Restriction map for
pFN21AE0669
(Circular) (Six-base) MAPSORT of: pFN21AE0669.seq Check: 6554 from: 1 to: 5452
pFN21AE0669 5452 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3262 278
Size: 53 83 186 936 1726 2468
Fragments arranged by size:
2468 1726 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2795 1937
Size: 858 4594
Acc65I G'GTAC_C
Cuts at: 2773 2773
Size: 5452
AclI AA'CG_TT
Cuts at: 1563 3579 3952 1563
Size: 2016 373 3063
Fragments arranged by size:
3063 2016 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2578 3515 4899 5167 1632
Size: 946 937 1384 268 1917
Fragments arranged by size:
1917 1384 946 937 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5255
AflIII A'CryG_T
Cuts at: 1203 4351 5328 1203
Size: 3148 977 1327
Fragments arranged by size:
3148 1327 977
AgeI A'CCGG_T
Cuts at: 4283 4283
Size: 5452
AhdI GACnn_n'nnGTC
Cuts at: 4181 4181
Size: 5452
AleI CACnn'nnGTG
Cuts at: 1166 2276 1166
Size: 1110 4342
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2294 2432 1184
Size: 1110 138 4204
Fragments arranged by size:
4204 1110 138
AlwNI CAG_nnn'CTG
Cuts at: 2383 2440 3195 4767 2383
Size: 57 755 1572 3068
Fragments arranged by size:
3068 1572 755 57
ApaI G_GGCC'C
Cuts at: 2658 2716 2658
Size: 58 5394
ApaLI G'TGCA_C
Cuts at: 3509 4665 3509
Size: 1156 4296
ApoI r'AATT_y
Cuts at: 1493 2761 2987 3139 5248 1493
Size: 1268 226 152 2109 1697
Fragments arranged by size:
2109 1697 1268 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4006 160
Size: 872 2974 1606
Fragments arranged by size:
2974 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5452
AvaI C'yCGr_G
Cuts at: 1870 1943 2262 2606 2777 1870
Size: 73 319 344 171 4545
Fragments arranged by size:
4545 344 319 171 73
BamHI G'GATC_C
Cuts at: 2782 3149 2782
Size: 367 5085
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2644 2773 4228 618
Size: 325 217 627 857 129 1455 1842
Fragments arranged by size:
1842 1455 857 627 325 217 129
BanII G_rGCy'C
Cuts at: 1391 2175 2613 2658 2716 2771 1391
Size: 784 438 45 58 55 4072
Fragments arranged by size:
4072 784 438 58 55 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4481
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2141 2175 3643 3677 1903
Size: 34 204 34 1468 34 3678
Fragments arranged by size:
3678 1468 204 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3344 4554 3344
Size: 1210 4242
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5452
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2533 4063 136
Size: 107 122 71 631 605 861 1530 1525
Fragments arranged by size:
1530 1525 861 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5447 5447
Size: 5452
BlpI GC'TnA_GC
Cuts at: 2732 2869 2732
Size: 137 5315
Bme1580I G_kGCm'C
Cuts at: 1790 2545 2658 2716 3513 4669 1790
Size: 755 113 58 797 1156 2573
Fragments arranged by size:
2573 1156 797 755 113 58
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5452
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2428 2471 2532
Size: 416 53 83 399 24 1011 43 61
Cuts at: 2532 3183 4141 442
Size: 651 958 1753
Fragments arranged by size:
1753 1011 958 651 416 399 83 61
53 43 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5452
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 2393 2596 4112 1239
Size: 974 180 203 1516 2579
Fragments arranged by size:
2579 1516 974 203 180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3577 4442 4740 3577
Size: 865 298 4289
Fragments arranged by size:
4289 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4115 882
Size: 617 2616 2219
Fragments arranged by size:
2616 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2278 2396 3243 493
Size: 711 1074 118 847 2702
Fragments arranged by size:
2702 1074 847 711 118
BsaBI GATnn'nnATC
Cuts at: 5446 5446
Size: 5452
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2049 2181 3259 3641
Size: 53 83 186 936 516 132 1078 382
Cuts at: 3641 4276 275
Size: 635 1451
Fragments arranged by size:
1451 1078 936 635 516 382 186 132
83 53
BsaWI w'CCGG_w
Cuts at: 3885 4283 4557 4704 5115 3885
Size: 398 274 147 411 4222
Fragments arranged by size:
4222 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5227
Fragments arranged by size:
5227 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2243 1186
Size: 1057 4395
BseYI C'CCAG_C
Cuts at: 2313 2421 2427 2500 2512 4655 5151 2313
Size: 108 6 73 12 2143 496 2614
Fragments arranged by size:
2614 2143 496 108 73 12 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2185 2669 1163
Size: 573 449 484 3946
Fragments arranged by size:
3946 573 484 449
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2902 3663 4691 1540
Size: 275 186 901 761 1028 2301
Fragments arranged by size:
2301 1028 901 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2582 3513 3598 4669 2582
Size: 931 85 1071 3365
Fragments arranged by size:
3365 1071 931 85
BsmI GAATG_Cn'
Cuts at: 2963 3056 2963
Size: 93 5359
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2175 2545 2582 2613 2658 2716 2771
Size: 399 385 370 37 31 45 58 55
Cuts at: 2771 3513 3598 4669 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 385 370 85 58
55 45 37 31
BspEI T'CCGG_A
Cuts at: 5115 5115
Size: 5452
BspHI T'CATG_A
Cuts at: 3341 5071 3341
Size: 1730 3722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2358 2807 844
Size: 1514 449 3489
Fragments arranged by size:
3489 1514 449
BsrBI CCG'CTC
Cuts at: 1394 1823 3339 1394
Size: 429 1516 3507
Fragments arranged by size:
3507 1516 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3947 4121 65
Size: 1183 2699 174 1396
Fragments arranged by size:
2699 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2109 2532 4096 4283 1700
Size: 110 299 423 1564 187 2869
Fragments arranged by size:
2869 1564 423 299 187 110
BsrGI T'GTAC_A
Cuts at: 96 2076 96
Size: 1980 3472
BssHII G'CGCG_C
Cuts at: 1925 2267 1925
Size: 342 5110
BssSI C'ACGA_G
Cuts at: 2687 3512 4524 2687
Size: 825 1012 3615
Fragments arranged by size:
3615 1012 825
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5452
BstBI TT'CG_AA
Cuts at: 4256 4256
Size: 5452
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5452
BstYI r'GATC_y
Cuts at: 1972 2706 2782 3149 3534 3551 5003 5089 5101
Size: 734 76 367 385 17 1452 86 12
Cuts at: 5101 5447 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 734 385 367 346 86 76
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2175 2304 2650 513
Size: 552 1110 129 346 3315
Fragments arranged by size:
3315 1110 552 346 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4713
Fragments arranged by size:
4713 683 56
BtsI GCAGTG_nn'
Cuts at: 2968 3762 3782 2968
Size: 794 20 4638
Fragments arranged by size:
4638 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5452
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2068 2103 529
Size: 35 1504 35 3878
Fragments arranged by size:
3878 1504 35 35
DraI TTT'AAA
Cuts at: 2756 2756
Size: 5452
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4459 809
Size: 507 3143 1802
Fragments arranged by size:
3143 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2283 2698 2722
Size: 54 1425 50 275 18 453 415 24
Cuts at: 2722 2899 3788 5417 8
Size: 177 889 1629 43
Fragments arranged by size:
1629 1425 889 453 415 275 177 54
50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2899 1537
Size: 275 1087 4090
Fragments arranged by size:
4090 1087 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2147 3382 1353
Size: 794 1235 3423
Fragments arranged by size:
3423 1235 794
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4039 4413 4559 387
Size: 1064 138 2450 374 146 1280
Fragments arranged by size:
2450 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2393 2578 2596 3515 4112 4899
Size: 393 581 180 185 18 919 597 787
Cuts at: 4899 5167 1239
Size: 268 1524
Fragments arranged by size:
1524 919 787 597 581 393 268 185
180 18
EcoO109I rG'GnC_Cy
Cuts at: 2259 2323 2655 2713 2259
Size: 64 332 58 4998
Fragments arranged by size:
4998 332 64 58
EcoRI G'AATT_C
Cuts at: 2761 3139 2761
Size: 378 5074
FspI TGC'GCA
Cuts at: 3958 3958
Size: 5452
HaeII r_GCGC'y
Cuts at: 1678 2116 4599 1678
Size: 438 2483 2531
Fragments arranged by size:
2531 2483 438
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4093 4125 4167 4199 1373
Size: 32 135 32 2521 32 42 32 2626
Fragments arranged by size:
2626 2521 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2796 3042 669
Size: 988 281 858 246 3079
Fragments arranged by size:
3079 988 858 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5452
HpaI GTT'AAC
Cuts at: 3042 3042
Size: 5452
KpnI G_GTAC'C
Cuts at: 2777 2777
Size: 5452
MfeI C'AATT_G
Cuts at: 3051 3051
Size: 5452
MluI A'CGCG_T
Cuts at: 5328 5328
Size: 5452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4566 4750 1305
Size: 3261 184 2007
Fragments arranged by size:
3261 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2700 2724 10
Size: 54 1425 343 868 24 2738
Fragments arranged by size:
2738 1425 868 343 54 24
MslI CAynn'nnrTG
Cuts at: 518 1166 2276 3410 3769 3928 518
Size: 648 1110 1134 359 159 2042
Fragments arranged by size:
2042 1134 1110 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2288 2383 2868 3545 4693 4938 1606
Size: 682 95 485 677 1148 245 2120
Fragments arranged by size:
2120 1148 682 677 485 245 95
NaeI GCC'GGC
Cuts at: 2534 2534
Size: 5452
NcoI C'CATG_G
Cuts at: 513 1065 2304 513
Size: 552 1239 3661
Fragments arranged by size:
3661 1239 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5452
NgoMIV G'CCGG_C
Cuts at: 2532 2532
Size: 5452
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5452
NotI GC'GGCC_GC
Cuts at: 2899 2899
Size: 5452
NspI r_CATG'y
Cuts at: 1132 1229 2140 2810 4355 1132
Size: 97 911 670 1545 2229
Fragments arranged by size:
2229 1545 911 670 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5452
PciI A'CATG_T
Cuts at: 4351 4351
Size: 5452
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5452
PfoI T'CCnGG_A
Cuts at: 2190 2190
Size: 5452
PmeI GTTT'AAAC
Cuts at: 2756 2756
Size: 5452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3521 3553 5034 5066 3521
Size: 32 1481 32 3907
Fragments arranged by size:
3907 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2259 2259
Size: 5452
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5452
PsiI TTA'TAA
Cuts at: 3022 3022
Size: 5452
PspOMI G'GGCC_C
Cuts at: 2654 2712 2654
Size: 58 5394
PstI C_TGCA'G
Cuts at: 830 2804 830
Size: 1974 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5452
PvuII CAG'CTG
Cuts at: 2383 2383
Size: 5452
SalI G'TCGA_C
Cuts at: 1936 2794 1936
Size: 858 4594
SanDI GG'GwC_CC
Cuts at: 2259 2259
Size: 5452
SapI GCTCTTCn'nnn_
Cuts at: 2147 2147
Size: 5452
SbfI CC_TGCA'GG
Cuts at: 2804 2804
Size: 5452
ScaI AGT'ACT
Cuts at: 3700 5412 3700
Size: 1712 3740
SfcI C'TryA_G
Cuts at: 826 1045 2800 3935 4616 4807 826
Size: 219 1755 1135 681 191 1471
Fragments arranged by size:
1755 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5452
SgrAI Cr'CCGG_yG
Cuts at: 1700 2109 1700
Size: 409 5043
SmaI CCC'GGG
Cuts at: 1872 2264 2779 1872
Size: 392 515 4545
Fragments arranged by size:
4545 515 392
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3556 4457 4719 820
Size: 197 926 1613 901 262 1553
Fragments arranged by size:
1613 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3243 493
Size: 2750 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5452
SphI G_CATG'C
Cuts at: 1132 2810 1132
Size: 1678 3774
SspI AAT'ATT
Cuts at: 5 52 3376 5
Size: 47 3324 2081
Fragments arranged by size:
3324 2081 47
StuI AGG'CCT
Cuts at: 1517 2599 1517
Size: 1082 4370
StyI C'CwwG_G
Cuts at: 513 1065 2304 2553 2891 513
Size: 552 1239 249 338 3074
Fragments arranged by size:
3074 1239 552 338 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3479 3496 3649 3834 4279 325
Size: 1255 1899 17 153 185 445 1498
Fragments arranged by size:
1899 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2076 3698 5410
Size: 274 80 33 51 1443 99 1622 1712
Cuts at: 5410 96
Size: 138
Fragments arranged by size:
1712 1622 1443 274 138 99 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5412 5444 5412
Size: 32 5420
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5452
XbaI T'CTAG_A
Cuts at: 2788 2788
Size: 5452
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5452
XmaI C'CCGG_G
Cuts at: 1870 2262 2777 1870
Size: 392 515 4545
Fragments arranged by size:
4545 515 392
XmnI GAAnn'nnTTC
Cuts at: 3581 3581
Size: 5452
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3260 276
Size: 53 83 186 936 1726 2468
Fragments arranged by size:
2468 1726 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI