Restriction map for
pFN21AE0665
(Circular) (Six-base) MAPSORT of: pFN21AE0665.seq Check: 1542 from: 1 to: 5425
pFN21AE0665 5425 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3235 278
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2768 1937
Size: 831 4594
Acc65I G'GTAC_C
Cuts at: 2746 2746
Size: 5425
AclI AA'CG_TT
Cuts at: 1563 3552 3925 1563
Size: 1989 373 3063
Fragments arranged by size:
3063 1989 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2437 3488 4872 5140 1632
Size: 805 1051 1384 268 1917
Fragments arranged by size:
1917 1384 1051 805 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5228
AflIII A'CryG_T
Cuts at: 1203 2340 4324 5301 1203
Size: 1137 1984 977 1327
Fragments arranged by size:
1984 1327 1137 977
AgeI A'CCGG_T
Cuts at: 4256 4256
Size: 5425
AhdI GACnn_n'nnGTC
Cuts at: 4154 4154
Size: 5425
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5425
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2534 1184
Size: 1350 4075
AlwNI CAG_nnn'CTG
Cuts at: 3168 4740 3168
Size: 1572 3853
ApaLI G'TGCA_C
Cuts at: 3482 4638 3482
Size: 1156 4269
ApoI r'AATT_y
Cuts at: 1493 2734 2960 3112 5221 1493
Size: 1241 226 152 2109 1697
Fragments arranged by size:
2109 1697 1241 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3979 160
Size: 872 2947 1606
Fragments arranged by size:
2947 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
AvaI C'yCGr_G
Cuts at: 1870 1943 2601 2750 1870
Size: 73 658 149 4545
Fragments arranged by size:
4545 658 149 73
BamHI G'GATC_C
Cuts at: 2755 3122 2755
Size: 367 5058
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 2746 4201 618
Size: 325 217 627 246 713 1455 1842
Fragments arranged by size:
1842 1455 713 627 325 246 217
BanII G_rGCy'C
Cuts at: 1391 2744 1391
Size: 1353 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4454
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3616 3650 1903
Size: 34 1679 34 3678
Fragments arranged by size:
3678 1679 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3317 4527 3317
Size: 1210 4215
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5425
BfrBI ATG'CAT
Cuts at: 2521 2521
Size: 5425
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4036 136
Size: 107 122 71 631 605 2364 1525
Fragments arranged by size:
2364 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2711 5420 2711
Size: 2709 2716
BlpI GC'TnA_GC
Cuts at: 2842 2842
Size: 5425
Bme1580I G_kGCm'C
Cuts at: 1790 3486 4642 1790
Size: 1696 1156 2573
Fragments arranged by size:
2573 1696 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5425
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2176 3156 4114
Size: 416 53 83 399 24 759 980 958
Cuts at: 4114 442
Size: 1753
Fragments arranged by size:
1753 980 958 759 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4085 1239
Size: 2846 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3550 4415 4713 3550
Size: 865 298 4262
Fragments arranged by size:
4262 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2316 2619 4088 882
Size: 617 817 303 1469 2219
Fragments arranged by size:
2219 1469 817 617 303
BsaAI yAC'GTr
Cuts at: 493 1204 3216 493
Size: 711 2012 2702
Fragments arranged by size:
2702 2012 711
BsaBI GATnn'nnATC
Cuts at: 2590 2596 5419 2590
Size: 6 2823 2596
Fragments arranged by size:
2823 2596 6
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3232 3614 4249 275
Size: 53 83 186 936 1699 382 635 1451
Fragments arranged by size:
1699 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3858 4256 4530 4677 5088 3858
Size: 398 274 147 411 4195
Fragments arranged by size:
4195 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2264 2294 1178
Size: 30 165 30 861 30 4309
Fragments arranged by size:
4309 861 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2267 2288 2309 1186
Size: 1081 21 21 4302
Fragments arranged by size:
4302 1081 21 21
BseYI C'CCAG_C
Cuts at: 4628 5124 4628
Size: 496 4929
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4852
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2696 2875 3636 4664 1540
Size: 275 186 695 179 761 1028 2301
Fragments arranged by size:
2301 1028 761 695 275 186 179
BsiHKAI G_wGCw'C
Cuts at: 2105 3486 3571 4642 2105
Size: 1381 85 1071 2888
Fragments arranged by size:
2888 1381 1071 85
BsmI GAATG_Cn'
Cuts at: 2495 2936 3029 2495
Size: 441 93 4891
Fragments arranged by size:
4891 441 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2105 2744 3486 3571 4642 1391
Size: 399 315 639 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 639 399 315 85
BspEI T'CCGG_A
Cuts at: 5088 5088
Size: 5425
BspHI T'CATG_A
Cuts at: 3314 5044 3314
Size: 1730 3695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2780 844
Size: 1936 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3312 1394
Size: 429 1489 3507
Fragments arranged by size:
3507 1489 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2139 3920 4094 65
Size: 1183 891 1781 174 1396
Fragments arranged by size:
1781 1396 1183 891 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4069 4256 1700
Size: 110 2259 187 2869
Fragments arranged by size:
2869 2259 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5425
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5425
BssSI C'ACGA_G
Cuts at: 2496 3485 4497 2496
Size: 989 1012 3424
Fragments arranged by size:
3424 1012 989
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5425
BstBI TT'CG_AA
Cuts at: 4229 4229
Size: 5425
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5425
BstYI r'GATC_y
Cuts at: 1972 2591 2711 2755 3122 3507 3524 4976 5062
Size: 619 120 44 367 385 17 1452 86
Cuts at: 5062 5074 5420 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 619 385 367 346 120 86
44 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2629 513
Size: 552 939 625 3309
Fragments arranged by size:
3309 939 625 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2229 487
Size: 683 56 1003 3683
Fragments arranged by size:
3683 1003 683 56
BtsI GCAGTG_nn'
Cuts at: 2941 3735 3755 2941
Size: 794 20 4611
Fragments arranged by size:
4611 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5425
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5390
DraI TTT'AAA
Cuts at: 2729 2729
Size: 5425
DraIII CAC_nnn'GTG
Cuts at: 2332 2332
Size: 5425
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4432 809
Size: 507 3116 1802
Fragments arranged by size:
3116 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2563 2872 3761
Size: 54 1425 50 275 18 733 309 889
Cuts at: 3761 5390 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 733 309 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2872 1537
Size: 275 1060 4090
Fragments arranged by size:
4090 1060 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2182 3355 1353
Size: 829 1173 3423
Fragments arranged by size:
3423 1173 829
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2685 4012 4386 4532 387
Size: 1064 138 435 661 1327 374 146 1280
Fragments arranged by size:
1327 1280 1064 661 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2437 3488 4085 4872 5140 1239
Size: 393 805 1051 597 787 268 1524
Fragments arranged by size:
1524 1051 805 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2734 3112 2734
Size: 378 5047
FspI TGC'GCA
Cuts at: 3931 3931
Size: 5425
HaeII r_GCGC'y
Cuts at: 1678 4572 1678
Size: 2894 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2087 2119 2406 2438 4066
Size: 32 135 32 515 32 287 32 1628
Cuts at: 4066 4098 4140 4172 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1628 515 287 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2053 2769 3015 669
Size: 988 281 115 716 246 3079
Fragments arranged by size:
3079 988 716 281 246 115
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5425
HpaI GTT'AAC
Cuts at: 3015 3015
Size: 5425
KpnI G_GTAC'C
Cuts at: 2750 2750
Size: 5425
MfeI C'AATT_G
Cuts at: 3024 3024
Size: 5425
MluI A'CGCG_T
Cuts at: 5301 5301
Size: 5425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4539 4723 1305
Size: 3234 184 2007
Fragments arranged by size:
3234 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3603
Fragments arranged by size:
3603 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2238 3383 3742 3901 518
Size: 648 1072 1145 359 159 2042
Fragments arranged by size:
2042 1145 1072 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2841 3518 4666 4911 1606
Size: 1235 677 1148 245 2120
Fragments arranged by size:
2120 1235 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3934
Fragments arranged by size:
3934 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5425
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5425
NotI GC'GGCC_GC
Cuts at: 2872 2872
Size: 5425
NsiI A_TGCA'T
Cuts at: 2523 2523
Size: 5425
NspI r_CATG'y
Cuts at: 1132 1229 2085 2783 4328 1132
Size: 97 856 698 1545 2229
Fragments arranged by size:
2229 1545 856 698 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5425
PciI A'CATG_T
Cuts at: 4324 4324
Size: 5425
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2332 1108
Size: 1224 4201
PmeI GTTT'AAAC
Cuts at: 2729 2729
Size: 5425
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3494 3526 5007 5039 3494
Size: 32 1481 32 3880
Fragments arranged by size:
3880 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2631 1613
Size: 1018 4407
PsiI TTA'TAA
Cuts at: 2995 2995
Size: 5425
PstI C_TGCA'G
Cuts at: 830 2777 830
Size: 1947 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5425
SalI G'TCGA_C
Cuts at: 1936 2767 1936
Size: 831 4594
SbfI CC_TGCA'GG
Cuts at: 2777 2777
Size: 5425
ScaI AGT'ACT
Cuts at: 3673 5385 3673
Size: 1712 3713
SexAI A'CCwGG_T
Cuts at: 2328 2328
Size: 5425
SfcI C'TryA_G
Cuts at: 826 1045 2773 3908 4589 4780 826
Size: 219 1728 1135 681 191 1471
Fragments arranged by size:
1728 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5425
SmaI CCC'GGG
Cuts at: 1872 2603 2752 1872
Size: 731 149 4545
Fragments arranged by size:
4545 731 149
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3529 4430 4692 820
Size: 197 926 1586 901 262 1553
Fragments arranged by size:
1586 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3216 493
Size: 2723 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5425
SphI G_CATG'C
Cuts at: 1132 2783 1132
Size: 1651 3774
SspI AAT'ATT
Cuts at: 5 52 3349 5
Size: 47 3297 2081
Fragments arranged by size:
3297 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5425
StyI C'CwwG_G
Cuts at: 513 1065 2004 2864 513
Size: 552 939 860 3074
Fragments arranged by size:
3074 939 860 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2607 3452 3469 3622 3807 4252 325
Size: 1255 1027 845 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1027 845 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3671 5383 96
Size: 274 80 33 51 1443 1694 1712 138
Fragments arranged by size:
1712 1694 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5385 5417 5385
Size: 32 5393
Tth111I GACn'n_nGTC
Cuts at: 1316 2048 1316
Size: 732 4693
XbaI T'CTAG_A
Cuts at: 2621 2761 2621
Size: 140 5285
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5425
XmaI C'CCGG_G
Cuts at: 1870 2601 2750 1870
Size: 731 149 4545
Fragments arranged by size:
4545 731 149
XmnI GAAnn'nnTTC
Cuts at: 3554 3554
Size: 5425
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3233 276
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI XcmI