Restriction map for
pFN21AE0652
(Circular) (Six-base) MAPSORT of: pFN21AE0652.seq Check: 2355 from: 1 to: 5860
pFN21AE0652 5860 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3670 278
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3203 1937
Size: 1266 4594
Acc65I G'GTAC_C
Cuts at: 3181 3181
Size: 5860
AclI AA'CG_TT
Cuts at: 1563 3987 4360 1563
Size: 2424 373 3063
Fragments arranged by size:
3063 2424 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2073 2145 2201 2595 3923 5307 5575 1632
Size: 441 72 56 394 1328 1384 268 1917
Fragments arranged by size:
1917 1384 1328 441 394 268 72 56
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5663
AflIII A'CryG_T
Cuts at: 1203 2808 2810 4759 5736 1203
Size: 1605 2 1949 977 1327
Fragments arranged by size:
1949 1605 1327 977 2
AgeI A'CCGG_T
Cuts at: 4691 4691
Size: 5860
AhdI GACnn_n'nnGTC
Cuts at: 2617 4589 2617
Size: 1972 3888
AleI CACnn'nnGTG
Cuts at: 1166 3131 1166
Size: 1965 3895
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5860
AlwNI CAG_nnn'CTG
Cuts at: 3603 5175 3603
Size: 1572 4288
ApaLI G'TGCA_C
Cuts at: 3143 3917 5073 3143
Size: 774 1156 3930
Fragments arranged by size:
3930 1156 774
ApoI r'AATT_y
Cuts at: 1493 3169 3395 3547 5656 1493
Size: 1676 226 152 2109 1697
Fragments arranged by size:
2109 1697 1676 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4414 160
Size: 872 3382 1606
Fragments arranged by size:
3382 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 3185 1870
Size: 73 67 1175 4545
Fragments arranged by size:
4545 1175 73 67
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2797 2830 2797
Size: 33 5827
BamHI G'GATC_C
Cuts at: 3190 3557 3190
Size: 367 5493
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2829 3049 3181 4636 618
Size: 325 217 627 1042 220 132 1455 1842
Fragments arranged by size:
1842 1455 1042 627 325 220 217 132
BanII G_rGCy'C
Cuts at: 1391 3179 1391
Size: 1788 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2061 2265 928
Size: 971 162 204 4523
Fragments arranged by size:
4523 971 204 162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2807 2841 2991 3025 4051 4085 1903
Size: 34 870 34 150 34 1026 34 3678
Fragments arranged by size:
3678 1026 870 150 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2174 3752 4962 2174
Size: 1578 1210 3072
Fragments arranged by size:
3072 1578 1210
BclI T'GATC_A
Cuts at: 1548 2490 1548
Size: 942 4918
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4471 136
Size: 107 122 71 631 605 2799 1525
Fragments arranged by size:
2799 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2067 5855 2067
Size: 3788 2072
BlpI GC'TnA_GC
Cuts at: 3277 3277
Size: 5860
Bme1580I G_kGCm'C
Cuts at: 1790 3147 3921 5077 1790
Size: 1357 774 1156 2573
Fragments arranged by size:
2573 1357 1156 774
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5860
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2746 3591 4549
Size: 416 53 83 399 24 1329 845 958
Cuts at: 4549 442
Size: 1753
Fragments arranged by size:
1753 1329 958 845 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5860
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2047 2743 2047
Size: 696 5164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2541 4520 1239
Size: 1302 1979 2579
Fragments arranged by size:
2579 1979 1302
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2423 2690 3985 4850 5148 2423
Size: 267 1295 865 298 3135
Fragments arranged by size:
3135 1295 865 298 267
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2078 2427 3097 3109 4523 882
Size: 617 579 349 670 12 1414 2219
Fragments arranged by size:
2219 1414 670 617 579 349 12
BsaAI yAC'GTr
Cuts at: 493 1204 2811 3651 493
Size: 711 1607 840 2702
Fragments arranged by size:
2702 1607 840 711
BsaBI GATnn'nnATC
Cuts at: 5854 5854
Size: 5860
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2394 3667 4049 4684
Size: 53 83 186 936 861 1273 382 635
Cuts at: 4684 275
Size: 1451
Fragments arranged by size:
1451 1273 936 861 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2309 2328 2895 4293 4691 4965 5112 5523 2309
Size: 19 567 1398 398 274 147 411 2646
Fragments arranged by size:
2646 1398 567 411 398 274 147 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2330 2360 1178
Size: 30 165 30 927 30 4678
Fragments arranged by size:
4678 927 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 2489 1186
Size: 837 466 4557
Fragments arranged by size:
4557 837 466
BseYI C'CCAG_C
Cuts at: 5063 5559 5063
Size: 496 5364
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5287
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2946 3310 4071 5099 1540
Size: 275 186 945 364 761 1028 2301
Fragments arranged by size:
2301 1028 945 761 364 275 186
BsiHKAI G_wGCw'C
Cuts at: 3147 3921 4006 5077 3147
Size: 774 85 1071 3930
Fragments arranged by size:
3930 1071 774 85
BsmI GAATG_Cn'
Cuts at: 3371 3464 3371
Size: 93 5767
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3147 3179 3921 4006 5077 1391
Size: 399 1357 32 742 85 1071 2174
Fragments arranged by size:
2174 1357 1071 742 399 85 32
BspEI T'CCGG_A
Cuts at: 2895 5523 2895
Size: 2628 3232
BspHI T'CATG_A
Cuts at: 3749 5479 3749
Size: 1730 4130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3215 844
Size: 2371 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3112 3747 1394
Size: 429 1289 635 3507
Fragments arranged by size:
3507 1289 635 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4355 4529 65
Size: 1183 3107 174 1396
Fragments arranged by size:
3107 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4504 4691 1700
Size: 110 2694 187 2869
Fragments arranged by size:
2869 2694 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5860
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5860
BssSI C'ACGA_G
Cuts at: 2873 3920 4932 2873
Size: 1047 1012 3801
Fragments arranged by size:
3801 1047 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2936 794
Size: 2142 3718
BstBI TT'CG_AA
Cuts at: 4664 4664
Size: 5860
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5860
BstYI r'GATC_y
Cuts at: 1972 2067 2700 2892 3190 3557 3942 3959 5411
Size: 95 633 192 298 367 385 17 1452
Cuts at: 5411 5497 5509 5855 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 633 385 367 346 298 192
95 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2834 2940 513
Size: 552 939 830 106 3433
Fragments arranged by size:
3433 939 830 552 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5121
Fragments arranged by size:
5121 683 56
BtsI GCAGTG_nn'
Cuts at: 2541 3147 3376 4170 4190 2541
Size: 606 229 794 20 4211
Fragments arranged by size:
4211 794 606 229 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5825
DraI TTT'AAA
Cuts at: 3164 3164
Size: 5860
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4867 809
Size: 507 3551 1802
Fragments arranged by size:
3551 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2943 3027 3307
Size: 54 1425 50 275 18 1113 84 280
Cuts at: 3307 4196 5825 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1113 889 280 275 84 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2943 3307 1537
Size: 275 1131 364 4090
Fragments arranged by size:
4090 1131 364 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2121 3790 1353
Size: 768 1669 3423
Fragments arranged by size:
3423 1669 768
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2369 2726 4447 4821 4967 387
Size: 1064 138 780 357 1721 374 146 1280
Fragments arranged by size:
1721 1280 1064 780 374 357 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2073 2145 2201 2541 2595 3923 4520
Size: 393 441 72 56 340 54 1328 597
Cuts at: 4520 5307 5575 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1328 787 597 441 393 340 268
72 56 54
EcoO109I rG'GnC_Cy
Cuts at: 2100 2791 2100
Size: 691 5169
EcoRI G'AATT_C
Cuts at: 3169 3547 3169
Size: 378 5482
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 5860
FspI TGC'GCA
Cuts at: 4366 4366
Size: 5860
HaeII r_GCGC'y
Cuts at: 1678 2180 2889 5007 1678
Size: 502 709 2118 2531
Fragments arranged by size:
2531 2118 709 502
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2462 2494 2522 2554 2603
Size: 32 135 32 890 32 28 32 49
Cuts at: 2603 2635 2732 2764 4501 4533 4575 4607 1373
Size: 32 97 32 1737 32 42 32 2626
Fragments arranged by size:
2626 1737 890 135 97 49 42 32
32 32 32 32 32 32 32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 2611 3204 3450 669
Size: 988 281 673 593 246 3079
Fragments arranged by size:
3079 988 673 593 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5860
HpaI GTT'AAC
Cuts at: 3450 3450
Size: 5860
KpnI G_GTAC'C
Cuts at: 3185 3185
Size: 5860
MfeI C'AATT_G
Cuts at: 3459 3459
Size: 5860
MluI A'CGCG_T
Cuts at: 5736 5736
Size: 5860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2775 3082 4974 5158 1305
Size: 1470 307 1892 184 2007
Fragments arranged by size:
2007 1892 1470 307 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3029 10
Size: 54 1425 343 1197 2841
Fragments arranged by size:
2841 1425 1197 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2627 3131 3818 4177 4336 518
Size: 648 1461 504 687 359 159 2042
Fragments arranged by size:
2042 1461 687 648 504 359 159
MspA1I CmG'CkG
Cuts at: 1606 2302 3276 3953 5101 5346 1606
Size: 696 974 677 1148 245 2120
Fragments arranged by size:
2120 1148 974 696 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2834 513
Size: 552 939 830 3539
Fragments arranged by size:
3539 939 830 552
NdeI CA'TA_TG
Cuts at: 387 2958 387
Size: 2571 3289
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5860
NotI GC'GGCC_GC
Cuts at: 3307 3307
Size: 5860
NspI r_CATG'y
Cuts at: 1132 1229 3218 4763 1132
Size: 97 1989 1545 2229
Fragments arranged by size:
2229 1989 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5860
PciI A'CATG_T
Cuts at: 4759 4759
Size: 5860
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2389 2416 1108
Size: 1281 27 4552
Fragments arranged by size:
4552 1281 27
PfoI T'CCnGG_A
Cuts at: 2036 2036
Size: 5860
PmeI GTTT'AAAC
Cuts at: 3164 3164
Size: 5860
PmlI CAC'GTG
Cuts at: 2811 2811
Size: 5860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3929 3961 5442 5474 3929
Size: 32 1481 32 4315
Fragments arranged by size:
4315 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2791 2791
Size: 5860
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5860
PsiI TTA'TAA
Cuts at: 3430 3430
Size: 5860
PstI C_TGCA'G
Cuts at: 830 3212 830
Size: 2382 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5860
PvuII CAG'CTG
Cuts at: 2302 2302
Size: 5860
SalI G'TCGA_C
Cuts at: 1936 3202 1936
Size: 1266 4594
SbfI CC_TGCA'GG
Cuts at: 3212 3212
Size: 5860
ScaI AGT'ACT
Cuts at: 4108 5820 4108
Size: 1712 4148
SfcI C'TryA_G
Cuts at: 826 1045 2461 3064 3208 4343 5024 5215 826
Size: 219 1416 603 144 1135 681 191 1471
Fragments arranged by size:
1471 1416 1135 681 603 219 191 144
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5860
SmaI CCC'GGG
Cuts at: 1872 2012 3187 1872
Size: 140 1175 4545
Fragments arranged by size:
4545 1175 140
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2438 2705 3964 4865 5127 820
Size: 197 926 495 267 1259 901 262 1553
Fragments arranged by size:
1553 1259 926 901 495 267 262 197
SnaBI TAC'GTA
Cuts at: 493 3651 493
Size: 3158 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5860
SphI G_CATG'C
Cuts at: 1132 3218 1132
Size: 2086 3774
SspI AAT'ATT
Cuts at: 5 52 2245 3784 5
Size: 47 2193 1539 2081
Fragments arranged by size:
2193 2081 1539 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5860
StyI C'CwwG_G
Cuts at: 513 1065 2004 2834 3299 513
Size: 552 939 830 465 3074
Fragments arranged by size:
3074 939 830 552 465
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2847 3887 3904 4057 4242 4687 325
Size: 1255 1267 1040 17 153 185 445 1498
Fragments arranged by size:
1498 1267 1255 1040 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2286 2400 2457
Size: 274 80 33 51 1443 309 114 57
Cuts at: 2457 4106 5818 96
Size: 1649 1712 138
Fragments arranged by size:
1712 1649 1443 309 274 138 114 80
57 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2830 2862 5820 5852 2830
Size: 32 2958 32 2838
Fragments arranged by size:
2958 2838 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5860
XbaI T'CTAG_A
Cuts at: 3196 3196
Size: 5860
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5860
XmaI C'CCGG_G
Cuts at: 1870 2010 3185 1870
Size: 140 1175 4545
Fragments arranged by size:
4545 1175 140
XmnI GAAnn'nnTTC
Cuts at: 2069 3989 2069
Size: 1920 3940
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3668 276
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI