Restriction map for
pFN21AE0686
(Circular) (Six-base) MAPSORT of: pFN21AE0686.seq Check: 6378 from: 1 to: 5905
pFN21AE0686 5905 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3715 278
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3248 1937
Size: 1311 4594
Acc65I G'GTAC_C
Cuts at: 3226 3226
Size: 5905
AclI AA'CG_TT
Cuts at: 1563 4032 4405 1563
Size: 2469 373 3063
Fragments arranged by size:
3063 2469 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2273 3050 3176 3968 5352 5620 1632
Size: 641 777 126 792 1384 268 1917
Fragments arranged by size:
1917 1384 792 777 641 268 126
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 2739 4804 5781 1203
Size: 1536 2065 977 1327
Fragments arranged by size:
2065 1536 1327 977
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 2743 4634 2743
Size: 1891 4014
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2255 1184
Size: 1071 4834
AlwNI CAG_nnn'CTG
Cuts at: 2212 2533 3648 5220 2212
Size: 321 1115 1572 2897
Fragments arranged by size:
2897 1572 1115 321
ApaI G_GGCC'C
Cuts at: 3181 3181
Size: 5905
ApaLI G'TGCA_C
Cuts at: 3962 5118 3962
Size: 1156 4749
ApoI r'AATT_y
Cuts at: 1493 2270 2858 3214 3440 3592 5701 1493
Size: 777 588 356 226 152 2109 1697
Fragments arranged by size:
2109 1697 777 588 356 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4459 160
Size: 872 3427 1606
Fragments arranged by size:
3427 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 3174 3230 1870
Size: 73 1231 56 4545
Fragments arranged by size:
4545 1231 73 56
BamHI G'GATC_C
Cuts at: 3235 3602 3235
Size: 367 5538
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2038 3226 4681 618
Size: 325 217 627 251 1188 1455 1842
Fragments arranged by size:
1842 1455 1188 627 325 251 217
BanII G_rGCy'C
Cuts at: 1391 2033 2934 3181 3224 1391
Size: 642 901 247 43 4072
Fragments arranged by size:
4072 901 642 247 43
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2837 928
Size: 971 938 3996
Fragments arranged by size:
3996 971 938
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4096 4130 1903
Size: 34 50 34 2075 34 3678
Fragments arranged by size:
3678 2075 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2793 3797 5007 2793
Size: 1004 1210 3691
Fragments arranged by size:
3691 1210 1004
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5905
BfrBI ATG'CAT
Cuts at: 3137 3137
Size: 5905
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3185 4516 136
Size: 107 122 71 631 605 1513 1331 1525
Fragments arranged by size:
1525 1513 1331 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2244 5900 2244
Size: 3656 2249
BlpI GC'TnA_GC
Cuts at: 3322 3322
Size: 5905
Bme1580I G_kGCm'C
Cuts at: 1790 2348 3181 3966 5122 1790
Size: 558 833 785 1156 2573
Fragments arranged by size:
2573 1156 833 785 558
BmgBI CAC'GTC
Cuts at: 1318 2146 1318
Size: 828 5077
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2335 3636 4594
Size: 416 53 83 399 24 918 1301 958
Cuts at: 4594 442
Size: 1753
Fragments arranged by size:
1753 1301 958 918 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2682 3203 4565 1239
Size: 1443 521 1362 2579
Fragments arranged by size:
2579 1443 1362 521
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4030 4895 5193 4030
Size: 865 298 4742
Fragments arranged by size:
4742 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2460 4568 882
Size: 617 961 2108 2219
Fragments arranged by size:
2219 2108 961 617
BsaAI yAC'GTr
Cuts at: 493 1204 3696 493
Size: 711 2492 2702
Fragments arranged by size:
2702 2492 711
BsaBI GATnn'nnATC
Cuts at: 5899 5899
Size: 5905
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3712 4094 4729 275
Size: 53 83 186 936 2179 382 635 1451
Fragments arranged by size:
2179 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4338 4736 5010 5157 5568 4338
Size: 398 274 147 411 4675
Fragments arranged by size:
4675 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5680
Fragments arranged by size:
5680 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2478 3080 1186
Size: 1292 602 4011
Fragments arranged by size:
4011 1292 602
BseYI C'CCAG_C
Cuts at: 2218 2707 5108 5604 2218
Size: 489 2401 496 2519
Fragments arranged by size:
2519 2401 496 489
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2309 2996 1163
Size: 573 573 687 4072
Fragments arranged by size:
4072 687 573 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3355 4116 5144 1540
Size: 275 186 1354 761 1028 2301
Fragments arranged by size:
2301 1354 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3966 4051 5122 3966
Size: 85 1071 4749
Fragments arranged by size:
4749 1071 85
BsiWI C'GTAC_G
Cuts at: 2356 2356
Size: 5905
BsmI GAATG_Cn'
Cuts at: 2474 2536 3416 3509 2474
Size: 62 880 93 4870
Fragments arranged by size:
4870 880 93 62
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2348 2934 3181 3224 3966 4051
Size: 399 243 315 586 247 43 742 85
Cuts at: 4051 5122 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 586 399 315 247 243
85 43
BspEI T'CCGG_A
Cuts at: 5568 5568
Size: 5905
BspHI T'CATG_A
Cuts at: 2502 3794 5524 2502
Size: 1292 1730 2883
Fragments arranged by size:
2883 1730 1292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3260 844
Size: 2416 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3046 3792 1394
Size: 429 1223 746 3507
Fragments arranged by size:
3507 1223 746 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2547 4400 4574 65
Size: 1183 1299 1853 174 1396
Fragments arranged by size:
1853 1396 1299 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2035 4549 4736 1700
Size: 110 225 2514 187 2869
Fragments arranged by size:
2869 2514 225 187 110
BsrGI T'GTAC_A
Cuts at: 96 2763 96
Size: 2667 3238
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5905
BssSI C'ACGA_G
Cuts at: 3965 4977 3965
Size: 1012 4893
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5905
BstBI TT'CG_AA
Cuts at: 4709 4709
Size: 5905
BstEII G'GTnAC_C
Cuts at: 1184 2197 1184
Size: 1013 4892
BstXI CCAn_nnnn'nTGG
Cuts at: 3082 3082
Size: 5905
BstYI r'GATC_y
Cuts at: 1972 2244 3235 3602 3987 4004 5456 5542 5554
Size: 272 991 367 385 17 1452 86 12
Cuts at: 5554 5900 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 991 385 367 346 272 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2803 513
Size: 552 1738 3615
Fragments arranged by size:
3615 1738 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5166
Fragments arranged by size:
5166 683 56
BtsI GCAGTG_nn'
Cuts at: 3008 3421 4215 4235 3008
Size: 413 794 20 4678
Fragments arranged by size:
4678 794 413 20
ClaI AT'CG_AT
Cuts at: 1554 2184 1554
Size: 630 5275
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5870
DraI TTT'AAA
Cuts at: 3209 3209
Size: 5905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2195 4912 809
Size: 507 879 2717 1802
Fragments arranged by size:
2717 1802 879 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3084 3352 4241
Size: 54 1425 50 275 18 1254 268 889
Cuts at: 4241 5870 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1254 889 275 268 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3352 1537
Size: 275 1540 4090
Fragments arranged by size:
4090 1540 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2501 3152 3835 1353
Size: 1148 651 683 3423
Fragments arranged by size:
3423 1148 683 651
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3178 4492 4866 5012 387
Size: 1064 138 1589 1314 374 146 1280
Fragments arranged by size:
1589 1314 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 2682 3050 3176 3203 3968 4565
Size: 393 641 409 368 126 27 765 597
Cuts at: 4565 5352 5620 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 765 641 597 409 393 368
268 126 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2593 3032 2593
Size: 439 5466
EcoO109I rG'GnC_Cy
Cuts at: 2209 2259 2995 3025 3178 2209
Size: 50 736 30 153 4936
Fragments arranged by size:
4936 736 153 50 30
EcoRI G'AATT_C
Cuts at: 3214 3592 3214
Size: 378 5527
FspI TGC'GCA
Cuts at: 4411 4411
Size: 5905
HaeII r_GCGC'y
Cuts at: 1678 5052 1678
Size: 3374 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2729 2761 4546 4578 4620
Size: 32 135 32 1157 32 1785 32 42
Cuts at: 4620 4652 1373
Size: 32 2626
Fragments arranged by size:
2626 1785 1157 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3100 3249 3495 669
Size: 988 281 1162 149 246 3079
Fragments arranged by size:
3079 1162 988 281 246 149
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5905
HpaI GTT'AAC
Cuts at: 3495 3495
Size: 5905
KpnI G_GTAC'C
Cuts at: 3230 3230
Size: 5905
MfeI C'AATT_G
Cuts at: 3504 3504
Size: 5905
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2037 2823 3165 5019 5203 1305
Size: 732 786 342 1854 184 2007
Fragments arranged by size:
2007 1854 786 732 342 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3086 10
Size: 54 1425 343 1254 2829
Fragments arranged by size:
2829 1425 1254 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2559 2843 3863 4222 4381 518
Size: 648 1393 284 1020 359 159 2042
Fragments arranged by size:
2042 1393 1020 648 359 284 159
MspA1I CmG'CkG
Cuts at: 1606 3321 3998 5146 5391 1606
Size: 1715 677 1148 245 2120
Fragments arranged by size:
2120 1715 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5353
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5905
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5905
NotI GC'GGCC_GC
Cuts at: 3352 3352
Size: 5905
NsiI A_TGCA'T
Cuts at: 3139 3139
Size: 5905
NspI r_CATG'y
Cuts at: 1132 1229 2743 3263 4808 1132
Size: 97 1514 520 1545 2229
Fragments arranged by size:
2229 1545 1514 520 97
PasI CC'CwG_GG
Cuts at: 1348 2020 2749 1348
Size: 672 729 4504
Fragments arranged by size:
4504 729 672
PciI A'CATG_T
Cuts at: 2739 4804 2739
Size: 2065 3840
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2212 1108
Size: 1104 4801
PfoI T'CCnGG_A
Cuts at: 2454 2454
Size: 5905
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 5487 5519 3974
Size: 32 1481 32 4360
Fragments arranged by size:
4360 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2209 2259 2995 3025 2209
Size: 50 736 30 5089
Fragments arranged by size:
5089 736 50 30
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5905
PsiI TTA'TAA
Cuts at: 3475 3475
Size: 5905
PspOMI G'GGCC_C
Cuts at: 3177 3177
Size: 5905
PstI C_TGCA'G
Cuts at: 830 2163 3257 830
Size: 1333 1094 3478
Fragments arranged by size:
3478 1333 1094
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5905
SalI G'TCGA_C
Cuts at: 1936 3247 1936
Size: 1311 4594
SapI GCTCTTCn'nnn_
Cuts at: 2501 2501
Size: 5905
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 4153 5865 4153
Size: 1712 4193
SfcI C'TryA_G
Cuts at: 826 1045 2159 3253 4388 5069 5260 826
Size: 219 1114 1094 1135 681 191 1471
Fragments arranged by size:
1471 1135 1114 1094 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 2035 1700
Size: 335 5570
SmaI CCC'GGG
Cuts at: 1872 3232 1872
Size: 1360 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4009 4910 5172 820
Size: 197 926 2066 901 262 1553
Fragments arranged by size:
2066 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5905
SphI G_CATG'C
Cuts at: 1132 3263 1132
Size: 2131 3774
SspI AAT'ATT
Cuts at: 5 52 3829 5
Size: 47 3777 2081
Fragments arranged by size:
3777 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5905
StyI C'CwwG_G
Cuts at: 513 1065 2301 2637 2972 3081 3344 513
Size: 552 1236 336 335 109 263 3074
Fragments arranged by size:
3074 1236 552 336 335 263 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2741 2951 3932 3949 4102 4287 4732
Size: 1255 1161 210 981 17 153 185 445
Cuts at: 4732 325
Size: 1498
Fragments arranged by size:
1498 1255 1161 981 445 210 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2763 4151 5863
Size: 274 80 33 51 1443 786 1388 1712
Cuts at: 5863 96
Size: 138
Fragments arranged by size:
1712 1443 1388 786 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2135 2167 5865 5897 2135
Size: 32 3698 32 2143
Fragments arranged by size:
3698 2143 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5905
XbaI T'CTAG_A
Cuts at: 3241 3241
Size: 5905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2209 2209
Size: 5905
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5905
XmaI C'CCGG_G
Cuts at: 1870 3230 1870
Size: 1360 4545
XmnI GAAnn'nnTTC
Cuts at: 4034 4034
Size: 5905
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3713 276
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspXI PsrI PvuII RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI