Restriction map for
pFN21AE0696
(Circular) (Six-base) MAPSORT of: pFN21AE0696.seq Check: 4542 from: 1 to: 5917
pFN21AE0696 5917 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3727 278
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3180 3260 1937
Size: 1243 80 4594
Fragments arranged by size:
4594 1243 80
Acc65I G'GTAC_C
Cuts at: 3238 3238
Size: 5917
AclI AA'CG_TT
Cuts at: 1563 4044 4417 1563
Size: 2481 373 3063
Fragments arranged by size:
3063 2481 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2847 2907 3980 5364 5632 1632
Size: 1215 60 1073 1384 268 1917
Fragments arranged by size:
1917 1384 1215 1073 268 60
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5720
AflIII A'CryG_T
Cuts at: 1203 2167 4816 5793 1203
Size: 964 2649 977 1327
Fragments arranged by size:
2649 1327 977 964
AgeI A'CCGG_T
Cuts at: 4748 4748
Size: 5917
AhdI GACnn_n'nnGTC
Cuts at: 4646 4646
Size: 5917
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5917
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5917
AlwNI CAG_nnn'CTG
Cuts at: 2858 3660 5232 2858
Size: 802 1572 3543
Fragments arranged by size:
3543 1572 802
ApaLI G'TGCA_C
Cuts at: 3974 5130 3974
Size: 1156 4761
ApoI r'AATT_y
Cuts at: 1493 2075 3226 3452 3604 5713 1493
Size: 582 1151 226 152 2109 1697
Fragments arranged by size:
2109 1697 1151 582 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4471 160
Size: 872 3439 1606
Fragments arranged by size:
3439 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
AvaI C'yCGr_G
Cuts at: 1870 1943 3242 1870
Size: 73 1299 4545
Fragments arranged by size:
4545 1299 73
BamHI G'GATC_C
Cuts at: 3247 3614 3247
Size: 367 5550
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2861 3238 4693 618
Size: 325 217 627 1074 377 1455 1842
Fragments arranged by size:
1842 1455 1074 627 377 325 217
BanII G_rGCy'C
Cuts at: 1391 3236 1391
Size: 1845 4072
BbeI G_GCGC'C
Cuts at: 2865 2865
Size: 5917
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2892 928
Size: 971 993 3953
Fragments arranged by size:
3953 993 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3029 3063 4108 4142 1903
Size: 34 50 34 1008 34 1045 34 3678
Fragments arranged by size:
3678 1045 1008 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2095 3809 5019 2095
Size: 1714 1210 2993
Fragments arranged by size:
2993 1714 1210
BclI T'GATC_A
Cuts at: 1548 2290 1548
Size: 742 5175
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4528 136
Size: 107 122 71 631 605 2856 1525
Fragments arranged by size:
2856 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2891 5912 2891
Size: 3021 2896
BlpI GC'TnA_GC
Cuts at: 3334 3334
Size: 5917
Bme1580I G_kGCm'C
Cuts at: 1790 3978 5134 1790
Size: 2188 1156 2573
Fragments arranged by size:
2573 2188 1156
BmgBI CAC'GTC
Cuts at: 1318 3214 1318
Size: 1896 4021
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3648 4606 442
Size: 416 53 83 399 24 2231 958 1753
Fragments arranged by size:
2231 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4577 1239
Size: 3338 2579
Bpu10I CC'TnA_GC
Cuts at: 2222 2222
Size: 5917
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2550 4042 4907 5205 2550
Size: 1492 865 298 3262
Fragments arranged by size:
3262 1492 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3010 4580 882
Size: 617 1511 1570 2219
Fragments arranged by size:
2219 1570 1511 617
BsaAI yAC'GTr
Cuts at: 493 1204 2168 3708 493
Size: 711 964 1540 2702
Fragments arranged by size:
2702 1540 964 711
BsaBI GATnn'nnATC
Cuts at: 5911 5911
Size: 5917
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2862 3724 4106 4741
Size: 53 83 186 936 1329 862 382 635
Cuts at: 4741 275
Size: 1451
Fragments arranged by size:
1451 1329 936 862 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3202 4350 4748 5022 5169 5580 3202
Size: 1148 398 274 147 411 3539
Fragments arranged by size:
3539 1148 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3073 3103 1178
Size: 30 165 30 1670 30 3992
Fragments arranged by size:
3992 1670 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5917
BseYI C'CCAG_C
Cuts at: 5120 5616 5120
Size: 496 5421
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2831 1163
Size: 573 1095 4249
Fragments arranged by size:
4249 1095 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3367 4128 5156 1540
Size: 275 186 1366 761 1028 2301
Fragments arranged by size:
2301 1366 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3978 4063 5134 3978
Size: 85 1071 4761
Fragments arranged by size:
4761 1071 85
BsmI GAATG_Cn'
Cuts at: 2260 3428 3521 2260
Size: 1168 93 4656
Fragments arranged by size:
4656 1168 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2799 2799
Size: 5917
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3236 3978 4063 5134 1391
Size: 399 1446 742 85 1071 2174
Fragments arranged by size:
2174 1446 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5580 5580
Size: 5917
BspHI T'CATG_A
Cuts at: 3806 5536 3806
Size: 1730 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3272 844
Size: 2428 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3804 1394
Size: 429 1981 3507
Fragments arranged by size:
3507 1981 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4412 4586 65
Size: 1183 3164 174 1396
Fragments arranged by size:
3164 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4561 4748 1700
Size: 110 2751 187 2869
Fragments arranged by size:
2869 2751 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5917
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5917
BssSI C'ACGA_G
Cuts at: 3977 4989 3977
Size: 1012 4905
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5917
BstBI TT'CG_AA
Cuts at: 2083 4721 2083
Size: 2638 3279
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5917
BstYI r'GATC_y
Cuts at: 1972 2891 3247 3614 3999 4016 5468 5554 5566
Size: 919 356 367 385 17 1452 86 12
Cuts at: 5566 5912 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 919 385 367 356 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3156 3156
Size: 5917
BtgI C'CryG_G
Cuts at: 513 1065 2395 3018 513
Size: 552 1330 623 3412
Fragments arranged by size:
3412 1330 623 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2223 487
Size: 683 56 997 4181
Fragments arranged by size:
4181 997 683 56
BtsI GCAGTG_nn'
Cuts at: 2619 3433 4227 4247 2619
Size: 814 794 20 4289
Fragments arranged by size:
4289 814 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5917
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5882
DraI TTT'AAA
Cuts at: 3221 3221
Size: 5917
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4924 809
Size: 507 3608 1802
Fragments arranged by size:
3608 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3364 4253 5882
Size: 54 1425 50 275 18 1534 889 1629
Cuts at: 5882 8
Size: 43
Fragments arranged by size:
1629 1534 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3364 1537
Size: 275 1552 4090
Fragments arranged by size:
4090 1552 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2624 2703 2823 3847 1353
Size: 1271 79 120 1024 3423
Fragments arranged by size:
3423 1271 1024 120 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2249 4504 4878 5024 387
Size: 1064 138 660 2255 374 146 1280
Fragments arranged by size:
2255 1280 1064 660 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2847 2907 3980 4577 5364 5632 1239
Size: 393 1215 60 1073 597 787 268 1524
Fragments arranged by size:
1524 1215 1073 787 597 393 268 60
EcoO109I rG'GnC_Cy
Cuts at: 2299 2882 2299
Size: 583 5334
EcoRI G'AATT_C
Cuts at: 3226 3604 3226
Size: 378 5539
FspI TGC'GCA
Cuts at: 4423 4423
Size: 5917
HaeII r_GCGC'y
Cuts at: 1678 2865 5064 1678
Size: 1187 2199 2531
Fragments arranged by size:
2531 2199 1187
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3073 3105 4558 4590 4632
Size: 32 135 32 1501 32 1453 32 42
Cuts at: 4632 4664 1373
Size: 32 2626
Fragments arranged by size:
2626 1501 1453 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3261 3507 669
Size: 988 281 1323 246 3079
Fragments arranged by size:
3079 1323 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2183 748
Size: 1435 4482
HpaI GTT'AAC
Cuts at: 3507 3507
Size: 5917
KasI G'GCGC_C
Cuts at: 2861 2861
Size: 5917
KpnI G_GTAC'C
Cuts at: 3242 3242
Size: 5917
MfeI C'AATT_G
Cuts at: 2645 3516 2645
Size: 871 5046
MluI A'CGCG_T
Cuts at: 5793 5793
Size: 5917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2152 2822 5031 5215 1305
Size: 847 670 2209 184 2007
Fragments arranged by size:
2209 2007 847 670 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4095
Fragments arranged by size:
4095 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2759 3875 4234 4393 518
Size: 648 1593 1116 359 159 2042
Fragments arranged by size:
2042 1593 1116 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2878 3333 4010 5158 5403 1606
Size: 1272 455 677 1148 245 2120
Fragments arranged by size:
2120 1272 1148 677 455 245
NarI GG'CG_CC
Cuts at: 2862 2862
Size: 5917
NcoI C'CATG_G
Cuts at: 513 1065 2395 3018 513
Size: 552 1330 623 3412
Fragments arranged by size:
3412 1330 623 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5917
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5917
NotI GC'GGCC_GC
Cuts at: 3364 3364
Size: 5917
NspI r_CATG'y
Cuts at: 1132 1229 3275 4820 1132
Size: 97 2046 1545 2229
Fragments arranged by size:
2229 2046 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5917
PciI A'CATG_T
Cuts at: 4816 4816
Size: 5917
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5917
PfoI T'CCnGG_A
Cuts at: 2733 2733
Size: 5917
PmeI GTTT'AAAC
Cuts at: 3221 3221
Size: 5917
PmlI CAC'GTG
Cuts at: 2168 2168
Size: 5917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3986 4018 5499 5531 3986
Size: 32 1481 32 4372
Fragments arranged by size:
4372 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2299 2882 2299
Size: 583 5334
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5917
PsiI TTA'TAA
Cuts at: 3487 3487
Size: 5917
PstI C_TGCA'G
Cuts at: 830 3269 830
Size: 2439 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5917
SalI G'TCGA_C
Cuts at: 1936 3259 1936
Size: 1323 4594
SanDI GG'GwC_CC
Cuts at: 2882 2882
Size: 5917
SbfI CC_TGCA'GG
Cuts at: 3269 3269
Size: 5917
ScaI AGT'ACT
Cuts at: 2273 4165 5877 2273
Size: 1892 1712 2313
Fragments arranged by size:
2313 1892 1712
SfcI C'TryA_G
Cuts at: 826 1045 3265 4400 5081 5272 826
Size: 219 2220 1135 681 191 1471
Fragments arranged by size:
2220 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2863 2863
Size: 5917
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5917
SmaI CCC'GGG
Cuts at: 1872 3244 1872
Size: 1372 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2565 4021 4922 5184 820
Size: 197 926 622 1456 901 262 1553
Fragments arranged by size:
1553 1456 926 901 622 262 197
SnaBI TAC'GTA
Cuts at: 493 3708 493
Size: 3215 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5917
SphI G_CATG'C
Cuts at: 1132 3275 1132
Size: 2143 3774
SspI AAT'ATT
Cuts at: 5 52 3841 5
Size: 47 3789 2081
Fragments arranged by size:
3789 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5917
StyI C'CwwG_G
Cuts at: 513 1065 2395 3018 3051 3356 513
Size: 552 1330 623 33 305 3074
Fragments arranged by size:
3074 1330 623 552 305 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3944 3961 4114 4299 4744 325
Size: 1255 2364 17 153 185 445 1498
Fragments arranged by size:
2364 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2271 4163 5875
Size: 274 80 33 51 1443 294 1892 1712
Cuts at: 5875 96
Size: 138
Fragments arranged by size:
1892 1712 1443 294 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5877 5909 5877
Size: 32 5885
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5917
XbaI T'CTAG_A
Cuts at: 2044 3253 2044
Size: 1209 4708
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2402 2011
Size: 391 5526
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5917
XmaI C'CCGG_G
Cuts at: 1870 3242 1870
Size: 1372 4545
XmnI GAAnn'nnTTC
Cuts at: 2273 2697 4046 2273
Size: 424 1349 4144
Fragments arranged by size:
4144 1349 424
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3725 276
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SapI SexAI SfiI SrfI SwaI