Restriction map for
pFN21AE0657
(Circular) (Six-base) MAPSORT of: pFN21AE0657.seq Check: 7343 from: 1 to: 5404
pFN21AE0657 5404 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3214 278
Size: 53 83 186 936 1678 2468
Fragments arranged by size:
2468 1678 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2747 1937
Size: 810 4594
Acc65I G'GTAC_C
Cuts at: 2725 2725
Size: 5404
AclI AA'CG_TT
Cuts at: 1563 3531 3904 1563
Size: 1968 373 3063
Fragments arranged by size:
3063 1968 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2030 2159 3467 4851 5119 1632
Size: 398 129 1308 1384 268 1917
Fragments arranged by size:
1917 1384 1308 398 268 129
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5207
AflIII A'CryG_T
Cuts at: 1203 2697 4303 5280 1203
Size: 1494 1606 977 1327
Fragments arranged by size:
1606 1494 1327 977
AgeI A'CCGG_T
Cuts at: 4235 4235
Size: 5404
AhdI GACnn_n'nnGTC
Cuts at: 4133 4133
Size: 5404
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5404
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5404
AlwNI CAG_nnn'CTG
Cuts at: 3147 4719 3147
Size: 1572 3832
ApaLI G'TGCA_C
Cuts at: 2144 3461 4617 2144
Size: 1317 1156 2931
Fragments arranged by size:
2931 1317 1156
ApoI r'AATT_y
Cuts at: 1493 2252 2389 2713 2939 3091 5200 1493
Size: 759 137 324 226 152 2109 1697
Fragments arranged by size:
2109 1697 759 324 226 152 137
AseI AT'TA_AT
Cuts at: 160 1032 3958 160
Size: 872 2926 1606
Fragments arranged by size:
2926 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5404
AvaI C'yCGr_G
Cuts at: 1870 1943 2322 2729 1870
Size: 73 379 407 4545
Fragments arranged by size:
4545 407 379 73
BamHI G'GATC_C
Cuts at: 2734 3101 2734
Size: 367 5037
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2725 4180 618
Size: 325 217 627 938 1455 1842
Fragments arranged by size:
1842 1455 938 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2723 1391
Size: 1332 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4433
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2240 2274 3595 3629 1903
Size: 34 50 34 219 34 1321 34 3678
Fragments arranged by size:
3678 1321 219 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3296 4506 3296
Size: 1210 4194
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5404
BfrBI ATG'CAT
Cuts at: 2221 2221
Size: 5404
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4015 136
Size: 107 122 71 631 605 2343 1525
Fragments arranged by size:
2343 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5399 5399
Size: 5404
BlpI GC'TnA_GC
Cuts at: 2821 2821
Size: 5404
Bme1580I G_kGCm'C
Cuts at: 1790 2148 3465 4621 1790
Size: 358 1317 1156 2573
Fragments arranged by size:
2573 1317 1156 358
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5404
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3135 4093 442
Size: 416 53 83 399 24 1718 958 1753
Fragments arranged by size:
1753 1718 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5404
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2151 2442 4064 1239
Size: 912 291 1622 2579
Fragments arranged by size:
2579 1622 912 291
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2513 3529 4394 4692 2513
Size: 1016 865 298 3225
Fragments arranged by size:
3225 1016 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2454 4067 882
Size: 617 955 1613 2219
Fragments arranged by size:
2219 1613 955 617
BsaAI yAC'GTr
Cuts at: 493 1204 3195 493
Size: 711 1991 2702
Fragments arranged by size:
2702 1991 711
BsaBI GATnn'nnATC
Cuts at: 5398 5398
Size: 5404
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2626 3211 3593 4228
Size: 53 83 186 936 1093 585 382 635
Cuts at: 4228 275
Size: 1451
Fragments arranged by size:
1451 1093 936 635 585 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3837 4235 4509 4656 5067 3837
Size: 398 274 147 411 4174
Fragments arranged by size:
4174 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2014 2044 2475 2505 1178
Size: 30 165 30 611 30 431 30 4077
Fragments arranged by size:
4077 611 431 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2472 1186
Size: 1286 4118
BseYI C'CCAG_C
Cuts at: 4607 5103 4607
Size: 496 4908
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4831
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2854 3615 4643 1540
Size: 275 186 853 761 1028 2301
Fragments arranged by size:
2301 1028 853 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2148 3465 3550 4621 2148
Size: 1317 85 1071 2931
Fragments arranged by size:
2931 1317 1071 85
BsmI GAATG_Cn'
Cuts at: 2915 3008 2915
Size: 93 5311
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2723 3465 3550 4621 1391
Size: 399 358 575 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 575 399 358 85
BspEI T'CCGG_A
Cuts at: 5067 5067
Size: 5404
BspHI T'CATG_A
Cuts at: 3293 5023 3293
Size: 1730 3674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2759 844
Size: 1915 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2071 3291 1394
Size: 429 248 1220 3507
Fragments arranged by size:
3507 1220 429 248
BsrDI GCAATG_nn'
Cuts at: 65 1248 3899 4073 65
Size: 1183 2651 174 1396
Fragments arranged by size:
2651 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4048 4235 1700
Size: 110 2238 187 2869
Fragments arranged by size:
2869 2238 187 110
BsrGI T'GTAC_A
Cuts at: 96 2189 96
Size: 2093 3311
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5404
BssSI C'ACGA_G
Cuts at: 3464 4476 3464
Size: 1012 4392
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2285 2294 2644 794
Size: 1491 9 350 3554
Fragments arranged by size:
3554 1491 350 9
BstBI TT'CG_AA
Cuts at: 4208 4208
Size: 5404
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5404
BstYI r'GATC_y
Cuts at: 1972 2734 3101 3486 3503 4955 5041 5053 5399
Size: 762 367 385 17 1452 86 12 346
Cuts at: 5399 1972
Size: 1977
Fragments arranged by size:
1977 1452 762 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2034 513
Size: 552 969 3883
Fragments arranged by size:
3883 969 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4665
Fragments arranged by size:
4665 683 56
BtsI GCAGTG_nn'
Cuts at: 2920 3714 3734 2920
Size: 794 20 4590
Fragments arranged by size:
4590 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5404
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5369
DraI TTT'AAA
Cuts at: 2708 2708
Size: 5404
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4411 809
Size: 507 3095 1802
Fragments arranged by size:
3095 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2851 3740 5369
Size: 54 1425 50 275 18 1021 889 1629
Cuts at: 5369 8
Size: 43
Fragments arranged by size:
1629 1425 1021 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2851 1537
Size: 275 1039 4090
Fragments arranged by size:
4090 1039 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3334 1353
Size: 1981 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3991 4365 4511 387
Size: 1064 138 2402 374 146 1280
Fragments arranged by size:
2402 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2151 2159 2442 3467 4064 4851
Size: 393 398 121 8 283 1025 597 787
Cuts at: 4851 5119 1239
Size: 268 1524
Fragments arranged by size:
1524 1025 787 597 398 393 283 268
121 8
EcoRI G'AATT_C
Cuts at: 2252 2713 3091 2252
Size: 461 378 4565
Fragments arranged by size:
4565 461 378
FspI TGC'GCA
Cuts at: 3910 3910
Size: 5404
HaeII r_GCGC'y
Cuts at: 1678 4551 1678
Size: 2873 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4045 4077 4119 4151 1373
Size: 32 135 32 2473 32 42 32 2626
Fragments arranged by size:
2626 2473 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2748 2994 669
Size: 988 281 810 246 3079
Fragments arranged by size:
3079 988 810 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5404
HpaI GTT'AAC
Cuts at: 2994 2994
Size: 5404
KpnI G_GTAC'C
Cuts at: 2729 2729
Size: 5404
MfeI C'AATT_G
Cuts at: 2100 2642 3003 2100
Size: 542 361 4501
Fragments arranged by size:
4501 542 361
MluI A'CGCG_T
Cuts at: 5280 5280
Size: 5404
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4518 4702 1305
Size: 3213 184 2007
Fragments arranged by size:
3213 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3582
Fragments arranged by size:
3582 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3362 3721 3880 518
Size: 648 2196 359 159 2042
Fragments arranged by size:
2196 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2467 2820 3497 4645 4890 1606
Size: 861 353 677 1148 245 2120
Fragments arranged by size:
2120 1148 861 677 353 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4852
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5404
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5404
NotI GC'GGCC_GC
Cuts at: 2851 2851
Size: 5404
NsiI A_TGCA'T
Cuts at: 2223 2223
Size: 5404
NspI r_CATG'y
Cuts at: 1132 1229 2701 2762 4307 1132
Size: 97 1472 61 1545 2229
Fragments arranged by size:
2229 1545 1472 97 61
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5404
PciI A'CATG_T
Cuts at: 2697 4303 2697
Size: 1606 3798
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5404
PfoI T'CCnGG_A
Cuts at: 2419 2419
Size: 5404
PmeI GTTT'AAAC
Cuts at: 2708 2708
Size: 5404
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3505 4986 5018 3473
Size: 32 1481 32 3859
Fragments arranged by size:
3859 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2060 1613
Size: 447 4957
PsiI TTA'TAA
Cuts at: 2974 2974
Size: 5404
PspXI vC'TCGA_Gb
Cuts at: 2322 2322
Size: 5404
PstI C_TGCA'G
Cuts at: 830 2756 830
Size: 1926 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5404
PvuII CAG'CTG
Cuts at: 2467 2467
Size: 5404
SalI G'TCGA_C
Cuts at: 1936 2746 1936
Size: 810 4594
SbfI CC_TGCA'GG
Cuts at: 2756 2756
Size: 5404
ScaI AGT'ACT
Cuts at: 3652 5364 3652
Size: 1712 3692
SfcI C'TryA_G
Cuts at: 826 1045 2120 2752 3887 4568 4759 826
Size: 219 1075 632 1135 681 191 1471
Fragments arranged by size:
1471 1135 1075 681 632 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5404
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5404
SmaI CCC'GGG
Cuts at: 1872 2731 1872
Size: 859 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2322 2528 3508 4409 4671 820
Size: 197 926 379 206 980 901 262 1553
Fragments arranged by size:
1553 980 926 901 379 262 206 197
SnaBI TAC'GTA
Cuts at: 493 3195 493
Size: 2702 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5404
SphI G_CATG'C
Cuts at: 1132 2762 1132
Size: 1630 3774
SspI AAT'ATT
Cuts at: 5 52 3328 5
Size: 47 3276 2081
Fragments arranged by size:
3276 2081 47
StuI AGG'CCT
Cuts at: 1517 2355 2448 1517
Size: 838 93 4473
Fragments arranged by size:
4473 838 93
StyI C'CwwG_G
Cuts at: 513 1065 2356 2843 513
Size: 552 1291 487 3074
Fragments arranged by size:
3074 1291 552 487
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3431 3448 3601 3786 4231 325
Size: 1255 1851 17 153 185 445 1498
Fragments arranged by size:
1851 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2189 3650 5362
Size: 274 80 33 51 1443 212 1461 1712
Cuts at: 5362 96
Size: 138
Fragments arranged by size:
1712 1461 1443 274 212 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2014 2046 2475 2507 5364 5396 2014
Size: 32 429 32 2857 32 2022
Fragments arranged by size:
2857 2022 429 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2546 1316
Size: 1230 4174
XbaI T'CTAG_A
Cuts at: 2740 2740
Size: 5404
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2041 2041
Size: 5404
XhoI C'TCGA_G
Cuts at: 1943 2322 1943
Size: 379 5025
XmaI C'CCGG_G
Cuts at: 1870 2729 1870
Size: 859 4545
XmnI GAAnn'nnTTC
Cuts at: 3533 3533
Size: 5404
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3212 276
Size: 53 83 186 936 1678 2468
Fragments arranged by size:
2468 1678 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI
PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI