Restriction map for
pFN21AE0691
(Circular) (Six-base) MAPSORT of: pFN21AE0691.seq Check: 9123 from: 1 to: 5548
pFN21AE0691 5548 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2659 3358 278
Size: 53 83 186 936 1123 699 2468
Fragments arranged by size:
2468 1123 936 699 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2891 1937
Size: 954 4594
Acc65I G'GTAC_C
Cuts at: 2869 2869
Size: 5548
AclI AA'CG_TT
Cuts at: 1563 3675 4048 1563
Size: 2112 373 3063
Fragments arranged by size:
3063 2112 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3611 4995 5263 1632
Size: 1979 1384 268 1917
Fragments arranged by size:
1979 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5351
AflIII A'CryG_T
Cuts at: 1203 4447 5424 1203
Size: 3244 977 1327
Fragments arranged by size:
3244 1327 977
AgeI A'CCGG_T
Cuts at: 2616 4379 2616
Size: 1763 3785
AhdI GACnn_n'nnGTC
Cuts at: 4277 4277
Size: 5548
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5548
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5548
AlwNI CAG_nnn'CTG
Cuts at: 2093 3291 4863 2093
Size: 1198 1572 2778
Fragments arranged by size:
2778 1572 1198
ApaLI G'TGCA_C
Cuts at: 3605 4761 3605
Size: 1156 4392
ApoI r'AATT_y
Cuts at: 1493 2857 3083 3235 5344 1493
Size: 1364 226 152 2109 1697
Fragments arranged by size:
2109 1697 1364 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4102 160
Size: 872 3070 1606
Fragments arranged by size:
3070 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
AvaI C'yCGr_G
Cuts at: 1870 1943 2025 2033 2398 2873 1870
Size: 73 82 8 365 475 4545
Fragments arranged by size:
4545 475 365 82 73 8
BamHI G'GATC_C
Cuts at: 2878 3245 2878
Size: 367 5181
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2425 2869 4324 618
Size: 325 217 627 230 408 444 1455 1842
Fragments arranged by size:
1842 1455 627 444 408 325 230 217
BanII G_rGCy'C
Cuts at: 1391 2505 2867 1391
Size: 1114 362 4072
Fragments arranged by size:
4072 1114 362
BbeI G_GCGC'C
Cuts at: 2021 2021
Size: 5548
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2258 928
Size: 971 359 4218
Fragments arranged by size:
4218 971 359
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2059 2093 3739 3773 1903
Size: 34 122 34 1646 34 3678
Fragments arranged by size:
3678 1646 122 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2700 3440 4650 2700
Size: 740 1210 3598
Fragments arranged by size:
3598 1210 740
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5548
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 2156 4159
Size: 107 122 71 631 605 343 141 2003
Cuts at: 4159 136
Size: 1525
Fragments arranged by size:
2003 1525 631 605 343 141 122 107
71
BglII A'GATC_T
Cuts at: 5543 5543
Size: 5548
BlpI GC'TnA_GC
Cuts at: 2965 2965
Size: 5548
Bme1580I G_kGCm'C
Cuts at: 1790 2430 2675 3609 4765 1790
Size: 640 245 934 1156 2573
Fragments arranged by size:
2573 1156 934 640 245
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5548
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3279 4237 442
Size: 416 53 83 399 24 1862 958 1753
Fragments arranged by size:
1862 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5548
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 5548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 2300 2363 2489 2504 2813 4208 1239
Size: 986 75 63 126 15 309 1395 2579
Fragments arranged by size:
2579 1395 986 309 126 75 63 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3673 4538 4836 3673
Size: 865 298 4385
Fragments arranged by size:
4385 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2548 4211 882
Size: 617 1049 1663 2219
Fragments arranged by size:
2219 1663 1049 617
BsaAI yAC'GTr
Cuts at: 493 1204 2515 3339 493
Size: 711 1311 824 2702
Fragments arranged by size:
2702 1311 824 711
BsaBI GATnn'nnATC
Cuts at: 5542 5542
Size: 5548
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2018 2656 3355 3737
Size: 53 83 186 936 485 638 699 382
Cuts at: 3737 4372 275
Size: 635 1451
Fragments arranged by size:
1451 936 699 638 635 485 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2616 3981 4379 4653 4800 5211 2616
Size: 1365 398 274 147 411 2953
Fragments arranged by size:
2953 1365 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2099 2129 2273 2303 1178
Size: 30 165 30 696 30 144 30 4423
Fragments arranged by size:
