Restriction map for
pFN21AE0650
(Circular) (Six-base) MAPSORT of: pFN21AE0650.seq Check: 3979 from: 1 to: 5851
pFN21AE0650 5851 bp
With 182 enzymes: SgfI *
October 20, 2009 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2137 2137
Size: 5851
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3661 278
Size: 53 83 186 936 2125 2468
Fragments arranged by size:
2468 2125 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2259 3036 3194 1937
Size: 322 777 158 4594
Fragments arranged by size:
4594 777 322 158
Acc65I G'GTAC_C
Cuts at: 3172 3172
Size: 5851
AclI AA'CG_TT
Cuts at: 1563 3978 4351 1563
Size: 2415 373 3063
Fragments arranged by size:
3063 2415 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2240 3086 3914 5298 5566 1632
Size: 608 846 828 1384 268 1917
Fragments arranged by size:
1917 1384 846 828 608 268
AfeI AGC'GCT
Cuts at: 2339 2339
Size: 5851
AflII C'TTAA_G
Cuts at: 820 1017 2552 820
Size: 197 1535 4119
Fragments arranged by size:
4119 1535 197
AflIII A'CryG_T
Cuts at: 1203 2676 4750 5727 1203
Size: 1473 2074 977 1327
Fragments arranged by size:
2074 1473 1327 977
AgeI A'CCGG_T
Cuts at: 4682 4682
Size: 5851
AhdI GACnn_n'nnGTC
Cuts at: 2680 4580 2680
Size: 1900 3951
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5851
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5851
AlwNI CAG_nnn'CTG
Cuts at: 2152 2473 3594 5166 2152
Size: 321 1121 1572 2837
Fragments arranged by size:
2837 1572 1121 321
ApaI G_GGCC'C
Cuts at: 2039 2039
Size: 5851
ApaLI G'TGCA_C
Cuts at: 3908 5064 3908
Size: 1156 4695
ApoI r'AATT_y
Cuts at: 1493 3160 3386 3538 5647 1493
Size: 1667 226 152 2109 1697
Fragments arranged by size:
2109 1697 1667 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4405 160
Size: 872 3373 1606
Fragments arranged by size:
3373 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
AvaI C'yCGr_G
Cuts at: 1870 1943 2758 2958 3176 1870
Size: 73 815 200 218 4545
Fragments arranged by size:
4545 815 218 200 73
BamHI G'GATC_C
Cuts at: 3181 3548 3181
Size: 367 5484
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3172 4627 618
Size: 325 217 627 1385 1455 1842
Fragments arranged by size:
1842 1455 1385 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2039 2106 3170 1391
Size: 648 67 1064 4072
Fragments arranged by size:
4072 1064 648 67
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2867 3074 928
Size: 971 968 207 3705
Fragments arranged by size:
3705 971 968 207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2914 2948 4042 4076 1903
Size: 34 977 34 1094 34 3678
Fragments arranged by size:
3678 1094 977 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3743 4953 3743
Size: 1210 4641
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5851
BfrBI ATG'CAT
Cuts at: 2364 2364
Size: 5851
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2793 4462 136
Size: 107 122 71 631 605 1121 1669 1525
Fragments arranged by size:
1669 1525 1121 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5846 5846
Size: 5851
BlpI GC'TnA_GC
Cuts at: 3268 3268
Size: 5851
Bme1580I G_kGCm'C
Cuts at: 1790 2039 2046 2218 3912 5068 1790
Size: 249 7 172 1694 1156 2573
Fragments arranged by size:
2573 1694 1156 249 172 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5851
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2995 3582 4540
Size: 416 53 83 399 24 1578 587 958
Cuts at: 4540 442
Size: 1753
Fragments arranged by size:
1753 1578 958 587 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5851
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2201 4511 1239
Size: 962 2310 2579
Fragments arranged by size:
2579 2310 962
Bpu10I CC'TnA_GC
Cuts at: 2577 2577
Size: 5851
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3976 4841 5139 3976
Size: 865 298 4688
Fragments arranged by size:
4688 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2576 4514 882
Size: 617 1077 1938 2219
Fragments arranged by size:
2219 1938 1077 617
BsaAI yAC'GTr
Cuts at: 493 1204 3642 493
Size: 711 2438 2702
Fragments arranged by size:
2702 2438 711
BsaBI GATnn'nnATC
Cuts at: 5845 5845
Size: 5851
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3658 4040 4675 275
Size: 53 83 186 936 2125 382 635 1451
Fragments arranged by size:
2125 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4284 4682 4956 5103 5514 4284
Size: 398 274 147 411 4621
Fragments arranged by size:
4621 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5626
Fragments arranged by size:
5626 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2336 2785 3017 3077 3112 1186
Size: 853 297 449 232 60 35 3925
Fragments arranged by size:
3925 853 449 297 232 60 35
BseYI C'CCAG_C
Cuts at: 2152 5054 5550 2152
Size: 2902 496 2453
Fragments arranged by size:
2902 2453 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2459 1163
Size: 573 723 4555
Fragments arranged by size:
4555 723 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3301 4062 5090 1540
Size: 275 186 1300 761 1028 2301
Fragments arranged by size:
2301 1300 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2086 3912 3997 5068 2086
Size: 1826 85 1071 2869
Fragments arranged by size:
2869 1826 1071 85
BsmI GAATG_Cn'
Cuts at: 2366 3362 3455 2366
Size: 996 93 4762
Fragments arranged by size:
4762 996 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2046 2086 2106 2218 3170 3912
Size: 399 249 7 40 20 112 952 742
Cuts at: 3912 3997 5068 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 952 742 399 249 112 85
40 20 7
BspEI T'CCGG_A
Cuts at: 5514 5514
Size: 5851
BspHI T'CATG_A
Cuts at: 2898 3740 5470 2898
Size: 842 1730 3279
Fragments arranged by size:
3279 1730 842
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2137 3206 844
Size: 1293 1069 3489
Fragments arranged by size:
3489 1293 1069
BsrBI CCG'CTC
Cuts at: 1394 1823 3738 1394
Size: 429 1915 3507
Fragments arranged by size:
3507 1915 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2487 4346 4520 65
Size: 1183 1239 1859 174 1396
Fragments arranged by size:
1859 1396 1239 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3081 4495 4682 1700
Size: 110 1271 1414 187 2869
Fragments arranged by size:
2869 1414 1271 187 110
BsrGI T'GTAC_A
Cuts at: 96 2700 96
Size: 2604 3247
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5851
BssSI C'ACGA_G
Cuts at: 3911 4923 3911
Size: 1012 4839
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5851
BstBI TT'CG_AA
Cuts at: 4655 4655
Size: 5851
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5851
BstXI CCAn_nnnn'nTGG
Cuts at: 3019 3019
Size: 5851
BstYI r'GATC_y
Cuts at: 1972 3181 3548 3933 3950 5402 5488 5500 5846
Size: 1209 367 385 17 1452 86 12 346
Cuts at: 5846 1972
Size: 1977
Fragments arranged by size:
1977 1452 1209 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2637 2637
Size: 5851
BtgI C'CryG_G
Cuts at: 513 1065 2004 2329 513
Size: 552 939 325 4035
Fragments arranged by size:
4035 939 552 325
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5112
Fragments arranged by size:
5112 683 56
BtsI GCAGTG_nn'
Cuts at: 2124 2457 2553 3367 4161 4181 2124
Size: 333 96 814 794 20 3794
Fragments arranged by size:
3794 814 794 333 96 20
ClaI AT'CG_AT
Cuts at: 1554 2924 1554
Size: 1370 4481
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5816
DraI TTT'AAA
Cuts at: 3155 3155
Size: 5851
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4858 809
Size: 507 3542 1802
Fragments arranged by size:
3542 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3021 3298 4187
Size: 54 1425 50 275 18 1191 277 889
Cuts at: 4187 5816 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1191 889 277 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3298 1537
Size: 275 1486 4090
Fragments arranged by size:
4090 1486 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2762 3781 1353
Size: 1409 1019 3423
Fragments arranged by size:
3423 1409 1019
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2079 2837 4438 4812 4958 387
Size: 1064 138 490 758 1601 374 146 1280
Fragments arranged by size:
1601 1280 1064 758 490 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2240 3086 3914 4511 5298 5566
Size: 393 569 39 846 828 597 787 268
Cuts at: 5566 1239
Size: 1524
Fragments arranged by size:
1524 846 828 787 597 569 393 268
39
EcoO109I rG'GnC_Cy
Cuts at: 2035 2036 2641 3061 2035
Size: 1 605 420 4825
Fragments arranged by size:
4825 605 420 1
EcoRI G'AATT_C
Cuts at: 3160 3538 3160
Size: 378 5473
FspI TGC'GCA
Cuts at: 4357 4357
Size: 5851
HaeII r_GCGC'y
Cuts at: 1678 2341 4998 1678
Size: 663 2657 2531
Fragments arranged by size:
2657 2531 663
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2666 2698 2875 2907 4492
Size: 32 135 32 1094 32 177 32 1585
Cuts at: 4492 4524 4566 4598 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1585 1094 177 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3195 3441 669
Size: 988 281 1257 246 3079
Fragments arranged by size:
3079 1257 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5851
HpaI GTT'AAC
Cuts at: 3441 3441
Size: 5851
KpnI G_GTAC'C
Cuts at: 3176 3176
Size: 5851
MfeI C'AATT_G
Cuts at: 3450 3450
Size: 5851
MluI A'CGCG_T
Cuts at: 5727 5727
Size: 5851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2760 4965 5149 1305
Size: 1455 2205 184 2007
Fragments arranged by size:
2205 2007 1455 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3023 10
Size: 54 1425 343 1191 2838
Fragments arranged by size:
2838 1425 1191 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2354 2499 3809 4168 4327 518
Size: 648 1188 145 1310 359 159 2042
Fragments arranged by size:
2042 1310 1188 648 359 159 145
MspA1I CmG'CkG
Cuts at: 1606 2842 3267 3944 5092 5337 1606
Size: 1236 425 677 1148 245 2120
Fragments arranged by size:
2120 1236 1148 677 425 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2329 513
Size: 552 939 325 4035
Fragments arranged by size:
4035 939 552 325
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5851
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5851
NotI GC'GGCC_GC
Cuts at: 3298 3298
Size: 5851
NsiI A_TGCA'T
Cuts at: 2366 2366
Size: 5851
NspI r_CATG'y
Cuts at: 1132 1229 2326 2680 3209 4754 1132
Size: 97 1097 354 529 1545 2229
Fragments arranged by size:
2229 1545 1097 529 354 97
PasI CC'CwG_GG
Cuts at: 1348 2686 1348
Size: 1338 4513
PciI A'CATG_T
Cuts at: 2676 4750 2676
Size: 2074 3777
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2926 1108
Size: 1818 4033
PfoI T'CCnGG_A
Cuts at: 2304 2304
Size: 5851
PmeI GTTT'AAAC
Cuts at: 3155 3155
Size: 5851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3920 3952 5433 5465 3920
Size: 32 1481 32 4306
Fragments arranged by size:
4306 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2641 3061 2641
Size: 420 5431
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5851
PsiI TTA'TAA
Cuts at: 3421 3421
Size: 5851
PspOMI G'GGCC_C
Cuts at: 2035 2035
Size: 5851
PstI C_TGCA'G
Cuts at: 830 2437 2604 2970 3203 830
Size: 1607 167 366 233 3478
Fragments arranged by size:
3478 1607 366 233 167
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5851
PvuII CAG'CTG
Cuts at: 2842 2842
Size: 5851
SalI G'TCGA_C
Cuts at: 1936 3193 1936
Size: 1257 4594
SbfI CC_TGCA'GG
Cuts at: 3203 3203
Size: 5851
ScaI AGT'ACT
Cuts at: 4099 5811 4099
Size: 1712 4139
SexAI A'CCwGG_T
Cuts at: 3045 3147 3045
Size: 102 5749
SfcI C'TryA_G
Cuts at: 826 1045 2433 2600 2966 3199 4334 5015 5206
Size: 219 1388 167 366 233 1135 681 191
Cuts at: 5206 826
Size: 1471
Fragments arranged by size:
1471 1388 1135 681 366 233 219 191
167
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5851
SmaI CCC'GGG
Cuts at: 1872 2760 3178 1872
Size: 888 418 4545
Fragments arranged by size:
4545 888 418
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2552 3955 4856 5118 820
Size: 197 926 609 1403 901 262 1553
Fragments arranged by size:
1553 1403 926 901 609 262 197
SnaBI TAC'GTA
Cuts at: 493 3642 493
Size: 3149 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5851
SphI G_CATG'C
Cuts at: 1132 3209 1132
Size: 2077 3774
SspI AAT'ATT
Cuts at: 5 52 3775 5
Size: 47 3723 2081
Fragments arranged by size:
3723 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5851
StyI C'CwwG_G
Cuts at: 513 1065 2004 2241 2329 3018 3290 513
Size: 552 939 237 88 689 272 3074
Fragments arranged by size:
3074 939 689 552 272 237 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2138 2150 2603 2678 3878 3895 4048
Size: 1255 558 12 453 75 1200 17 153
Cuts at: 4048 4233 4678 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1200 558 453 445 185 153
75 17 12
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2700 4097 5809
Size: 274 80 33 51 1443 723 1397 1712
Cuts at: 5809 96
Size: 138
Fragments arranged by size:
1712 1443 1397 723 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5811 5843 5811
Size: 32 5819
Tth111I GACn'n_nGTC
Cuts at: 1316 2993 1316
Size: 1677 4174
XbaI T'CTAG_A
Cuts at: 3187 3187
Size: 5851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2149 2198 2149
Size: 49 5802
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5851
XmaI C'CCGG_G
Cuts at: 1870 2758 3176 1870
Size: 888 418 4545
Fragments arranged by size:
4545 888 418
XmnI GAAnn'nnTTC
Cuts at: 3980 3980
Size: 5851
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3659 276
Size: 53 83 186 936 2125 2468
Fragments arranged by size:
2468 2125 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI