Restriction map for
pFN21AE0677
(Circular) (Six-base) MAPSORT of: pFN21AE0677.seq Check: 3340 from: 1 to: 5494
pFN21AE0677 5494 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3304 278
Size: 53 83 186 936 1768 2468
Fragments arranged by size:
2468 1768 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2837 1937
Size: 900 4594
Acc65I G'GTAC_C
Cuts at: 2815 2815
Size: 5494
AclI AA'CG_TT
Cuts at: 1563 3621 3994 1563
Size: 2058 373 3063
Fragments arranged by size:
3063 2058 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2114 3557 4941 5209 1632
Size: 482 1443 1384 268 1917
Fragments arranged by size:
1917 1443 1384 482 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5297
AflIII A'CryG_T
Cuts at: 1203 4393 5370 1203
Size: 3190 977 1327
Fragments arranged by size:
3190 1327 977
AgeI A'CCGG_T
Cuts at: 4325 4325
Size: 5494
AhdI GACnn_n'nnGTC
Cuts at: 4223 4223
Size: 5494
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5494
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5494
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2419 2451 2419
Size: 32 5462
AlwNI CAG_nnn'CTG
Cuts at: 2137 2419 2660 3237 4809 2137
Size: 282 241 577 1572 2822
Fragments arranged by size:
2822 1572 577 282 241
ApaI G_GGCC'C
Cuts at: 2537 2537
Size: 5494
ApaLI G'TGCA_C
Cuts at: 3551 4707 3551
Size: 1156 4338
ApoI r'AATT_y
Cuts at: 1493 2803 3029 3181 5290 1493
Size: 1310 226 152 2109 1697
Fragments arranged by size:
2109 1697 1310 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4048 160
Size: 872 3016 1606
Fragments arranged by size:
3016 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5494
AvaI C'yCGr_G
Cuts at: 1870 1943 2147 2819 1870
Size: 73 204 672 4545
Fragments arranged by size:
4545 672 204 73
BamHI G'GATC_C
Cuts at: 2824 3191 2824
Size: 367 5127
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2725 2815 4270 618
Size: 325 217 627 938 90 1455 1842
Fragments arranged by size:
1842 1455 938 627 325 217 90
BanII G_rGCy'C
Cuts at: 1391 2202 2319 2537 2813 1391
Size: 811 117 218 276 4072
Fragments arranged by size:
4072 811 276 218 117
BbeI G_GCGC'C
Cuts at: 2729 2729
Size: 5494
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2176 2279 928
Size: 971 277 103 4143
Fragments arranged by size:
4143 971 277 103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3685 3719 1903
Size: 34 1748 34 3678
Fragments arranged by size:
3678 1748 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3386 4596 3386
Size: 1210 4284
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5494
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2621 2723 4105
Size: 107 122 71 631 605 949 102 1382
Cuts at: 4105 136
Size: 1525
Fragments arranged by size:
1525 1382 949 631 605 122 107 102
71
BglII A'GATC_T
Cuts at: 5489 5489
Size: 5494
BlpI GC'TnA_GC
Cuts at: 2911 2911
Size: 5494
Bme1580I G_kGCm'C
Cuts at: 1790 2042 2537 3555 4711 1790
Size: 252 495 1018 1156 2573
Fragments arranged by size:
2573 1156 1018 495 252
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5494
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3225 4183 442
Size: 416 53 83 399 24 1808 958 1753
Fragments arranged by size:
1808 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5494
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2216 2216
Size: 5494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2325 2423 4154 1239
Size: 1086 98 1731 2579
Fragments arranged by size:
2579 1731 1086 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3619 4484 4782 3619
Size: 865 298 4331
Fragments arranged by size:
4331 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4157 882
Size: 617 2658 2219
Fragments arranged by size:
2658 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3285 493
Size: 711 2081 2702
Fragments arranged by size:
2702 2081 711
BsaBI GATnn'nnATC
Cuts at: 5488 5488
Size: 5494
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2726 3301 3683 4318
Size: 53 83 186 936 1193 575 382 635
Cuts at: 4318 275
Size: 1451
Fragments arranged by size:
1451 1193 936 635 575 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2112 3927 4325 4599 4746 5157 2112
Size: 1815 398 274 147 411 2449
Fragments arranged by size:
2449 1815 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2323 2342 2353 2372 1178
Size: 30 165 30 920 19 11 19 4300
Fragments arranged by size:
4300 920 165 30 30 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2328 1186
Size: 1142 4352
BseYI C'CCAG_C
Cuts at: 2409 4697 5193 2409
Size: 2288 496 2710
Fragments arranged by size:
2710 2288 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2255 1163
Size: 573 519 4402
Fragments arranged by size:
4402 573 519
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2944 3705 4733 1540
Size: 275 186 943 761 1028 2301
Fragments arranged by size:
2301 1028 943 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2202 2546 2652 3555 3640 4711 2202
Size: 344 106 903 85 1071 2985
Fragments arranged by size:
2985 1071 903 344 106 85
BsmI GAATG_Cn'
Cuts at: 3005 3098 3005
Size: 93 5401
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2202 2319 2537 2546 2652 2813
Size: 399 252 160 117 218 9 106 161
Cuts at: 2813 3555 3640 4711 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 252 218 161 160
117 106 85 9
BspEI T'CCGG_A
Cuts at: 5157 5157
Size: 5494
BspHI T'CATG_A
Cuts at: 3383 5113 3383
Size: 1730 3764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2227 2849 844
Size: 1383 622 3489
Fragments arranged by size:
3489 1383 622
BsrBI CCG'CTC
Cuts at: 1394 1823 3381 1394
Size: 429 1558 3507
Fragments arranged by size:
3507 1558 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3989 4163 65
Size: 1183 2741 174 1396
Fragments arranged by size:
2741 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2722 4138 4325 1700
Size: 110 912 1416 187 2869
Fragments arranged by size:
2869 1416 912 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5494
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5494
BssSI C'ACGA_G
Cuts at: 3554 4566 3554
Size: 1012 4482
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5494
BstBI TT'CG_AA
Cuts at: 4298 4298
Size: 5494
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5494
BstYI r'GATC_y
Cuts at: 1972 2824 3191 3576 3593 5045 5131 5143 5489
Size: 852 367 385 17 1452 86 12 346
Cuts at: 5489 1972
Size: 1977
Fragments arranged by size:
1977 1452 852 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2319 2565 513
Size: 552 1254 246 3442
Fragments arranged by size:
3442 1254 552 246
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4755
Fragments arranged by size:
4755 683 56
BtsI GCAGTG_nn'
Cuts at: 2017 2040 3010 3804 3824 2017
Size: 23 970 794 20 3687
Fragments arranged by size:
3687 970 794 23 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5494
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5459
DraI TTT'AAA
Cuts at: 2798 2798
Size: 5494
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4501 809
Size: 507 3185 1802
Fragments arranged by size:
3185 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2487 2613 2622
Size: 54 1425 50 275 18 657 126 9
Cuts at: 2622 2941 3830 5459 8
Size: 319 889 1629 43
Fragments arranged by size:
1629 1425 889 657 319 275 126 54
50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2941 1537
Size: 275 1129 4090
Fragments arranged by size:
4090 1129 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2068 2133 2190 2780 3424 1353
Size: 715 65 57 590 644 3423
Fragments arranged by size:
3423 715 644 590 65 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4081 4455 4601 387
Size: 1064 138 2492 374 146 1280
Fragments arranged by size:
2492 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2200 2200
Size: 5494
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2325 2423 3557 4154 4941 5209
Size: 393 482 211 98 1134 597 787 268
Cuts at: 5209 1239
Size: 1524
Fragments arranged by size:
1524 1134 787 597 482 393 268 211
98
EcoO109I rG'GnC_Cy
Cuts at: 2286 2435 2444 2533 2286
Size: 149 9 89 5247
Fragments arranged by size:
5247 149 89 9
EcoRI G'AATT_C
Cuts at: 2803 3181 2803
Size: 378 5116
FspI TGC'GCA
Cuts at: 2633 4000 2633
Size: 1367 4127
HaeII r_GCGC'y
Cuts at: 1678 2107 2402 2729 4641 1678
Size: 429 295 327 1912 2531
Fragments arranged by size:
2531 1912 429 327 295
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2110 2142 2323 2355 4135
Size: 32 135 32 538 32 181 32 1780
Cuts at: 4135 4167 4209 4241 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1780 538 181 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2838 3084 669
Size: 988 281 900 246 3079
Fragments arranged by size:
3079 988 900 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5494
HpaI GTT'AAC
Cuts at: 3084 3084
Size: 5494
KasI G'GCGC_C
Cuts at: 2725 2725
Size: 5494
KpnI G_GTAC'C
Cuts at: 2819 2819
Size: 5494
MfeI C'AATT_G
Cuts at: 3093 3093
Size: 5494
MluI A'CGCG_T
Cuts at: 5370 5370
Size: 5494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4608 4792 1305
Size: 3303 184 2007
Fragments arranged by size:
3303 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2624 10
Size: 54 1425 343 792 2880
Fragments arranged by size:
2880 1425 792 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3452 3811 3970 518
Size: 648 2286 359 159 2042
Fragments arranged by size:
2286 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2137 2910 3587 4735 4980 1606
Size: 531 773 677 1148 245 2120
Fragments arranged by size:
2120 1148 773 677 531 245
NaeI GCC'GGC
Cuts at: 2724 2724
Size: 5494
NarI GG'CG_CC
Cuts at: 2726 2726
Size: 5494
NcoI C'CATG_G
Cuts at: 513 1065 2319 2565 513
Size: 552 1254 246 3442
Fragments arranged by size:
3442 1254 552 246
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5494
NgoMIV G'CCGG_C
Cuts at: 2722 2722
Size: 5494
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5494
NotI GC'GGCC_GC
Cuts at: 2941 2941
Size: 5494
NspI r_CATG'y
Cuts at: 1132 1229 2852 4397 1132
Size: 97 1623 1545 2229
Fragments arranged by size:
2229 1623 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2369 1348
Size: 1021 4473
PciI A'CATG_T
Cuts at: 4393 4393
Size: 5494
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5494
PmeI GTTT'AAAC
Cuts at: 2798 2798
Size: 5494
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3563 3595 5076 5108 3563
Size: 32 1481 32 3949
Fragments arranged by size:
3949 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2435 2435
Size: 5494
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5494
PsiI TTA'TAA
Cuts at: 3064 3064
Size: 5494
PspOMI G'GGCC_C
Cuts at: 2533 2533
Size: 5494
PstI C_TGCA'G
Cuts at: 830 2443 2670 2846 830
Size: 1613 227 176 3478
Fragments arranged by size:
3478 1613 227 176
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5494
PvuII CAG'CTG
Cuts at: 2137 2137
Size: 5494
SacI G_AGCT'C
Cuts at: 2202 2202
Size: 5494
SalI G'TCGA_C
Cuts at: 1936 2836 1936
Size: 900 4594
SapI GCTCTTCn'nnn_
Cuts at: 2190 2190
Size: 5494
SbfI CC_TGCA'GG
Cuts at: 2443 2846 2443
Size: 403 5091
ScaI AGT'ACT
Cuts at: 3742 5454 3742
Size: 1712 3782
SfcI C'TryA_G
Cuts at: 826 1045 2439 2666 2842 3977 4658 4849 826
Size: 219 1394 227 176 1135 681 191 1471
Fragments arranged by size:
1471 1394 1135 681 227 219 191 176
SfiI GGCCn_nnn'nGGCC
Cuts at: 2621 2621
Size: 5494
SfoI GGC'GCC
Cuts at: 2727 2727
Size: 5494
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5494
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5494
SmaI CCC'GGG
Cuts at: 1872 2821 1872
Size: 949 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2147 3598 4499 4761 820
Size: 197 926 204 1451 901 262 1553
Fragments arranged by size:
1553 1451 926 901 262 204 197
SnaBI TAC'GTA
Cuts at: 493 3285 493
Size: 2792 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5494
SphI G_CATG'C
Cuts at: 1132 2852 1132
Size: 1720 3774
SspI AAT'ATT
Cuts at: 5 52 3418 5
Size: 47 3366 2081
Fragments arranged by size:
3366 2081 47
StuI AGG'CCT
Cuts at: 1517 2504 1517
Size: 987 4507
StyI C'CwwG_G
Cuts at: 513 1065 2310 2319 2565 2763 2933 513
Size: 552 1245 9 246 198 170 3074
Fragments arranged by size:
3074 1245 552 246 198 170 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3521 3538 3691 3876 4321 325
Size: 1255 1941 17 153 185 445 1498
Fragments arranged by size:
1941 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3740 5452 96
Size: 274 80 33 51 1443 1763 1712 138
Fragments arranged by size:
1763 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5454 5486 5454
Size: 32 5462
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5494
XbaI T'CTAG_A
Cuts at: 2830 2830
Size: 5494
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2317 2317
Size: 5494
XhoI C'TCGA_G
Cuts at: 1943 2147 1943
Size: 204 5290
XmaI C'CCGG_G
Cuts at: 1870 2819 1870
Size: 949 4545
XmnI GAAnn'nnTTC
Cuts at: 3623 3623
Size: 5494
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3302 276
Size: 53 83 186 936 1768 2468
Fragments arranged by size:
2468 1768 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacII SanDI SexAI SrfI SwaI