Restriction map for
pFN21AE0660
(Circular) (Six-base) MAPSORT of: pFN21AE0660.seq Check: 7691 from: 1 to: 5872
pFN21AE0660 5872 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3682 278
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3215 1937
Size: 1278 4594
Acc65I G'GTAC_C
Cuts at: 3193 3193
Size: 5872
AclI AA'CG_TT
Cuts at: 1563 3999 4372 1563
Size: 2436 373 3063
Fragments arranged by size:
3063 2436 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2098 2239 2773 3109 3935 5319 5587 1632
Size: 466 141 534 336 826 1384 268 1917
Fragments arranged by size:
1917 1384 826 534 466 336 268 141
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5675
AflIII A'CryG_T
Cuts at: 1203 4771 5748 1203
Size: 3568 977 1327
Fragments arranged by size:
3568 1327 977
AgeI A'CCGG_T
Cuts at: 4703 4703
Size: 5872
AhdI GACnn_n'nnGTC
Cuts at: 4601 4601
Size: 5872
AleI CACnn'nnGTG
Cuts at: 1166 3002 1166
Size: 1836 4036
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5872
AlwNI CAG_nnn'CTG
Cuts at: 3615 5187 3615
Size: 1572 4300
ApaLI G'TGCA_C
Cuts at: 3929 5085 3929
Size: 1156 4716
ApoI r'AATT_y
Cuts at: 1493 2269 3181 3407 3559 5668 1493
Size: 776 912 226 152 2109 1697
Fragments arranged by size:
2109 1697 912 776 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4426 160
Size: 872 3394 1606
Fragments arranged by size:
3394 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
AvaI C'yCGr_G
Cuts at: 1870 1943 3197 1870
Size: 73 1254 4545
Fragments arranged by size:
4545 1254 73
BamHI G'GATC_C
Cuts at: 2068 3202 3569 2068
Size: 1134 367 4371
Fragments arranged by size:
4371 1134 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3193 4648 618
Size: 325 217 627 1406 1455 1842
Fragments arranged by size:
1842 1455 1406 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3191 1391
Size: 1800 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2154 2329 928
Size: 971 255 175 4471
Fragments arranged by size:
4471 971 255 175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4063 4097 1903
Size: 34 2126 34 3678
Fragments arranged by size:
3678 2126 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3764 4974 3764
Size: 1210 4662
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5872
BfrBI ATG'CAT
Cuts at: 2681 2681
Size: 5872
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4483 136
Size: 107 122 71 631 605 2811 1525
Fragments arranged by size:
2811 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2738 5867 2738
Size: 3129 2743
BlpI GC'TnA_GC
Cuts at: 3289 3289
Size: 5872
Bme1580I G_kGCm'C
Cuts at: 1790 3058 3933 5089 1790
Size: 1268 875 1156 2573
Fragments arranged by size:
2573 1268 1156 875
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5872
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3603 4561 442
Size: 416 53 83 399 24 2186 958 1753
Fragments arranged by size:
2186 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5872
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2319 2601 2685 2769 2853 3021 3105 4532
Size: 1080 282 84 84 84 168 84 1427
Cuts at: 4532 1239
Size: 2579
Fragments arranged by size:
2579 1427 1080 282 168 84 84 84
84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3997 4862 5160 3997
Size: 865 298 4709
Fragments arranged by size:
4709 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4535 882
Size: 617 3036 2219
Fragments arranged by size:
3036 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3663 493
Size: 711 2459 2702
Fragments arranged by size:
2702 2459 711
BsaBI GATnn'nnATC
Cuts at: 5866 5866
Size: 5872
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3679 4061 4696 275
Size: 53 83 186 936 2146 382 635 1451
Fragments arranged by size:
2146 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4305 4703 4977 5124 5535 4305
Size: 398 274 147 411 4642
Fragments arranged by size:
4642 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5647
Fragments arranged by size:
5647 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 1186
Size: 880 4992
BseYI C'CCAG_C
Cuts at: 5075 5571 5075
Size: 496 5376
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5299
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3322 4083 5111 1540
Size: 275 186 1321 761 1028 2301
Fragments arranged by size:
2301 1321 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2573 3933 4018 5089 2573
Size: 1360 85 1071 3356
Fragments arranged by size:
3356 1360 1071 85
BsmI GAATG_Cn'
Cuts at: 2321 3383 3476 2321
Size: 1062 93 4717
Fragments arranged by size:
4717 1062 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2573 3058 3191 3933 4018 5089 1391
Size: 399 783 485 133 742 85 1071 2174
Fragments arranged by size:
2174 1071 783 742 485 399 133 85
BspEI T'CCGG_A
Cuts at: 5535 5535
Size: 5872
BspHI T'CATG_A
Cuts at: 3761 5491 3761
Size: 1730 4142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3227 844
Size: 2383 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3759 1394
Size: 429 1936 3507
Fragments arranged by size:
3507 1936 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2428 4367 4541 65
Size: 1183 1180 1939 174 1396
Fragments arranged by size:
1939 1396 1183 1180 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4516 4703 1700
Size: 110 2706 187 2869
Fragments arranged by size:
2869 2706 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5872
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5872
BssSI C'ACGA_G
Cuts at: 3932 4944 3932
Size: 1012 4860
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5872
BstBI TT'CG_AA
Cuts at: 3061 3145 4676 3061
Size: 84 1531 4257
Fragments arranged by size:
4257 1531 84
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5872
BstXI CCAn_nnnn'nTGG
Cuts at: 2806 2806
Size: 5872
BstYI r'GATC_y
Cuts at: 1972 2068 2738 3202 3569 3954 3971 5423 5509
Size: 96 670 464 367 385 17 1452 86
Cuts at: 5509 5521 5867 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 670 464 385 367 346 96
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4381
Fragments arranged by size:
4381 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5133
Fragments arranged by size:
5133 683 56
BtsI GCAGTG_nn'
Cuts at: 2326 3388 4182 4202 2326
Size: 1062 794 20 3996
Fragments arranged by size:
3996 1062 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5872
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2422 2457 529
Size: 35 1858 35 3944
Fragments arranged by size:
3944 1858 35 35
DraI TTT'AAA
Cuts at: 3176 3176
Size: 5872
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4879 809
Size: 507 3563 1802
Fragments arranged by size:
3563 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3319 4208 5837
Size: 54 1425 50 275 18 1489 889 1629
Cuts at: 5837 8
Size: 43
Fragments arranged by size:
1629 1489 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3319 1537
Size: 275 1507 4090
Fragments arranged by size:
4090 1507 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2727 3802 1353
Size: 1374 1075 3423
Fragments arranged by size:
3423 1374 1075
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4459 4833 4979 387
Size: 1064 138 2870 374 146 1280
Fragments arranged by size:
2870 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2098 2239 2319 2601 2685 2769 2773
Size: 393 466 141 80 282 84 84 4
Cuts at: 2773 2853 3021 3105 3109 3935 4532 5319 5587
Size: 80 168 84 4 826 597 787 268
Cuts at: 5587 1239
Size: 1524
Fragments arranged by size:
1524 826 787 597 466 393 282 268
168 141 84 84 84 80 80 4
4
EcoRI G'AATT_C
Cuts at: 3181 3559 3181
Size: 378 5494
FspI TGC'GCA
Cuts at: 4378 4378
Size: 5872
HaeII r_GCGC'y
Cuts at: 1678 5019 1678
Size: 3341 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 4513 4545 4587
Size: 32 135 32 428 32 2481 32 42
Cuts at: 4587 4619 1373
Size: 32 2626
Fragments arranged by size:
2626 2481 428 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2473 3216 3462 669
Size: 988 281 535 743 246 3079
Fragments arranged by size:
3079 988 743 535 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5872
HpaI GTT'AAC
Cuts at: 3462 3462
Size: 5872
KpnI G_GTAC'C
Cuts at: 3197 3197
Size: 5872
MfeI C'AATT_G
Cuts at: 3471 3471
Size: 5872
MluI A'CGCG_T
Cuts at: 5748 5748
Size: 5872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4986 5170 1305
Size: 3681 184 2007
Fragments arranged by size:
3681 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4050
Fragments arranged by size:
4050 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2314 2596 3002 3830 4189 4348 518
Size: 648 1148 282 406 828 359 159 2042
Fragments arranged by size:
2042 1148 828 648 406 359 282 159
MspA1I CmG'CkG
Cuts at: 1606 3288 3965 5113 5358 1606
Size: 1682 677 1148 245 2120
Fragments arranged by size:
2120 1682 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4381
Fragments arranged by size:
4381 939 552
NdeI CA'TA_TG
Cuts at: 387 2342 387
Size: 1955 3917
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5872
NotI GC'GGCC_GC
Cuts at: 3319 3319
Size: 5872
NsiI A_TGCA'T
Cuts at: 2683 2683
Size: 5872
NspI r_CATG'y
Cuts at: 1132 1229 2685 3230 4775 1132
Size: 97 1456 545 1545 2229
Fragments arranged by size:
2229 1545 1456 545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5872
PciI A'CATG_T
Cuts at: 4771 4771
Size: 5872
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5872
PmeI GTTT'AAAC
Cuts at: 3176 3176
Size: 5872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3941 3973 5454 5486 3941
Size: 32 1481 32 4327
Fragments arranged by size:
4327 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5872
PsiI TTA'TAA
Cuts at: 2511 3442 2511
Size: 931 4941
PstI C_TGCA'G
Cuts at: 830 3224 830
Size: 2394 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5872
SalI G'TCGA_C
Cuts at: 1936 3214 1936
Size: 1278 4594
SbfI CC_TGCA'GG
Cuts at: 3224 3224
Size: 5872
ScaI AGT'ACT
Cuts at: 4120 5832 4120
Size: 1712 4160
SfcI C'TryA_G
Cuts at: 826 1045 2089 2130 3220 4355 5036 5227 826
Size: 219 1044 41 1090 1135 681 191 1471
Fragments arranged by size:
1471 1135 1090 1044 681 219 191 41
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5872
SmaI CCC'GGG
Cuts at: 1872 3199 1872
Size: 1327 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3976 4877 5139 820
Size: 197 926 2033 901 262 1553
Fragments arranged by size:
2033 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3663 493
Size: 3170 2702
SpeI A'CTAG_T
Cuts at: 152 2495 152
Size: 2343 3529
SphI G_CATG'C
Cuts at: 1132 3230 1132
Size: 2098 3774
SspI AAT'ATT
Cuts at: 5 52 2963 3796 5
Size: 47 2911 833 2081
Fragments arranged by size:
2911 2081 833 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5872
StyI C'CwwG_G
Cuts at: 513 1065 2004 3311 513
Size: 552 939 1307 3074
Fragments arranged by size:
3074 1307 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3899 3916 4069 4254 4699 325
Size: 1255 2319 17 153 185 445 1498
Fragments arranged by size:
2319 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4118 5830 96
Size: 274 80 33 51 1443 2141 1712 138
Fragments arranged by size:
2141 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2899 2931 5832 5864 2899
Size: 32 2901 32 2907
Fragments arranged by size:
2907 2901 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5872
XbaI T'CTAG_A
Cuts at: 3208 3208
Size: 5872
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5872
XmaI C'CCGG_G
Cuts at: 1870 3197 1870
Size: 1327 4545
XmnI GAAnn'nnTTC
Cuts at: 4001 4001
Size: 5872
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3680 276
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI