Restriction map for
pFN21AE0684
(Circular) (Six-base) MAPSORT of: pFN21AE0684.seq Check: 5664 from: 1 to: 5902
pFN21AE0684 5902 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2307 3712 278
Size: 53 83 186 936 771 1405 2468
Fragments arranged by size:
2468 1405 936 771 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3245 1937
Size: 1308 4594
Acc65I G'GTAC_C
Cuts at: 3223 3223
Size: 5902
AclI AA'CG_TT
Cuts at: 1563 4029 4402 1563
Size: 2466 373 3063
Fragments arranged by size:
3063 2466 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2064 2455 2840 3965 5349 5617 1632
Size: 432 391 385 1125 1384 268 1917
Fragments arranged by size:
1917 1384 1125 432 391 385 268
AflII C'TTAA_G
Cuts at: 820 1017 2449 820
Size: 197 1432 4273
Fragments arranged by size:
4273 1432 197
AflIII A'CryG_T
Cuts at: 1203 2568 3162 4801 5778 1203
Size: 1365 594 1639 977 1327
Fragments arranged by size:
1639 1365 1327 977 594
AgeI A'CCGG_T
Cuts at: 4733 4733
Size: 5902
AhdI GACnn_n'nnGTC
Cuts at: 4631 4631
Size: 5902
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5902
AlwNI CAG_nnn'CTG
Cuts at: 3645 5217 3645
Size: 1572 4330
ApaLI G'TGCA_C
Cuts at: 3959 5115 3959
Size: 1156 4746
ApoI r'AATT_y
Cuts at: 1493 2231 2256 2378 2716 3211 3437 3589 5698
Size: 738 25 122 338 495 226 152 2109
Cuts at: 5698 1493
Size: 1697
Fragments arranged by size:
2109 1697 738 495 338 226 152 122
25
AseI AT'TA_AT
Cuts at: 160 1032 4456 160
Size: 872 3424 1606
Fragments arranged by size:
3424 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
AvaI C'yCGr_G
Cuts at: 1870 1943 2480 2546 3091 3227 1870
Size: 73 537 66 545 136 4545
Fragments arranged by size:
4545 545 537 136 73 66
BamHI G'GATC_C
Cuts at: 2242 3232 3599 2242
Size: 990 367 4545
Fragments arranged by size:
4545 990 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3223 4678 618
Size: 325 217 627 1436 1455 1842
Fragments arranged by size:
1842 1455 1436 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2916 2991 3098 3221 1391
Size: 1525 75 107 123 4072
Fragments arranged by size:
4072 1525 123 107 75
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2019 2052 2307 2545 2846 928
Size: 971 120 33 255 238 301 3984
Fragments arranged by size:
3984 971 301 255 238 120 33
BbvCI CC'TCA_GC
Cuts at: 2711 2942 2711
Size: 231 5671
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4093 4127 1903
Size: 34 2156 34 3678
Fragments arranged by size:
3678 2156 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3794 5004 3794
Size: 1210 4692
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5902
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4513 136
Size: 107 122 71 631 605 2841 1525
Fragments arranged by size:
2841 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5897 5897
Size: 5902
BlpI GC'TnA_GC
Cuts at: 3319 3319
Size: 5902
Bme1580I G_kGCm'C
Cuts at: 1790 3963 5119 1790
Size: 2173 1156 2573
Fragments arranged by size:
2573 2173 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5902
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2305 3633 4591
Size: 416 53 83 399 24 888 1328 958
Cuts at: 4591 442
Size: 1753
Fragments arranged by size:
1753 1328 958 888 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4562 1239
Size: 3323 2579
Bpu10I CC'TnA_GC
Cuts at: 2711 2942 2711
Size: 231 5671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4027 4892 5190 4027
Size: 865 298 4739
Fragments arranged by size:
4739 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4565 882
Size: 617 3066 2219
Fragments arranged by size:
3066 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2571 2578 3693 493
Size: 711 1367 7 1115 2702
Fragments arranged by size:
2702 1367 1115 711 7
BsaBI GATnn'nnATC
Cuts at: 5896 5896
Size: 5902
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2304 3709 4091 4726
Size: 53 83 186 936 771 1405 382 635
Cuts at: 4726 275
Size: 1451
Fragments arranged by size:
1451 1405 936 771 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3078 4335 4733 5007 5154 5565 3078
Size: 1257 398 274 147 411 3415
Fragments arranged by size:
3415 1257 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2820 2850 1178
Size: 30 165 30 1417 30 4230
Fragments arranged by size:
4230 1417 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2195 2717 3116 3125 1186
Size: 1009 522 399 9 3963
Fragments arranged by size:
3963 1009 522 399 9
BseYI C'CCAG_C
Cuts at: 2824 2999 5105 5601 2824
Size: 175 2106 496 3125
Fragments arranged by size:
3125 2106 496 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5329
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2803 3049 3352 4113 5141 1540
Size: 275 186 802 246 303 761 1028 2301
Fragments arranged by size:
2301 1028 802 761 303 275 246 186
BsiHKAI G_wGCw'C
Cuts at: 2991 3963 4048 5119 2991
Size: 972 85 1071 3774
Fragments arranged by size:
3774 1071 972 85
BsmI GAATG_Cn'
Cuts at: 3413 3506 3413
Size: 93 5809
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2916 2991 3098 3221 3963 4048 5119
Size: 399 1126 75 107 123 742 85 1071
Cuts at: 5119 1391
Size: 2174
Fragments arranged by size:
2174 1126 1071 742 399 123 107 85
75
BspEI T'CCGG_A
Cuts at: 5565 5565
Size: 5902
BspHI T'CATG_A
Cuts at: 3791 5521 3791
Size: 1730 4172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2289 3004 3257 844
Size: 1445 715 253 3489
Fragments arranged by size:
3489 1445 715 253
BsrBI CCG'CTC
Cuts at: 1394 1823 3789 1394
Size: 429 1966 3507
Fragments arranged by size:
3507 1966 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4397 4571 65
Size: 1183 3149 174 1396
Fragments arranged by size:
3149 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2853 4546 4733 1700
Size: 110 1043 1693 187 2869
Fragments arranged by size:
2869 1693 1043 187 110
BsrGI T'GTAC_A
Cuts at: 96 2440 96
Size: 2344 3558
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5902
BssSI C'ACGA_G
Cuts at: 3962 4974 3962
Size: 1012 4890
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5902
BstBI TT'CG_AA
Cuts at: 4706 4706
Size: 5902
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5902
BstYI r'GATC_y
Cuts at: 1972 2242 2751 3081 3148 3232 3599 3984 4001
Size: 270 509 330 67 84 367 385 17
Cuts at: 4001 5453 5539 5551 5897 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 509 385 367 346 330 270
86 84 67 17 12
Bsu36I CC'TnA_GG
Cuts at: 2700 2700
Size: 5902
BtgI C'CryG_G
Cuts at: 513 1065 2004 2217 2643 2671 513
Size: 552 939 213 426 28 3744
Fragments arranged by size:
3744 939 552 426 213 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2644 487
Size: 683 56 1418 3745
Fragments arranged by size:
3745 1418 683 56
BtsI GCAGTG_nn'
Cuts at: 3418 4212 4232 3418
Size: 794 20 5088
Fragments arranged by size:
5088 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5902
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5867
DraI TTT'AAA
Cuts at: 3206 3206
Size: 5902
DraIII CAC_nnn'GTG
Cuts at: 2629 2629
Size: 5902
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4909 809
Size: 507 3593 1802
Fragments arranged by size:
3593 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3046 3349 4238
Size: 54 1425 50 275 18 1216 303 889
Cuts at: 4238 5867 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1216 889 303 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3046 3349 1537
Size: 275 1234 303 4090
Fragments arranged by size:
4090 1234 303 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2055 3832 1353
Size: 702 1777 3423
Fragments arranged by size:
3423 1777 702
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4489 4863 5009 387
Size: 1064 138 2900 374 146 1280
Fragments arranged by size:
2900 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2989 2989
Size: 5902
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2064 2455 2840 3965 4562 5349 5617
Size: 393 432 391 385 1125 597 787 268
Cuts at: 5617 1239
Size: 1524
Fragments arranged by size:
1524 1125 787 597 432 393 391 385
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2699 2699
Size: 5902
EcoO109I rG'GnC_Cy
Cuts at: 2692 2962 2692
Size: 270 5632
EcoRI G'AATT_C
Cuts at: 2256 3211 3589 2256
Size: 955 378 4569
Fragments arranged by size:
4569 955 378
FspI TGC'GCA
Cuts at: 4408 4408
Size: 5902
HaeII r_GCGC'y
Cuts at: 1678 2854 5049 1678
Size: 1176 2195 2531
Fragments arranged by size:
2531 2195 1176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2149 2181 4543 4575 4617
Size: 32 135 32 577 32 2362 32 42
Cuts at: 4617 4649 1373
Size: 32 2626
Fragments arranged by size:
2626 2362 577 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3246 3492 669
Size: 988 281 1308 246 3079
Fragments arranged by size:
3079 1308 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5902
HpaI GTT'AAC
Cuts at: 3492 3492
Size: 5902
KpnI G_GTAC'C
Cuts at: 3227 3227
Size: 5902
MfeI C'AATT_G
Cuts at: 3501 3501
Size: 5902
MluI A'CGCG_T
Cuts at: 5778 5778
Size: 5902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2913 5016 5200 1305
Size: 1608 2103 184 2007
Fragments arranged by size:
2103 2007 1608 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4080
Fragments arranged by size:
4080 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2597 2663 3860 4219 4378 518
Size: 648 1431 66 1197 359 159 2042
Fragments arranged by size:
2042 1431 1197 648 359 159 66
MspA1I CmG'CkG
Cuts at: 1606 2673 3318 3995 5143 5388 1606
Size: 1067 645 677 1148 245 2120
Fragments arranged by size:
2120 1148 1067 677 645 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2217 513
Size: 552 939 213 4198
Fragments arranged by size:
4198 939 552 213
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5902
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5902
NotI GC'GGCC_GC
Cuts at: 3349 3349
Size: 5902
NspI r_CATG'y
Cuts at: 1132 1229 3166 3260 4805 1132
Size: 97 1937 94 1545 2229
Fragments arranged by size:
2229 1937 1545 97 94
PasI CC'CwG_GG
Cuts at: 1348 2916 1348
Size: 1568 4334
PciI A'CATG_T
Cuts at: 3162 4801 3162
Size: 1639 4263
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5902
PfoI T'CCnGG_A
Cuts at: 2754 2754
Size: 5902
PmeI GTTT'AAAC
Cuts at: 3206 3206
Size: 5902
PmlI CAC'GTG
Cuts at: 2571 2571
Size: 5902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 5484 5516 3971
Size: 32 1481 32 4357
Fragments arranged by size:
4357 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2692 2962 2692
Size: 270 5632
PshAI GACnn'nnGTC
Cuts at: 1613 2085 1613
Size: 472 5430
PsiI TTA'TAA
Cuts at: 2322 3472 2322
Size: 1150 4752
PstI C_TGCA'G
Cuts at: 830 3254 830
Size: 2424 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5902
SacI G_AGCT'C
Cuts at: 2991 2991
Size: 5902
SacII CC_GC'GG
Cuts at: 2674 2674
Size: 5902
SalI G'TCGA_C
Cuts at: 1936 3244 1936
Size: 1308 4594
SbfI CC_TGCA'GG
Cuts at: 3254 3254
Size: 5902
ScaI AGT'ACT
Cuts at: 4150 5862 4150
Size: 1712 4190
SfcI C'TryA_G
Cuts at: 826 1045 2285 3250 4385 5066 5257 826
Size: 219 1240 965 1135 681 191 1471
Fragments arranged by size:
1471 1240 1135 965 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
SgrAI Cr'CCGG_yG
Cuts at: 1700 2853 1700
Size: 1153 4749
SmaI CCC'GGG
Cuts at: 1872 2482 2548 3229 1872
Size: 610 66 681 4545
Fragments arranged by size:
4545 681 610 66
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2449 4006 4907 5169 820
Size: 197 926 506 1557 901 262 1553
Fragments arranged by size:
1557 1553 926 901 506 262 197
SnaBI TAC'GTA
Cuts at: 493 3693 493
Size: 3200 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5902
SphI G_CATG'C
Cuts at: 1132 3260 1132
Size: 2128 3774
SspI AAT'ATT
Cuts at: 5 52 2386 3826 5
Size: 47 2334 1440 2081
Fragments arranged by size:
2334 2081 1440 47
StuI AGG'CCT
Cuts at: 1517 2334 3026 1517
Size: 817 692 4393
Fragments arranged by size:
4393 817 692
StyI C'CwwG_G
Cuts at: 513 1065 2004 2217 3341 513
Size: 552 939 213 1124 3074
Fragments arranged by size:
3074 1124 939 552 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2354 2885 3929 3946 4099 4284 4729
Size: 1255 774 531 1044 17 153 185 445
Cuts at: 4729 325
Size: 1498
Fragments arranged by size:
1498 1255 1044 774 531 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2440 4148 5860
Size: 274 80 33 51 1443 463 1708 1712
Cuts at: 5860 96
Size: 138
Fragments arranged by size:
1712 1708 1443 463 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2820 2852 5862 5894 2820
Size: 32 3010 32 2828
Fragments arranged by size:
3010 2828 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5902
XbaI T'CTAG_A
Cuts at: 2077 3238 2077
Size: 1161 4741
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5902
XmaI C'CCGG_G
Cuts at: 1870 2480 2546 3227 1870
Size: 610 66 681 4545
Fragments arranged by size:
4545 681 610 66
XmnI GAAnn'nnTTC
Cuts at: 2073 4031 2073
Size: 1958 3944
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2305 3710 276
Size: 53 83 186 936 771 1405 2468
Fragments arranged by size:
2468 1405 936 771 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspOMI PspXI PsrI PvuII RsrII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI