Restriction map for
pFN21AE0656
(Circular) (Six-base) MAPSORT of: pFN21AE0656.seq Check: 1388 from: 1 to: 5869
pFN21AE0656 5869 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2949 2949
Size: 5869
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3679 278
Size: 53 83 186 936 2143 2468
Fragments arranged by size:
2468 2143 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3212 1937
Size: 1275 4594
Acc65I G'GTAC_C
Cuts at: 3190 3190
Size: 5869
AclI AA'CG_TT
Cuts at: 1563 3996 4369 1563
Size: 2433 373 3063
Fragments arranged by size:
3063 2433 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2414 2708 2804 2841 3932 5316 5584 1632
Size: 782 294 96 37 1091 1384 268 1917
Fragments arranged by size:
1917 1384 1091 782 294 268 96 37
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5672
AflIII A'CryG_T
Cuts at: 1203 2037 2039 2859 4768 5745 1203
Size: 834 2 820 1909 977 1327
Fragments arranged by size:
1909 1327 977 834 820 2
AgeI A'CCGG_T
Cuts at: 4700 4700
Size: 5869
AhdI GACnn_n'nnGTC
Cuts at: 4598 4598
Size: 5869
AleI CACnn'nnGTG
Cuts at: 1166 2370 1166
Size: 1204 4665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5869
AlwNI CAG_nnn'CTG
Cuts at: 3612 5184 3612
Size: 1572 4297
ApaLI G'TGCA_C
Cuts at: 3926 5082 3926
Size: 1156 4713
ApoI r'AATT_y
Cuts at: 1493 2030 2125 2807 2981 3178 3404 3556 5665
Size: 537 95 682 174 197 226 152 2109
Cuts at: 5665 1493
Size: 1697
Fragments arranged by size:
2109 1697 682 537 226 197 174 152
95
AseI AT'TA_AT
Cuts at: 160 1032 4423 160
Size: 872 3391 1606
Fragments arranged by size:
3391 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5869
AvaI C'yCGr_G
Cuts at: 1870 1943 3194 1870
Size: 73 1251 4545
Fragments arranged by size:
4545 1251 73
AvrII C'CTAG_G
Cuts at: 2338 2338
Size: 5869
BamHI G'GATC_C
Cuts at: 3199 3566 3199
Size: 367 5502
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3190 4645 618
Size: 325 217 627 1403 1455 1842
Fragments arranged by size:
1842 1455 1403 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3188 1391
Size: 1797 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4898
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4060 4094 1903
Size: 34 2123 34 3678
Fragments arranged by size:
3678 2123 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3761 4971 3761
Size: 1210 4659
BclI T'GATC_A
Cuts at: 1548 2269 1548
Size: 721 5148
BfrBI ATG'CAT
Cuts at: 2659 2659
Size: 5869
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4480 136
Size: 107 122 71 631 605 2808 1525
Fragments arranged by size:
2808 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2147 5864 2147
Size: 3717 2152
BlpI GC'TnA_GC
Cuts at: 2724 3286 2724
Size: 562 5307
Bme1580I G_kGCm'C
Cuts at: 1790 3930 5086 1790
Size: 2140 1156 2573
Fragments arranged by size:
2573 2140 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5869
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3600 4558 442
Size: 416 53 83 399 24 2183 958 1753
Fragments arranged by size:
2183 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5869
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3034 3034
Size: 5869
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 3091 4529 1239
Size: 842 1010 1438 2579
Fragments arranged by size:
2579 1438 1010 842
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3056 3994 4859 5157 3056
Size: 938 865 298 3768
Fragments arranged by size:
3768 938 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4532 882
Size: 617 3033 2219
Fragments arranged by size:
3033 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2040 3660 493
Size: 711 836 1620 2702
Fragments arranged by size:
2702 1620 836 711
BsaBI GATnn'nnATC
Cuts at: 5863 5863
Size: 5869
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3676 4058 4693 275
Size: 53 83 186 936 2143 382 635 1451
Fragments arranged by size:
2143 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4302 4700 4974 5121 5532 4302
Size: 398 274 147 411 4639
Fragments arranged by size:
4639 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2162 2192 1178
Size: 30 165 30 759 30 4855
Fragments arranged by size:
4855 759 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2244 2358 2390 2456 3014 1186
Size: 1058 114 32 66 558 4041
Fragments arranged by size:
4041 1058 558 114 66 32
BseYI C'CCAG_C
Cuts at: 5072 5568 5072
Size: 496 5373
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2104 1163
Size: 573 368 4928
Fragments arranged by size:
4928 573 368
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3319 4080 5108 1540
Size: 275 186 1318 761 1028 2301
Fragments arranged by size:
2301 1318 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3930 4015 5086 3930
Size: 85 1071 4713
Fragments arranged by size:
4713 1071 85
BsmI GAATG_Cn'
Cuts at: 3380 3473 3380
Size: 93 5776
BsmBI CGTCTCn'nnnn_
Cuts at: 2372 2927 2372
Size: 555 5314
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3188 3930 4015 5086 1391
Size: 399 1398 742 85 1071 2174
Fragments arranged by size:
2174 1398 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5532 5532
Size: 5869
BspHI T'CATG_A
Cuts at: 2466 2625 3758 5488 2466
Size: 159 1133 1730 2847
Fragments arranged by size:
2847 1730 1133 159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2949 3224 844
Size: 2105 275 3489
Fragments arranged by size:
3489 2105 275
BsrBI CCG'CTC
Cuts at: 1394 1823 2300 3756 1394
Size: 429 477 1456 3507
Fragments arranged by size:
3507 1456 477 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4364 4538 65
Size: 1183 3116 174 1396
Fragments arranged by size:
3116 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4513 4700 1700
Size: 110 2703 187 2869
Fragments arranged by size:
2869 2703 187 110
BsrGI T'GTAC_A
Cuts at: 96 2667 96
Size: 2571 3298
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5869
BssSI C'ACGA_G
Cuts at: 3929 4941 3929
Size: 1012 4857
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5869
BstBI TT'CG_AA
Cuts at: 4673 4673
Size: 5869
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5869
BstYI r'GATC_y
Cuts at: 1972 2147 3199 3566 3951 3968 5420 5506 5518
Size: 175 1052 367 385 17 1452 86 12
Cuts at: 5518 5864 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1052 385 367 346 175 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5317
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2863 487
Size: 683 56 1637 3493
Fragments arranged by size:
3493 1637 683 56
BtsI GCAGTG_nn'
Cuts at: 3385 4179 4199 3385
Size: 794 20 5055
Fragments arranged by size:
5055 794 20
ClaI AT'CG_AT
Cuts at: 1554 2583 1554
Size: 1029 4840
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5834
DraI TTT'AAA
Cuts at: 3173 3173
Size: 5869
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4876 809
Size: 507 3560 1802
Fragments arranged by size:
3560 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2356 2382 2754
Size: 54 1425 50 275 18 526 26 372
Cuts at: 2754 3316 4205 5834 8
Size: 562 889 1629 43
Fragments arranged by size:
1629 1425 889 562 526 372 275 54
50 43 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 3316 1537
Size: 275 1504 4090
Fragments arranged by size:
4090 1504 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2012 2085 3035 3799 1353
Size: 659 73 950 764 3423
Fragments arranged by size:
3423 950 764 659 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4456 4830 4976 387
Size: 1064 138 2867 374 146 1280
Fragments arranged by size:
2867 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2414 2708 2804 2841 3091 3932
Size: 393 449 333 294 96 37 250 841
Cuts at: 3932 4529 5316 5584 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 841 787 597 449 393 333 294
268 250 96 37
EcoRI G'AATT_C
Cuts at: 2030 3178 3556 2030
Size: 1148 378 4343
Fragments arranged by size:
4343 1148 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2809 2809
Size: 5869
FspI TGC'GCA
Cuts at: 4375 4375
Size: 5869
HaeII r_GCGC'y
Cuts at: 1678 5016 1678
Size: 3338 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2132 2162 2164 2194 4510
Size: 32 135 32 560 30 2 30 2316
Cuts at: 4510 4542 4584 4616 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2316 560 135 42 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2242 2305 3213 3459 669
Size: 988 281 304 63 908 246 3079
Fragments arranged by size:
3079 988 908 304 281 246 63
HindIII A'AGCT_T
Cuts at: 748 3023 748
Size: 2275 3594
HpaI GTT'AAC
Cuts at: 3459 3459
Size: 5869
KpnI G_GTAC'C
Cuts at: 3194 3194
Size: 5869
MfeI C'AATT_G
Cuts at: 3468 3468
Size: 5869
MluI A'CGCG_T
Cuts at: 5745 5745
Size: 5869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4983 5167 1305
Size: 3678 184 2007
Fragments arranged by size:
3678 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2756 10
Size: 54 1425 343 924 3123
Fragments arranged by size:
3123 1425 924 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2370 2858 3827 4186 4345 518
Size: 648 1204 488 969 359 159 2042
Fragments arranged by size:
2042 1204 969 648 488 359 159
MspA1I CmG'CkG
Cuts at: 1606 2059 2476 2500 3285 3962 5110 5355 1606
Size: 453 417 24 785 677 1148 245 2120
Fragments arranged by size:
2120 1148 785 677 453 417 245 24
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5317
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5869
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5869
NotI GC'GGCC_GC
Cuts at: 3316 3316
Size: 5869
NsiI A_TGCA'T
Cuts at: 2661 2661
Size: 5869
NspI r_CATG'y
Cuts at: 1132 1229 2659 2863 3227 4772 1132
Size: 97 1430 204 364 1545 2229
Fragments arranged by size:
2229 1545 1430 364 204 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5869
PciI A'CATG_T
Cuts at: 2859 4768 2859
Size: 1909 3960
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5869
PmeI GTTT'AAAC
Cuts at: 3173 3173
Size: 5869
PmlI CAC'GTG
Cuts at: 2040 2040
Size: 5869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3938 3970 5451 5483 3938
Size: 32 1481 32 4324
Fragments arranged by size:
4324 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5869
PsiI TTA'TAA
Cuts at: 2046 3439 2046
Size: 1393 4476
PstI C_TGCA'G
Cuts at: 830 2960 3221 830
Size: 2130 261 3478
Fragments arranged by size:
3478 2130 261
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5869
PvuII CAG'CTG
Cuts at: 2059 2476 2500 2059
Size: 417 24 5428
Fragments arranged by size:
5428 417 24
SalI G'TCGA_C
Cuts at: 1936 3211 1936
Size: 1275 4594
SbfI CC_TGCA'GG
Cuts at: 2960 3221 2960
Size: 261 5608
ScaI AGT'ACT
Cuts at: 4117 5829 4117
Size: 1712 4157
SfcI C'TryA_G
Cuts at: 826 1045 2956 3217 4352 5033 5224 826
Size: 219 1911 261 1135 681 191 1471
Fragments arranged by size:
1911 1471 1135 681 261 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5869
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5869
SmaI CCC'GGG
Cuts at: 1872 3196 1872
Size: 1324 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3071 3973 4874 5136 820
Size: 197 926 1128 902 901 262 1553
Fragments arranged by size:
1553 1128 926 902 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3660 493
Size: 3167 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5869
SphI G_CATG'C
Cuts at: 1132 3227 1132
Size: 2095 3774
SspI AAT'ATT
Cuts at: 5 52 3793 5
Size: 47 3741 2081
Fragments arranged by size:
3741 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5869
StyI C'CwwG_G
Cuts at: 513 1065 2338 3006 3308 513
Size: 552 1273 668 302 3074
Fragments arranged by size:
3074 1273 668 552 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2906 3896 3913 4066 4251 4696 325
Size: 1255 1326 990 17 153 185 445 1498
Fragments arranged by size:
1498 1326 1255 990 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2667 4115 5827
Size: 274 80 33 51 1443 690 1448 1712
Cuts at: 5827 96
Size: 138
Fragments arranged by size:
1712 1448 1443 690 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5829 5861 5829
Size: 32 5837
Tth111I GACn'n_nGTC
Cuts at: 1316 2531 1316
Size: 1215 4654
XbaI T'CTAG_A
Cuts at: 3205 3205
Size: 5869
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5869
XmaI C'CCGG_G
Cuts at: 1870 3194 1870
Size: 1324 4545
XmnI GAAnn'nnTTC
Cuts at: 2982 3998 2982
Size: 1016 4853
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3677 276
Size: 53 83 186 936 2143 2468
Fragments arranged by size:
2468 2143 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI