Restriction map for
pFN21AE0664
(Circular) (Six-base) MAPSORT of: pFN21AE0664.seq Check: 2121 from: 1 to: 5881
pFN21AE0664 5881 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3691 278
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3224 1937
Size: 1287 4594
Acc65I G'GTAC_C
Cuts at: 3202 3202
Size: 5881
AclI AA'CG_TT
Cuts at: 1563 4008 4381 1563
Size: 2445 373 3063
Fragments arranged by size:
3063 2445 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3944 5328 5596 1632
Size: 2312 1384 268 1917
Fragments arranged by size:
2312 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5684
AflIII A'CryG_T
Cuts at: 1203 4780 5757 1203
Size: 3577 977 1327
Fragments arranged by size:
3577 1327 977
AgeI A'CCGG_T
Cuts at: 4712 4712
Size: 5881
AhdI GACnn_n'nnGTC
Cuts at: 4610 4610
Size: 5881
AleI CACnn'nnGTG
Cuts at: 1166 2742 1166
Size: 1576 4305
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5881
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2204 2236 2204
Size: 32 5849
AlwNI CAG_nnn'CTG
Cuts at: 3624 5196 3624
Size: 1572 4309
ApaI G_GGCC'C
Cuts at: 2291 2385 2437 2510 2548 2630 2291
Size: 94 52 73 38 82 5542
Fragments arranged by size:
5542 94 82 73 52 38
ApaLI G'TGCA_C
Cuts at: 3938 5094 3938
Size: 1156 4725
ApoI r'AATT_y
Cuts at: 1493 3190 3416 3568 5677 1493
Size: 1697 226 152 2109 1697
Fragments arranged by size:
2109 1697 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4435 160
Size: 872 3403 1606
Fragments arranged by size:
3403 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
AvaI C'yCGr_G
Cuts at: 1870 1943 2023 2317 2373 2665 2688 3013 3206
Size: 73 80 294 56 292 23 325 193
Cuts at: 3206 1870
Size: 4545
Fragments arranged by size:
4545 325 294 292 193 80 73 56
23
BamHI G'GATC_C
Cuts at: 3211 3578 3211
Size: 367 5514
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2876 3118 3202 4657 618
Size: 325 217 627 1089 242 84 1455 1842
Fragments arranged by size:
1842 1455 1089 627 325 242 217 84
BanII G_rGCy'C
Cuts at: 1391 2291 2385 2437 2510 2548 2630 2850 3054
Size: 900 94 52 73 38 82 220 204
Cuts at: 3054 3200 1391
Size: 146 4072
Fragments arranged by size:
4072 900 220 204 146 94 82 73
52 38
BbeI G_GCGC'C
Cuts at: 2880 2880
Size: 5881
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4910
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2308 2342 4072 4106 1903
Size: 34 371 34 1730 34 3678
Fragments arranged by size:
3678 1730 371 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3773 4983 3773
Size: 1210 4671
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5881
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2286 3108 4492
Size: 107 122 71 631 605 614 822 1384
Cuts at: 4492 136
Size: 1525
Fragments arranged by size:
1525 1384 822 631 614 605 122 107
71
BglII A'GATC_T
Cuts at: 5876 5876
Size: 5881
BlpI GC'TnA_GC
Cuts at: 3298 3298
Size: 5881
Bme1580I G_kGCm'C
Cuts at: 1790 2291 2385 2437 2510 2548 2630 3123 3942
Size: 501 94 52 73 38 82 493 819
Cuts at: 3942 5098 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 819 501 493 94 82 73
52 38
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5881
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3612 4570 442
Size: 416 53 83 399 24 2195 958 1753
Fragments arranged by size:
2195 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5881
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2662 2662
Size: 5881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2413 2725 4541 1239
Size: 1174 312 1816 2579
Fragments arranged by size:
2579 1816 1174 312
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2785 4006 4871 5169 2785
Size: 1221 865 298 3497
Fragments arranged by size:
3497 1221 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4544 882
Size: 617 3045 2219
Fragments arranged by size:
3045 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3672 493
Size: 711 2468 2702
Fragments arranged by size:
2702 2468 711
BsaBI GATnn'nnATC
Cuts at: 5875 5875
Size: 5881
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2187 2877 3688 4070
Size: 53 83 186 936 654 690 811 382
Cuts at: 4070 4705 275
Size: 635 1451
Fragments arranged by size:
1451 936 811 690 654 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4314 4712 4986 5133 5544 4314
Size: 398 274 147 411 4651
Fragments arranged by size:
4651 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2723 2753 1178
Size: 30 165 30 1320 30 4306
Fragments arranged by size:
4306 1320 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2928 2931 2945 3174 3177 1186
Size: 1742 3 14 229 3 3890
Fragments arranged by size:
3890 1742 229 14 3 3
BseYI C'CCAG_C
Cuts at: 3084 5084 5580 3084
Size: 2000 496 3385
Fragments arranged by size:
3385 2000 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5308
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2314 2782 2806 2971 3007 3331
Size: 275 186 313 468 24 165 36 324
Cuts at: 3331 4092 5120 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 468 324 313 275 186
165 36 24
BsiHKAI G_wGCw'C
Cuts at: 2118 3942 4027 5098 2118
Size: 1824 85 1071 2901
Fragments arranged by size:
2901 1824 1071 85
BsmI GAATG_Cn'
Cuts at: 2538 3392 3485 2538
Size: 854 93 4934
Fragments arranged by size:
4934 854 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2559 2649 2778 2559
Size: 90 129 5662
Fragments arranged by size:
5662 129 90
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2291 2385 2437 2510 2548 2630
Size: 399 328 173 94 52 73 38 82
Cuts at: 2630 2850 3054 3123 3200 3942 4027 5098 1391
Size: 220 204 69 77 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 328 220 204 173
94 85 82 77 73 69 52 38
BspEI T'CCGG_A
Cuts at: 5544 5544
Size: 5881
BspHI T'CATG_A
Cuts at: 3770 5500 3770
Size: 1730 4151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2427 3236 844
Size: 1583 809 3489
Fragments arranged by size:
3489 1583 809
BsrBI CCG'CTC
Cuts at: 1394 1823 3768 1394
Size: 429 1945 3507
Fragments arranged by size:
3507 1945 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4376 4550 65
Size: 1183 3128 174 1396
Fragments arranged by size:
3128 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2263 2280 3102 4525 4712 1700
Size: 110 453 17 822 1423 187 2869
Fragments arranged by size:
2869 1423 822 453 187 110 17
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5881
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5881
BssSI C'ACGA_G
Cuts at: 3941 4953 3941
Size: 1012 4869
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5881
BstBI TT'CG_AA
Cuts at: 4685 4685
Size: 5881
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5881
BstXI CCAn_nnnn'nTGG
Cuts at: 2245 2245
Size: 5881
BstYI r'GATC_y
Cuts at: 1972 3211 3578 3963 3980 5432 5518 5530 5876
Size: 1239 367 385 17 1452 86 12 346
Cuts at: 5876 1972
Size: 1977
Fragments arranged by size:
1977 1452 1239 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2308 2338 2357 2630 3063 513
Size: 552 939 304 30 19 273 433 3331
Fragments arranged by size:
3331 939 552 433 304 273 30 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5142
Fragments arranged by size:
5142 683 56
BtsI GCAGTG_nn'
Cuts at: 3397 4191 4211 3397
Size: 794 20 5067
Fragments arranged by size:
5067 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5881
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5846
DraI TTT'AAA
Cuts at: 3185 3185
Size: 5881
DraIII CAC_nnn'GTG
Cuts at: 3031 3031
Size: 5881
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4888 809
Size: 507 3572 1802
Fragments arranged by size:
3572 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 2550 2734
Size: 54 1425 50 275 18 268 452 184
Cuts at: 2734 2968 2995 3328 4217 5846 8
Size: 234 27 333 889 1629 43
Fragments arranged by size:
1629 1425 889 452 333 275 268 234
184 54 50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2968 3328 1537
Size: 275 1156 360 4090
Fragments arranged by size:
4090 1156 360 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3811 1353
Size: 2458 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2423 3144 4468 4842 4988 387
Size: 1064 138 834 721 1324 374 146 1280
Fragments arranged by size:
1324 1280 1064 834 721 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2413 2725 3944 4541 5328 5596 1239
Size: 393 781 312 1219 597 787 268 1524
Fragments arranged by size:
1524 1219 787 781 597 393 312 268
EcoO109I rG'GnC_Cy
Cuts at: 2044 2287 2433 2434 2544 2545 2044
Size: 243 146 1 110 1 5380
Fragments arranged by size:
5380 243 146 110 1 1
EcoRI G'AATT_C
Cuts at: 3190 3568 3190
Size: 378 5503
FspI TGC'GCA
Cuts at: 4387 4387
Size: 5881
HaeII r_GCGC'y
Cuts at: 1678 2880 5028 1678
Size: 1202 2148 2531
Fragments arranged by size:
2531 2148 1202
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2558 2590 2648 2680 2996
Size: 32 135 32 986 32 58 32 316
Cuts at: 2996 3028 4522 4554 4596 4628 1373
Size: 32 1494 32 42 32 2626
Fragments arranged by size:
2626 1494 986 316 135 58 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3225 3471 669
Size: 988 281 1287 246 3079
Fragments arranged by size:
3079 1287 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5881
HpaI GTT'AAC
Cuts at: 3471 3471
Size: 5881
KasI G'GCGC_C
Cuts at: 2876 2876
Size: 5881
KpnI G_GTAC'C
Cuts at: 3206 3206
Size: 5881
MfeI C'AATT_G
Cuts at: 3480 3480
Size: 5881
MluI A'CGCG_T
Cuts at: 5757 5757
Size: 5881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4995 5179 1305
Size: 3690 184 2007
Fragments arranged by size:
3690 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4059
Fragments arranged by size:
4059 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2243 2742 3839 4198 4357 518
Size: 648 1077 499 1097 359 159 2042
Fragments arranged by size:
2042 1097 1077 648 499 359 159
MspA1I CmG'CkG
Cuts at: 1606 2310 2340 2359 2523 2599 2632 2964 3297
Size: 704 30 19 164 76 33 332 333
Cuts at: 3297 3974 5122 5367 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 704 677 333 332 245 164
76 33 30 19
NaeI GCC'GGC
Cuts at: 2282 2282
Size: 5881
NarI GG'CG_CC
Cuts at: 2877 2877
Size: 5881
NcoI C'CATG_G
Cuts at: 513 1065 2004 3063 513
Size: 552 939 1059 3331
Fragments arranged by size:
3331 1059 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5881
NgoMIV G'CCGG_C
Cuts at: 2280 2280
Size: 5881
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5881
NotI GC'GGCC_GC
Cuts at: 2968 3328 2968
Size: 360 5521
NruI TCG'CGA
Cuts at: 2802 2802
Size: 5881
NspI r_CATG'y
Cuts at: 1132 1229 3239 4784 1132
Size: 97 2010 1545 2229
Fragments arranged by size:
2229 2010 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3123 1348
Size: 1775 4106
PciI A'CATG_T
Cuts at: 4780 4780
Size: 5881
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3047 1108
Size: 1939 3942
PfoI T'CCnGG_A
Cuts at: 2748 2748
Size: 5881
PmeI GTTT'AAAC
Cuts at: 3185 3185
Size: 5881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3950 3982 5463 5495 3950
Size: 32 1481 32 4336
Fragments arranged by size:
4336 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2757 1613
Size: 1144 4737
PsiI TTA'TAA
Cuts at: 3451 3451
Size: 5881
PspOMI G'GGCC_C
Cuts at: 2287 2381 2433 2506 2544 2626 2287
Size: 94 52 73 38 82 5542
Fragments arranged by size:
5542 94 82 73 52 38
PspXI vC'TCGA_Gb
Cuts at: 2665 3013 2665
Size: 348 5533
PstI C_TGCA'G
Cuts at: 830 3116 3233 830
Size: 2286 117 3478
Fragments arranged by size:
3478 2286 117
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5881
SacII CC_GC'GG
Cuts at: 2311 2341 2360 2633 2311
Size: 30 19 273 5559
Fragments arranged by size:
5559 273 30 19
SalI G'TCGA_C
Cuts at: 1936 3223 1936
Size: 1287 4594
SbfI CC_TGCA'GG
Cuts at: 3233 3233
Size: 5881
ScaI AGT'ACT
Cuts at: 4129 5841 4129
Size: 1712 4169
SfcI C'TryA_G
Cuts at: 826 1045 2773 2959 3112 3229 4364 5045 5236
Size: 219 1728 186 153 117 1135 681 191
Cuts at: 5236 826
Size: 1471
Fragments arranged by size:
1728 1471 1135 681 219 191 186 153
117
SfoI GGC'GCC
Cuts at: 2878 2878
Size: 5881
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5881
SmaI CCC'GGG
Cuts at: 1872 2025 2375 2690 3208 1872
Size: 153 350 315 518 4545
Fragments arranged by size:
4545 518 350 315 153
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2665 2764 3013 3985 4886 5148
Size: 197 926 722 99 249 972 901 262
Cuts at: 5148 820
Size: 1553
Fragments arranged by size:
1553 972 926 901 722 262 249 197
99
SnaBI TAC'GTA
Cuts at: 493 3672 493
Size: 3179 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5881
SphI G_CATG'C
Cuts at: 1132 3239 1132
Size: 2107 3774
SspI AAT'ATT
Cuts at: 5 52 3805 5
Size: 47 3753 2081
Fragments arranged by size:
3753 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5881
StyI C'CwwG_G
Cuts at: 513 1065 2004 2190 3063 3320 513
Size: 552 939 186 873 257 3074
Fragments arranged by size:
3074 939 873 552 257 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2145 2735 2796 3908 3925 4078 4263
Size: 1255 565 590 61 1112 17 153 185
Cuts at: 4263 4708 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1112 590 565 445 185 153
61 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2715 4127 5839
Size: 274 80 33 51 1443 738 1412 1712
Cuts at: 5839 96
Size: 138
Fragments arranged by size:
1712 1443 1412 738 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5841 5873 5841
Size: 32 5849
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5881
XbaI T'CTAG_A
Cuts at: 3217 3217
Size: 5881
XhoI C'TCGA_G
Cuts at: 1943 2665 3013 1943
Size: 722 348 4811
Fragments arranged by size:
4811 722 348
XmaI C'CCGG_G
Cuts at: 1870 2023 2373 2688 3206 1870
Size: 153 350 315 518 4545
Fragments arranged by size:
4545 518 350 315 153
XmnI GAAnn'nnTTC
Cuts at: 2033 4010 2033
Size: 1977 3904
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3689 276
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PspXI PstI PvuI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI
FspAI NsiI PacI PmlI PpuMI PsrI PvuII RsrII
SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI