Restriction map for
pFN21AE0681
(Circular) (Six-base) MAPSORT of: pFN21AE0681.seq Check: 284 from: 1 to: 5515
pFN21AE0681 5515 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3325 278
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2858 1937
Size: 921 4594
Acc65I G'GTAC_C
Cuts at: 2836 2836
Size: 5515
AclI AA'CG_TT
Cuts at: 1563 3642 4015 1563
Size: 2079 373 3063
Fragments arranged by size:
3063 2079 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2246 2281 2354 3578 4962 5230 1632
Size: 614 35 73 1224 1384 268 1917
Fragments arranged by size:
1917 1384 1224 614 268 73 35
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5318
AflIII A'CryG_T
Cuts at: 1203 4414 5391 1203
Size: 3211 977 1327
Fragments arranged by size:
3211 1327 977
AgeI A'CCGG_T
Cuts at: 4346 4346
Size: 5515
AhdI GACnn_n'nnGTC
Cuts at: 4244 4244
Size: 5515
AleI CACnn'nnGTG
Cuts at: 1166 2195 2456 1166
Size: 1029 261 4225
Fragments arranged by size:
4225 1029 261
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5515
AlwNI CAG_nnn'CTG
Cuts at: 2332 3258 4830 2332
Size: 926 1572 3017
Fragments arranged by size:
3017 1572 926
ApaLI G'TGCA_C
Cuts at: 3572 4728 3572
Size: 1156 4359
ApoI r'AATT_y
Cuts at: 1493 2824 3050 3202 5311 1493
Size: 1331 226 152 2109 1697
Fragments arranged by size:
2109 1697 1331 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4069 160
Size: 872 3037 1606
Fragments arranged by size:
3037 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
AvaI C'yCGr_G
Cuts at: 1870 1943 2840 1870
Size: 73 897 4545
Fragments arranged by size:
4545 897 73
BamHI G'GATC_C
Cuts at: 2845 3212 2845
Size: 367 5148
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2836 4291 618
Size: 325 217 627 1049 1455 1842
Fragments arranged by size:
1842 1455 1049 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2834 1391
Size: 1443 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2688 928
Size: 971 789 3755
Fragments arranged by size:
3755 971 789
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2651 2685 3706 3740 1903
Size: 34 714 34 1021 34 3678
Fragments arranged by size:
3678 1021 714 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2052 3407 4617 2052
Size: 1355 1210 2950
Fragments arranged by size:
2950 1355 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5515
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4126 136
Size: 107 122 71 631 605 2454 1525
Fragments arranged by size:
2454 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5510 5510
Size: 5515
BlpI GC'TnA_GC
Cuts at: 2932 2932
Size: 5515
Bme1580I G_kGCm'C
Cuts at: 1790 2674 3576 4732 1790
Size: 884 902 1156 2573
Fragments arranged by size:
2573 1156 902 884
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5515
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2008 3246 4204
Size: 416 53 83 399 24 591 1238 958
Cuts at: 4204 442
Size: 1753
Fragments arranged by size:
1753 1238 958 591 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4175 1239
Size: 2936 2579
Bpu10I CC'TnA_GC
Cuts at: 2319 2319
Size: 5515
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3640 4505 4803 3640
Size: 865 298 4352
Fragments arranged by size:
4352 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2509 2695 4178 882
Size: 617 1010 186 1483 2219
Fragments arranged by size:
2219 1483 1010 617 186
BsaAI yAC'GTr
Cuts at: 493 1204 3306 493
Size: 711 2102 2702
Fragments arranged by size:
2702 2102 711
BsaBI GATnn'nnATC
Cuts at: 5509 5509
Size: 5515
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3322 3704 4339 275
Size: 53 83 186 936 1789 382 635 1451
Fragments arranged by size:
1789 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3948 4346 4620 4767 5178 3948
Size: 398 274 147 411 4285
Fragments arranged by size:
4285 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2299 2329 2675 2705 1178
Size: 30 165 30 896 30 346 30 3988
Fragments arranged by size:
3988 896 346 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5515
BseYI C'CCAG_C
Cuts at: 2788 4718 5214 2788
Size: 1930 496 3089
Fragments arranged by size:
3089 1930 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2744 2780 1163
Size: 573 1008 36 3898
Fragments arranged by size:
3898 1008 573 36
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2965 3726 4754 1540
Size: 275 186 964 761 1028 2301
Fragments arranged by size:
2301 1028 964 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3576 3661 4732 3576
Size: 85 1071 4359
Fragments arranged by size:
4359 1071 85
BsmI GAATG_Cn'
Cuts at: 2626 2787 3026 3119 2626
Size: 161 239 93 5022
Fragments arranged by size:
5022 239 161 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2694 2694
Size: 5515
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2674 2834 3576 3661 4732 1391
Size: 399 884 160 742 85 1071 2174
Fragments arranged by size:
2174 1071 884 742 399 160 85
BspEI T'CCGG_A
Cuts at: 5178 5178
Size: 5515
BspHI T'CATG_A
Cuts at: 3404 5134 3404
Size: 1730 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2870 844
Size: 2026 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3402 1394
Size: 429 1579 3507
Fragments arranged by size:
3507 1579 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4010 4184 65
Size: 1183 2762 174 1396
Fragments arranged by size:
2762 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4159 4346 1700
Size: 110 2349 187 2869
Fragments arranged by size:
2869 2349 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5515
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5515
BssSI C'ACGA_G
Cuts at: 3575 4587 3575
Size: 1012 4503
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5515
BstBI TT'CG_AA
Cuts at: 4319 4319
Size: 5515
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5515
BstXI CCAn_nnnn'nTGG
Cuts at: 2579 2579
Size: 5515
BstYI r'GATC_y
Cuts at: 1972 2845 3212 3597 3614 5066 5152 5164 5510
Size: 873 367 385 17 1452 86 12 346
Cuts at: 5510 1972
Size: 1977
Fragments arranged by size:
1977 1452 873 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2193 513
Size: 552 1128 3835
Fragments arranged by size:
3835 1128 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2481 487
Size: 683 56 1255 3521
Fragments arranged by size:
3521 1255 683 56
BtsI GCAGTG_nn'
Cuts at: 3031 3825 3845 3031
Size: 794 20 4701
Fragments arranged by size:
4701 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5480
DraI TTT'AAA
Cuts at: 2819 2819
Size: 5515
DraIII CAC_nnn'GTG
Cuts at: 2500 2500
Size: 5515
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2602 2714 4522 809
Size: 507 1286 112 1808 1802
Fragments arranged by size:
1808 1802 1286 507 112
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2962 3851 5480
Size: 54 1425 50 275 18 1132 889 1629
Cuts at: 5480 8
Size: 43
Fragments arranged by size:
1629 1425 1132 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2962 1537
Size: 275 1150 4090
Fragments arranged by size:
4090 1150 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2330 3445 1353
Size: 977 1115 3423
Fragments arranged by size:
3423 1115 977
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4102 4476 4622 387
Size: 1064 138 2513 374 146 1280
Fragments arranged by size:
2513 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 2281 2354 3578 4175 4962 5230
Size: 393 614 35 73 1224 597 787 268
Cuts at: 5230 1239
Size: 1524
Fragments arranged by size:
1524 1224 787 614 597 393 268 73
35
EcoO109I rG'GnC_Cy
Cuts at: 2643 2643
Size: 5515
EcoRI G'AATT_C
Cuts at: 2824 3202 2824
Size: 378 5137
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 5515
FspI TGC'GCA
Cuts at: 4021 4021
Size: 5515
HaeII r_GCGC'y
Cuts at: 1678 4662 1678
Size: 2984 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2508 2540 2675 2707 4156
Size: 32 135 32 936 32 135 32 1449
Cuts at: 4156 4188 4230 4262 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1449 936 135 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2059 2719 2859 3105 669
Size: 988 281 121 660 140 246 3079
Fragments arranged by size:
3079 988 660 281 246 140 121
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5515
HpaI GTT'AAC
Cuts at: 3105 3105
Size: 5515
KpnI G_GTAC'C
Cuts at: 2840 2840
Size: 5515
MfeI C'AATT_G
Cuts at: 3114 3114
Size: 5515
MluI A'CGCG_T
Cuts at: 5391 5391
Size: 5515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4629 4813 1305
Size: 3324 184 2007
Fragments arranged by size:
3324 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3693
Fragments arranged by size:
3693 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2195 2456 3473 3832 3991 518
Size: 648 1029 261 1017 359 159 2042
Fragments arranged by size:
2042 1029 1017 648 359 261 159
MspA1I CmG'CkG
Cuts at: 1606 2931 3608 4756 5001 1606
Size: 1325 677 1148 245 2120
Fragments arranged by size:
2120 1325 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4963
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5515
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5515
NotI GC'GGCC_GC
Cuts at: 2962 2962
Size: 5515
NspI r_CATG'y
Cuts at: 1132 1229 2590 2873 4418 1132
Size: 97 1361 283 1545 2229
Fragments arranged by size:
2229 1545 1361 283 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5515
PciI A'CATG_T
Cuts at: 4414 4414
Size: 5515
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5515
PfoI T'CCnGG_A
Cuts at: 2545 2545
Size: 5515
PmeI GTTT'AAAC
Cuts at: 2819 2819
Size: 5515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3584 3616 5097 5129 3584
Size: 32 1481 32 3970
Fragments arranged by size:
3970 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2643 2643
Size: 5515
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5515
PsiI TTA'TAA
Cuts at: 3085 3085
Size: 5515
PstI C_TGCA'G
Cuts at: 830 2867 830
Size: 2037 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5515
SalI G'TCGA_C
Cuts at: 1936 2857 1936
Size: 921 4594
SbfI CC_TGCA'GG
Cuts at: 2867 2867
Size: 5515
ScaI AGT'ACT
Cuts at: 3763 5475 3763
Size: 1712 3803
SfcI C'TryA_G
Cuts at: 826 1045 2324 2863 3998 4679 4870 826
Size: 219 1279 539 1135 681 191 1471
Fragments arranged by size:
1471 1279 1135 681 539 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5515
SmaI CCC'GGG
Cuts at: 1872 2842 1872
Size: 970 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3619 4520 4782 820
Size: 197 926 1676 901 262 1553
Fragments arranged by size:
1676 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3306 493
Size: 2813 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5515
SphI G_CATG'C
Cuts at: 1132 2873 1132
Size: 1741 3774
SspI AAT'ATT
Cuts at: 5 52 3439 5
Size: 47 3387 2081
Fragments arranged by size:
3387 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5515
StyI C'CwwG_G
Cuts at: 513 1065 2572 2954 513
Size: 552 1507 382 3074
Fragments arranged by size:
3074 1507 552 382
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2468 3542 3559 3712 3897 4342 325
Size: 1255 888 1074 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1074 888 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2259 3761 5473
Size: 274 80 33 51 1443 282 1502 1712
Cuts at: 5473 96
Size: 138
Fragments arranged by size:
1712 1502 1443 282 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2022 2054 2299 2331 5475 5507 2022
Size: 32 245 32 3144 32 2030
Fragments arranged by size:
3144 2030 245 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2687 1316
Size: 1371 4144
XbaI T'CTAG_A
Cuts at: 2851 2851
Size: 5515
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5515
XmaI C'CCGG_G
Cuts at: 1870 2840 1870
Size: 970 4545
XmnI GAAnn'nnTTC
Cuts at: 2217 3644 2217
Size: 1427 4088
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3323 276
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI