203 5491
Size: 1712 5531
SfcI C'TryA_G
Cuts at: 826 1045 2334 2549 2758 2924 3141 3455 4056
Size: 219 1289 215 209 166 217 314 601
Cuts at: 4056 4591 5726 6407 6598 826
Size: 535 1135 681 191 1471
Fragments arranged by size:
1471 1289 1135 681 601 535 314 219
217 215 209 191 166
SfiI GGCCn_nnn'nGGCC
Cuts at: 4379 4379
Size: 7243
SfoI GGC'GCC
Cuts at: 2972 2972
Size: 7243
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7243
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7243
SmaI CCC'GGG
Cuts at: 1872 3746 4570 1872
Size: 1874 824 4545
Fragments arranged by size:
4545 1874 824
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5347 6248 6510 820
Size: 197 926 3404 901 262 1553
Fragments arranged by size:
3404 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5034 493
Size: 4541 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7243
SphI G_CATG'C
Cuts at: 1132 4309 4601 1132
Size: 3177 292 3774
Fragments arranged by size:
3774 3177 292
SspI AAT'ATT
Cuts at: 5 52 5167 5
Size: 47 5115 2081
Fragments arranged by size:
5115 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7243
StyI C'CwwG_G
Cuts at: 513 1065 2004 2154 4482 4682 513
Size: 552 939 150 2328 200 3074
Fragments arranged by size:
3074 2328 939 552 200 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2041 2271 2660 3465 3710 3777 3935
Size: 1255 461 230 389 805 245 67 158
Cuts at: 3935 4022 4270 4281 4342 5270 5287 5440 5625
Size: 87 248 11 61 928 17 153 185
Cuts at: 5625 6070 325
Size: 445 1498
Fragments arranged by size:
1498 1255 928 805 461 445 389 248
245 230 185 158 153 87 67 61
17 11
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3264 4168 5489
Size: 274 80 33 51 1443 1287 904 1321
Cuts at: 5489 7201 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1321 1287 904 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4445 4477 7203 7235 4445
Size: 32 2726 32 4453
Fragments arranged by size:
4453 2726 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7243
XbaI T'CTAG_A
Cuts at: 3259 4579 3259
Size: 1320 5923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3655 3655
Size: 7243
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7243
XmaI C'CCGG_G
Cuts at: 1870 3744 4568 1870
Size: 1874 824 4545
Fragments arranged by size:
4545 1874 824
XmnI GAAnn'nnTTC
Cuts at: 2466 5372 2466
Size: 2906 4337
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5051 276
Size: 53 83 186 936 3517 2468
Fragments arranged by size:
3517 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BsiWI BsmBI BstZ17I
DraIII EcoICRI EcoNI FseI FspAI NruI PacI PmlI
PspXI PsrI PvuII RsrII SacI SacII SexAI SrfI
SwaI