4423 696 165 144 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2615 2744 1186
Size: 1429 129 3990
Fragments arranged by size:
3990 1429 129
BseYI C'CCAG_C
Cuts at: 2082 2299 2788 4751 5247 2082
Size: 217 489 1963 496 2383
Fragments arranged by size:
2383 1963 496 489 217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4975
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2998 3759 4787 1540
Size: 275 186 997 761 1028 2301
Fragments arranged by size:
2301 1028 997 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2505 2700 3609 3694 4765 2505
Size: 195 909 85 1071 3288
Fragments arranged by size:
3288 1071 909 195 85
BsmI GAATG_Cn'
Cuts at: 3059 3152 3059
Size: 93 5455
BsmBI CGTCTCn'nnnn_
Cuts at: 2528 2528
Size: 5548
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2430 2505 2675 2700 2867 3609 3694
Size: 399 640 75 170 25 167 742 85
Cuts at: 3694 4765 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 640 399 170 167 85
75 25
BspEI T'CCGG_A
Cuts at: 5211 5211
Size: 5548
BspHI T'CATG_A
Cuts at: 3437 5167 3437
Size: 1730 3818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2903 844
Size: 2059 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3435 1394
Size: 429 1612 3507
Fragments arranged by size:
3507 1612 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2813 4043 4217 65
Size: 1183 1565 1230 174 1396
Fragments arranged by size:
1565 1396 1230 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2616 4192 4379 1700
Size: 110 806 1576 187 2869
Fragments arranged by size:
2869 1576 806 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5548
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5548
BssSI C'ACGA_G
Cuts at: 3608 4620 3608
Size: 1012 4536
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5548
BstBI TT'CG_AA
Cuts at: 4352 4352
Size: 5548
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5548
BstYI r'GATC_y
Cuts at: 1972 2382 2878 3245 3630 3647 5099 5185 5197
Size: 410 496 367 385 17 1452 86 12
Cuts at: 5197 5543 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 496 410 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2118 513
Size: 552 939 114 3943
Fragments arranged by size:
3943 939 552 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4809
Fragments arranged by size:
4809 683 56
BtsI GCAGTG_nn'
Cuts at: 3064 3858 3878 3064
Size: 794 20 4734
Fragments arranged by size:
4734 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5548
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5513
DraI TTT'AAA
Cuts at: 2852 2852
Size: 5548
DraIII CAC_nnn'GTG
Cuts at: 2133 2133
Size: 5548
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4555 809
Size: 507 3239 1802
Fragments arranged by size:
3239 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2145 2238
Size: 54 1425 50 275 18 177 138 93
Cuts at: 2238 2448 2995 3884 5513 8
Size: 210 547 889 1629 43
Fragments arranged by size:
1629 1425 889 547 275 210 177 138
93 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2995 1537
Size: 275 1183 4090
Fragments arranged by size:
4090 1183 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3478 1353
Size: 2125 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4135 4509 4655 387
Size: 1064 138 2546 374 146 1280
Fragments arranged by size:
2546 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2503 2503
Size: 5548
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 2300 2363 2489 2504 2813 3611
Size: 393 593 75 63 126 15 309 798
Cuts at: 3611 4208 4995 5263 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 798 787 597 593 393 309 268
126 75 63 15
EcoO109I rG'GnC_Cy
Cuts at: 2029 2304 2416 2749 2029
Size: 275 112 333 4828
Fragments arranged by size:
4828 333 275 112
EcoRI G'AATT_C
Cuts at: 2857 3235 2857
Size: 378 5170
FspI TGC'GCA
Cuts at: 2366 4054 2366
Size: 1688 3860
HaeII r_GCGC'y
Cuts at: 1678 2021 4695 1678
Size: 343 2674 2531
Fragments arranged by size:
2674 2531 343
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4189 4221 4263 4295 1373
Size: 32 135 32 2617 32 42 32 2626
Fragments arranged by size:
2626 2617 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2892 3138 669
Size: 988 281 954 246 3079
Fragments arranged by size:
3079 988 954 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5548
HpaI GTT'AAC
Cuts at: 3138 3138
Size: 5548
KasI G'GCGC_C
Cuts at: 2017 2017
Size: 5548
KpnI G_GTAC'C
Cuts at: 2873 2873
Size: 5548
MfeI C'AATT_G
Cuts at: 3147 3147
Size: 5548
MluI A'CGCG_T
Cuts at: 5424 5424
Size: 5548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4662 4846 1305
Size: 3357 184 2007
Fragments arranged by size:
3357 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3726
Fragments arranged by size:
3726 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3506 3865 4024 518
Size: 648 2340 359 159 2042
Fragments arranged by size:
2340 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2090 2258 2278 2964 3641 4789 5034 1606
Size: 484 168 20 686 677 1148 245 2120
Fragments arranged by size:
2120 1148 686 677 484 245 168 20
NarI GG'CG_CC
Cuts at: 2018 2018
Size: 5548
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4057
Fragments arranged by size:
4057 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5548
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5548
NotI GC'GGCC_GC
Cuts at: 2995 2995
Size: 5548
NspI r_CATG'y
Cuts at: 1132 1229 2906 4451 1132
Size: 97 1677 1545 2229
Fragments arranged by size:
2229 1677 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5548
PciI A'CATG_T
Cuts at: 4447 4447
Size: 5548
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5548
PmeI GTTT'AAAC
Cuts at: 2852 2852
Size: 5548
PmlI CAC'GTG
Cuts at: 2515 2515
Size: 5548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3617 3649 5130 5162 3617
Size: 32 1481 32 4003
Fragments arranged by size:
4003 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2029 2304 2029
Size: 275 5273
PshAI GACnn'nnGTC
Cuts at: 1613 2061 2540 1613
Size: 448 479 4621
Fragments arranged by size:
4621 479 448
PsiI TTA'TAA
Cuts at: 3118 3118
Size: 5548
PstI C_TGCA'G
Cuts at: 830 2316 2334 2900 830
Size: 1486 18 566 3478
Fragments arranged by size:
3478 1486 566 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5548
PvuII CAG'CTG
Cuts at: 2090 2258 2278 2090
Size: 168 20 5360
Fragments arranged by size:
5360 168 20
SacI G_AGCT'C
Cuts at: 2505 2505
Size: 5548
SalI G'TCGA_C
Cuts at: 1936 2890 1936
Size: 954 4594
SanDI GG'GwC_CC
Cuts at: 2029 2029
Size: 5548
SbfI CC_TGCA'GG
Cuts at: 2900 2900
Size: 5548
ScaI AGT'ACT
Cuts at: 3796 5508 3796
Size: 1712 3836
SfcI C'TryA_G
Cuts at: 826 1045 2312 2330 2896 4031 4712 4903 826
Size: 219 1267 18 566 1135 681 191 1471
Fragments arranged by size:
1471 1267 1135 681 566 219 191 18
SfoI GGC'GCC
Cuts at: 2019 2019
Size: 5548
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5548
SmaI CCC'GGG
Cuts at: 1872 2027 2400 2875 1872
Size: 155 373 475 4545
Fragments arranged by size:
4545 475 373 155
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3652 4553 4815 820
Size: 197 926 1709 901 262 1553
Fragments arranged by size:
1709 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3339 493
Size: 2846 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5548
SphI G_CATG'C
Cuts at: 1132 2906 1132
Size: 1774 3774
SspI AAT'ATT
Cuts at: 5 52 3472 5
Size: 47 3420 2081
Fragments arranged by size:
3420 2081 47
StuI AGG'CCT
Cuts at: 1517 2159 1517
Size: 642 4906
StyI C'CwwG_G
Cuts at: 513 1065 2004 2160 2421 2660 2987 513
Size: 552 939 156 261 239 327 3074
Fragments arranged by size:
3074 939 552 327 261 239 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3575 3592 3745 3930 4375 325
Size: 1255 1995 17 153 185 445 1498
Fragments arranged by size:
1995 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3794 5506 96
Size: 274 80 33 51 1443 1817 1712 138
Fragments arranged by size:
1817 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2273 2305 5508 5540 2273
Size: 32 3203 32 2281
Fragments arranged by size:
3203 2281 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5548
XbaI T'CTAG_A
Cuts at: 2884 2884
Size: 5548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2158 2158
Size: 5548
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5548
XmaI C'CCGG_G
Cuts at: 1870 2025 2398 2873 1870
Size: 155 373 475 4545
Fragments arranged by size:
4545 475 373 155
XmnI GAAnn'nnTTC
Cuts at: 3677 3677
Size: 5548
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2657 3356 276
Size: 53 83 186 936 1123 699 2468
Fragments arranged by size:
2468 1123 936 699 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PspOMI PspXI PsrI RsrII SacII SapI SexAI
SfiI SrfI SwaI