Restriction map for
pFN21AE0688
(Circular) (Six-base) MAPSORT of: pFN21AE0688.seq Check: 6446 from: 1 to: 5908
pFN21AE0688 5908 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3718 278
Size: 53 83 186 936 2182 2468
Fragments arranged by size:
2468 2182 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3251 1937
Size: 1314 4594
Acc65I G'GTAC_C
Cuts at: 3229 3229
Size: 5908
AclI AA'CG_TT
Cuts at: 1563 4035 4408 1563
Size: 2472 373 3063
Fragments arranged by size:
3063 2472 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2078 2195 2261 3971 5355 5623 1632
Size: 446 117 66 1710 1384 268 1917
Fragments arranged by size:
1917 1710 1384 446 268 117 66
AflII C'TTAA_G
Cuts at: 820 1017 2903 820
Size: 197 1886 3825
Fragments arranged by size:
3825 1886 197
AflIII A'CryG_T
Cuts at: 1203 4807 5784 1203
Size: 3604 977 1327
Fragments arranged by size:
3604 1327 977
AgeI A'CCGG_T
Cuts at: 4739 4739
Size: 5908
AhdI GACnn_n'nnGTC
Cuts at: 4637 4637
Size: 5908
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5908
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5908
AlwNI CAG_nnn'CTG
Cuts at: 2468 2764 2785 3651 5223 2468
Size: 296 21 866 1572 3153
Fragments arranged by size:
3153 1572 866 296 21
ApaLI G'TGCA_C
Cuts at: 3965 5121 3965
Size: 1156 4752
ApoI r'AATT_y
Cuts at: 1493 3033 3077 3176 3217 3443 3595 5704 1493
Size: 1540 44 99 41 226 152 2109 1697
Fragments arranged by size:
2109 1697 1540 226 152 99 44 41
AseI AT'TA_AT
Cuts at: 160 1032 4462 160
Size: 872 3430 1606
Fragments arranged by size:
3430 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
AvaI C'yCGr_G
Cuts at: 1870 1943 2571 3233 1870
Size: 73 628 662 4545
Fragments arranged by size:
4545 662 628 73
AvrII C'CTAG_G
Cuts at: 2023 2023
Size: 5908
BamHI G'GATC_C
Cuts at: 2245 3238 3605 2245
Size: 993 367 4548
Fragments arranged by size:
4548 993 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3041 3229 4684 618
Size: 325 217 627 1254 188 1455 1842
Fragments arranged by size:
1842 1455 1254 627 325 217 188
BanII G_rGCy'C
Cuts at: 1391 3227 1391
Size: 1836 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2607 2816 2970 928
Size: 971 708 209 154 3866
Fragments arranged by size:
3866 971 708 209 154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2818 2852 4099 4133 1903
Size: 34 881 34 1247 34 3678
Fragments arranged by size:
3678 1247 881 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3800 5010 3800
Size: 1210 4698
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5908
BfrBI ATG'CAT
Cuts at: 2864 2864
Size: 5908
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4519 136
Size: 107 122 71 631 605 2847 1525
Fragments arranged by size:
2847 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2666 5903 2666
Size: 3237 2671
BlpI GC'TnA_GC
Cuts at: 3325 3325
Size: 5908
Bme1580I G_kGCm'C
Cuts at: 1790 2714 3046 3969 5125 1790
Size: 924 332 923 1156 2573
Fragments arranged by size:
2573 1156 924 923 332
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5908
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2156 2250 2633
Size: 416 53 83 399 24 739 94 383
Cuts at: 2633 3639 4597 442
Size: 1006 958 1753
Fragments arranged by size:
1753 1006 958 739 416 399 383 94
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5908
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4568 1239
Size: 3329 2579
Bpu10I CC'TnA_GC
Cuts at: 2290 2786 2290
Size: 496 5412
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2581 4033 4898 5196 2581
Size: 1452 865 298 3293
Fragments arranged by size:
3293 1452 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2027 2446 4571 882
Size: 617 528 419 2125 2219
Fragments arranged by size:
2219 2125 617 528 419
BsaAI yAC'GTr
Cuts at: 493 1204 3699 493
Size: 711 2495 2702
Fragments arranged by size:
2702 2495 711
BsaBI GATnn'nnATC
Cuts at: 2883 5902 2883
Size: 3019 2889
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2847 3715 4097 4732
Size: 53 83 186 936 1314 868 382 635
Cuts at: 4732 275
Size: 1451
Fragments arranged by size:
1451 1314 936 868 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2675 4341 4739 5013 5160 5571 2675
Size: 1666 398 274 147 411 3012
Fragments arranged by size:
3012 1666 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5683
Fragments arranged by size:
5683 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2179 2512 1186
Size: 993 333 4582
Fragments arranged by size:
4582 993 333
BseYI C'CCAG_C
Cuts at: 2234 5111 5607 2234
Size: 2877 496 2535
Fragments arranged by size:
2877 2535 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5335
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3358 4119 5147 1540
Size: 275 186 1357 761 1028 2301
Fragments arranged by size:
2301 1357 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2792 3969 4054 5125 2792
Size: 1177 85 1071 3575
Fragments arranged by size:
3575 1177 1071 85
BsmI GAATG_Cn'
Cuts at: 3419 3512 3419
Size: 93 5815
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2714 2792 3046 3227 3969 4054 5125
Size: 399 924 78 254 181 742 85 1071
Cuts at: 5125 1391
Size: 2174
Fragments arranged by size:
2174 1071 924 742 399 254 181 85
78
BspEI T'CCGG_A
Cuts at: 5571 5571
Size: 5908
BspHI T'CATG_A
Cuts at: 3797 5527 3797
Size: 1730 4178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3263 844
Size: 2419 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3795 1394
Size: 429 1972 3507
Fragments arranged by size:
3507 1972 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2526 4403 4577 65
Size: 1183 1278 1877 174 1396
Fragments arranged by size:
1877 1396 1278 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2199 4552 4739 1700
Size: 110 389 2353 187 2869
Fragments arranged by size:
2869 2353 389 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5908
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5908
BssSI C'ACGA_G
Cuts at: 3968 4980 3968
Size: 1012 4896
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5908
BstBI TT'CG_AA
Cuts at: 4712 4712
Size: 5908
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5908
BstXI CCAn_nnnn'nTGG
Cuts at: 2545 2545
Size: 5908
BstYI r'GATC_y
Cuts at: 1972 2245 2458 2666 3238 3605 3990 4007 5459
Size: 273 213 208 572 367 385 17 1452
Cuts at: 5459 5545 5557 5903 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 572 385 367 346 273 213
208 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5356
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5169
Fragments arranged by size:
5169 683 56
BtsI GCAGTG_nn'
Cuts at: 2790 3424 4218 4238 2790
Size: 634 794 20 4460
Fragments arranged by size:
4460 794 634 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5908
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5873
DraI TTT'AAA
Cuts at: 3212 3212
Size: 5908
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4915 809
Size: 507 3599 1802
Fragments arranged by size:
3599 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3355 4244 5873
Size: 54 1425 50 275 18 1525 889 1629
Cuts at: 5873 8
Size: 43
Fragments arranged by size:
1629 1525 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3355 1537
Size: 275 1543 4090
Fragments arranged by size:
4090 1543 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2583 3838 1353
Size: 1230 1255 3423
Fragments arranged by size:
3423 1255 1230
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2532 4495 4869 5015 387
Size: 1064 138 943 1963 374 146 1280
Fragments arranged by size:
1963 1280 1064 943 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2195 2261 3971 4568 5355 5623
Size: 393 446 117 66 1710 597 787 268
Cuts at: 5623 1239
Size: 1524
Fragments arranged by size:
1710 1524 787 597 446 393 268 117
66
EcoO109I rG'GnC_Cy
Cuts at: 2782 2782
Size: 5908
EcoRI G'AATT_C
Cuts at: 3217 3595 3217
Size: 378 5530
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 5908
FspI TGC'GCA
Cuts at: 4414 4414
Size: 5908
HaeII r_GCGC'y
Cuts at: 1678 2347 5055 1678
Size: 669 2708 2531
Fragments arranged by size:
2708 2531 669
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2443 2475 4549 4581 4623
Size: 32 135 32 871 32 2074 32 42
Cuts at: 4623 4655 1373
Size: 32 2626
Fragments arranged by size:
2626 2074 871 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3115 3252 3498 669
Size: 988 281 1177 137 246 3079
Fragments arranged by size:
3079 1177 988 281 246 137
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5908
HpaI GTT'AAC
Cuts at: 3498 3498
Size: 5908
KpnI G_GTAC'C
Cuts at: 3233 3233
Size: 5908
MfeI C'AATT_G
Cuts at: 3507 3507
Size: 5908
MluI A'CGCG_T
Cuts at: 5784 5784
Size: 5908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2696 2992 5022 5206 1305
Size: 1391 296 2030 184 2007
Fragments arranged by size:
2030 2007 1391 296 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4086
Fragments arranged by size:
4086 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3866 4225 4384 518
Size: 648 2700 359 159 2042
Fragments arranged by size:
2700 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2234 2371 2890 3324 4001 5149 5394 1606
Size: 628 137 519 434 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 628 519 434 245 137
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5356
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5908
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5908
NotI GC'GGCC_GC
Cuts at: 3355 3355
Size: 5908
NsiI A_TGCA'T
Cuts at: 2866 2866
Size: 5908
NspI r_CATG'y
Cuts at: 1132 1229 2228 3159 3266 4811 1132
Size: 97 999 931 107 1545 2229
Fragments arranged by size:
2229 1545 999 931 107 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5908
PciI A'CATG_T
Cuts at: 4807 4807
Size: 5908
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2764 1108
Size: 1656 4252
PfoI T'CCnGG_A
Cuts at: 2748 2748
Size: 5908
PmeI GTTT'AAAC
Cuts at: 3212 3212
Size: 5908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3977 4009 5490 5522 3977
Size: 32 1481 32 4363
Fragments arranged by size:
4363 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5908
PsiI TTA'TAA
Cuts at: 3478 3478
Size: 5908
PstI C_TGCA'G
Cuts at: 830 2085 2272 3260 830
Size: 1255 187 988 3478
Fragments arranged by size:
3478 1255 988 187
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5908
PvuII CAG'CTG
Cuts at: 2234 2234
Size: 5908
SalI G'TCGA_C
Cuts at: 1936 3250 1936
Size: 1314 4594
SbfI CC_TGCA'GG
Cuts at: 3260 3260
Size: 5908
ScaI AGT'ACT
Cuts at: 4156 5868 4156
Size: 1712 4196
SfcI C'TryA_G
Cuts at: 826 1045 2081 2268 3256 4391 5072 5263 826
Size: 219 1036 187 988 1135 681 191 1471
Fragments arranged by size:
1471 1135 1036 988 681 219 191 187
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5908
SmaI CCC'GGG
Cuts at: 1872 3235 1872
Size: 1363 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2596 2903 4012 4913 5175 820
Size: 197 926 653 307 1109 901 262 1553
Fragments arranged by size:
1553 1109 926 901 653 307 262 197
SnaBI TAC'GTA
Cuts at: 493 3699 493
Size: 3206 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5908
SphI G_CATG'C
Cuts at: 1132 3266 1132
Size: 2134 3774
SspI AAT'ATT
Cuts at: 5 52 3832 5
Size: 47 3780 2081
Fragments arranged by size:
3780 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5908
StyI C'CwwG_G
Cuts at: 513 1065 2023 2982 3347 513
Size: 552 958 959 365 3074
Fragments arranged by size:
3074 959 958 552 365
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2551 2677 3935 3952 4105 4290 4735
Size: 1255 971 126 1258 17 153 185 445
Cuts at: 4735 325
Size: 1498
Fragments arranged by size:
1498 1258 1255 971 445 185 153 126
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2147 4154 5866
Size: 274 80 33 51 1443 170 2007 1712
Cuts at: 5866 96
Size: 138
Fragments arranged by size:
2007 1712 1443 274 170 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3006 3038 5868 5900 3006
Size: 32 2830 32 3014
Fragments arranged by size:
3014 2830 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5908
XbaI T'CTAG_A
Cuts at: 3244 3244
Size: 5908
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5908
XmaI C'CCGG_G
Cuts at: 1870 3233 1870
Size: 1363 4545
XmnI GAAnn'nnTTC
Cuts at: 4037 4037
Size: 5908
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3716 276
Size: 53 83 186 936 2182 2468
Fragments arranged by size:
2468 2182 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI